scholarly journals Comparison of catechin profiles in human plasma and urine after single dosing and regular intake of green tea (Camellia sinensis)

2012 ◽  
Vol 109 (12) ◽  
pp. 2199-2207 ◽  
Author(s):  
Shing-Tack Fung ◽  
Cyrus K. Ho ◽  
Siu-Wai Choi ◽  
Wai-Yuen Chung ◽  
Iris F. F. Benzie
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Epigallocatechin Gallate ◽  
Free Form ◽  
Epicatechin Gallate ◽  
Fasting Plasma ◽  
Blood And Urine Samples ◽  
High Level ◽  
Single Dosing ◽  
Regular Intake

Green tea (Camellia sinensis) catechin profiles in plasma and urine following single dosing and regular ingestion of green tea are not clear. We performed a placebo-controlled intervention study with sixteen healthy volunteers to determine changes in total and free catechins after a single dose and following 1 week of twice-daily green tea. Blood and urine samples were collected before (fasting) and after (60 and 120 min for blood; 90 and 180 min for urine) drinking 200 ml of 1·5 % (w/v) green tea or water (n 8 each), and fasting samples were again collected after 7 d of 150 ml of 1 % (w/v) supplemental green tea or water twice daily. After a 4-week washout, subjects were crossed onto the other treatment and procedures repeated. Plasma results at 1 h post-ingestion showed elevated (P< 0·05) mean epigallocatechin gallate (EGCG; 310 (sd 117) nmol/l; all in free form), epigallocatechin (EGC; 192 (sd 67) nmol/l; 30 % free) and epicatechin gallate (ECG; 134 (sd 51) nmol/l; 75 % free). Fasting plasma after 7 d of regular intake showed increased (P< 0·05) EGCG (80 v. 15 nmol/l at baseline) and ECG (120 v. 40 nmol/l), with ≥ 90 % of both in their conjugated forms. Total EGC was < 10 nmol/l. Post-ingestion conjugation and renal loss of EGC and epicatechin were rapid and high, but were negligible for EGCG and ECG. In the green tea consumed, the content was EGCG >EGC >ECG, and the acute plasma response mirrored this. However, after chronic consumption there was almost no EGC found in fasting plasma, some EGCG was present, but a rather high level of ECG was maintained.

Versatile Health Benefits of Catechin from Green Tea (Camellia sinensis)

2019 ◽  
Vol 15 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Satheesh Babu Natarajan ◽  
Suriyakala Perumal Chandran ◽  
Sahar Husain Khan ◽  
Packiyaraj Natarajan ◽  
Karthiyaraj Rengarajan
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Health Benefits ◽  
Epigallocatechin Gallate ◽  
Extraction Methods ◽  
Major Constituent ◽  
Epicatechin Gallate ◽  
Wide Range ◽  
Green Tea Catechin ◽  
Anti Inflammatory

Background: Tea (Camellia sinensis, Theaceae) is the second most consumed beverage in the world. Green tea is the least processed and thus contain rich antioxidant level, and believed to have most of the health benefits. </p><p> Methods: We commenced to search bibliographic collection of peer reviewed research articles and review articles to meet the objective of this study. </p><p> Results: From this study, we found that the tea beverage contains catechins are believed to have a wide range of health benefits which includes neuroprotective, anti-inflammatory, antiulcer, antiviral, antibacterial, and anti-parasitic effects. The four major catechin compounds of green tea are epigallocatechin (EGC), epicatechin (EC), epigallocatechin gallate (EGCG), and epicatechin gallate (ECG), of which EGCG is the major constituent and representing 50-80% of the total catechin content. And also contain xanthine derivatives such as caffeine, theophylline, and theobromine, and the glutamide derivative theanine. It also contains many nutritional components, such as vitamin E, vitamin C, fluoride, and potassium. We sum up the various green tea phytoconstituents, extraction methods, and its medicinal applications. </p><p> Conclusion: In this review article, we have summarized the pharmacological importance of green tea catechin which includes antioxidant potential, anti-inflammatory, antimicrobial, anticancer, antidiabetic and cosmetic application.

scholarly journals Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins

2004 ◽  
Vol 48 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Cristina Paveto ◽  
María C. Güida ◽  
Mónica I. Esteva ◽  
Virginia Martino ◽  
Jorge Coussio ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Developmental Stages ◽  
Arginine Kinase ◽  
Epigallocatechin Gallate ◽  
Array Detector ◽  
Epicatechin Gallate ◽  
Active Compounds ◽  
Gallocatechin Gallate

ABSTRACT The trypanocidal action of green tea catechins against two different developmental stages of Trypanosoma cruzi is reported for the first time. This activity was assayed with the nonproliferative bloodstream trypomastigote and with the intracellular replicative amastigote parasite forms. An ethyl acetate fraction from Camellia sinensis green tea leaves, which contains most of the polyphenolic compounds and the maximal trypanocidal activity, was obtained by fractionation of the aqueous extract with organic solvents. The active compounds present in this extract were further purified by LH-20 column chromatography and were identified by high-performance liquid chromatography analysis with a photo diode array detector and gas chromatography coupled to mass spectroscopy. The following flavan-3-ols derivatives, known as catechins, were identified: catechin, epicatechin, gallocatechin, epigallocatechin, catechin gallate, epicatechin gallate, gallocatechin gallate, and epigallocatechin gallate. The purified compounds lysed more than 50% of the parasites present in the blood of infected BALB/c mice at concentrations as low as 0.12 to 85 pM. The most active compounds were gallocatechin gallate and epigallocatechin gallate, with minimal bactericidal concentrations that inhibited 50% of isolates tested of 0.12 and 0.53 pM, respectively. The number of amastigotes in infected Vero cells decreased by 50% in the presence of each of these compounds at 100 nM. The effects of the catechins on the recombinant T. cruzi arginine kinase, a key enzyme in the energy metabolism of the parasite, were assayed. The activity of this enzyme was inhibited by about 50% by nanomolar concentrations of catechin gallate or gallocatechin gallate, whereas the other members of the group were less effective. On the basis of these results, we suggest that these compounds could be used to sterilize blood and, eventually, as therapeutic agents for Chagas' disease.

Separation of epigallocatechin gallate and epicatechin gallate from tea polyphenols by macroporous resin and crystallization

2021 ◽  
Author(s):  
Li Wang ◽  
Xin Huang ◽  
Huijuan Jing ◽  
Xin Ye ◽  
Chao Jiang ◽  
...  
Keyword(s):  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Tea Polyphenols ◽  
Green Tea Polyphenols ◽  
Macroporous Resin ◽  
Epicatechin Gallate

Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) are the most abundant ester catechins of green tea polyphenols (GTPs) with numerous potential bioactivities, which have a wide application prospect in the...

scholarly journals Green tea phytocompounds targets Lansterol 14-α demethylase against ergosterol biosynthesis in Candida glabrata

2021 ◽  
Vol 12 (3) ◽  
pp. 1793-1797
Author(s):  
Priyanka Sirari ◽  
Jigisha Anand ◽  
Devvret ◽  
Ashish Thapliyal ◽  
Nishant Rai
Keyword(s):  
Green Tea ◽  
Candida Glabrata ◽  
Scientific Evidence ◽  
Epigallocatechin Gallate ◽  
Ergosterol Biosynthesis ◽  
Epicatechin Gallate ◽  
P450 Monooxygenase ◽  
Antifungal Potential ◽  
Inhibitory Potential

Green tea is credited as one of the world’s healthiest drinks with enriched antioxidants. It is known for its multi-beneficial health benefits against diabetes, blood pressure, hypertension, gastro-intestinal upset and is bestowed with significant antimicrobial potential. There are previous scientific evidence highlighting the antifungal potential of green tea and has identified it as a potential inhibitor of non-albicans Candida species. Lansterol 14-α demethylase (Erg 11) or CYP51 protein belongs to the cytochrome P450 monooxygenase (CYP) superfamily. Erg 11 is involved in ergosterol biosynthesis and has a significant role in azole drug resistance in Candida glabrata. The present study attempted to identify the inhibitory potential of green tea phytocompounds against inhibition of Erg 11 in Candida glabrata using bioinformatics tool viz., autodock vina software. Out of 15 green tea phytocompounds investigated, the study identified, Rutin (-10.5 kcal) Kaempferitrin (-9.4kcal), Epigallocatechin gallate (-10kcal), Epicatechin gallate (-8.7kcal), and Coumaroylquinic acid (-8.6kcal) acid as the potent phytocompounds which showed significant molecular interaction with Erg 11 in Candida glabrata. In attribution to the constant emergence of azole-resistant isolates, this preliminary analysis therefore, indicated the potential of green tea phytocompounds against inhibition of non-albicans Candida specific candidiasis. However, further, in vitro antimicrobial efficacy of these phytocompounds, the dose regime, drug likeliness, and cytotoxic analysis are required to be investigated and validated.

Green tea extract improves endurance capacity and increases muscle lipid oxidation in mice

2005 ◽  
Vol 288 (3) ◽  
pp. R708-R715 ◽  
Author(s):  
Takatoshi Murase ◽  
Satoshi Haramizu ◽  
Akira Shimotoyodome ◽  
Azumi Nagasawa ◽  
Ichiro Tokimitsu
Keyword(s):  
Fatty Acid ◽  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Fat Oxidation ◽  
Green Tea Extract ◽  
Endurance Capacity ◽  
Oxidation Activity ◽  
Tea Extract ◽  
High Level ◽  
Dose Dependent

Green tea contains a high level of polyphenolic compounds known as catechins. We investigated the effects of green tea extract (GTE), which is rich in catechins, on endurance capacity, energy metabolism, and fat oxidation in BALB/c mice over a 10-wk period. Swimming times to exhaustion for mice fed 0.2–0.5% (wt/wt) GTE were prolonged by 8–24%. The effects were dose dependent and accompanied by lower respiratory quotients and higher rates of fat oxidation as determined by indirect calorimetry. In addition, feeding with GTE increased the level of β-oxidation activity in skeletal muscle. Plasma lactate concentrations in mice fed GTE were significantly decreased after exercise, concomitant with increases in free fatty acid concentrations in plasma, suggesting an increased lipid use as an energy source in GTE-fed mice. Epigallocatechin gallate (EGCG), a major component of tea catechins, also enhanced endurance capacity, suggesting that the endurance-improving effects of GTE were mediated, at least in part, by EGCG. The β-oxidation activity and the level of fatty acid translocase/CD36 mRNA in the muscle was higher in GTE-fed mice compared with control mice. These results indicate that GTE are beneficial for improving endurance capacity and support the hypothesis that the stimulation of fatty acid use is a promising strategy for improving endurance capacity.

scholarly journals Volatile profiles and antioxidant activity of different cultivars of Camellia sinensis var. assamica grown in Thailand

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 354-362
Author(s):  
S. Khruengsai ◽  
T. Sripacho ◽  
P. Pripdeevech
Keyword(s):  
Antioxidant Activity ◽  
Volatile Compounds ◽  
Camellia Sinensis ◽  
Antioxidant Activities ◽  
Epigallocatechin Gallate ◽  
Dry Weight ◽  
Epicatechin Gallate ◽  
Potential Health ◽  
Linalool Oxide ◽  
Assam Tea

Tea is considered as the most consumed drink in the world containing high antioxidant capacity. In this study, the volatile compounds, the phenolic content, catechins and caffeine including antioxidant activities of 22 Camellia sinensis var. assamica (Assam tea) cultivars were investigated. The volatile compounds were investigated by GC-MS. At least forty-five volatile compounds representing 94.99-99.65% of all cultivars were identified. Limonene, trans-linalool oxide, cis-linalool oxide, linalool, and furfural were detected as the major components among these cultivars. Varied ranges were found in all Assam tea cultivars for the contents of phenolics (113.45-245.55 mg gallic acid/g dry weight), total catechins (170.03-355.59 mg/g dry weight), caffeine (0.92-3.40 mg/g dry weight), and antioxidant activities (1418.68-2728.46 µmol Trolox/g dry weight and 1448.98-2864.17 µmol Trolox/g dry weight for DPPH and ABTS assay, respectively). The antioxidant activity was correlated with phenolic compounds such as epigallocatechin gallate, epicatechin gallate, and catechin gallate. The specific differences among Assam tea cultivars are dependent on the tea cultivar and altitude which may play a significant role in breeding Assam tea cultivars in Thailand for providing its potential health benefits.

scholarly journals Matcha Green Tea Exhibits Bactericidal Activity against Streptococcus pneumoniae and Inhibits Functional Pneumolysin

Antibiotics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1550
Author(s):  
Karin Sasagawa ◽  
Hisanori Domon ◽  
Rina Sakagami ◽  
Satoru Hirayama ◽  
Tomoki Maekawa ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Green Tea ◽  
Pneumococcal Pneumonia ◽  
Epigallocatechin Gallate ◽  
Community Acquired Pneumonia ◽  
Antibacterial Drug ◽  
Pneumococcal Infections ◽  
Epicatechin Gallate ◽  
Natural Substances

Streptococcus pneumoniae is a causative pathogen of several human infectious diseases including community-acquired pneumonia. Pneumolysin (PLY), a pore-forming toxin, plays an important role in the pathogenesis of pneumococcal pneumonia. In recent years, the use of traditional natural substances for prevention has drawn attention because of the increasing antibacterial drug resistance of S. pneumoniae. According to some studies, green tea exhibits antibacterial and antitoxin activities. The polyphenols, namely the catechins epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate (ECG), and epicatechin (EC) are largely responsible for these activities. Although matcha green tea provides more polyphenols than green tea infusions, its relationship with pneumococcal pneumonia remains unclear. In this study, we found that treatment with 20 mg/mL matcha supernatant exhibited significant antibacterial activity against S. pneumoniae regardless of antimicrobial resistance. In addition, the matcha supernatant suppressed PLY-mediated hemolysis and cytolysis by inhibiting PLY oligomerization. Moreover, the matcha supernatant and catechins inhibited PLY-mediated neutrophil death and the release of neutrophil elastase. These findings suggest that matcha green tea reduces the virulence of S. pneumoniae in vitro and may be a promising agent for the treatment of pneumococcal infections.

Sign in / Sign up

Export Citation Format

Share Document