scholarly journals Values for digestible indispensable amino acid scores (DIAAS) for some dairy and plant proteins may better describe protein quality than values calculated using the concept for protein digestibility-corrected amino acid scores (PDCAAS)

2017 ◽  
Vol 117 (4) ◽  
pp. 490-499 ◽  
Author(s):  
John K. Mathai ◽  
Yanhong Liu ◽  
Hans H. Stein
Keyword(s):  
Amino Acid ◽  
Whey Protein ◽  
Protein Quality ◽  
Protein Digestibility ◽  
Protein Isolate ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Plant Proteins ◽  
Soya Flour ◽  
Indispensable Amino Acid

AbstractAn experiment was conducted to compare values for digestible indispensable amino acid scores (DIAAS) for four animal proteins and four plant proteins with values calculated as recommended for protein digestibility-corrected amino acid scores (PDCAAS), but determined in pigs instead of in rats. Values for standardised total tract digestibility (STTD) of crude protein (CP) and standardised ileal digestibility (SID) of amino acids (AA) were calculated for whey protein isolate (WPI), whey protein concentrate (WPC), milk protein concentrate (MPC), skimmed milk powder (SMP), pea protein concentrate (PPC), soya protein isolate (SPI), soya flour and whole-grain wheat. The PDCAAS-like values were calculated using the STTD of CP to estimate AA digestibility and values for DIAAS were calculated from values for SID of AA. Results indicated that values for SID of most indispensable AA in WPI, WPC and MPC were greater (P<0·05) than for SMP, PPC, SPI, soya flour and wheat. With the exception of arginine and tryptophan, the SID of all indispensable AA in SPI was greater (P<0·05) than in soya flour, and with the exception of threonine, the SID of all indispensable AA in wheat was less (P<0·05) than in all other ingredients. If the same scoring pattern for children between 6 and 36 months was used to calculate PDCAAS-like values and DIAAS, PDCAAS-like values were greater (P<0·05) than DIAAS values for SMP, PPC, SPI, soya flour and wheat indicating that PDCAAS-like values estimated in pigs may overestimate the quality of these proteins.

scholarly journals Nutritive value and metabolic effects of whey protein concentrate and hydrolysed lactose for growing pigs

1984 ◽  
Vol 56 (3) ◽  
pp. 227-238
Author(s):  
Matti Näsi
Keyword(s):  
Amino Acids ◽  
Whey Protein ◽  
Crude Protein ◽  
Nutritive Value ◽  
Mineral Metabolism ◽  
Protein Digestibility ◽  
Growing Pigs ◽  
Metabolic Effects ◽  
Whey Protein Concentrate ◽  
Protein Concentrate

In two digestibility and balance trials with growing pigs, whey protein concentrate (WPC) was compared as a protein supplement with casein (CAS) and dried skim milk (DSM), and, 30 % lactose (40 % dried whey, DW) was compared as a sugar supplement with the same amounts of hydrolysed lactose (HYLA) and sucrose (SUC). The effects of these supplements on protein and mineral metabolism of the pigs were investigated, WPC contained 42.2 % crude protein and had a high content of lysine, 8.6 g, and sulphur containing amino acids: cystine 2.8 and methionine 2.2g/16 g N, These exceeded the values for DSM. The hydrolysing degree of the enzymatically treated lactose syrup was 73 %. WPC had high crude protein digestibility, 99.1 % as compared to 95.4 for CAS and 95.0 % for DSM. Dried whey had low crude protein digestibility, 72.5 %. The amino acids in the WPC diet were highly digestible, but low values were obtained for the DW diet. On the WPC diet, nitrogen retention was higher than with the other protein supplements (P > 0.05), urinary urea excretion was low and the biological value very high. On a combination of WPC and HYLA protein utilisation was higher than on dried whole whey. On the diets supplemented with different sugars, none of the blood parameters differed statistically significantly (P > 0.05) and all values lay within the reference range. Water intake was on average 49 % greater on diets with sugar supplements than without. Urinary excretion of reducing sugars averaged 40.2, 8.3 and 6.6 g/d on the HYLA, SUC and DW diets, while on the diets without sugar supplements the values were 0.8—1.2 g/d. The following mean daily mineral retention values were obtained: P 4.0 g, Ca 5,9 g. Mg 0,4 g, Na 1.9g, K 2.9 g, Fe 27 mg, Cu 6.4 mg, Zn 65 mg and Mn 4.0 mg. The surplus Na and K on the DW diet were excreted in the urine and the pigs did not have diarrhoea.

scholarly journals Justification of unconventional protein-containing raw materials for the construction of milk chocolate formulas with increased biological value

Vestnik MGTU ◽  
2020 ◽  
Vol 23 (3) ◽  
pp. 205-213
Author(s):  
N. V. Linovskaya ◽  
E. V. Mazukabzova ◽  
O. S. Rudenko ◽  
T. V. Savenkova
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Whey Protein ◽  
Acid Composition ◽  
Raw Materials ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Milk Chocolate ◽  
Biological Value

Milk chocolate is particularly popular with different age groups. It is characterized by low protein content with a large amount of fats and carbohydrates determining the food imbalance of the product. In conditions of high-grade animal proteins deficiency the selection of high-quality protein-containing ingredients for food production is very relevant. The aim of this work is to study the protein adequacy of various components of milk chocolate to enhance its biological value. The amino acid scale method has been used to assess the biological value of proteins; it is based on the determination of amino acid (chemical) score. It has been found that the limiting biological value amino acid for classical white raw ingredients of milk chocolate (cocoa products and milk powder) is methionine + cysteine. For constructing chocolate formulas with increased biological value it is advisable to use protein-containing raw materials (whey protein concentrate, oat flour, etc.) to compensate for the limiting amino acids. The indicator of amino acids utilitarianity of proteins of raw milk chocolate components has been calculated. On the basis of the utilitarian index we have established the coefficient of utilitarian of the amino acid composition of the raw materials characterizing essential amino acids' balance. We have determined the biological value of protein and the amino acid composition imbalance coefficient. It has been found that the amino acid composition of milk and whey protein concentrates is most balanced compared to the amino acid composition of traditional protein-containing raw components of milk chocolate. The limiting acid of whey protein concentrate is valine, which makes its use in the manufacture of chocolate products more attractive compared to milk protein concentrate (the limiting amino acid is methionine + cysteine). In the group of vegetable non-conventional raw materials oat and buckwheat flour are characterized by the best indicators of biological value. The amino acid adequacy of oat flour is comparable to the qualitative protein indicators of cocoa products, the limiting amino acid is lysine. Buckwheat flour is characterized by the smallest imbalance in amino acid composition, which distinguishes the proteins of this raw material with the highest degree of digestibility compared with the proteins of all the studied protein-containing components of milk chocolate.

scholarly journals Digestible Indispensable Amino Acid Score (DIAAS) better Estimates the Protein Value of Pistachios than Digestibility Corrected Amino Acid Score (PDCAAS)

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Hannah Marie Bailey ◽  
Arianna Carughi ◽  
Hans-Henrik Stein
Keyword(s):  
Amino Acid ◽  
Unsaturated Fatty Acids ◽  
Protein Quality ◽  
Protein Digestibility ◽  
Growing Pigs ◽  
Ileal Digestibility ◽  
Indispensable Amino Acid ◽  
Older Adolescents ◽  
Adolescents And Adults ◽  
Amino Acid Score

AbstractPistachios (Pistacia Vera) are nutrient-dense foods with a healthy nutritional profile that contains fiber, unsaturated fatty acids, essential nutrients, phytochemicals and protein. However, the nutritional quality of the protein has not been characterized to date. Therefore, the objective of this study was to determine the Protein Digestibility Corrected Amino Acid Score (PDCAAS) and Digestible Indispensable Amino Acid Score (DIAAS) for raw and roasted American pistachio nuts in growing pigs. The values for apparent ileal digestibility (AID) of crude protein (CP) and the standard ileal digestibility (SID) where calculated for these proteins. The CP content was greater for raw pistachios (27.1%) compared with roasted pistachios (25.1%). The amino acid (AA) in greatest concentrations for both types of pistachio were arginine followed by leucine. The AA in the lowest concentration were tryptophan followed by methionine. The AID of CP was greater (P < 0.05) for raw pistachios than for roasted pistachios, and the AID of most indispensable AA (IAA), except arginine, isoleucine, and phenylalanine, was greater (P < 0.05) for raw pistachios. The SID of CP was greater (P < 0.05) for raw than for roasted pistachios, and the SID of all IAA, except phenylalanine, was greater (P < 0.05) for raw than for roasted pistachios. The PDCAAS value calculated for both types of pistachios was based on the requirement for children from 2 to 5 years old (FAO, 1991), whereas the DIAAS value was calculated based on the requirement for children 3 years and older, adolescents, and adults (FAO, 2013). Both raw and roasted pistachios had a PDCAAS value of 75. In contrast, the DIAAS was numerically greater for raw pistachios than for roasted pistachios with values of 86 and 83, respectively. For PDCAAS, the first limiting AA in both raw and roasted pistachios when compared with the AA requirements for children 2 to 5 years was threonine. For DIAAS, lysine was the first limiting AA in both raw and roasted pistachios when compared with the AA requirements for children 3 years and older, adolescents, and adults. Based on the DIAAS cut-off values describing protein quality (FAO, 2013), raw pistachios and roasted pistachios can both be considered a ‘Good’ quality protein if consumed by children older than 3 year, adolescents, and adults.

scholarly journals Whey protein isolate and glycomacropeptide decrease weight gain and alter body composition in male Wistar rats

2008 ◽  
Vol 100 (1) ◽  
pp. 88-93 ◽  
Author(s):  
Peter J. Royle ◽  
Graeme H. McIntosh ◽  
Peter M. Clifton
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Weight Gain ◽  
Whey Protein ◽  
Wistar Rats ◽  
Body Weight Gain ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Whey Protein Concentrate ◽  
Protein Concentrate

The effect of feed protein type on body composition and growth has been examined. Evidence exists that whey protein concentrate is effective at limiting body fat expansion. The presence of caseinomacropeptide, a mixture of glycosylated and non-glycosylated carbohydrate residues, in particular glycomacropeptide (GMP) in whey protein concentrate may be important for this effect. The influence of whey protein isolate (WPI) and GMP on weight gain and body composition was examined by feeding Wistar rats ad libitum for 7 weeks with five semi-purified American Institute of Nutrition-based diets differing in protein type: (1) casein; (2) barbequed beef; (3) control WPI (no GMP); (4) WPI+GMP at 100 g/kg; (5) WPI+GMP at 200 g/kg. Body composition was assessed, and plasma samples were assayed for TAG, insulin and glucose. Body-weight gain was lower ( − 21 %) on the control WPI diet relative to casein, with a non-significant influence associated with GMP inclusion ( − 30 %), the effect being equivalent at both levels of GMP addition. Renal and carcass fat mass were reduced in the highest GMP diet when compared with WPI (P < 0·05). Plasma insulin was lowered by GMP at the highest addition compared with WPI alone ( − 53 %; P < 0·01). Plasma TAG in the WPI+GMP (200 g/kg) group were lower ( − 27 %; P < 0·05) than the casein and beef groups. In conclusion, GMP appears to have a significant additional influence when combined with WPI on fat accumulation. WPI alone appears to have the predominant influence accounting for 70 % of the overall effect on body-weight gain. Mechanisms for this effect have not been identified but food intake was not responsible.

scholarly journals PSIX-2 Amino acid digestibility and digestible indispensable amino acid scores of a few protein isolates and concentrates derived from cereal grains, plant proteins, or dairy proteins

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 186-187
Author(s):  
Hannah M Bailey ◽  
Hans H Stein
Keyword(s):  
Amino Acid ◽  
Block Design ◽  
Brown Rice ◽  
Latin Square ◽  
Protein Isolate ◽  
Experimental Unit ◽  
Protein Concentrate ◽  
Protein Isolates ◽  
Excellent Quality ◽  
Indispensable Amino Acid

Abstract The objective of this experiment was to determine standardized ileal digestibility (SID) of amino acid (AA) and digestible indispensable amino acid scores (DIAAS) for brown rice protein concentrate (BRPC), pea protein concentrate (PPC), two rapeseed protein isolates (RSP-1 and RSP-2), soy protein isolate (SPI), and whey protein isolate (WPI). Seven cannulated barrows (36.51 ± 1.61 kg) were randomly allotted to a 7 × 7 Latin square design and fed a diet containing each protein as the sole source of AA. A nitrogen-free diet was also used. The DIAAS was calculated for children from 6-mo to 3-yr and persons &gt;3-yr. Data were analyzed using PROC MIXED of SAS in a randomized complete block design with pigs as the experimental unit. Among proteins, WPI had the greatest (P &lt; 0.05) SID of all indispensable AA (IAA), except Lys and Thr, and RSP-1 had the least (P &lt; 0.05) SID of most IAA. The SID of all AA, except Leu and Met, did not differ between RSP-2 and SPI, and the SID of all IAA, except Arg and Lys, was greater (P &lt; 0.05) for RSP-2 than for PPC and BRPC. For DIAAS (Table 1), RSP-2 was the only protein with a DIAAS greater than 100 for children 6-mo to 3-yr, and both RSP-2 and WPI had a DIAAS greater than 100 for persons &gt;3-yr. For both reference patterns, the DIAAS for RSP-2 and WPI was greater (P &lt; 0.05) than for SPI. The DIAAS for RSP-1 was less (P &lt; 0.05) than for SPI and greater (P &lt; 0.05) than for PPC, which had a DIAAS greater (P &lt; 0.05) than BRPC. In conclusion, RSP-2 resulted in an excellent quality protein for persons &gt;6-mo, and WPI is an excellent quality protein for persons &gt;3-yr.

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