scholarly journals Effect of undegradable intake protein supplementation on urea kinetics and microbial use of recycled urea in steers consuming low-quality forage

2008 ◽  
Vol 101 (2) ◽  
pp. 225-232 ◽  
Author(s):  
Tryon A. Wickersham ◽  
Evan C. Titgemeyer ◽  
Robert C. Cochran ◽  
Erin E. Wickersham
Keyword(s):  
Body Weight ◽  
Gastrointestinal Tract ◽  
Latin Square ◽  
Protein Supplementation ◽  
Urea Synthesis ◽  
Urea Kinetics ◽  
Undegradable Intake Protein ◽  
Urea Recycling ◽  
Microbial N

We evaluated the effect of undegradable intake protein (UIP) on urea kinetics and microbial incorporation of urea-N in ruminally and duodenally fistulated steers (n4; 319 kg) providedad libitumaccess to grass hay in a 4 × 4 Latin square. Casein was continuously infused abomasally in amounts of 0, 62, 124 and 186 mg N/kg body weight per d to simulate provision of UIP. Periods were 13 d long with 7 d for adaptation and 6 d for collection. Jugular infusion of [15N15N]urea followed by determination of urinary enrichment of [15N15N]urea and [14N15N]urea was used to measure urea kinetics. Forage and N intake increased (quadratic,P < 0·02) with increasing UIP. Urea synthesis was 27·1, 49·9, 82·2 and 85·8 g urea-N/d for 0, 62, 124 and 186 diets, respectively (linear,P < 0·01). The proportion of urea synthesis that entered the gastrointestinal tract was 0·96 for steers receiving no UIP and decreased linearly (P = 0·05) to a low of 0·89 for steers receiving 186. The amount of urea entering the gastrointestinal tract was least for 0 (26·3) and increased (linear,P < 0·01) to 48·7, 77·2 and 76·6 g urea-N/d for 62, 124 and 186 diets, respectively. Microbial incorporation of recycled urea-N increased quadratically (P = 0·04) from 13·9 for 0 to 47·7 g N/d for 124. The proportion of microbial N derived from recycled urea increased (quadratic,P = 0·05) from 0·31 to 0·58 between 0 and 124 and dropped to 0·44 for 186 mg N/kg body weight per d. UIP increased intake of hay and provided a N source for ruminal microbes via urea recycling.

Urea supplementation in rumen and post-rumen for cattle fed a low-quality tropical forage

2020 ◽  
Vol 124 (11) ◽  
pp. 1166-1178
Author(s):  
Cristhiane V. R. de Oliveira ◽  
Tadeu E. Silva ◽  
Erick D. Batista ◽  
Luciana N. Rennó ◽  
Fabyano F. Silva ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Continuous Infusion ◽  
Latin Square ◽  
N Losses ◽  
Pulse Dose ◽  
Tropical Forage ◽  
Abomasal Infusion ◽  
Urea Kinetics ◽  
N Metabolism ◽  
Microbial N

AbstractWe evaluated the differences between the supplementation of urea in rumen and/or abomasum on forage digestion, N metabolism and urea kinetics in cattle fed a low-quality tropical forage. Five Nellore heifers were fitted with rumen and abomasum fistulas and assigned to a Latin square design. The treatments were control, continuous infusion of urea in the abomasum (AC), continuous infusion of urea in the rumen, a pulse dose of urea in the rumen every 12 h (PR) and a combination of PR and AC. The control exhibited the lowest (P < 0·10) faecal and urinary N losses, which were, overall, increased by supplementation. The highest urinary N losses (P < 0·10) were observed when urea was either totally or partially supplied as a ruminal pulse dose. The rumen N balance was negative for the control and when urea was totally supplied in the abomasum. The greatest microbial N production (P < 0·10) was obtained when urea was partially or totally supplied in the abomasum. Urea supplementation increased (P < 0·10) the amount of urea recycled to the gastrointestinal tract and the amount of urea-N returned to the ornithine cycle. The greatest (P < 0·10) amounts of urea-N used for anabolism were observed when urea was totally and continuously infused in the abomasum. The continuous abomasal infusion also resulted in the highest (P < 0·10) assimilation of microbial N from recycling. The continuous releasing of urea throughout day either in the rumen or abomasum is able to improve N accretion in the animal body, despite mechanism responsible for that being different.

DETERMINATION OF THE METHIONINE REQUIREMENT OF GROWING PIGS USING SERUM FREE AMINO ACIDS

1972 ◽  
Vol 52 (1) ◽  
pp. 163-169 ◽  
Author(s):  
M. O. KEITH ◽  
D. A. CHRISTENSEN ◽  
B. D. OWEN
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Free Amino Acids ◽  
Free Amino ◽  
Latin Square ◽  
Growing Pigs ◽  
Performance Data ◽  
Serum Free ◽  
Methionine Concentration

Six littermate Yorkshire gilts averaging 18 kg body weight were fed a semipurified diet containing six graded levels of methionine during six 4-day feeding periods in a Latin square design. Serum methionine concentrations were determined at the end of each period. Plotting serum methionine concentration against dietary methionine intake showed the methionine requirement to be 0.46% of the diet; this estimate was substantiated by animal performance data.

Formaldehyde treatment of concentrate diets for sheep. II.* Effect on urea synthesis and excretion

1977 ◽  
Vol 28 (6) ◽  
pp. 1069 ◽  
Author(s):  
GJ Faichney ◽  
GA White
Keyword(s):  
Gastrointestinal Tract ◽  
Protein Level ◽  
Consistent Pattern ◽  
Urea Synthesis ◽  
Plasma Urea ◽  
Urea Excretion ◽  
Protein Levels ◽  
Formaldehyde Treatment ◽  
Treatment Changes ◽  
Urea Recycling

The synthesis of urea, its return to the gastrointestinal tract and excretion in the urine were studied in sheep given concentrate diets, untreated or treated with formaldehyde, containing four different protein levels. Rumen ammonia and plasma urea levels increased with protein level and decreased with formaldehyde treatment. Changes in urea excretion were closely related to changes in plasma urea levels. There was a relationship between urea synthesis and rumen ammonia levels, but differences in the amount of urea returned to the gastrointestinal tract did not follow a consistent pattern. When expressed as a percentage of urea synthesized, urea recycling decreased as protein level increased but tended to increase when the diets were treated. The results are consistent with an appreciable fraction of the nitrogen returned to the rumen being in forms other than urea. _________________ *Part I, Aust. J. Agric. Res., 28: 1055 (1977).

Utilization of concentrate supplements containing varying levels of cassava leaf pellet by growing goats fed a basal diet of pangola hay

2017 ◽  
Author(s):  
Pramote Paengkoum ◽  
Sorasak Thongpea  ◽  
Siwaporn Paengkoum
Keyword(s):  
Body Weight ◽  
Soybean Meal ◽  
Dry Matter ◽  
Latin Square ◽  
Basal Diet ◽  
Ruminal Fermentation ◽  
N Supply ◽  
Growing Goats ◽  
Average Body ◽  
Microbial N

The objective of this study was to evaluate the effect of varying levels of cassava leaf pellet (CLP) on feed intake, digestibility and rumen fermentation of goats fed with pangola hay as roughages. Eight growing crossed (Thai native x Anglo-Nubian) goats between 10 to 12 months of age and pre-trial average body weight of 18.5 + 2.5 kg were allotted into two groups on the basis of body weight in a randomized double 4 x 4 Latin square design to investigate the utilization of concentrate mixtures of varying levels of CLP. The four concentrate mixtures had CLP included at 0% (control), 10 % (CLP1), 20% (CLP2) and 30% (CLP3) in concentrate as replacing soybean meal (SBM) with CLP as 25 %CP, 50%CP and 75%CP, respectively.  On average, all parameters measured, including dry matter intake (DMI), nutrients digestibility, ruminal ammonia-N (NH3-N), ruminal total volatile fatty acid (total VFA), individual VFAs concentration (mM/L), microbial N supply, and N retention increased (P less than 0.05) with the addition of CLP in concentrate up to 20% (CLP2) in concentrate, thereafter  decreased (P less than 0.05) in goats fed CLP 30% (CLP3). The results indicated that replacing SBM with CLP as 50 %CP (CLP2), resulted in higher animal performance as a consequence of improved ruminal fermentation, microbial yield and N balance,  but decreased (P less than 0.05) with 30% CLP diet. It is concluded that cassava leaf pellet can successfully replace soybean meal 50% CP as a protein source in growing goats.

Effect of ammoniation or protein supplementation of barley straw on digestion and purine derivative excretion in sheep

1997 ◽  
Vol 64 (3) ◽  
pp. 433-439 ◽  
Author(s):  
P. O. Mawuenyegah ◽  
L. Warly ◽  
T. Harumoto ◽  
T. Fujihara
Keyword(s):  
Evaluation Method ◽  
Latin Square ◽  
Protein Supplementation ◽  
Soya Bean ◽  
Barley Straw ◽  
Microbial Protein ◽  
Purine Derivative ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
Microbial N

AbstractAn experiment was conducted to examine the effect of ammonia treatment or soya-bean meal supplementation on rumen and lower tract digestion and urinary purine-derivative excretion in sheep given barley straw-based diets. Four wethers each fitted with simple rumen and duodenal cannulae were randomly allocated to four diets in a 4×4 Latin-square design. The diets were, untreated barley straw + molasses meal (diet 1), ammonia-treated barley straw + molasses meal (diet 2), untreated barley straw + soya-bean meal + molasses meal (diet 3) and ammonia-treated barley straw + soya-bean meal + molasses meal (diet 4). Dry-matter (DM) intake, total tract neutral- and acid-detergent fibre apparent digestibility and faecal nitrogen (N) output were higher for diets 2 and 4. Microbial protein yield, determined from total urinary purine-derivative excretion, was also high in sheep on diets 2 and 4. However, supplementation with soya-bean meal in diet 4 reduced DM and fibre digestion but increased intake and microbial synthesis. Despite the lower microbial protein synthesis for diet 3, total tract N digestion was high for the diet, an indication of higher nutrient absorption in the lower tract. The above results indicated that there was a substantial increase in rumen microbial N yield from ammoniated straw-based diets. However, not all the N was made available to the animals. Some of the microbial N absorbed in the lower tract may have been overestimated by the use of the purine derivative-microbial N evaluation method. On the other hand, when protein supplements were given the amount of microbial protein available to the ruminant through absorption in the lower tract may have been underestimated by urinary purine derivatives.

scholarly journals Evidence that lamprey GnRH-III does not release FSH selectively in cattle

Reproduction ◽  
2004 ◽  
Vol 127 (1) ◽  
pp. 35-43 ◽  
Author(s):  
M Amstalden ◽  
D A Zieba ◽  
M R Garcia ◽  
R L Stanko ◽  
T H Welsh ◽  
...  
Keyword(s):  
Body Weight ◽  
Luteal Phase ◽  
Primary Cultures ◽  
Latin Square ◽  
Weak Competitor ◽  
Hormonal Milieu ◽  
Selective Effects

Experiments were performed to test the hypothesis that lamprey GnRH-III (lGnRH-III) selectively releases FSH. Primary cultures of bovine adenohypophyseal cells were treated with mammalian GnRH (mGnRH) and lGnRH-III (10−9, 10−8, 10−7 and 10−6 M) or control media in Experiment 1. All doses of mGnRH and the two highest doses of lGnRH-III stimulated (P < 0.001) a non-selective release of LH and FSH. In Experiments 2–4, Latin Square designs were utilized in vivo to examine whether physiological and hormonal milieu regulate putative selective effects of lGnRH-III. In Experiments 2 and 3, ovariectomized cows with basal levels of estradiol only (Experiment 2) or in combination with luteal phase levels of progester-one (Experiment 3) were injected with mGnRH and lGnRH-III (0.055, 0.11, 0.165 and 1.1 μg/kg body weight (BW) and saline. All doses of mGnRH released (P < 0.001) LH and FSH, but only the highest dose of lGnRH-III stimulated (P < 0.001) a non-selective release of both LH and FSH (Experiment 3). For Experiments 4A and 4B, intact, mid-luteal phase cows were injected with mGnRH and lGnRH-III (1.1 μg/kg BW; Experiment 4A), lGnRH-III (1.1 and 4.4 μg/kg BW; Experiment 4B) and saline. As before, mGnRH released (P < 0.001) both LH and FSH at all doses. In contrast, lGnRH-III at the highest dose released (P < 0.001) LH but not FSH. These findings suggest that lGnRH-III may act as a weak competitor for the mGnRH receptor and do not support the hypothesis that it selectively releases FSH in cattle.

scholarly journals Relationship between Body Mass Index and Tooth Decay in a Population of 3–6-Year-Old Children in Iran

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Leila Shafie Bafti ◽  
Maryam Alsadat Hashemipour ◽  
Hamidreza Poureslami ◽  
Zeinab Hoseinian
Keyword(s):  
Body Weight ◽  
Analytical Study ◽  
Deciduous Teeth ◽  
Tooth Decay ◽  
Cross Sectional ◽  
Dmft Index ◽  
The Mean ◽  
Iranian Children ◽  
The Relationship

The aim of the present study was to evaluate the relationship between BMI and tooth decay in a population of Iranian children. In this cross-sectional descriptive/analytical study, 1482 children were selected from kindergartens and preschool centers in Kerman, Iran. The children underwent examination of deciduous teeth (using the dmft index) after determination of height and weight for calculation of BMI. The relationship between BMI (after adjustment for age) and dmft was determined using Poisson’s regression model. The mean of dmft in children with normal BMI was 1.5-fold that in subjects with extra body weight. Age had a significant effect on dmft. In addition, dmft was higher in boys compared to girls. The results of the present study showed that caries rate in the deciduous teeth of 3–6-year-old children decreases with an increase in body weight.

PSX-B-10 Effect of undigested neutral detergent fiber concentration and forage inclusion rate on ruminal pH, reticular motility, and total tract permeability for finishing beef heifers

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 457-458
Author(s):  
Murillo C Pereira ◽  
Karen A Beauchemin ◽  
Tim A McAllister ◽  
Wenzhu Yang ◽  
Joyce Van Donkersgoed ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Dry Matter ◽  
Latin Square ◽  
Barley Grain ◽  
Neutral Detergent Fiber ◽  
Fiber Concentration ◽  
Eating Time ◽  
Ruminal Ph ◽  
Contraction Duration ◽  
Gastrointestinal Permeability

Abstract This study evaluated the effects of undigested neutral detergent fiber (uNDF) concentration and forage inclusion (FI) rate on dry matter (DM) intake, ruminal pH, reticular contractions, and gastrointestinal permeability for finishing beef cattle. Five ruminally cannulated Hereford′Simmental heifers (699±69.1 kg) were used in an incomplete 6×6 Latin square (26-d periods) with a 2×3 factorial treatment arrangement. Barley grain-based diets were formulated using barley silage or wheat straw to provide low or high uNDF (7.1 vs. 8.5% DM) with forage proportions of 5, 10, or 15% of dietary DM. Dry matter intake (P ≥ 0.10) and eating time (P ≥ 0.13) were not affected by uNDF, FI, or uNDF′FI. With low uNDF diets, increasing FI numerically (P = 0.02) increased rumination time (min/d); while, with high uNDF diets, rumination time increased with 5 to 10% FI, but not thereafter (P = 0.03). Mean ruminal pH was not affected by uNDF (6.17 vs. 6.19; P = 0.08), but increased with increasing FI (6.04b, 6.23a, and 6.28a; P = 0.02). Duration of ruminal pH &lt; 5.5 was not affected by uNDF but tended (P = 0.07) to be reduced with increasing FI. High uNDF diet tended to increase the frequency of reticular contractions (1.43 vs. 1.51 contractions/min; P = 0.07) but decreased the contraction duration (13.2 vs. 14.1 sec; P = 0.04). Increasing FI increased contraction frequency (1.39b, 1.50a, and 1.53a contractions/min; P = 0.03) and tended to reduce contraction duration as forage increased from 5 to 10 and 15% (14.3, 13.1, and 13.6 sec; P = 0.07). Feeding high uNDF decreased (P = 0.05) permeability of the gastrointestinal tract based on the appearance of Cr-EDTA in urine following an intra-ruminal dose. Increasing FI tended to reduce gastrointestinal tract permeability (P = 0.06). Limited interactions indicate that uNDF and FI act independently suggesting that increasing dietary uNDF, without increasing FI rate, can stimulate frequency of reticulo-ruminal contractions and reduce gastrointestinal permeability for finishing cattle.

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