P068 High expression level of the T cell potassium channel, KV1.3, is a novel diagnostic marker of inflammatory activity in patients with ulcerative colitis

Lung cancer is the most common malignant tumor, accounting for 25% of cancer-related deaths and 14% of new cancers worldwide. Lung adenocarcinoma is the most common type of pulmonary cancer. Although there have been some improvements in the traditional therapy of lung cancer, the outcome and prognosis of patients remain poor. Lung cancer is the leading cause of cancer-related deaths worldwide, with 1.8 million new cases being diagnosed each year. Precision medicine based on genetic alterations is considered a new strategy of lung cancer treatment that requires highly specific biomarkers for precision diagnosis and treatment. Fibrinogen-like protein 2 (FGL2) plays important roles in both innate and adaptive immunity. However, the diagnostic value of FGL2 in lung cancer is largely unknown. In this study, we systematically investigated the expression profile and potential functions of FGL2 in lung adenocarcinoma. We used the TCGA and Oncomine datasets to compare the FGL2 expression levels between lung adenocarcinoma and adjacent normal tissues. We utilized the GEPIA, PrognoScan and Kaplan-Meier plotter databases to analyze the relationship between FGL2 expression and the survival of lung adenocarcinoma patients. Then, we investigated the potential roles of FGL2 in lung adenocarcinoma with the TIMER database and functional enrichment analyses. We found that FGL2 expression was significantly lower in lung adenocarcinoma tissue compared with adjacent normal tissue. A high expression level of FGL2 was correlated with better prognostic outcomes of lung adenocarcinoma patients, including overall survival and progression-free survival. FGL2 was positively correlated with the infiltration of immune cells, including dendritic cells, CD8+ T cells, macrophages, B cells, and CD4+ T cells, in lung adenocarcinoma. Functional enrichment analyses also showed that a high expression level of FGL2 was positively correlated with enhanced T cell activities, especially CD8+ T cell activation. Thus, we propose that high FGL2 expression, which is positively associated with enhanced antitumor activities mediated by T cells, is a beneficial marker for lung adenocarcinoma treatment outcomes.

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CD56+ T-cell Prolymphocytic Leukemia Showing a High Expression Level ofTCL1Oncogene: A Case Report with a Review of the Literature

The Korean Journal of Hematology ◽

10.5045/kjh.2006.41.2.129 ◽

2006 ◽

Vol 41 (2) ◽

pp. 129

Author(s):

Hyeong-Kee Yun ◽

Myung-Geun Shin ◽

Hyeoung-Joon Kim ◽

Hye-Ran Kim ◽

Ji-Young Park ◽

...

Keyword(s):

Case Report ◽

T Cell ◽

High Expression ◽

Expression Level ◽

Review Of The Literature ◽

Prolymphocytic Leukemia ◽

High Expression Level

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High expression level of T-box transcription factor 5 predicts unfavorable survival in stage I and II gastric adenocarcinoma

Oncology Letters ◽

10.3892/ol.2015.3515 ◽

2015 ◽

Vol 10 (4) ◽

pp. 2021-2026 ◽

Cited By ~ 3

Author(s):

YAN ZHENG ◽

YUAN-FANG LI ◽

WEI WANG ◽

YONG-MING CHEN ◽

DAN-DAN WANG ◽

...

Keyword(s):

Transcription Factor ◽

Gastric Adenocarcinoma ◽

Stage I ◽

High Expression ◽

Expression Level ◽

T Box ◽

High Expression Level

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High expression level of geminin predicts a poor clinical outcome in salivary gland carcinomas

Histopathology ◽

10.1111/j.1365-2559.2010.03561.x ◽

2010 ◽

Vol 56 (7) ◽

pp. 883-892 ◽

Cited By ~ 13

Author(s):

Manabu Yamazaki ◽

Satoshi Fujii ◽

Yukinori Murata ◽

Ryuichi Hayashi ◽

Atsushi Ochiai

Keyword(s):

Salivary Gland ◽

Clinical Outcome ◽

High Expression ◽

Expression Level ◽

Poor Clinical Outcome ◽

Salivary Gland Carcinomas ◽

High Expression Level

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The immune-suppressive landscape in lepromatous leprosy revealed by single-cell RNA sequencing

Cell Discovery ◽

10.1038/s41421-021-00353-3 ◽

2022 ◽

Vol 8 (1) ◽

Author(s):

Zihao Mi ◽

Zhenzhen Wang ◽

Xiaotong Xue ◽

Tingting Liu ◽

Chuan Wang ◽

...

Keyword(s):

T Cells ◽

Single Cell ◽

Rna Sequencing ◽

Lepromatous Leprosy ◽

High Expression ◽

Expression Level ◽

Intracellular Bacteria ◽

Monocyte Subset ◽

Single Cell Rna Sequencing ◽

High Expression Level

AbstractLepromatous leprosy (L-LEP), caused by the massive proliferation of Mycobacterium leprae primarily in macrophages, is an ideal disease model for investigating the molecular mechanism of intracellular bacteria evading or modulating host immune response. Here, we performed single-cell RNA sequencing of both skin biopsies and peripheral blood mononuclear cells (PBMCs) of L-LEP patients and healthy controls. In L-LEP lesions, we revealed remarkable upregulation of APOE expression that showed a negative correlation with the major histocompatibility complex II gene HLA-DQB2 and MIF, which encodes a pro-inflammatory and anti-microbial cytokine, in the subset of macrophages exhibiting a high expression level of LIPA. The exhaustion of CD8+ T cells featured by the high expression of TIGIT and LAG3 in L-LEP lesions was demonstrated. Moreover, remarkable enhancement of inhibitory immune receptors mediated crosstalk between skin immune cells was observed in L-LEP lesions. For PBMCs, a high expression level of APOE in the HLA-DRhighFBP1high monocyte subset and the expansion of regulatory T cells were found to be associated with L-LEP. These findings revealed the primary suppressive landscape in the L-LEP patients, providing potential targets for the intervention of intracellular bacteria caused persistent infections.

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Characterization and analysis of an industrial strain of Streptomyces bingchenggensis by genome sequencing and gene microarray

Genome ◽

10.1139/gen-2013-0098 ◽

2013 ◽

Vol 56 (11) ◽

pp. 677-689 ◽

Cited By ~ 7

Author(s):

Xiang-Jing Wang ◽

Bo Zhang ◽

Yi-Jun Yan ◽

Jing An ◽

Ji Zhang ◽

...

Keyword(s):

Natural Products ◽

Gene Cluster ◽

Crop Protection ◽

Gc Content ◽

Gene Clusters ◽

High Expression ◽

Expression Level ◽

Transcriptional Analysis ◽

Streptomyces Bingchenggensis ◽

High Expression Level

Streptomyces bingchenggensis is a soil bacterium that produces milbemycins, a family of macrolide antibiotics that are commercially important in crop protection and veterinary medicine. In addition, S. bingchenggensis produces many other natural products including the polyether nanchangmycin and novel cyclic pentapeptides. To identify the gene clusters involved in the biosynthesis of these compounds, and better clarify the biochemical pathways of these gene clusters, the whole genome of S. bingchenggensis was sequenced, and the transcriptome profile was subsequently investigated by microarray. In comparison with other sequenced genomes in Streptomyces, S. bingchenggensis has the largest linear chromosome consisting of 11 936 683 base pairs (bp), with an average GC content of 70.8%. The 10 023 predicted protein-coding sequences include at least 47 gene clusters correlated with the biosynthesis of known or predicted secondary metabolites. Transcriptional analysis demonstrated an extremely high expression level of the milbemycin gene cluster during the entire growth period and a moderately high expression level of the nanchangmycin gene cluster during the initial hours that subsequently decreased. However, other gene clusters appear to be silent. The genome-wide analysis of the secondary metabolite gene clusters in S. bingchenggensis, coupled with transcriptional analysis, will facilitate the rational development of high milbemycins-producing strains as well as the discovery of new natural products.

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