Induction of ras gene expression by homologous aggregation factor in cells from the sponge Geodia cydonium.

AbstractRas gene family members play a relevant role in cancer, especially when they are mutated. However, they may play additional roles in other conditions beside cancer. We performed gene expression analysis using the NanoString PanCancer IO 360 panel in the peripheral blood mononuclear cell (PBMC) of six COVID-19 patients and we found that H-Ras gene was significantly upregulated, while both K-Ras and N-Ras genes were downregulated. In particular, H-Ras gene upregulation was more evident in COVID-19 patients with a more severe disease. We compared our results with those obtained by analyzing two different and independent datasets, including a total of 53 COVID-19 patients, in which the gene expression analysis was performed using the Immunology_V2 panel. Comparative analysis of the H-Ras gene expression in these patients confirmed our preliminary results. In both of them, in fact, we were able to confirm the upregulation of the expression of the H-Ras gene. The exact role of this specific upregulation of the H-Ras gene in response to SARS-CoV-2 infection and its possible role in cancer still remains to be elucidated. In conclusion, H-Ras gene participates to the host immune response to SARS-CoV-2 virus infection, especially in patients affected by the most severe form of the COVID-19.

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Toll-like receptor 2 induced senescence in intervertebral disc cells of patients with back pain can be attenuated by o-vanillin

Arthritis Research & Therapy ◽

10.1186/s13075-021-02504-z ◽

2021 ◽

Vol 23 (1) ◽

Author(s):

Matthew Mannarino ◽

Hosni Cherif ◽

Li Li ◽

Kai Sheng ◽

Oded Rabau ◽

...

Keyword(s):

Gene Expression ◽

Back Pain ◽

Protein Expression ◽

Disc Degeneration ◽

Cell Senescence ◽

Enzyme Linked Immunosorbent Assay ◽

Matrix Synthesis ◽

Gene And Protein Expression ◽

Pain Patients ◽

In Cells

Abstract Background There is an increased level of senescent cells and toll-like teceptor-1, -2, -4, and -6 (TLR) expression in degenerating intervertebral discs (IVDs) from back pain patients. However, it is currently not known if the increase in expression of TLRs is related to the senescent cells or if it is a more general increase on all cells. It is also not known if TLR activation in IVD cells will induce cell senescence. Methods Cells from non-degenerate human IVD were obtained from spine donors and cells from degenerate IVDs came from patients undergoing surgery for low back pain. Gene expression of TLR-1,2,4,6, senescence and senescence-associated secretory phenotype (SASP) markers was evaluated by RT-qPCR in isolated cells. Matrix synthesis was verified with safranin-O staining and Dimethyl-Methylene Blue Assay (DMMB) confirmed proteoglycan content. Protein expression of p16INK4a, SASP factors, and TLR-2 was evaluated by immunocytochemistry (ICC) and/or by enzyme-linked immunosorbent assay (ELISA). Results An increase in senescent cells was found following 48-h induction with a TLR-2/6 agonist in cells from both non-degenerate and degenerating human IVDs. Higher levels of SASP factors, TLR-2 gene expression, and protein expression were found following 48-h induction with TLR-2/6 agonist. Treatment with o-vanillin reduced the number of senescent cells, and increased matrix synthesis in IVD cells from back pain patients. Treatment with o-vanillin after induction with TLR-2/6 agonist reduced gene and protein expression of SASP factors and TLR-2. Co-localized staining of p16INK4a and TLR-2 demonstrated that senescent cells have a high TLR-2 expression. Conclusions Taken together our data demonstrate that activation of TLR-2/6 induce senescence and increase TLR-2 and SASP expression in cells from non-degenerate IVDs of organ donors without degeneration and back pain and in cells from degenerating human IVD of patients with disc degeneration and back pain. The senescent cells showed high TLR-2 expression suggesting a link between TLR activation and cell senescence in human IVD cells. The reduction in senescence, SASP, and TLR-2 expression suggest o-vanillin as a potential disease-modifying drug for patients with disc degeneration and back pain.

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DMBA Induction Increases H-Ras Gene Expression and Decreases CD8 Count in Sprague Dawley Rats

Proceedings of the 2019 6th International Conference on Biomedical and Bioinformatics Engineering ◽

10.1145/3375923.3375942 ◽

2019 ◽

Author(s):

Titiek Hidayati ◽

Indrayanti ◽

Sagiran

Keyword(s):

Gene Expression ◽

Sprague Dawley ◽

Ras Gene ◽

Sprague Dawley Rats

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HIV-1 Tat Elongates the G1Phase and Indirectly Promotes HIV-1 Gene Expression in Cells of Glial Origin

Journal of Biological Chemistry ◽

10.1074/jbc.273.14.8130 ◽

1998 ◽

Vol 273 (14) ◽

pp. 8130-8136 ◽

Cited By ~ 24

Author(s):

Mondira Kundu ◽

Sunita Sharma ◽

Antonio De Luca ◽

Antonio Giordano ◽

Jay Rappaport ◽

...

Keyword(s):

Gene Expression ◽

Hiv 1 ◽

In Cells

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Corrigendum to “Radiation response and regulation of apoptosis induced by a combination of TRAIL and CHX in cells lacking mitochondrial DNA: A role for NF-κB–STAT3-directed gene expression” [Exp. Cell Res. 317 (2011) 1548–1566]

Experimental Cell Research ◽

10.1016/j.yexcr.2021.112776 ◽

2021 ◽

pp. 112776

Author(s):

Vladimir N. Ivanov ◽

Shanaz A. Ghandhi ◽

Hongning Zhou ◽

Sarah X. Huang ◽

Yunfei Chai ◽

...

Keyword(s):

Gene Expression ◽

Mitochondrial Dna ◽

Radiation Response ◽

In Cells

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Identification of a regulatory region that mediates glucose-dependent induction of the Saccharomyces cerevisiae enolase gene ENO2

Molecular and Cellular Biology ◽

10.1128/mcb.6.7.2287-2297.1986 ◽

1986 ◽

Vol 6 (7) ◽

pp. 2287-2297

Author(s):

R Cohen ◽

J P Holland ◽

T Yokoi ◽

M J Holland

Keyword(s):

Gene Expression ◽

Regulatory Region ◽

Carbon Sources ◽

Initiation Site ◽

Deletion Mapping ◽

Regulatory Regions ◽

Coding Sequences ◽

Flanking Region ◽

Fused Gene ◽

In Cells

There are two yeast enolase genes, designated ENO1 and ENO2, which are expressed differentially in vegetative cells grown on glucose and in cells grown on gluconeogenic carbon sources. ENO2 is induced more than 20-fold in cells grown on glucose, whereas ENO1 expression is similar in cells grown on glucose and in cells grown on gluconeogenic carbon sources. Sequences within the 5' flanking region of ENO2 which are required for glucose-dependent induction were identified by deletion mapping analysis. These studies were carried out by using a fused gene containing the ENO2 5' flanking sequences and the ENO1 coding sequences. This fused gene undergoes glucose-dependent induction and is expressed at the same level as the resident ENO2 gene in cells grown on glucose or gluconeogenic carbon sources. Expression of fused genes containing deletion mutations within the ENO2 5' flanking region was monitored after integration at the ENO1 locus of a strain carrying a deletion of the resident ENO1 coding sequences. This analysis showed that there are two upstream activation sites located immediately upstream and downstream from a position 461 base pairs upstream from the transcriptional initiation site. Either one of these upstream activation sites is sufficient for glucose-dependent induction and normal gene expression in the presence of gluconeogenic carbon sources. Deletion of both regulatory regions results in a complete loss of gene expression. The regulatory regions function normally in both orientations relative to the coding sequences. Mutant fused genes containing small deletions within the regulatory regions were constructed; these genes were expressed normally in gluconeogenic carbon sources but were not induced in the presence of glucose. Based on this analysis, ENO2 contains a cis-acting regulatory region which is required for gene expression and mediates glucose-dependent induction of gene expression.

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