Effects of omitting lysine from diets conforming to Agricultural Research Council (1981) standards for pigs

1984 ◽  
Vol 39 (3) ◽  
pp. 449-453 ◽  
Author(s):  
M. F. Fuller ◽  
A. Cadenhead ◽  
K. Pennie

ABSTRACTEight diets based on barley and soya bean meal were formulated. Two basal diets, one of which included fish meal, were formulated to conform to the recommendations of the Agricultural Research Council (1981) for all amino acids except lysine. Four further diets were made by adding L-lysine to these diets, in two increments; the highest concentrations of lysine were also supplied in two further diets by the inclusion of a higher proportion of soya bean meal. Each diet was given ad libitum to 10 growing pigs, five castrated males and five females, which were housed and fed individually.Measurements of performance were made over the weight range 22 to 55 kg. Carcasses were appraised after slaughter at a mean weight of 75 kg.Addition of lysine, but not of soya bean meal, significantly increased daily food intake and carcass fatness. Daily growth and food conversion efficiency were not significantly affected. Because of the high food intake the daily lysine intakes on the basal diets greatly exceeded the suggested daily requirement and the results indicate that in such circumstances the response to the amino acid supplementation of an apparently inadequate protein may be negligible.

1988 ◽  
Vol 46 (2) ◽  
pp. 181-193 ◽  
Author(s):  
J. R. Newbold ◽  
P. C. Garnsworthy ◽  
P. J. Buttery ◽  
D. J. A. Cole ◽  
W. Haresign

AbstractThe ability of the protein nutrition scheme proposed by the Agricultural Research Council (ARC, 1980, 1984) to predict responses to protein supply was examined in two experiments. In experiment 1, groups of nine British Friesian steers implanted with oestradiol-17β and nine non-implanted steers were fed from 133 to 300 kg live weight on each of four all-concentrate diets (metabolizable energy (ME) = 12 MJ/kg dry matter (DM)) containing ratios of soya-bean meal and formaldehyde-treated soya-bean meal such that undegradable protein (UDP) concentration was 19, 25, 32 or 42 g/kg DM. Rumen degradable protein (RDP) concentration was relatively constant (111 to 116 g/kg DM). Implantation did not affect DM intake (DMI, g/kg M0·75). Both live-weight gain (LWG) and food conversion efficiency (FCE) (LWG/DMI) were greater (P < 0·05) for the implanted cattle (LWG = 1·41 (s.e. 0·04) kg/day; FCE = 0·24 (s.e. 0·02)) than for the non-implanted cattle (LWG = 1·23 (s.e. 0·05) kg/day; FCE = 0·22 (s.e. 0·02)). There were no dietary effects on either DMI or FCE. In the non-implanted steers, UDP did not affect LWG but, for the implanted steers, there were positive, linear responses in LWG to both UDP concentration (P = 0·048) and UDP intake (P = 0·026). In experiment 2, groups of eight implanted steers were fed from 132 to 300 kg live weight on each of six diets (ME = 12 MJ/kg DM, soya-bean meal and formaldehyde-treated soya-bean meal as chief protein sources) supplying 17, 26, 34, 39, 54 and 82 g UDP per kg DM and concentrations of RDP up to 1·5 times ARC recommendations. There was no effect of UDP on either DMI or FCE but positive, linear responses in LWG to both UDP concentration (P = 0·019) and UDP intake (P = 0·010). In both experiments, mean DMI exceeded that predicted by ARC (1980) (P < 0·05). LWG predicted by the ME system and the ARC protein scheme was not significantly different from observed LWG for non-implanted steers, but was an underestimate of observed LWG for implanted steers (P = 0·002 in experiment 1, P < 0·001 in experiment 2). Observed responses in LWG to UDP concentration were also poorly predicted (regressions of observed on predicted LWG: experiment 1, r2 = 0·12, residual s.d. = 0·126; experiment 2, r2 = 0·004, residual s.d. = 0·119). It was concluded that oestradiol-17p has significant effects on responses in LWG to UDP, which are not predicted by the current ARC (1980, 1984) protein nutrition scheme.


1986 ◽  
Vol 43 (3) ◽  
pp. 477-484 ◽  
Author(s):  
M. F. Fuller ◽  
J. Wood ◽  
A. C. Brewer ◽  
K. Pennie ◽  
R. MacWilliam

ABSTRACTOne hundred and ninety-two individually fed pigs, half of them castrated males and half females, were used to examine the effects of reducing the concentration of dietary lysine below the value of 0·84 g/MJ digestible energy suggested by the Agricultural Research Council (1981). Lysine was varied by altering the inclusion of either free lysine hydrochloride or soya-bean meal. Over the whole experiment from 18 to 65 kg live weight, there were significant reductions in live-weight gain and increases in food: gain ratio in response to reducing lysine concentration; these changes were greater with soya-bean meal than with free lysine. During later growth (45 to 65 kg) alterations in lysine concentration had no significant effect.For pigs given soya-bean supplements, both the live weights after fasting and the carcass weights were significantly lower than for those given free lysine hydrochloride, suggesting that at least part of the difference in growth between pigs given free lysine hydrochloride and soya-bean was attributable to this difference. The food required per kg carcass weight gain, or per kg lean tissue gain, was not affected by the source of lysine.In a subsidiary experiment using nitrogen balance, supplements of five amino acids in addition to lysine did not increase nitrogen retention, providing further evidence that lysine was the only amino acid responsible for the response seen with soya-bean meal. It was concluded that for growing pigs with daily food intakes of 1·5 to 1·8 kg, a lysine concentration of at least 0·81 g/MJ digestible energy is required to maximize performance.


Author(s):  
M. Ellis ◽  
D.S. Parker

Synthetic lysine and fat supplements are widely used in diets for growing pigs. However, there is evidence to suggest that free lysine is more rapidly absorbed than protein bound amino acids resulting in reduced growth performance particularly under infrequent feeding regimes. In addition, the incorporation of conventional fat supplements into cereal based diets is problematic particularly where on-farm mixing of feed is carried out. One potential solution to both these problems is to use fat coated synthetic lysine sources which have recently become available and two such materials were evaluated in this study.Four dietary treatments were compared. These were a control diet (C) with soya bean meal as the protein supplement and diets in which 0.3 of the total lysine was supplied from either synthetic lysine (S) or synthetic lysine coated with a single (SC) or a double layer(DC) of hardened soya oil. All diets were barley based and were formulated to contain the same level of total lysine (0.01) and hardened soya oil.


1989 ◽  
Vol 48 (1) ◽  
pp. 157-179 ◽  
Author(s):  
S. Green ◽  
T. Kiener

ABSTRACTIn order to determine the relative digestibilities of nitrogen and amino acids in foodstuffs for pigs and poultry, and the effects of manufacturing methods, equal quantities of soya-bean meal, sunflower meals [hulled (sunflower meal 1) and dehulled (sunflower meal 2)], meat meals [made with (meat meal 1), and without (meat meal 2), blood added at 250 g/kg meat tissue (wet weights)] and rapeseed meals [seeds heated at 80°C (rapeseed meal 1) or 100°C (rapeseed meal 2)] were mixed with protein-free ingredients. The diets were given to five growing pigs with ileo-rectal anastomoses, and, by crop-intubation, to 12 caecectomized and 12 intact cocks. Excreta were collected over 48-h periods. Endogenous excretion was estimated by giving protein-free diets.In the order, soya-bean meal, sunflower meals 1, and 2, meat meals 1, and 2, rapeseed meals 1, and 2, true digestibilities were: with pigs, of nitrogen, 0·81, 0·80, 0·79, 0·64, 0·79, 0·73, 0·70 (s.e.d. 0·030), of lysine, 0·84, 0·83, 0·84, 0·65, 0·84, 0·76, 0·72 (s.e.d. 0·032); with caecectomized birds, of nitrogen, 0·92, 0·91, 0·91, 0·66, 0·78, 0·74, 0·75 (s.e.d. 0·018), of lysine 0·92, 0·91, 0·93, 0·62, 0·79, 0·70, 0·70 (s.e.d. 0·020); with intact birds, values were similar to those with caecectomized birds for soya-bean, and the sunflower meals, but lesser for meat meals 1 and 2; the solubilities of nitrogen in pepsin were 0·96, 0·92, 0·93, 0·80, 0·89, 0·87, 0·87.Two hundred and eighty pigs (initial live weights 10 kg) were used to compare growth response to free lysine with that to lysine in soya-bean meal and sunflower meal 2. Lysine availabilities, assessed by analyses of regressions of live-weight gain against lysine intake were 0·82 (s.e. 0·12) for soya-bean meal, and 0·82 (s.e. 0·18) for sunflower meal 2.Amino acid digestibilities of the sunflower meals were similar to those of soya-bean meal, and were not influenced by dehulling; values for the rapeseed meals were lower, and unaffected by differences in heating severity; values for the meat meal were reduced by blood addition. Values differed between pigs and poultry, but there was consistency in the extent to which each species discriminated between some foodstuffs. The pepsin test was insensitive. The large standard errors associated with availability values prevented meaningful comparisons with digestibility values.


1987 ◽  
Vol 44 (1) ◽  
pp. 75-82 ◽  
Author(s):  
J. A. Stedman ◽  
R. Hill

ABSTRACTThe voluntary food intake in a limited period, 30 or 60 min after morning and afternoon feeds, and during 24 h, by lambs and calves given diets containing rapeseed meal (RSM) or soya bean meal (SBM) as the only protein supplement was determined. Four rapeseed meals were compared, one from seed of British-grown winter Brassica napus varieties (BRSM), and the others from seeds of the varieties Tower (TRSM) and Loras (LRSM), or from seeds of Canadian spring sown varieties (Canola; CRSM). The effects on food intake of treating BRSM with heat and ammonia, steam, steam and ammonia, or calcium hydroxide and ammonia were also determined.In lambs, intakes in a limited period of BRSM and CRSM were significantly lower than that of SBM, and there were no differences between intakes of BRSM and CRSM. In calves, intake in a limited period of BRSM was significantly lower than intake of SBM, TRSM and LRSM: those of TRSM and LRSM were lower, but not significantly so, than that of SBM. Treatment of BRSM with steam, steam and ammonia or calcium hydroxide and ammonia, increased food intake significantly but by small amounts: intakes of the treated meal diets were markedly lower than that of the control SBM diet. The glucosinolate concentration of untreated RSM influenced intake in calves but not in lambs. The effects of treatment of RSM on intake were not closely related to changes in glucosinolate concentration.


1985 ◽  
Vol 53 (3) ◽  
pp. 691-708 ◽  
Author(s):  
J. A. Rooke ◽  
H. A. Greife ◽  
D. G. Armstrong

1. In a 4 × 4 Latin square experiment four cattle were given in two meals per d diets consisting of (g/kg dry matter (DM)) 500 barley, 400 grass silage and 100 soya-bean meal. The diets were given at either 1.15 (L) or 2.3 times (H) maintenance energy requirements and the soya-bean meal was either untreated (U) or formaldehydetreated (T).2. A 24 h collection of duodenal digesta and a 7 d collection of faeces were made using chromium sesquioxide for flow estimation and 35S as a marker of microbial nitrogen entering the small intestine. Samples of rumen fluid were also taken for estimation of lumen pH, ammonia and volatile fatty acid concentrations.3. Spot samples of duodenal digesta were obtained after administration of Cr2O3,-mordanted silage-fibre and soya-bean meal, to determine the rates of outflow of these markers from the rumen. Similar samples were also obtained after cessation of a continuous intraruminal infusion of ruthenium phenanthroline, 35S and CoEDTA.4. Incubations of each feedingstuff in porous synthetic fibre (psf) bags were carried out in the rumen and the rates of N disappearance from the bags determined.5. Increasing DM intake significantly ( P < 0.001) increased the quantities of organic matter (OM), total N and amino acid-N entering the small intestine and amounts subsequently voided in the faeces. Apparent digestibilities of OM and N were unaffected by DM intake; the proportions of total digestible OM digested in the rumen were significantly lower (P < 0.01) at the higher level of DM intake.6. Formaldehyde treatment of the soya-bean meal increased the quantities of N entering the small intestine; these increases were not significant.7. Increased DM intake increased the quantities of both microbial N (P < 0.001) and undegraded feed N (P < 0.01) entering the small intestine; HCHO-treatment also significantly (P < 0.05) increased the quantities of undegraded feed N entering the small intestine. The efficiency of microbial N synthesis within the lumen was not significantly affected by dietary treatments whereas apparent feed N degradability was reduced significantly ( P < 0.05) both by increasing DM intake and by HCHO-treatment of the soya-bean meal.8. Rates of disappearance of N from psf bags in the rumen were different for different feedingstuffs. However, for a given feedingstuff, the rate of N disappearance was not affected by the diets fed.9. The rates of decline in marker concentrations measured in duodenal digesta were significantly increased as DM intake increased with the exception of Cr2O2-soya-bean meal. The markers could be ranked (P < 0.05) in the following order of increasing outflow rate: ruthenium phenanthroline, 35S-labelled amino acids and Cr2O2-silage fibre < Cr2O3-soya-bean meal < CoEDTA.10. Estimates of the degradabilities of feedingstuffs were calculated from N disappearance rates from psf bags and either experimentally determined outflow rates or those proposed by the Agricultural Research Council (1984). Such estimates for the degradability of the whole diet were then compared with those determined in vivo using 35S as a marker.


1994 ◽  
Vol 71 (4) ◽  
pp. 531-541 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen

Two experiments were conducted to determine the utilization of ileal digestible isoleucine by growing pigs. In the first, the apparent ileal digestibility of amino acids in cottonseed meal, lupin-seed meal and soya-bean meal was determined in pigs fitted with‘T‘-shaped cannulas. In the second, three isoleucine-deficient diets were formulated to 0.23 g ileal digestible isoleucine/MJ digestible energy (DE) with the three protein concentrates contributing the only source of isoleucine in sucrose-based diets. An additional three diets were formulated with supplements of isoleucine to confirm that isoleucine was limiting in the first three diets. The growth performance and retention of isoleucine by pigs given the six diets over the 20–45 kg growth phase were then determined. The apparent ileal digestibility of isoleucine in the three protein concentrates (proportion of total) was: cottonseed meal 0.68, lupin-seed meal 0.86, soya-bean meal 0.86. There were no significant differences (P > 0.05) in growth rates (g/d) and crude protein deposition rates (g/d) of the pigs given the three diets formulated to 0.23 g ileal digestible isoleucine/MJ DE: cottonseed meal 590, 84; lupin-seed meal 613, 87; soya-bean meal 594, 91 (SEM 13.0, 2.9) respectively. The response of pigs to the addition of isoleucine confirmed that isoleucine was limiting in these diets. The proportion of ileal digestible isoleucine retained by pigs given the cottonseed meal (0.65) was slightly lower than that retained by pigs given soya-bean meal (0.73; P < 0.05). These results indicate that values for the ileal digestibility of isoleucine in protein concentrates more closely reflect the proportion of isoleucine that can be utilized by the pig than occurs for other amino acids such as lysine, threonine and methionine.


1972 ◽  
Vol 15 (2) ◽  
pp. 117-125 ◽  
Author(s):  
J. L. Davies ◽  
I. A. M. Lucas

SUMMARYAllowances of about 1·4M, 1·8M, 2·2M, 2·6M, 3·OM, and 3·4M (M = feed required for maintenance) were compared for pigs growing over the live-weight ranges 20·40, 40·60, 60·80, and 80·100 kg, with feed conversion efficiency (FCE) as the main response criterion.Response to increased digestible energy (DE) allowance was curvilinear; FCE improved rapidly up to 2·2M-2·6M, and changed much less with intakes above 2·6M. It is concluded that optimum FCE occurs at about 2·7M for pigs of 30 kg and at about 3·OM for pigs of 50, 70 and 90 kg.Allowances of dietary DE suggested by the Agricultural Research Council correspond to 2·56M, 2·95M, 3·04M and 3·08M for pigs of 30, 50, 70 and 90 kg live weight, respectively. It appears that these are near to the optima in promoting the best FCE.Changes of −25%, −15%, −5%, +5% and +15% in daily intake around the optimum levels caused FCE to deteriorate by averages of about 13%, 4·5%, <1%, <1% and 4·5% respectively. Daily weight gains would then change by about −34%, −18%, −5%, +4% and +10%, respectively.


1994 ◽  
Vol 71 (3) ◽  
pp. 345-360 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen ◽  
D. R. Baigent

The ileal digestibility of tryptophan for growing pigs was determined for cottonseed, meat-and-bone and soya-bean meals. Tryptophan in the food and digesta was measured by two analytical procedures (NaOH hydrolysis and colorimetric estimation (method 1) and LiOH hydrolysis and HPLC determination (method 2)). The results were respectively: cottonseed meal 0.46, 0.81; meat-and-bone meal 0.55, 0.65; soya-bean meal 0.74, 0.90. In the first experiment the values for method 1 were shown to be inapplicable to pigs. In a second experiment three tryptophan-deficient diets (005 g ileal digestible tryptophan/MJ digestible energy (DE)) were formulated using values from method 2 for cottonseed meal, meat-and-bone meal plus L-tryptophan and soya-bean meal respectively as the only sources of tryptophan in the diets. This experiment was terminated after 28 d as overall growth performance of the pigs was very low. A third experiment was conducted in a similar manner to Expt 2 except that the diets were formulated to 0.065g ileal digestible tryptophan/MJ DE and growth responses and tryptophan retention were assessed over the 20–45 kg growth phase. Growth rates (g.d) of the pigs given the three diets were significantly different (P < 0.01): cottonseed meal 393, meat-and-bone meal plus L-tryptophan 531, soya-bean meal 437 (SED 39.0). Tryptophan retention (as a proportion of ileal digestible tryptophan intake) was significantly different (P < 0.05): cottonseed meal 0.51, meat-and-bone meal plus L-tryptophan 0.49, soya-bean meal 0.41. These results indicate (1) that the colorimetric technique for assessing tryptophan was inapplicable and (2) that ileal digestible values for tryptophan were not suitable for formulating diets containing heat-processed proteins, possibly due to absorption of some of the tryptophan in a form that was non-utilizable, and/or to underestimation of total tryptophan in the protein concentrates.


1978 ◽  
Vol 18 (95) ◽  
pp. 781
Author(s):  
EB Greer ◽  
CE Lewis

A 2 x 2 x 3 x 3 factorial experiment with 72 growing pigs examined the effect of adding salt, calcium and phosphorus (Ca + P) and trace minerals (TM-manganese, zinc, iron and copper) to a wheat/soybean meal diet. Gilts and barrows were individually fed at restricted intakes from 20 kg liveweight. Pigs required both salt and Ca + P to reach slaughter at 73 kg. Without these minerals they stopped growing; lameness and broken bones were also common. All pigs fed salt plus the higher level of Ca + P (0.84 and 0.57 per cent, respectively) completed the experiment but only half those fed salt plus the lower level (0.42 and 0.29 per cent Ca + P, respectively) did so. There was a response to salt within three weeks. In the first four weeks, 0.125 per cent added salt improved growth by 26.5 per cent and feed conversion ratio by 21 per cent. The results indicated that growing pigs require less sodium than currently estimated (Agricultural Research Council). A response to both levels of supplementary Ca + P was seen after about seven weeks with salt and nine weeks without salt. In the first eight weeks of the experiment there were no differences in performance between the two levels of added Ca + P when salt was also used. Between 8 and 12 weeks, pigs fed the higher level grew 17 per cent faster. Although 0.84 + 0.57 per cent Ca + P increased rib Ca and P levels, foot and joint abnormalities showed that these levels were insufficient for normal bone development. This suggests that the Agricultural Research Council estimates of Ca and P requirements for restrictively fed growing pigs are low. TM supplementation did not affect performance when both salt and Ca + P were added to the diet.


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