scholarly journals The essential role of Glu-185 and Tyr-354 residues in the ferroxidase activity of Saccharomyces cerevisiae Fet3

FEBS Letters ◽  
2000 ◽  
Vol 472 (2-3) ◽  
pp. 283-286 ◽  
Author(s):  
Maria Carmela Bonaccorsi di Patti ◽  
Maria Rosa Felice ◽  
Angela Pia Camuti ◽  
Amalia Lania ◽  
Giovanni Musci
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Essential Role ◽  
Ferroxidase Activity

scholarly journals An Overview on Saccharomyces cerevisiae Indigenous Strains Selection Methods

2021 ◽  
Author(s):  
Laura Pulcini ◽  
Elisa Gamalero ◽  
Antonella Costantini ◽  
Enrico Tommaso Vaudano ◽  
Christos Tsolakis ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Essential Role ◽  
Genomic Analysis ◽  
Point Of View ◽  
Market Demand ◽  
Wine Sector ◽  
Modern Vision ◽  
Xix Century ◽  
Natural Expression

From the fundamental studies of Louis Pasteur in the XIX century to the current genomic analysis, the essential role of microorganisms in winemaking industry is well recognised. In the last decades, selected Saccharomyces cerevisiae strains with excellent fermentative behaviour have been widely commercialised in form of active dry yeasts. Currently, the production of organic and “natural” wines represents a new economically relevant trend in the wine sector. Based on this market demand, the use of industrial yeast starter could be perceived as non-organic practice and then, rejected. However, in order to preserve wines sensory quality, healthiness, and to avoid organoleptic defects given by undesirable microorganisms, the “yeast factor” (S. cerevisiae or non-Saccharomyces) cannot be ignored. The purpose of this chapter is to describe the methods of selection of wine yeasts focusing the attention on indigenous S. cerevisiae strains. In fact, the use of ecotypic yeasts may represent a good compromise between the needs of microbiologically controlled fermentation and a modern vision of wine as natural expression of its “terroir”, also from the microbiological point of view.

scholarly journals Genome-scale analyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein degradation

2013 ◽  
Vol 6 (1) ◽  
pp. 48 ◽  
Author(s):  
Daniel González-Ramos ◽  
Marcel van den Broek ◽  
Antonius JA van Maris ◽  
Jack T Pronk ◽  
Jean-Marc G Daran
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Degradation ◽  
Essential Role ◽  
Butanol Tolerance ◽  
Genome Scale

scholarly journals Mig1 localization exhibits biphasic behavior which is controlled by both metabolic and regulatory roles of the sugar kinases

2020 ◽  
Vol 295 (6) ◽  
pp. 1489-1500
Author(s):  
Gregor W. Schmidt ◽  
Niek Welkenhuysen ◽  
Tian Ye ◽  
Marija Cvijovic ◽  
Stefan Hohmann
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Localization ◽  
Essential Role ◽  
Carbon Sources ◽  
Transcriptional Repressor ◽  
Energy Sources ◽  
Nucleocytoplasmic Shuttling

Abstract Glucose, fructose and mannose are the preferred carbon/energy sources for the yeast Saccharomyces cerevisiae. Absence of preferred energy sources activates glucose derepression, which is regulated by the kinase Snf1. Snf1 phosphorylates the transcriptional repressor Mig1, which results in its exit from the nucleus and subsequent derepression of genes. In contrast, Snf1 is inactive when preferred carbon sources are available, which leads to dephosphorylation of Mig1 and its translocation to the nucleus where Mig1 acts as a transcription repressor. Here we revisit the role of the three hexose kinases, Hxk1, Hxk2 and Glk1, in glucose de/repression. We demonstrate that all three sugar kinases initially affect Mig1 nuclear localization upon addition of glucose, fructose and mannose. This initial import of Mig1 into the nucleus was temporary; for continuous nucleocytoplasmic shuttling of Mig1, Hxk2 is required in the presence of glucose and mannose and in the presence of fructose Hxk2 or Hxk1 is required. Our data suggest that Mig1 import following exposure to preferred energy sources is controlled via two different pathways, where (1) the initial import is regulated by signals derived from metabolism and (2) continuous shuttling is regulated by the Hxk2 and Hxk1 proteins. Mig1 nucleocytoplasmic shuttling appears to be important for the maintenance of the repressed state in which Hxk1/2 seems to play an essential role.

scholarly journals Transcriptomic and chemogenomic analyses unveil the essential role of Com2-regulon in response and tolerance of Saccharomyces cerevisiae to stress induced by sulfur dioxide

2019 ◽  
Vol 6 (11) ◽  
pp. 509-523 ◽  
Author(s):  
Patrícia Lage ◽  
Belém Sampaio-Marques ◽  
Paula Ludovico ◽  
Nuno P. Mira ◽  
Ana Mendes-Ferreira
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sulfur Dioxide ◽  
Essential Role

Essential role of Sharpin in TNFα dependent NFκB activation in primary hepatocytes

2012 ◽  
Vol 50 (01) ◽  
Author(s):  
N Lange ◽  
S Sieber ◽  
A Erhardt ◽  
G Sass ◽  
HJ Kreienkamp ◽  
...  
Keyword(s):  
Essential Role ◽  
Primary Hepatocytes

Different Abilities of Thrombin Receptor Activating Peptide and Thrombin to Induce Platelet Calcium Rise and Full Release Reaction

1995 ◽  
Vol 74 (05) ◽  
pp. 1323-1328 ◽  
Author(s):  
Dominique Lasne ◽  
José Donato ◽  
Hervé Falet ◽  
Francine Rendu
Keyword(s):  
Essential Role ◽  
Calcium Influx ◽  
Dense Granule ◽  
Receptor Interaction ◽  
Thrombin Receptor ◽  
Release Reaction ◽  
External Calcium ◽  
Maximum Rise ◽  
Granule Release

SummarySynthetic peptides (TRAP or Thrombin Receptor Activating Peptide) corresponding to at least the first five aminoacids of the new N-terminal tail generated after thrombin proteolysis of its receptor are effective to mimic thrombin. We have studied two different TRAPs (SFLLR, and SFLLRN) in their effectiveness to induce the different platelet responses in comparison with thrombin. Using Indo-1/AM- labelled platelets, the maximum rise in cytoplasmic ionized calcium was lower with TRAPs than with thrombin. At threshold concentrations allowing maximal aggregation (50 μM SFLLR, 5 μM SFLLRN and 1 nM thrombin) the TRAPs-induced release reaction was about the same level as with thrombin, except when external calcium was removed by addition of 1 mM EDTA. In these conditions, the dense granule release induced by TRAPs was reduced by over 60%, that of lysosome release by 75%, compared to only 15% of reduction in the presence of thrombin. Thus calcium influx was more important for TRAPs-induced release than for thrombin-induced release. At strong concentrations giving maximal aggregation and release in the absence of secondary mediators (by pretreatment with ADP scavengers plus aspirin), SFLLRN mobilized less calcium, with a fast return towards the basal level and induced smaller lysosome release than did thrombin. The results further demonstrate the essential role of external calcium in triggering sustained and full platelet responses, and emphasize the major difference between TRAP and thrombin in mobilizing [Ca2+]j. Thus, apart from the proteolysis of the seven transmembrane receptor, another thrombin binding site or thrombin receptor interaction is required to obtain full and complete responses.

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