Relation of hypertriglyceridemia to plasma concentrations of biochemical markers of inflammation and endothelial activation (C-reactive protein, interleukin-6, soluble adhesion molecules, von Willebrand factor, and endothelin-1)

Abstract Dengue virus causes febrile illnesses: Dengue Fever (DF) and less frequently a life-threatening illness, Dengue Hemorrhagic Fever (DHF). The pathophysiology of hemostatic defect in dengue infection is thought to relate to the direct effect of virus or cytokines on endothelial activation. To study the state of endothelial activation during dengue infection, we measured plasma levels of von Willebrand factor antigen (vWF:Ag), vWF-collagen binding assay (vWF:CBA), and ADAMTS 13 activity in 42 children (20 with DF and 22 with DHF) during 3 phases of illness: febrile, toxic, and convalescent phase. 38 healthy children comprised as controls. Our data shows that both VWF:Ag and vWF:CBA levels were significantly higher in dengue patients (p ≤ 0.001 in both DF and DHF patients) versus controls. DHF patients had significantly higher of VWF: Ag (p = 0.01, versus DF). ADAMTS 13 activity levels were significantly decreased only in DHF patients during 3 phases of the illness (febrile; mean 78%; p = 0.016, toxic; mean 68%; p<0.001 and convalescent; mean 69%; p<0.001 compared to mean 104% of the controls). Compared to DF patients, DHF patients had significantly lower plasma concentrations of ADAMTS 13 activity during the febrile, toxic and convalescent phase (p = 0.039, p = 0.002 and p =0.003, respectively). Endothelial cell activation is a hallmark of dengue infection especially in DHF patients; indicated by a significant rise in VWF:Ag and vWF:CBA and a reciprocal decline in ADAMTS 13 activity.

Download Full-text

von Willebrand Factor, C-Reactive Protein, and 5-Year Mortality in Diabetic and Nondiabetic Subjects

Arteriosclerosis Thrombosis and Vascular Biology ◽

10.1161/01.atv.19.12.3071 ◽

1999 ◽

Vol 19 (12) ◽

pp. 3071-3078 ◽

Cited By ~ 194

Author(s):

Agnes Jager ◽

Victor W. M. van Hinsbergh ◽

Piet J. Kostense ◽

Jef J. Emeis ◽

John S. Yudkin ◽

...

Keyword(s):

Von Willebrand Factor ◽

C Reactive Protein ◽

Reactive Protein ◽

Factor C ◽

Von Willebrand ◽

Willebrand Factor

Download Full-text

Von Willebrand Factor, Plasminogen Activator Inhibitor-1 and C-Reactive Protein Are Markers of Thrombolytic Efficacy in Acute Myocardial Infarction

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646343 ◽

1992 ◽

Vol 68 (06) ◽

pp. 678-682 ◽

Cited By ~ 11

Author(s):

F Andreotti ◽

D R Hackett ◽

A W Haider ◽

M C Roncaglioni ◽

G J Davies ◽

...

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Plasminogen Activator ◽

Von Willebrand Factor ◽

Plasminogen Activator Inhibitor ◽

C Reactive Protein ◽

Reactive Protein ◽

Von Willebrand ◽

Willebrand Factor ◽

Activator Inhibitor

SummaryPlasma von Willebrand factor, plasminogen activator inhibitor activity and C-reactive protein were assessed as markers of coronary recanalisation in 30 patients with acute myocardial infarction receiving tissue-type plasminogen activator (t-PA). Blood samples were taken before t-PA (time 0), 4-hourly for 24 h and daily up to 72 h. A continuous electrocardiogram was recorded in the first 24 h. Coronary arteriography was performed 90 min and 24 h after the start of t-PA. Patients with a patent infarct artery (n = 17), compared to those with occluded artery (n = 13), showed a fall in von Willebrand factor from 0 to 24 h (p = 0.001), a greater fall in plasminogen activator inhibitor from 24 to 48 h (p = 0.04) and a fall in C-reactive protein from 48 to 72 h (p = 0.002). The accuracy of these indices compared favourably with time to peak plasma MB creatine kinase and ≥ 50% resolution of maximal ST-deviation on the electrocardiogram.Thus, changes in plasma von Willebrand factor, plasminogen activator inhibitor and C-reactive protein during the first 3 days of myocardial infarction are indicative of thrombolytic efficacy. Their concordant behaviour may reflect a common regulatory mechanism.

Download Full-text

Higher Plasma Levels of von Willebrand Factor and C-Reactive Protein during a Peritoneal Dialysis Regimen with Less Glucose and Glucose Degradation Products

Peritoneal Dialysis International ◽

10.3747/pdi.2012.00097 ◽

2013 ◽

Vol 33 (2) ◽

pp. 208-212 ◽

Cited By ~ 2

Author(s):

Caatje Y. le Poole ◽

Casper G. Schalkwijk ◽

Tom Teerlink ◽

Rob M. Valentijn ◽

Piet M. Ter Wee ◽

...

Keyword(s):

Peritoneal Dialysis ◽

Von Willebrand Factor ◽

Plasma Levels ◽

Degradation Products ◽

C Reactive Protein ◽

Reactive Protein ◽

Glucose Degradation Products ◽

Von Willebrand ◽

Willebrand Factor

Download Full-text

Acute von Willebrand Factor Secretion from the Endothelium In Vivo: Assessment through Plasma Propeptide (vWf:AgII) Levels

Thrombosis and Haemostasis ◽

10.1055/s-0038-1655973 ◽

1997 ◽

Vol 77 (02) ◽

pp. 387-393 ◽

Cited By ~ 43

Author(s):

Ulrich M Vischer ◽

Jørgen Ingerslev ◽

Claes B Wollheim ◽

Jean-Claude Mestries ◽

Dimitrios A Tsakiris ◽

...

Keyword(s):

Von Willebrand Factor ◽

Time Course ◽

Plasma Concentrations ◽

Vascular Wall ◽

Endothelial Activation ◽

Relative Increase ◽

Von Willebrand ◽

Willebrand Factor

SummaryElevated plasma concentrations of von Willebrand factor (vWf) are increasingly recognized as a cardiovascular risk factor, and are used as a marker of endothelial activation. However, the factors which determine the rate of vWf release from the endothelium in vivo have not been defined clearly. In addition, vWf plasma levels may also be influenced by adhesion of vWf to the vascular wall or to platelets, and by its rate of degradation. The propeptide of vWf (also called vWf:AgII) is stored and released in equimolar amounts with vWf. In the present study we attempted to determine whether this propeptide could be a more reliable marker of endothelial secretion than vWf itself. To accomplish this we developed an ELISA based on monoclonal antibodies. The propeptide levels in normal plasma were found to be 0.7 µg/ml, more than 10 times lower than vWf itself. Administration of desmopressin (DDAVP) induced a rapid relative increase in propeptide (from 106 to 879%) and in vWf (from 112 to 272%). However, the increases in vWf and propeptide were equivalent when expressed in molar units. A time course study indicated a half-life of the propeptide of 3 h or less. In a baboon model of disseminated intravascular coagulation (DIC) induced by FXa, vWf increased by less than 100%, whereas the propeptide concentrations increased by up to 450%. In view of the massive thrombin generation (as assessed by fibrinogen depletion), the increases in vWf are small, compared to the strong secretory response to thrombin and fibrin previously observed in vitro. Our results suggest that due to its rapid turnover, the propeptide could provide a sensitive plasma marker of acute endothelial secretion.

Download Full-text