scholarly journals Effects of fish oil and starch added to a diet containing sunflower-seed oil on dairy goat performance, milk fatty acid composition and in vivo Δ9-desaturation of [13C]vaccenic acid

2010 ◽  
Vol 104 (3) ◽  
pp. 346-354 ◽  
Author(s):  
Laurence Bernard ◽  
Julien Mouriot ◽  
Jacques Rouel ◽  
Frédéric Glasser ◽  
Pierre Capitan ◽  
...  
Keyword(s):  
Fish Oil ◽  
Milk Yield ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Vaccenic Acid ◽  
Dairy Goat ◽  
Sunflower Seed Oil ◽  
Δ9 Desaturase ◽  
Dietary Treatments

The potential benefits on human health have prompted an interest in developing nutritional strategies for specifically increasing rumenic acid (RA) in ruminant milk. The aims of the present study were to (i) compare two dietary treatments with lipid supplements on milk yield and composition, (ii) measure the in vivo Δ9-desaturation of vaccenic acid (VA) to RA using 13C-labelled VA and (iii) determine the effect of the dietary treatments on this variable. Treatments were 90 g sunflower-seed oil (SO) per d or 60 g sunflower-seed oil and 30 g fish oil per d plus additional starch (SFO), in a grassland hay-based diet given to eight Alpine goats in a 2 × 2 cross-over design with 21 d experimental periods. Milk yield and composition were similar between treatments. Goats fed SFO had higher milk 6 : 0–16 : 0 concentration, lower milk ΣC18 concentrations and showed no effect on milk VA and RA, compared with SO. At the end of the experiment, intravenous injection of 1·5 g [13C]VA followed by measurements of milk lipid 13C enrichment showed that in vivo 31·7 and 31·6 % of VA was Δ9-desaturated into milk RA in the caprine with the SO and SFO treatments, respectively. The expression of genes encoding for Δ9-desaturase (or stearoyl-CoA desaturase; SCD1, SCD5) in mammary tissues and four milk Δ9-desaturation ratios were similar between treatments. In conclusion, the present study provides the first estimates of in vivo endogenous synthesis of RA (63–73 % of milk RA) from VA in goats, and shows no difference between the two lipid supplements compared.

scholarly journals The vitamin E nutritional status of rats fed on diets high in fish oil, linseed oil or sunflower seed oil

1994 ◽  
Vol 72 (1) ◽  
pp. 127-145 ◽  
Author(s):  
Sandra R. Farwer ◽  
Bernardus C. J. Der Boer ◽  
Edward Haddeman ◽  
Gerardus A. A. Kivits ◽  
Antoon Wiersma ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Vitamin E ◽  
Fish Oil ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Linseed Oil ◽  
Tocopheryl Acetate ◽  
Control Groups ◽  
Sunflower Seed Oil

Twelve groups of eight rats and two control groups of sixteen rats were given semisynthetic diets with 40% energy as fat for a period of 76 d. All diets contained a minimum of 3% energy as linoleic acid and comparable basal levels of D-α- and D-γ-tocopherol. The diets varied in fat composition and in the content of DL-α-tocopheryl acetate. The diets high in polyunsaturated fatty acids (PUFA) were either rich in fish oil (FO; groups 1–4; 10% energy as fish oil PUFA), linseed oil (LN; groups 1–4; 10% energy as α-linolenic acid) or sunflower seed oil (SF; groups 1–4; 10 + 3% energy as linoleic acid). The control groups were given a diet high in monounsaturated fatty acids (MUFA; CO 1; 10 + 13% energy as oleic acid) or a diet with an ‘average’ linoleic acid content (CO 2; 8.5% energy as linoleic acid). Of each high PUFA diet three groups were supplemented with graded levels of DL-α-tocopheryl acetate. Steatitis, a sensitive histopathological indicator of vitamin E deficiency in animals fed on diets rich in fatty acids with three or more double bonds, was observed only in the adipose tissue of the FO groups, even in the group with the highest DL-α-tocopheryl acetate supplementation. Liver and serum α- tocopherol levels were found to be positively correlated and liver and serum γ-tocopherol levels negatively correlated with dietary DL-α-tocopheryl acetate. The groups on the FO diets had significantly reduced liver and serum tocopherol levels in comparison with the groups on the other high-PUFA diets. With the supplementation scheme used for the FO groups the liver α-tocopherol levels of both control groups were reached but the serum control levels were not.

scholarly journals Milk performance of dairy cows supplemented with rape seed oil, peanut oil, and sunflower seed oil

2011 ◽  
Vol 56 (No. 4) ◽  
pp. 181-191 ◽  
Author(s):  
X.J. Dai ◽  
C. Wang ◽  
Q. Zhu
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Milk Composition ◽  
Peanut Oil ◽  
Plant Oils ◽  
Sunflower Seed Oil

The objective of the study was to investigate the effects of supplementing different plant oils to the basal diet on milk yield and milk composition in mid-lactating dairy cows. Forty Chinese Holstein dairy cows averaging 120 days in milk (DIM) at the start of the experiment (body weight = 580 ± 18.2 kg; milk yield = 33.0 ± 2.00 kg/day) were used in a completely randomized block design. The animals were assigned to four dietary treatments according to DIM and milk yield, and supplemented with no oil (control), 2% rapeseed oil (RSO), 2% peanut oil (PNO) and 2% sunflower seed oil (SFO). Milk yield and milk composition (fat, protein, and lactose) were measured. Dry matter intake was similar in all treatments. The supplementation of plant oil increased milk yield, with the highest milk yield in RSO group. Percentages of milk fat, lactose, solids-not-fat and SCC were not affected by treatments except for an increase in milk protein content in oil supplemented groups. The fatty acid (FA) profile of milk was altered by fat supplementation. Feeding plant oils reduced the proportion of both short-chain (C4:0 to C12:0) and medium-chain (C14:0 to C16:1) fatty acids, and increased the proportion of long-chain (≥ C18:0) fatty acids in milk fat. The inclusion of vegetable oils increased the concentration of cis-9, trans-11 CLA. The cis-9, trans-11 CLA content in milk fat was higher from RSO to PNO and SFO was higher than the control. The TVA concentration was higher in the SFO diet, followed by PNO, RSO, and control diets. The results of this study indicated that linoleic acid was more effective in enhancing contents of TVA and CLA in milk fat than oleic acid. No significant effects of week and treatment by week interaction were found out in this study. Overall, feeding plant oils increased monounsaturated and polyunsaturated fatty acids and decreased saturated fatty acids in milk fat. In conclusion, dietary supplementation of RSO increases milk yield the most, while SFO enhances the cis-9, trans-11 CLA content in milk fat more effectively.

scholarly journals Fatty acid composition of serum lipid classes in mice following allergic sensitisation with or without dietary docosahexaenoic acid-enriched fish oil substitution

2008 ◽  
Vol 99 (6) ◽  
pp. 1239-1246 ◽  
Author(s):  
Ralph Rühl ◽  
Christin Koch ◽  
Tamás Marosvölgyi ◽  
Johanna Mihály ◽  
Florian J. Schweigert ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Fish Oil ◽  
Acid Composition ◽  
Serum Lipid ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Lipid Classes ◽  
Allergic Sensitisation ◽  
Sunflower Seed Oil

Dietary fatty acids have been shown to influence allergic sensitisation. Both n-3 and n-6 PUFA are involved in targeted mediation of inflammatory responses during allergic sensitisation and manifestation of atopic diseases. In the present experiments we investigated whether supplementation of DHA-enriched fish oil partly substituting dietary sunflower-seed oil, in comparison with sunflower-seed oil, supplemented to mice influences fatty acid composition of serum lipid classes. The effects of the two different diets were also investigated depending on allergic sensitisation. Supplementation of DHA and EPA in doses of 2 and 0·12 % (w/w) to non-sensitised and sensitised mice resulted in significantly increased percentile contributions of DHA to all lipid classes. In contrast, serum values of the n-6 PUFA arachidonic acid (AA) were significantly lower, both in non-sensitised and sensitised mice fed the DHA-enriched diet. The fatty acid composition of serum lipids also reflected allergic sensitisation: the EPA:AA ratio in TAG, cholesteryl esters and phospholipids in non-supplemented animals fell to 23, 29 and 29 % respectively of the original value after allergic sensitisation, whereas it decreased to 70, 80 and 76 % respectively only in the animals supplemented with DHA. In summary, allergic sensitisation alone decreased significantly the EPA:AA ratios in serum TAG, while concomitant supplementation of DHA-enriched fish oil ameliorated this decrease. We postulate from the present results that the amelioration of the severity of allergic sensitisation after DHA supplementation may be linked to altered ratios of the eicosanoid precursors EPA and AA as well as DHA needed for further metabolic activation to pro- or anti-inflammatory bioactive lipids.

scholarly journals Effect of the level and type of starchy concentrate on tissue lipid metabolism, gene expression and milk fatty acid secretion in Alpine goats receiving a diet rich in sunflower-seed oil

2011 ◽  
Vol 107 (8) ◽  
pp. 1147-1159 ◽  
Author(s):  
L. Bernard ◽  
C. Leroux ◽  
J. Rouel ◽  
M. Bonnet ◽  
Y. Chilliard
Keyword(s):  
Gene Expression ◽  
Lipid Metabolism ◽  
Milk Yield ◽  
Milk Fat ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Unsaturated Fatty Acids ◽  
Sunflower Seed Oil ◽  
High Level ◽  
The Impact

The potential benefits on human health have prompted an interest in developing nutritional strategies for reducing saturated and increasing specific unsaturated fatty acids (FA) in ruminant milk. The impact of the level and type of starchy concentrate added to diets supplemented with sunflower-seed oil on caprine milk FA composition and on mammary, omental and perirenal adipose, and liver lipid metabolism was examined in fourteen Alpine goats in a replicated 3 × 3 Latin square with 21 d experimental periods. Treatments were a grass hay-based diet with a high level of forage (F) or a high level of concentrate with either maize grain (CM) or flattened wheat (CW) as source of starch and supplemented with 130 g/d sunflower-seed oil. Milk yield was enhanced (P < 0·01) and milk fat content was decreased on the CM and CW diets compared with the F diet, resulting in similar milk fat secretion. Both high-concentrate diets increased (P < 0·05) milk yield of 10 : 0-16 : 0 and decreased trans-9,11-18 : 1 and cis-9, trans-11-18 : 2. The CW diet decreased (P < 0·05) the output of Σ C18 and Σ cis-18 : 1 and increased (P < 0·05) the output of trans-10-18 : 1 in milk. The expression and/or activity of fourteen proteins involved in the major lipogenic pathways in mammary tissues and of lipogenic genes in adipose and liver tissues were similar among treatments. In conclusion, high starch concentrates alter milk FA yield via mechanisms independent of changes in mammary, liver or adipose tissue lipogenic gene expression. Furthermore, data provided indications that mammary lipogenic responses to starch-rich diets differ between caprine and bovine ruminants.

scholarly journals The effects of dietary lipids on dendritic cells in perinodal adipose tissue during chronic mild inflammation

2004 ◽  
Vol 91 (6) ◽  
pp. 883-892 ◽  
Author(s):  
Christine A. Mattacks ◽  
Dawn Sadler ◽  
Caroline M. Pond
Keyword(s):  
Dendritic Cells ◽  
Adipose Tissue ◽  
Lymph Nodes ◽  
Fish Oil ◽  
Lymphoid Tissue ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Dietary Lipids ◽  
Sunflower Seed Oil ◽  
Popliteal Lymph Nodes

The effects of dietary lipids on the abundance of dendritic cells in adipose tissue in anatomically defined relationships to chronically inflamed lymph nodes were investigated in mature male rats fed plain chow or chow plus 20 % sunflower-seed or fish oil. The popliteal lymph nodes were stimulated by local subcutaneous injection of 20 μg lipopolysaccharide to both hindlegs three times/week for 2 weeks. The masses of the major adipose depots and the numbers of dendritic cells emerging from perinodal adipose tissue and samples 5 and 10 mm from the popliteal lymph nodes were measured, and those from omental and mesenteric adipose tissue around and remote from lymphoid tissue, and mesenteric and popliteal lymph nodes. Dendritic cells were most numerous in the perinodal adipose tissue, with the corresponding ‘remote’ samples containing 25–50 % fewer such cells under all conditions studied. Dietary sunflower-seed oil increased the numbers of dendritic cells by about 17 % in all adipose samples and fish oil reduced the numbers in perinodal tissue by about 5 %. The fish-oil diet diminished responses of the intra-abdominal adipose depots to local stimulation of the popliteal node. Correlations in dendritic cell numbers were stronger between perinodal samples from different depots than between remote and perinodal samples from the same depot and after the sunflower-seed-oil diet compared with fish oil. These data show that dietary lipids modulate the number of dendritic cells in lymphoid tissue-containing adipose depots and support the hypothesis that perinodal adipose tissue interacts locally with lymphoid cells.

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