Influence of Baseline Oxidative Stress Level on Changes in Left Ventricle Echocardiographic Parameters in Patients with Breast Cancer Receiving Anthracycline Chemotherapy

Objective This study was carried out to investigate interval exercise on Smyd1 expression and F-actin sarcomere assembly in non-infarcted myocardium of normal and myocardial infarction(MI) rats and its possible mechanism. Methods Male SD rats were randomly divided into normal control group (C), normal interval exercise group (CE), sham-operated group (S), MI group (MI), MI with interval exercise group (ME) and MI with ROS Tempol group (MT), n=10. MI model was established by left anterior descending coronary artery ligation. Interval exercise was carried out on a small animal treadmill. MT group was given an oral solution of Tempol (2mmol/L). Hemodynamics was performed to evaluate cardiac function. HE and Masson staining were used to analyze the cross-sectional area (CSA) of cardiomyocytes and collagen volume fraction, respectively. T-SOD and MDA kits were used to detect oxidative stress. H9C2 cells were treated with H2O2. Immunofluorescence staining was used to determine Smyd1 expression and F-actin sarcomere assembly. RT-qPCR and Western blotting were used to detect the gene or protein expression of Smyd1, Trx1, Hsp90, MuRF1, cTnI, α-actinin and BNP. Results Smyd1, Trx1, Hsp90, MuRF1 and BNP expression in the peri-infarcted area were up-regulated, but cTnI and α-actinin expression and F-actin assembly were decreased. The cardiac function was reduced. Both interval exercise and Tempol intervention significantly increase the CSA and expression of Smyd1, Trx1, cTnI and α-actinin, improve the antioxidation capacity and F-actin sarcomere assembly and cardiac function, reduce the expression of Hsp90, MuRF1, BNP and ROS level, and inhibit the fibrosis of myocardium. The oxidative stress level was closely related to the Smyd1 expression. Improvement of cardiac function were correlated with Smyd1 expression. H2O2 can induce oxidative stress injuries of H9C2, and its closely related to cardiomyocytes oxidative stress level and Smyd1 expression. Conclusions Interval exercise could promote antioxidant capability and physiological cardiomyocyte hypertrophy, regulate the expression of Smyd1, Hsp90 and MuRF1 in infarcted heart; so as to improve the cardiac function. Smyd1 may participate in pathologic hypertrophy of cardiomyocytes caused by oxidative stress.

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Asprosin and Oxidative Stress Level in COVID-19 Patients

Clinical Laboratory ◽

10.7754/clin.lab.2021.210423 ◽

2022 ◽

Vol 68 (01/2022) ◽

Author(s):

E. Seyhanli ◽

Ismail Koyuncu ◽

I. Yasak ◽

H. Demir ◽

Ebru Temiz

Keyword(s):

Oxidative Stress ◽

Stress Level ◽

Oxidative Stress Level ◽

And Oxidative Stress

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Empathy and Oxidative Stress in Healthy Adults

Sustainability ◽

10.3390/su12124959 ◽

2020 ◽

Vol 12 (12) ◽

pp. 4959 ◽

Cited By ~ 1

Author(s):

Agnieszka Żelaźniewicz ◽

Judyta Nowak ◽

Agata Groyecka ◽

Piotr Sorokowski ◽

Małgorzata Dobrowolska ◽

...

Keyword(s):

Oxidative Stress ◽

Stress Level ◽

Oxidative Dna Damage ◽

Behavioral Changes ◽

Social Environments ◽

Self Assessment ◽

Adverse Conditions ◽

Oxidative Stress Level ◽

Empathy Quotient ◽

Study Participants

Empathy is crucial for normal and effective social functioning, enabling comprehension and prediction of actions in social environments. Despite its importance for maintaining social relationships in human groups, the physiological correlates of empathy are not fully known. The aim of this study was to test whether empathy is related to oxidative stress level, that may result both from internal disturbances and influence of external adverse factors. Seventy-four healthy women (Mage = 26.23, SDage = 2.88) and one hundred and one men (Mage = 28.09, SDage = 3.03) took part in the study. Participants’ empathy was evaluated with self-assessment questionnaire—Empathy Quotient (EQ). Oxidative stress level was measured with serum 8-OH-dG, a product of oxidative DNA damage. The results showed that empathy is negatively related to oxidative stress level in men but not in women, when controlled for testosterone level. Revealed sex differences may be explained by men’s greater vulnerability to various adverse conditions and harmful factors. Men, compared to women, seem to be more susceptible to behavioral changes, induced by increased oxidative stress level. The study adds to growing evidence showing that many physiological mechanisms, other than hormonal factors, that may be also related with environmental harmful factors, are related to behavioral, affective and cognitive phenomena.

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Circulating Leukocytes and Oxidative Stress in Cardiovascular Diseases: A State of the Art

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/2650429 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Speranza Rubattu ◽

Maurizio Forte ◽

Salvatore Raffa

Keyword(s):

Oxidative Stress ◽

Cardiovascular Diseases ◽

Stress Level ◽

Mononuclear Cells ◽

Pathological Condition ◽

Clinical Manifestations ◽

Therapeutic Interventions ◽

Oxidative Stress Level ◽

The Impact

Increased oxidative stress from both mitochondrial and cytosolic sources contributes to the development and the progression of cardiovascular diseases (CVDs), and it is a target of therapeutic interventions. The numerous efforts made over the last decades in order to develop tools able to monitor the oxidative stress level in patients affected by CVDs rely on the need to gain information on the disease state. However, this goal has not been satisfactorily accomplished until now. Among others, the isolation of circulating leukocytes to measure their oxidant level offers a valid, noninvasive challenge that has been tested in few pathological contexts, including hypertension, atherosclerosis and its clinical manifestations, and heart failure. Since leukocytes circulate in the blood stream, it is expected that they might reflect quite closely both systemic and cardiovascular oxidative stress and provide useful information on the pathological condition. The results of the studies discussed in the present review article are promising. They highlight the importance of measuring oxidative stress level in circulating mononuclear cells in different CVDs with a consistent correlation between degree of oxidative stress and severity of CVD and of its complications. Importantly, they also point to a double role of leukocytes, both as a marker of disease condition and as a direct contributor to disease progression. Finally, they show that the oxidative stress level of leukocytes reflects the impact of therapeutic interventions. It is likely that the isolation of leukocytes and the measurement of oxidative stress, once adequately developed, may represent an eligible tool for both research and clinical purposes to monitor the role of oxidative stress on the promotion and progression of CVDs, as well as the impact of therapies.

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Expression of FOXO6 is Associated With Oxidative Stress Level and Predicts the Prognosis in Hepatocellular Cancer

Medicine ◽

10.1097/md.0000000000003708 ◽

2016 ◽

Vol 95 (21) ◽

pp. e3708 ◽

Cited By ~ 8

Author(s):

Hai-Yong Chen ◽

Yao-Min Chen ◽

Jian Wu ◽

Fu-Chun Yang ◽

Zhen Lv ◽

...

Keyword(s):

Oxidative Stress ◽

Stress Level ◽

Hepatocellular Cancer ◽

Oxidative Stress Level

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Anthracycline-Induced Cardiotoxicity in Breast Cancer Patients from Southern Sri Lanka: An Echocardiographic Analysis

BioMed Research International ◽

10.1155/2020/1847159 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Jayasinghe Arachchige Nirosha Sandamali ◽

Ruwani Punyakanthi Hewawasam ◽

Madappuli Arachchige Chaminda Sri Sampath Fernando ◽

Kamani Ayoma Perera Wijewardana Jayatilaka ◽

Ranji Duleep Madurawe ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Patients ◽

Left Ventricular ◽

Breast Cancer Patients ◽

Anthracycline Therapy ◽

Echocardiographic Parameters ◽

Chemotherapy Dose ◽

Anthracycline Dose ◽

Anthracycline Chemotherapy

Anthracycline-induced cardiotoxicity has never been investigated in Sri Lanka. Therefore, this study was conducted to determine the prevalence of anthracycline-induced cardiotoxicity in breast cancer patients using echocardiographic findings. A prospective cohort study was performed. All newly diagnosed breast cancer patients who were administered with anthracycline and cyclophosphamide (AC schedule) for the first time were enrolled in the study. In the hospital setting, anthracycline is administered only as a combination therapy, and only this combination was selected to limit the effect of other cardiotoxic chemotherapy agents. Records of echocardiography were obtained: one day before anthracycline chemotherapy (baseline), one day after the first chemotherapy dose, one day after the last chemotherapy dose, and six months after the completion of anthracycline chemotherapy. Following parameters were recorded from the echocardiography results: ejection fraction (EF, %), fractioning shortening (FS, %), posterior wall thickness, left ventricle (PWT, mm), the thickness of interventricular septum (IVS, mm), left ventricular end-diastolic diameter (LVEDD, mm), and left ventricular end-systolic diameter (LVESD, mm). Statistical analysis of the echocardiography results was performed using ANOVA at four stages. A p value <0.05 was considered significant. Subclinical cardiac dysfunction was defined as a fall of EF >10% during the follow-up echocardiography. There was no significant change ( p > 0.05 ) between the baseline echocardiographic parameters and one day after the 1st anthracycline dose. However, significant differences ( p < 0.05 ) were observed between the baseline echocardiographic parameters and one day after the last anthracycline dose and six months after the completion of anthracycline therapy with a gradual and progressive deterioration in functional parameters including EF, FS, PWT, and IVS over time. There were 65 patients out of 196 (33.16%) who developed subclinical cardiac dysfunction six months after the completion of anthracycline chemotherapy. The prevalence of subclinical anthracycline-induced cardiotoxicity was relatively higher in these patients. An equation was also developed based on left ventricular ejection fraction (LVEF) to predict the anthracycline-induced cardiotoxicity of a patient six months after the completion of anthracycline chemotherapy. We believe that this will help in the monitoring of patients who undergo anthracycline therapy for cardiotoxicity. It is recommended to carry out a long-term follow-up to detect early-onset chronic progressive cardiotoxicity in all patients who were treated with anthracycline therapy.

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Oxidative stress, plasma/salivary antioxidant status detection and health risk factors

Asian Journal of Medical Sciences ◽

10.3126/ajms.v8i1.15134 ◽

2017 ◽

Vol 8 (1) ◽

pp. 32-41 ◽

Cited By ~ 1

Author(s):

Vadala Maria ◽

Palmieri Beniamino ◽

Malagoli Andrea ◽

Laurino Carmen

Keyword(s):

Oxidative Stress ◽

Risk Factors ◽

Oral Cavity ◽

Health Risk ◽

Stress Level ◽

Antioxidant Status ◽

Hormone Replacement ◽

Smoking Habit ◽

Health Risk Factors ◽

Oxidative Stress Level

Background: Oxidative stress is involved in the pathophysiology of several diseases such as cardiovascular disorders, cancer, neurodegeneration.Aims and Objectives: Our study evaluated the oxidant/antioxidant status on a cohort of healthy patients matched with some independent variables as a basic individual redox balance monitor on a disease-prevention perspective.Materials and Methods: The anecdotic, retrospective and observational study included 200 apparently healthy volunteers after formal informed consent release whose personal history and physical examination had been recorded specifically on the following items: age, previous diseases, sport activities, smoking habit, balanced/unbalanced nutrition, current absence/presence of inflammatory processes, oral health hygiene, administration of oral contraceptives or hormone replacement therapy in postmenopausal women. No drug treatment was admitted in the recruited patients, up to 6 months before the evaluation. The laboratory instruments used were Point of care FRAS 4 Evolvo (H&D, Parma, Italy) measuring the oxidative stress in plasma samples and antioxidant capacity in plasma and saliva samples.Results: Two-hundred patients were recruited. Statistically relevant differences were observed in oxidative stress-related variables, namely a significant relationship between plasma oxidative stress level and female gender (p<0.01), between saliva antioxidant level and age (p=0.01), between plasma antioxidant level and unbalanced diet (p<0.01), between plasma oxidative stress level and inflammation in the oral cavity (p=0.04), and between saliva antioxidant level and inflammation in the oral cavity (p<0.01).Conclusions: A relationship between oxidative/antioxidant status and health risk factors has been outlined in our study; the achieved data are quite helpful, in the clinical practice, providing additional information on individual general health conditions, putatively related to prevention diagnosis, prognosis, and treatment effectiveness in some specific diseases.Asian Journal of Medical Sciences Vol.8(1) 2017 32-41

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