Protein carbonyl groups as biomarkers of oxidative stress

2003 ◽  
Vol 329 (1-2) ◽  
pp. 23-38 ◽  
Author(s):  
Isabella Dalle-Donne ◽  
Ranieri Rossi ◽  
Daniela Giustarini ◽  
Aldo Milzani ◽  
Roberto Colombo
Keyword(s):  
Oxidative Stress ◽  
Protein Carbonyl ◽  
Carbonyl Groups ◽  
Protein Carbonyl Groups ◽  
Biomarkers Of Oxidative Stress

scholarly journals Protein carbonyl group content in patients affected by familiar chronic nail candidiasis

2003 ◽  
Vol 12 (4) ◽  
pp. 247-249 ◽  
Author(s):  
S. Gangemi ◽  
A. Saija ◽  
A. Tomaino ◽  
F. Cimino ◽  
R. A. Merendino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Defense Mechanisms ◽  
Protein Carbonyl ◽  
Intercellular Adhesion Molecule ◽  
Carbonyl Groups ◽  
Intercellular Adhesion Molecule 1 ◽  
Protein Carbonyl Groups ◽  
Halide System ◽  
Reactive Nitrogen Intermediates ◽  
Hands And Feet

Familiar chronic nail candidiasis (FCNC) is a rare disorder characterized by early-onset infections caused by different species of Candida, restricted to the nail of the hands and feet, and associated with a low serum concentration of intercellular adhesion molecule 1. Host defense mechanisms against candidiasis require the cooperation of many immune cells through several candidacidal mechanisms, including oxygen-dependent killing mechanisms, mediated by a superoxide anion radical myeloperoxidase-H2O2-halide system, and reactive nitrogen intermediates. We analyzed protein carbonyl groups (considered a useful marker of oxidative stress) in the serum of patients belonging to a five-generation Italian family with an isolated form of FCNC.Serum protein carbonyl groups in FCNC patients were significantly lower than those measured in healthy donors.Also, if this hypothesis is merely speculative, we could suggest that the decreased circulating level of protein carbonyl groups in these patients is not a marker of a lower oxidative stress condition, but might be linked to a lower protease activity.

Activities of brain antioxidant enzymes, lipid and protein peroxidation

Open Medicine ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. 588-594 ◽  
Author(s):  
Anna Łukaszewicz-Hussain
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Protein Carbonyl ◽  
Organophosphate Pesticides ◽  
Carbonyl Groups ◽  
Chronic Intoxication ◽  
Protein Carbonyl Groups ◽  
Protein Peroxidation ◽  
Antioxidant Enzymes Activities

AbstractOrganophosphate pesticides are known to induce oxidative stress and cause oxidative tissue damage, as has been reported in studies concerning acute and chronic intoxication with these compounds.Our objective was to investigate the activities of brain antioxidant enzymes and malonyldialdehyde, as well as the level of carbonyl groups, in rats sub-chronically intoxicated with chlorpyrifos at doses of 0.2, 2 and 5 mg per kg of body weight per day. It was found that chlorpyrifos induces change in brain antioxidant enzymes, such as superoxide dismutase, catalase and glutathione peroxidise, but to a different degree in comparison to proper control values; however, the elevated antioxidant enzymes activities failed to check lipid and protein peroxidation in the brains of rats. Thus, in sub-chronic intoxication with chlorpyrifos, as evidenced by increased level of malonyldialdehyde and carbonyl groups, oxidative stress is induced.Measurements of protein carbonyl groups appeared to give more consistent responses in the rats’ brains when compared to the malonyldialdehyde level after sub-chronic chlorpyrifos treatment.

Oxidative stress in the testis of hyperglycemic rabbits treated with repaglinide

2007 ◽  
Vol 2 (4) ◽  
pp. 538-546
Author(s):  
Anna Gumieniczek ◽  
Hanna Hopkała ◽  
Marcin Pruchniak
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Protective Effect ◽  
Protein Carbonyl ◽  
Carbonyl Groups ◽  
Protein Carbonyl Groups ◽  
Diabetic Rabbits

AbstractIn the present study, the induction of oxidative stress was examined in the testis of alloxan-induced diabetic rabbits. In addition, the protective effect of repaglinide, an oral anti-diabetic, at a dose of 1 mg daily was studied after four and eight weeks of the treatment. For these purposes, the levels of superoxide dismutase (Cu,Zn-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), glutathione (GSH), ascorbic acid (AA), lipid peroxidation products (LPO) and protein carbonyl groups (PCG) were quantified. Hyperglycemia resulted in significant increases in the antioxidative enzymes, Cu, Zn-SOD, CAT, GSH-Px, and GSSG-R after four and eight weeks, respectively. There was also an increase in GSH level, and a decrease in the level of AA. These effects were accompanied by an elevation in testicular LPO levels and PCG levels. Repaglinide was found to normalize the activity of GSSG-R and levels of GSH and AA, and blunted the increased lipid peroxidation, however no decrease in PCG levels were observed. In conclusion, some oxidative changes provoked in the testis of rabbits by hyperglycemia, were found to be reduced with repaglinide treatment at therapeutic dose.

scholarly journals PSXI-14 Supplementation with a blend containing Baccharis dracunculifolia and Tamarindus indica improve the oxidative state of steers in finishing phase

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 400-401
Author(s):  
Vicente A Diaz Avila ◽  
Edineia Bonin ◽  
Venício M Carvalho ◽  
Lucas Stafuza Moreira ◽  
Jurandir Fernando Comar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
Oxidative Status ◽  
Carbonyl Groups ◽  
Tamarindus Indica ◽  
Plasma Parameters ◽  
Protein Carbonyl Groups ◽  
Reducing Ability ◽  
Oxidative State

Abstract Tissue oxidative stress has been associated with low productivity of beef cattle. Supplementation of animals with natural antioxidants added to the diet may be an alternative to improve the tissue oxidative status and the productivity of steers at finishing phase. The present study evaluated whether the addition to the diet of a blend (Mix) containing Baccharis dracunculifolia (40%), Tamarindus indica seed (40%), cashew nutshell oil (10%) and clove oil (10%) modifies the oxidative state in the plasma and liver of steers (Angus x Zebu) in finishing phase. Forty animals were randomly distributed into four groups (ten animal per group), which received Mix respectively at the daily dose of 0.0, 2.0, 4.0 and 6.0 g per animal during 84 days. Blood and liver were taken at slaughter of the animals. Blood samples were also taken before starting the supplementation and served as control. The ferric reducing ability of plasma (FRAP), thiol groups and protein carbonyl groups were measured as plasma parameters of oxidative stress. The levels of thiobarbituric acid reactive substances (TBARS), protein carbonyl groups, reactive oxygen species (ROS), glutathione and the activity of catalase and superoxide dismutase (SOD) were measured as hepatic parameter of oxidative stress. Plasma levels of protein carbonyl groups were 30% lower in the animals supplemented with 4.0 and 6.0 g Mix and FRAP was 20% higher in the animals supplemented with 6.0 g Mix (P < 0.05; compared to the 0.0 g). Hepatic levels of TBARS were 38% lower in animals supplemented with 6.0 g Mix (P < 0.05). The other parameters were not modified. In conclusion, the supplementation of steers with Mix improved the systemic oxidative status and may be a complementary alternative to the diet of these animals in the termination phase.

scholarly journals Oxidation state of tissue thiol groups and content of protein carbonyl groups in chickens with inherited muscular dystrophy

1989 ◽  
Vol 260 (2) ◽  
pp. 359-364 ◽  
Author(s):  
M E Murphy ◽  
J P Kehrer
Keyword(s):  
Oxidative Stress ◽  
Muscular Dystrophy ◽  
Pectoralis Major Muscle ◽  
Indirect Evidence ◽  
Protein Carbonyl ◽  
Pectoralis Major ◽  
Carbonyl Groups ◽  
Thiol Groups ◽  
Protein Carbonyl Groups ◽  
Protein Pool

Indirect evidence suggests that oxidative stress may play a role in the pathogenesis of inherited muscular dystrophy, but the significance and precise extent of this contribution is poorly understood. Compared with normal muscle, significantly higher contents of glutathione, glutathione disulphide, protein-glutathione mixed disulphides and protein carbonyl groups, and significantly lower contents of free protein thiol groups, were found in pectoralis major muscle of genetically dystrophic chickens (the muscle affected by this disease) at 4 weeks of age. Other tissues did not show such marked disease-related differences. Interestingly, the protein pool in normal, but not dystrophic, pectoralis major muscle was relatively less oxidized in relation to the glutathione pool as compared with other tissues studied. The mechanisms by which this unique relationship between the thiol pools is maintained remain unknown. Although the physiological consequences of the increased content of protein carbonyl groups and the altered thiol pools in dystrophic muscle are not clear, the changes evident at such a young age are consistent with the occurrence of oxidative stress and may reflect significant damage to cellular proteins in this disease.

scholarly journals Oxidative Stress Parameters in the Liver of Growing Male Rats Receiving Various Alcoholic Beverages

Nutrients ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 158 ◽  
Author(s):  
Aleksandra Kołota ◽  
Dominika Głąbska ◽  
Michał Oczkowski ◽  
Joanna Gromadzka-Ostrowska
Keyword(s):  
Oxidative Stress ◽  
Animal Model ◽  
Alcohol Consumption ◽  
Protein Carbonyl ◽  
Alcoholic Beverages ◽  
Control Group ◽  
Carbonyl Groups ◽  
Liver Inflammation ◽  
Protein Carbonyl Groups ◽  
Markers Of Oxidative Stress

Typical alcohol consumption begins in the adolescence period, increasing the risk of alcoholic liver disease (ALD) in adolescents and young adults, and while the pathophysiology of ALD is still not completely understood, it is believed that oxidative stress may be the major contributor that initiates and promotes the progression of liver damage. The aim of the present study was to assess the influence of alcohol consumption on the markers of oxidative stress and liver inflammation in the animal model of prolonged alcohol consumption in adolescents using various alcoholic beverages. In a homogenic group of 24 male Wistar rats (4 groups—6 animals per group), since 30th day of life, in order to mimic the alcohol consumption since adolescence, animals received (1) no alcoholic beverage (control group), (2) ethanol solution, (3) red wine, or (4) beer (experimental groups) for 6 weeks. Afterwards, the activities of alcohol dehydrogenase (ADH), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), as well as levels of cytochrome P450-2E1 (CYP2E1), thiobarbituric acid-reactive substances (TBARS), protein carbonyl groups, tumor necrosis factor-α (TNF-α), and interleukine-10 (IL-10) were measured in liver homogenates. The difference between studied groups was observed for CYP2E1 and protein carbonyl groups levels (increased levels for animals receiving beer compared with control group), as well as for ALT activity (decreased activity for animals receiving beer compared with other experimental groups) (p < 0.05). The results suggested that some components of beer, other than ethanol, are responsible for its influence on the markers of oxidative stress and liver inflammation observed in the animal model of prolonged alcohol consumption in adolescents. Taking this into account, beer consumption in adolescents, which is a serious public health issue, should be assessed in further studies to broaden the knowledge of the progression of liver damage caused by alcohol consumption in this group.

scholarly journals Aronia melanocarpaTreatment and Antioxidant Status in Selected Tissues in Wistar Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Renata Francik ◽  
Mirosław Krośniak ◽  
Ilona Sanocka ◽  
Henryk Bartoń ◽  
Tomasz Hebda ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Brain Tissue ◽  
High Fat Diet ◽  
Protein Carbonyl ◽  
Carbonyl Groups ◽  
High Fat ◽  
Stress Markers ◽  
Protein Carbonyl Groups ◽  
Tissue Homogenates ◽  
The Impact

Aroniajuice is considered to be a source of compounds with high antioxidative potential. We conducted a study on the impact of compounds in theAroniajuice on oxidative stress in plasma and brain tissues. The influence ofAroniajuice on oxidative stress parameters was tested with the use of a model with a high content of fructose and nonsaturated fats. Therefore, the activity of enzymatic (catalase, CAT, and paraoxonase, PON) and nonenzymatic (thiol groups, SH, and protein carbonyl groups, PCG) oxidative stress markers, which indicate changes in the carbohydrate and protein profiles, was marked in brain tissue homogenates. AddingAroniacaused statistically significant increase in the CAT activity in plasma in all tested diets, while the PON activity showed a statistically significant increase only in case of high fat diet. In animals fed withAroniajuice supplemented with carbohydrates or fat, statistically significant increase in the PON activity and the decrease in the CAT activity in brain tissue were observed. In case of the high fat diet, an increase in the number of SH groups and a decrease in the number of PCG groups in brain tissue were observed.

scholarly journals Behaviour of protein carbonyl groups in juvenile myocardial infarction

2013 ◽  
Vol 53 (4) ◽  
pp. 297-302 ◽  
Author(s):  
Gregorio Caimi ◽  
Baldassare Canino ◽  
Egle Incalcaterra ◽  
Eleonora Ferrera ◽  
Maria Montana ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Protein Carbonyl ◽  
Carbonyl Groups ◽  
Protein Carbonyl Groups

scholarly journals Analysis of Association between Intake of Red Wine Polyphenols and Oxidative Stress Parameters in the Liver of Growing Male Rats

2020 ◽  
Vol 10 (18) ◽  
pp. 6389
Author(s):  
Aleksandra Kołota ◽  
Dominika Głąbska ◽  
Michał Oczkowski ◽  
Joanna Gromadzka-Ostrowska
Keyword(s):  
Red Wine ◽  
Sinapic Acid ◽  
Protein Carbonyl ◽  
Male Rats ◽  
Carbonyl Groups ◽  
Coumaric Acid ◽  
Oxidative Stress Parameters ◽  
Protein Carbonyl Groups ◽  
Stress Parameters ◽  
Oxidative Effect

Red wine is a complex alcoholic beverage containing various substances, including those of a potential health-promoting properties, such as polyphenols, responsible for the sensory features of wine. Their potential positive influence is associated with possibility of antioxidative, anti-inflammatory, anticancer, immunomodulating, and antiallergenic effects, but at the same time, there is a possibility of their pro-oxidative effect. In spite of the fact that in adolescence alcohol may be highly damaging, as it may disturb the development, it is abused frequently. The aim of the study was to verify the association between red wine consumption as well as dietary polyphenol intake with wine and the oxidative stress parameters in the liver of growing male rats. The growing male Wistar rats were studied, while divided into three groups of six animals, receiving red wine in a solution characterized by 10% of ethanol content since 30th day of life, for two, four, and six weeks. The alcohol intake was controlled and the content of total phenolic compounds, selected phenolic acids (hydroxybenzoic acid, caffeic acid, p-coumaric acid, ferulic acid, and sinapic acid), total anthocyanidins and selected anthocyanins (cyanidin, cyanidin 3-glucoside, cyanidin 3-rutinoside, delphinidin, and pelargonidin) were assessed. At the same time, for the liver homogenates, the levels of Thiobarbituric Acid Reactive Substances (TBARS) and protein carbonyl groups were verified. The analysis revealed statistically significant influence of red wine intake, as well as of supply of phenolic acids (hydroxybenzoic acid, caffeic acid, p-coumaric acid, ferulic acid, and sinapic acid), and anthocyanins (cyanidin, cyanidin 3-glucoside, cyanidin 3-rutinoside, delphinidin, and pelargonidin) on TBARS and protein carbonyl groups level (R > 0.80; p < 0.05), but the influence was stated only for the period of two weeks. The obtained results may suggest pro-oxidative effect of both ethanol and polyphenols for the liver of the growing male rats. At the same time, for the periods of four and six weeks, no influence on TBARS and protein carbonyl groups level was observed (p > 0.05), which may allow to suppose an adaptive mechanism of the growing organism while the ethanol and polyphenols exposition is prolonged. Further studies are required to confirm the mechanism of the pro-oxidative effect of polyphenols contained in red wine for the liver of growing organism.

Sign in / Sign up

Export Citation Format

Share Document