Low concentrations of caffeic acid phenethyl ester stimulate osteogenesis in vitro

2021 ◽  
pp. 101618
Author(s):  
Paulo Henrique Neves Santos ◽  
Hebert Lacerda Silva ◽  
Elizabeth Ferreira Martinez ◽  
Júlio César Joly ◽  
Ana Paula Dias Demasi ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Low Concentrations ◽  
Osteogenesis In Vitro

Effect of caffeic acid phenethyl ester on gastric acid secretion in vitro

2005 ◽  
Vol 521 (1-3) ◽  
pp. 139-143 ◽  
Author(s):  
Francesca Borrelli ◽  
Inmaculada Posadas ◽  
Raffaele Capasso ◽  
Gabriella Aviello ◽  
Valeria Ascione ◽  
...  
Keyword(s):  
Acid Secretion ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester

Formulation development of folic acid conjugated PLGA nanoparticles for improved cytotoxicity of Caffeic acid phenethyl ester

2021 ◽  
Vol 09 ◽  
Author(s):  
Harshad S Kapare ◽  
Sathiyanarayanan L ◽  
Arulmozhi S ◽  
Kakasaheb Mahadik
Keyword(s):  
Folic Acid ◽  
Drug Release ◽  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Therapeutic Effects ◽  
Formulation Development ◽  
Sustained Drug Release ◽  
Active Constituent

Background: Honey bee propolis is one of the natural product reported in various traditional systems of medicines including Ayurveda. Caffeic acid phenethyl ester (CAPE) is an active constituent of propolis which is well known for its anticancer potential. The therapeutic effects of CAPE are restricted owing to its less aqueous solubility and low bioavailability. Objective: In this study CAPE loaded folic acid conjugated nanoparticle system (CLFPN) was investigated to enhance solubility, achieve sustained drug release and improved cytotoxicity of CAPE. Methods: Formulation development, characterization and optimization were carried out by design of experiment approach. In vitro and in vivo cytotoxicity study was carried out for optimized formulations. Results: Developed nanoparticles showed particle size and encapsulation efficiency of 170 ± 2 - 195 ± 3 nm and 75.66 ± 1.52 - 78.80 ± 1.25 % respectively. Optimized formulation CLFPN showed sustained drug release over a period of 42 h. GI50 concentration was decreased by 46.09% for formulation as compared to CAPE in MCF-7 cells indicating targeting effect of CLFPN. An improved in vitro cytotoxic effect was reflected in in-vivo Daltons Ascites Lymphoma model by reducing tumor cells count. Conclusion: The desired nanoparticle characteristic with improved in vivo and in vitro cytotoxicity was shown by developed formulation. Thus it can be further investigated for biomedical applications.

In Vivo and In Vitro Antıneoplastic Actions of Caffeic Acid Phenethyl Ester (CAPE): Therapeutic Perspectives

2013 ◽  
Vol 65 (4) ◽  
pp. 515-526 ◽  
Author(s):  
Sumeyya Akyol ◽  
Gulfer Ozturk ◽  
Zeynep Ginis ◽  
Ferah Armutcu ◽  
M. Ramazan Yigitoglu ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester

Potential cytoprotection: antioxidant defence by caffeic acid phenethyl ester against free radical-induced damage of lipids, DNA, and proteins

2008 ◽  
Vol 86 (5) ◽  
pp. 279-287 ◽  
Author(s):  
Ting Wang ◽  
Lixiang Chen ◽  
Weimin Wu ◽  
Yuan Long ◽  
Rui Wang
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Caffeic Acid ◽  
Erythrocyte Membrane ◽  
Polyacrylamide Gel Electrophoresis ◽  
Caffeic Acid Phenethyl Ester ◽  
Water Soluble ◽  
Pbr322 Dna ◽  
Acid Esters

Oxidative stress is considered to be a major cause of cellular injuries in a variety of chronic health problems, such as carcinogenesis and neurodegenerative disorders. Caffeic acid phenethyl ester (CAPE), derived from the propolis of honeybee hives, possesses a variety of biological and pharmacological properties including antioxidant and anticancer activity. In the present study, we focused on the diverse antioxidative functionalities of CAPE and its related polyphenolic acid esters on cellular macromolecules in vitro. The effects on human erythrocyte membrane ghost lipid peroxidation, plasmid pBR322 DNA, and protein damage initiated by the water-soluble initiator 2,2′-azobis(2-amidinopropane) hydrochloride (AAPH) and hydrogen peroxide (H2O2) were monitored by formation of hydroperoxides and by DNA nicking assay, single-cell alkaline electrophoresis, and SDS-polyacrylamide gel electrophoresis. Our results showed that CAPE and its related polyphenolic acid esters elicited remarkable inhibitory effects on erythrocyte membrane lipid peroxidation, cellular DNA strand breakage, and protein fragmentation. The results suggest that CAPE is a potent exogenous cytoprotective and antigenotoxic agent against cell oxidative damage that could be used as a template for designing novel drugs to combat diseases induced by oxidative stress components, such as various types of cancer.

In Vitro and in Vivo Stability of Caffeic Acid Phenethyl Ester, a Bioactive Compound of Propolis

2007 ◽  
Vol 55 (9) ◽  
pp. 3398-3407 ◽  
Author(s):  
Nicola Celli ◽  
Luana K. Dragani ◽  
Stefania Murzilli ◽  
Tommaso Pagliani ◽  
Andreina Poggi
Keyword(s):  
Caffeic Acid ◽  
Bioactive Compound ◽  
Caffeic Acid Phenethyl Ester

scholarly journals Novel Caffeic Acid Phenethyl Ester-Mortalin Antibody Nanoparticles Offer Enhanced Selective Cytotoxicity to Cancer Cells

Cancers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2370
Author(s):  
Jia Wang ◽  
Priyanshu Bhargava ◽  
Yue Yu ◽  
Anissa Nofita Sari ◽  
Huayue Zhang ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Caffeic Acid ◽  
Treated Group ◽  
Caffeic Acid Phenethyl Ester ◽  
Caspase Cleavage ◽  
Unique Cell

Caffeic acid phenethyl ester (CAPE) is a key bioactive ingredient of honeybee propolis and is claimed to have anticancer activity. Since mortalin, a hsp70 chaperone, is enriched in a cancerous cell surface, we recruited a unique cell internalizing anti-mortalin antibody (MotAb) to generate mortalin-targeting CAPE nanoparticles (CAPE-MotAb). Biophysical and biomolecular analyses revealed enhanced anticancer activity of CAPE-MotAb both in in vitro and in vivo assays. We demonstrate that CAPE-MotAb cause a stronger dose-dependent growth arrest/apoptosis of cancer cells through the downregulation of Cyclin D1-CDK4, phospho-Rb, PARP-1, and anti-apoptotic protein Bcl2. Concomitantly, a significant increase in the expression of p53, p21WAF1, and caspase cleavage was obtained only in CAPE-MotAb treated cells. We also demonstrate that CAPE-MotAb caused a remarkably enhanced downregulation of proteins critically involved in cell migration. In vivo tumor growth assays for subcutaneous xenografts in nude mice also revealed a significantly enhanced suppression of tumor growth in the treated group suggesting that these novel CAPE-MotAb nanoparticles may serve as a potent anticancer nanomedicine.

scholarly journals Antimicrobial and Antibiofilm Activity against Streptococcus mutans of Individual and Mixtures of the Main Polyphenolic Compounds Found in Chilean Propolis

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Jorge Jesús Veloz ◽  
Marysol Alvear ◽  
Luis A. Salazar
Keyword(s):  
Caffeic Acid ◽  
Streptococcus Mutans ◽  
Caffeic Acid Phenethyl Ester ◽  
Biological Properties ◽  
Tooth Surface ◽  
Dilution Method ◽  
Antibiofilm Activity ◽  
Biofilm Thickness ◽  
Low Concentrations ◽  
Important Health

Dental caries is multifactorial disease and an important health problem worldwide. Streptococcus mutans is considered as a major cariogenic agent in oral cavity. This bacteria can synthetize soluble and insoluble glucans from sucrose by glucosyltransferases enzymes and generate stable biofilm on the tooth surface. Biological properties of Chilean propolis have been described and it includes antimicrobial, antifungal, and antibiofilm activities. The main goal of this study was to quantify the concentrations of main flavonoids presents in Chilean propolis and compare some biological properties such as antimicrobial and antibiofilm activity of individual compounds and the mixture of this compounds, against S. mutans cultures. Chilean propolis was studied and some polyphenols present in this extract were quantified by HPLC-DAD using commercial standards of apigenin, quercetin, pinocembrin, and caffeic acid phenethyl ester (CAPE). MIC for antimicrobial activity was determined by serial dilution method and biofilm thickness on S. mutans was quantified by confocal microscopy. Pinocembrin, apigenin, quercetin, and caffeic acid phenethyl ester (CAPE) are the most abundant compounds in Chilean propolis. These polyphenols have strong antimicrobial and antibiofilm potential at low concentrations. However, pinocembrin and apigenin have a greater contribution to this action. The effect of polyphenols on S. mutans is produced by a combination of mechanisms to decrease bacterial growth and affect biofilm proliferation due to changes in their architecture.

Antimicrobial medical sutures with caffeic acid phenethyl ester and their in vitro/in vivo biological assessment

MedChemComm ◽  
2013 ◽  
Vol 4 (5) ◽  
pp. 777 ◽  
Author(s):  
Hye Sun Lee ◽  
Soo Youn Lee ◽  
So Hyun Park ◽  
Jin Hyung Lee ◽  
Sang Kook Ahn ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Biological Assessment
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