scholarly journals Antimicrobial and Antibiofilm Activity against Streptococcus mutans of Individual and Mixtures of the Main Polyphenolic Compounds Found in Chilean Propolis

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Jorge Jesús Veloz ◽  
Marysol Alvear ◽  
Luis A. Salazar
Keyword(s):  
Caffeic Acid ◽  
Streptococcus Mutans ◽  
Caffeic Acid Phenethyl Ester ◽  
Biological Properties ◽  
Tooth Surface ◽  
Dilution Method ◽  
Antibiofilm Activity ◽  
Biofilm Thickness ◽  
Low Concentrations ◽  
Important Health

Dental caries is multifactorial disease and an important health problem worldwide. Streptococcus mutans is considered as a major cariogenic agent in oral cavity. This bacteria can synthetize soluble and insoluble glucans from sucrose by glucosyltransferases enzymes and generate stable biofilm on the tooth surface. Biological properties of Chilean propolis have been described and it includes antimicrobial, antifungal, and antibiofilm activities. The main goal of this study was to quantify the concentrations of main flavonoids presents in Chilean propolis and compare some biological properties such as antimicrobial and antibiofilm activity of individual compounds and the mixture of this compounds, against S. mutans cultures. Chilean propolis was studied and some polyphenols present in this extract were quantified by HPLC-DAD using commercial standards of apigenin, quercetin, pinocembrin, and caffeic acid phenethyl ester (CAPE). MIC for antimicrobial activity was determined by serial dilution method and biofilm thickness on S. mutans was quantified by confocal microscopy. Pinocembrin, apigenin, quercetin, and caffeic acid phenethyl ester (CAPE) are the most abundant compounds in Chilean propolis. These polyphenols have strong antimicrobial and antibiofilm potential at low concentrations. However, pinocembrin and apigenin have a greater contribution to this action. The effect of polyphenols on S. mutans is produced by a combination of mechanisms to decrease bacterial growth and affect biofilm proliferation due to changes in their architecture.

scholarly journals Antibiofilm Activity of Mangosteen (Garcinia mangostana L.) Flavonoids against Streptococcus mutans Bacteria

2020 ◽  
Vol 10 (2) ◽  
pp. 48
Author(s):  
Sri Kunarti ◽  
Aulia Ramadhani ◽  
Laskmiari Setyowati
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Tooth Enamel ◽  
Tooth Surface ◽  
Control Group ◽  
Antibiofilm Activity ◽  
Garcinia Mangostana ◽  
Control Group Design ◽  
Biofilm Inhibition ◽  
Significant Difference

Background: Dental caries is one of the most common infectious diseases and often occurs in the community caused by bacteria. Attached bacteria in the tooth surface for a long time will form a biofilm and will lead to demineralization characterized by damage in the structure of the tooth enamel. The bacteria that cause dental caries and can form biofilms is Streptococcus mutans. The bacteria inside biofilms are more resistant to antibacterial agents. Flavonoids in mangosteen pericarp extract can be a cleaner alternative for the anti-biofilm cavity that has properties against Streptococcus mutans. Purpose: To determine the activity of flavonoids in mangosteen pericarp extract at a certain concentration against Streptococcus mutans bacteria. Methods: This study was a laboratory experimental study with a post-test only control group design. Streptococcus mutans were diluted according to the Mc Farland dilution standard 106 in Tryptic Soy Broth (TSB) medium and put in a flexible U-bottom microtiter plate. Then it was incubated for 5x24 hours and checked using crystal violet simple staining to see the formation of biofilms. Flavonoid extract of mangosteen pericarp performed serial dilution in a concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78% was added, and the incubation process were conducted for 1x24 hours. OD (Optical Density) readings were done with a wavelength of 595 nm. Results: There was a significant difference between the test groups and the positive control group. The concentration of 100% had the anti-biofilm activity and showed the value of the highest percentage of inhibition, whilst the concentration of 0.78% showed a minimum biofilm inhibition concentration. The results were demonstrated by a statistical analysis test. Conclusion: Flavonoid extract of mangosteen pericarp at a certain concentration has anti-biofilm activity against Streptococcus mutans biofilm.

Low concentrations of caffeic acid phenethyl ester stimulate osteogenesis in vitro

2021 ◽  
pp. 101618
Author(s):  
Paulo Henrique Neves Santos ◽  
Hebert Lacerda Silva ◽  
Elizabeth Ferreira Martinez ◽  
Júlio César Joly ◽  
Ana Paula Dias Demasi ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Low Concentrations ◽  
Osteogenesis In Vitro

scholarly journals Antibiofilm Activity of Chilean Propolis onStreptococcus mutansIs Influenced by the Year of Collection

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Jorge Jesús Veloz ◽  
Nicolás Saavedra ◽  
Alexis Lillo ◽  
Marysol Alvear ◽  
Leticia Barrientos ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Antibacterial Effect ◽  
Inhibitory Effect ◽  
Biological Properties ◽  
Antibiofilm Activity ◽  
Total Polyphenols

The chemical composition of propolis varies according to factors that could have an influence on its biological properties. Polyphenols from propolis have demonstrated an inhibitory effect onStreptococcus mutansgrowth. However, it is not known if different years of propolis collection may affect its activity. We aimed to elucidate if the year of collection of propolis influences its activity onStreptococcus mutans. Polyphenol-rich extracts were prepared from propolis collected in three different years, characterized by LC-MS and quantified the content of total polyphenols and flavonoids groups. Finally, was evaluated the antibacterial effect onStreptococcus mutansand the biofilm formation. Qualitative differences were observed in total polyphenols, flavones, and flavonols and the chemical composition between the extracts, affecting the strength of inhibition of biofilm formation but not the antimicrobial assays. In conclusion, chemical composition of propolis depends on the year of collection and influences the strength of the inhibition of biofilm formation.

scholarly journals Caffeic Acid Phenethyl Ester (CAPE) Induced Apoptosis in Serous Ovarian Cancer OV7 Cells by Deregulation of BCL2/BAX Genes

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3514 ◽  
Author(s):  
Anna Kleczka ◽  
Robert Kubina ◽  
Radosław Dzik ◽  
Krzysztof Jasik ◽  
Jerzy Stojko ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Biological Properties ◽  
New Drugs ◽  
Pcr Analysis ◽  
Ovarian Cancer Cells ◽  
Cell Nuclei ◽  
Anticancer Properties ◽  
Induced Apoptosis

Ovarian cancer has the worst prognosis among all gynecological cancers. Therefore, it seems reasonable to seek new drugs that may improve the effectiveness of treatment or mitigate the adverse effects of chemotherapy. Caffeic acid phenethyl ester (CAPE) has many beneficial biological properties. The aim of the study was to assess the anticancer properties of CAPE against serum ovarian carcinoma cells. The morphology of the cells was evaluated in H-E staining and in transmission electron microscopy. The cytotoxic and proapoptotic activity of CAPE was investigated by using the XTT-NR-SRB assay, qRT-PCR analysis of BAX/BCL2 expression, and by cytometric evaluation. CAPE causes constriction in OV7 cells, numerous granulomas were observed in the cytoplasm, the cell nuclei were pyknotic. Autophagosomal vacuoles could suggest the occurrence of aponecrosis. CAPE significantly decreased the lysosomal activity and the total synthesis of cellular proteins. CAPE exhibited, dose and time dependent, cytotoxic activity against OV7 serum ovarian cancer cells. In OV7 cells CAPE induced apoptosis via dysregulation of BAX/BCL2 balance, while activated proapoptotic BAX gene expression level was 10 times higher than BCL2.

scholarly journals Antibacterial Effect of Caffeic Acid Phenethyl Ester on Cariogenic Bacteria and Streptococcus mutans Biofilms

2020 ◽  
Vol 64 (9) ◽  
Author(s):  
Yumei Niu ◽  
Kun Wang ◽  
Sainan Zheng ◽  
Yufei Wang ◽  
Qian Ren ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Streptococcus Mutans ◽  
Acid Tolerance ◽  
Caffeic Acid Phenethyl Ester ◽  
Inhibitory Effect ◽  
Extracellular Polysaccharides ◽  
Common Disease ◽  
Streptococcus Sobrinus ◽  
Content Type ◽  
Cariogenic Bacteria

ABSTRACT Dental caries is the most common disease in the human mouth. Streptococcus mutans is the primary cariogenic bacterium. Propolis is a nontoxic natural product with a strong inhibitory effect on oral cariogenic bacteria. The polyphenol-rich extract from propolis inhibits S. mutans growth and biofilm formation, as well as the genes involved in virulence and adherence, through the inhibition of glucosyltransferases (GTF). However, because the chemical composition of propolis is highly variable and complex, the mechanism of its antimicrobial action and the active compound are controversial and not completely understood. Caffeic acid phenethyl ester (CAPE) is abundant in the polyphenolic compounds from propolis, and it has many pharmacological effects. In this study, we investigated the antibacterial effects of CAPE on common oral cariogenic bacteria (Streptococcus mutans, Streptococcus sobrinus, Actinomyces viscosus, and Lactobacillus acidophilus) and its effects on the biofilm-forming and cariogenic abilities of S. mutans. CAPE shows remarkable antimicrobial activity against cariogenic bacteria. Moreover, CAPE also inhibits the formation of S. mutans biofilms and their metabolic activity in mature biofilms. Furthermore, CAPE can inhibit the key virulence factors of S. mutans associated with cariogenicity, including acid production, acid tolerance, and the bacterium’s ability to produce extracellular polysaccharides (EPS), without affecting bacterial viability at subinhibitory levels. In conclusion, CAPE appears to be a new agent with anticariogenic potential, not only via inhibition of the growth of cariogenic bacteria.

scholarly journals Antifungal Activity of the Phenolic Compounds Ellagic Acid (EA) and Caffeic Acid Phenethyl Ester (CAPE) against Drug-Resistant Candida auris

2021 ◽  
Vol 7 (9) ◽  
pp. 763
Author(s):  
Fernanda Cristina Possamai Rossatto ◽  
Nagendran Tharmalingam ◽  
Iliana E. Escobar ◽  
Pedro Alves d’Azevedo ◽  
Karine Rigon Zimmer ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Ellagic Acid ◽  
Galleria Mellonella ◽  
Treatment Options ◽  
Caffeic Acid Phenethyl Ester ◽  
Biological Properties ◽  
Current Treatment ◽  
Drug Resistant ◽  
Candida Auris ◽  
Fungal Cell

Candida auris is an emerging healthcare-associated fungal pathogen that has become a serious global health threat. Current treatment options are limited due to drug resistance. New therapeutic strategies are required to target this organism and its pathogenicity. Plant polyphenols are structurally diverse compounds that present a vast range of biological properties. In the present study, plant-derived molecules ellagic acid (EA) and caffeic acid phenethyl ester (CAPE) were investigated for their antifungal and antivirulence activities against Candida auris. We also tested against C. albicans. The minimum inhibitory concentration (MIC) for EA ranged from 0.125 to 0.25 µg/mL and for CAPE ranged from 1 to 64 µg/mL against drug-resistant C. auris strains. Killing kinetics determined that after 4 h treatment with CAPE, there was a complete reduction of viable C. auris cells compared to fluconazole. Both compounds might act by modifying the fungal cell wall. CAPE significantly reduced the biomass and the metabolic activity of C. auris biofilm and impaired C. auris adhesion to cultured human epithelial cells. Furthermore, both compounds prolonged the survival rate of Galleria mellonella infected by C. auris (p = 0.0088 for EA at 32 mg/kg and p = 0.0028 for CAPE at 4 mg/kg). In addition, EA at 4 μg/mL prolonged the survival of C. albicans-infected Caenorhabditis elegans (p < 0.0001). CAPE was not able to prolong the survival of C. albicans-infected C. elegans. These findings highlight the antifungal and antivirulence effects of EA and CAPE against C. auris, and warrant further investigation as novel antifungal agents against drug-resistant infections.

scholarly journals The Pluripotent Activities of Caffeic Acid Phenethyl Ester

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1335
Author(s):  
Batoryna Olgierd ◽  
Żyła Kamila ◽  
Banyś Anna ◽  
Morawiec Emilia
Keyword(s):  
Wound Healing ◽  
Caffeic Acid ◽  
Honey Bee ◽  
Broad Spectrum ◽  
Caffeic Acid Phenethyl Ester ◽  
Biological Properties ◽  
Published Data ◽  
Compound A ◽  
Inflammatory Agent ◽  
Anti Cancer

Caffeic acid phenethyl ester (CAPE) is a strong antioxidant extracted from honey bee-hive propolis. The mentioned compound, a well-known NF-κB inhibitor, has been used in traditional medicine as a potent anti-inflammatory agent. CAPE has a broad spectrum of biological properties including anti-viral, anti-bacterial, anti-cancer, immunomodulatory, and wound-healing activities. This review characterizes published data about CAPE biological properties and potential therapeutic applications, that can be used in various diseases.

scholarly journals Evaluation of Anti-Biofilm Activity of Virgin Coconut Oil (Avco) Against Streptococcus Mutans ATCC 2157

2020 ◽  
Vol 18 (2) ◽  
Author(s):  
Ummi Aqilah Haron ◽  
Zurainie Abllah
Keyword(s):  
Fatty Acids ◽  
Streptococcus Mutans ◽  
Coconut Oil ◽  
Laser Scanning Microscopy ◽  
Tooth Surface ◽  
Confocal Laser ◽  
Antibiofilm Activity ◽  
Virgin Coconut Oil ◽  
Biofilm Inhibition ◽  
Significant Difference

Introduction: Dental caries has remained a major oral health problem worldwide.  Streptococcus mutans  is considered as a vital cariogenic agent in the oral cavity. It can synthesise soluble and insoluble glucans from sucrose by glucosyltransferases enzymes and develops stable biofilm on the tooth surface. In the previous study, the fatty acids profile of activated virgin coconut oil (AVCO) have been described, and it includes the excellent antimicrobial activity of the oil. The current study aims to evaluate the antibiofilm effect of AVCO against the  Streptococcus mutans  ATCC 2157 biofilm in vitro. Materials and method: The minimum biofilm inhibition concentration (MBIC) and the minimum biofilm eradication concentration (MBEC) for antibiofilm activity were determined by serial dilution method, and biofilm thickness on  S. mutans  was quantified by confocal laser scanning microscopy (CLSM).  Results:  At the concentra-tion of 15.63 mg/ml of AVCO, it successfully inhibits the development of  S. mutans  ATCC 21575 biofilm and AVCO (62.52 mg/ml) eradicate biofilm that was formed by the bacteria. The z-stack images obtained from CLSM allows the construction of 3-D biofilm structure and a significant difference in the thickness of  S. mutans  ATCC 21575 biofilm pre and post-treatment with AVCO were observed.  Conclusion:  AVCO showed good potential as anticaries where it possesses the ability to inhibits and eradicate  S. mutans  ATCC 21575 biofilm. A future study to evaluates the interaction of individuals fatty acids present in AVCO against proteins that relate to biofilm formation of  S. mutans  can be performed utilising the molecular docking tools.

Novel Caffeic Acid Phenethyl Ester (Cape) Analogues as Inducers of Heme Oxygenase-1

2017 ◽  
Vol 23 (18) ◽  
Author(s):  
Valeria Pittala ◽  
Luca Vanella ◽  
Loredana Salerno ◽  
Claudia Di Giacomo ◽  
Rosaria Acquaviva ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Heme Oxygenase ◽  
Caffeic Acid Phenethyl Ester ◽  
Heme Oxygenase 1
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