P010 The effect of combination treatment of activated NK cell with radiation therapy on bladder cancer cell

2013 ◽  
Vol 12 (6) ◽  
pp. 134
Author(s):  
T.W. Kang ◽  
H. Park ◽  
S. Kwon ◽  
J.S. Kim ◽  
Y.R. Kim ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Radiation Therapy ◽  
Cancer Cell ◽  
Combination Treatment ◽  
Nk Cell ◽  
Bladder Cancer Cell

scholarly journals Dual Targeting of FGFR3 and ERBB3 Enhances The Efficacy of FGFR Inhibitors in FGFR3 Fusion-Driven Bladder Cancer

2021 ◽  
Author(s):  
Andrew J Weickhardt ◽  
David K Lau ◽  
Margeaux Hodgson-Garms ◽  
Austen Lavis ◽  
Laura J Jenkins ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Combination Treatment ◽  
Acquired Resistance ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Long Term Treatment ◽  
Inhibitor Treatment

Abstract Background Mutations and fusions in Fibroblast Growth Factor Receptor 3 (FGFR3) occur in 10-20% of metastatic urothelial carcinomas and confers sensitivity to FGFR inhibitors. However, responses to these agents are often short-lived due to the development of acquired resistance. The objective of this study was to identify mechanisms of resistance to FGFR inhibitors in two previously uncharacterised bladder cancer cell lines harbouring FGFR3 fusions and assess rational combination therapies to enhance sensitivity to these agents. Methods Acquired resistance to FGFR inhibitors was generated in two FGFR3 fusion harbouring cell lines, SW780 (FGFR3-BAIAP2L1 fusion) and RT4 (FGFR3-TACC3 fusion), by long-term exposure to the FGFR inhibitor BGJ398. Changes in levels of receptor tyrosine kinases were assessed by phospho-RTK arrays and immunoblotting. Changes in cell viability and proliferation were assessed by the Cell-Titre Glo assay and by propidium iodide staining and FACS analysis. Results Long term treatment of FGFR3-fusion harbouring SW780 and RT4 bladder cancer cell lines with the BGJ398 resulted in the establishment of resistant clones. These clones were cross-resistant to the clinically approved FGFR inhibitor erdafitinib and the covalently binding irreversible FGFR inhibitor TAS-120, but remained sensitive to the MEK inhibitor trametinib, indicating resistance is mediated by alternate activation of MAPK signalling. The FGFR inhibitor-resistant SW780 and RT4 lines displayed increased expression of pERBB3, and strikingly, combination treatment with an FGFR inhibitor and the ATP-competitive pan-ERBB inhibitor AZD8931 overcame this resistance. Notably, rapid induction of pERBB3 and reactivation of pERK also occurred in parental FGFR3 fusion-driven lines within 24 hours of FGFR inhibitor treatment, and combination treatment with an FGFR inhibitor and AZD8931 delayed the reactivation of pERBB3 and pERK and synergistically inhibited cell proliferation. Conclusions We demonstrate that increased expression of pERBB3 is a key mechanism of adaptive resistance to FGFR inhibitors in FGFR3-fusion driven bladder cancers, and that this also occurs rapidly following FGFR inhibitor treatment. Our findings demonstrate that resistance can be overcome by combination treatment with a pan-ERBB inhibitor and suggest that upfront combination treatment with FGFR and pan-ERBB inhibitors warrants further investigation for FGFR3-fusion harbouring bladder cancers.

624: Usefulness of Cancer Vaccine Therapy Using Bladder Cancer Cell Line Introduced with Interleukin 21 Gene

2006 ◽  
Vol 175 (4S) ◽  
pp. 201-201 ◽  
Author(s):  
Isao Hara ◽  
Junya Furukawa ◽  
Kazuki Yamanaka ◽  
Yuji Yamada ◽  
Masato Fujisawa
Keyword(s):  
Bladder Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Vaccine ◽  
Cancer Cell Line ◽  
Bladder Cancer Cell ◽  
Vaccine Therapy ◽  
Bladder Cancer Cell Line ◽  
Interleukin 21

CHEMOSENSITIZATION OF BLADDER CANCER CELL LINES BY HUMAN TELOMERASE REVERSE TRANSCRIPTASE ANTISENSE TREATMENT

2004 ◽  
Vol 172 (5) ◽  
pp. 2023-2028 ◽  
Author(s):  
KAI KRAEMER ◽  
SUSANNE FUESSEL ◽  
MATTHIAS KOTZSCH ◽  
SHUANGLI NING ◽  
UTA SCHMIDT ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Reverse Transcriptase ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Human Telomerase

scholarly journals Protein expression profile related to cisplatin resistance in bladder cancer cell lines detected by two-dimensional gel electrophoresis

2015 ◽  
Vol 36 (4) ◽  
pp. 253-261 ◽  
Author(s):  
Yoshinori TAOKA ◽  
Kazumasa MATSUMOTO ◽  
Kazuya OHASHI ◽  
Satoru MINAMIDA ◽  
Masahiro HAGIWARA ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Protein Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Expression Profile ◽  
Cisplatin Resistance ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Two Dimensional ◽  
Protein Expression Profile

Expression of glutathione-related enzymes in human bladder cancer cell lines

1990 ◽  
Vol 39 (11) ◽  
pp. 1817-1820 ◽  
Author(s):  
Shivendra V. Singh ◽  
Hassan Ahmad ◽  
Awtar Krishan
Keyword(s):  
Bladder Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Human Bladder Cancer Cell
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