Effect of supplementing palmitic acid and altering the dietary ratio of n-6:n-3 fatty acids in low-fibre diets on production responses of dairy cows

2020 ◽  
pp. 1-11
Author(s):  
E. Ghasemi ◽  
D. Golabadi ◽  
A. Piadeh
Keyword(s):  
Palmitic Acid ◽  
Dairy Cows ◽  
Feed Efficiency ◽  
Milk Fat ◽  
Protein Concentration ◽  
Latin Square ◽  
Energy Output ◽  
Energy Reserves ◽  
Negative Impacts ◽  
Body Energy

Abstract Supplementing palmitic acid (C16 : 0) in combination with modifying the dietary n-6:n-3 fatty acid (FA) ratio may benefit energy metabolism and milk responses of dairy cows. Twelve Holstein cows (70 (sd 11) days in milk) were used in a replicated 4 × 4 Latin square and allocated to four low-fibre diets (18·5 % forage neutral-detergent fibre) supplemented with no FA (CON), or 2·4 % C16 : 0-enriched supplement (PAL), 2·4 % mixture (2:1) of C16 : 0 and n-6 FA (PW6), and mixture (2:1) of C16 : 0 and n-3 FA (PW3). The dietary ratio of n-6:n-3 was increased with PW6 (10:1) and decreased with PW3 (2·8:1), whereas PAL alone made no change in the ratio (about 7:1). Compared with CON, all FA-supplemented treatments increased milk yield. However, feed and energy intakes were higher in PAL than PW3 or PW6, resulting in greater feed efficiency for PW3 and PW6 than PAL. Dietary FA supplements decreased milk protein concentration but tended to increase protein yield. Compared with CON and FA mixtures, PAL increased milk fat content and tended to increase milk SFA and atherosclerotic index. The concentration of milk n-3 FA was similar between CON and PW3. Feeding PAL increased milk energy output and decreased energy partitioning towards body reserves (−4·2 %), while this measure was positive for other treatments. Blood TAG and NEFA concentrations, but not β-hydroxybutyrate, were increased by FA-supplemented treatments. Feeding C16 : 0 combined with either n-6 or n-3 FA enhanced feed efficiency, alleviated the negative impacts on body energy reserves, but lowering the dietary n-6:n-3 ratio improved the FA profile of milk.

scholarly journals Partial replacement of soybean meal by white lupine seeds in the diet of dairy cows

2020 ◽  
Vol 33 (6) ◽  
pp. 957-964 ◽  
Author(s):  
Miroslav Joch ◽  
Václav Kudrna
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Soybean Meal ◽  
Feed Efficiency ◽  
Milk Fat ◽  
Saturated Fatty Acids ◽  
Latin Square ◽  
Partial Replacement ◽  
Plasma Metabolites ◽  
White Lupine

Objective: An experiment was conducted to determine the effect of partial replacement of soybean meal (SBM) by white lupine seeds (WLS) on milk yield and quality, feed efficiency and rumen fermentation of high-yielding dairy cows.Methods: Thirty multiparous cows of two breeds (20 Holstein and 10 Czech Pied cows) in early mid-lactation received three diets (treatments) in a 3×3 Latin square design with a 28-d period. The dietary treatments were as follows: CON (control total mixed ration with SBM, no WLS), WLS30 (30% of the SBM was replaced, on a dry matter basis, by WLS), and WLS50 (50% of the SBM was replaced by WLS).Results: Feed intake by the cows was not affected (p = 0.331) by the diets. Milk production decreased with increasing proportions of WLS in the diet. Cows fed WLS50 yielded approximately 1 kg/d (p<0.001) less milk than cows fed the CON diet. The proportions of milk fat (p = 0.640), protein (p = 0.507), and lactose (p = 0.709) were not altered by the diet. For milk fat, feeding with WLS50 reduced the proportion of total saturated fatty acids (p<0.001) and increased the proportion of total monounsaturated fatty acids (p<0.001), mainly through oleic acid (p<0.001). No differences were found in feed efficiency, body weight, and blood plasma metabolites between groups. Rumen ammonia-N levels tended (p = 0.087) to increase with increasing proportions of WLS in the diet, whereas no effect of diet on rumen pH was found (p = 0.558).Conclusion: We did not identify the safe range within which raw WLS can efficiently replace SBM in the diet of high-producing dairy cows. In contrast, even partial replacement of SBM by WLS favorably changed the milk fatty acid profile.

scholarly journals Metabolic and Productive Response and Grazing Behavior of Lactating Dairy Cows Supplemented with High Moisture Maize or Cracked Wheat Grazing at Two Herbage Allowances in Spring

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 919
Author(s):  
Verónica M. Merino ◽  
Lorena Leichtle ◽  
Oscar A. Balocchi ◽  
Francisco Lanuza ◽  
Julián Parga ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Dry Matter ◽  
Body Condition Score ◽  
Latin Square ◽  
Ruminal Fermentation ◽  
High Moisture ◽  
Plasma Urea ◽  
Grazing Behavior

The aim was to determine the effect of the herbage allowance (HA) and supplement type (ST) on dry matter intake (DMI), milk production and composition, grazing behavior, rumen function, and blood metabolites of grazing dairy cows in the spring season. Experiment I: 64 Holstein Friesian dairy cows were distributed in a factorial design that tested two levels of daily HA (20 and 30 kg of dry matter (DM) per cow) and two ST (high moisture maize (HMM) and cracked wheat (CW)) distributed in two daily rations (3.5 kg DM/cow/day). Experiment II: four mid-lactation rumen cannulated cows, supplemented with either HMM or CW and managed with the two HAs, were distributed in a Latin square design of 4 × 4, for four 14-d periods to assess ruminal fermentation parameters. HA had no effect on milk production (averaging 23.6 kg/day) or milk fat and protein production (823 g/day and 800 g/day, respectively). Cows supplemented with CW had greater protein concentration (+1.2 g/kg). Herbage DMI averaged 14.17 kg DM/cow.day and total DMI averaged 17.67 kg DM/cow.day and did not differ between treatments. Grazing behavior activities (grazing, rumination, and idling times) and body condition score (BCS) were not affected by HA or ST. Milk and plasma urea concentration increased under the high HA (+0.68 mmol/L and +0.90 mmol/L, respectively). Cows supplemented with HMM had lower milk and plasma urea concentrations (0.72 mmol/L and 0.76 mmol/L less, respectively) and tended (p = 0.054) to have higher plasma β-hydroxybutyrate. Ruminal parameters did not differ between treatments.

The effects on milk yield and composition of incorporating lactose into the diet of dairy cows given protected fat

1996 ◽  
Vol 62 (1) ◽  
pp. 1-3 ◽  
Author(s):  
P. C. Garnsworthy
Keyword(s):  
Sugar Beet ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Protein Concentration ◽  
Milk Protein ◽  
Basal Diet ◽  
Fat Supplementation ◽  
Holstein Friesian ◽  
Group A

AbstractTwenty-eight Holstein/Friesian dairy cows were divided into four groups of seven. From weeks 4 to 15 of lactation they were given a basal diet consisting of 8 kg hay, 2 kg sugar-beet feed and 2 kg grass nuts, together with a concentrate allowance of 8 kg/day. Concentrates for group A were based on cereals and soya (control). Concentrate B contained 60 g protected fat supplement per kg; concentrate C contained 100 g lactose per kg; concentrate D contained 60 g fat supplement and 100 g lactose per kg. Milk yields were 24·6, 27·7, 25·6 and 26·5 kg/day and milk protein concentrations were 32·3, 30·7, 32·7 and 31·9 g/kg for groups A, B, C and D respectively. The effect of fat supplementation on milk yield and protein concentration was significant (P < 0·05) but the effect of lactose was not significant. Milk fat concentration was not significantly affected by treatment. It is concluded that lactose can partially alleviate the depression in milk protein concentration often observed when cows are given protected fat.

scholarly journals Determination of organic components of milk as method for evaluating energy status of dairy cows

2010 ◽  
Vol 64 (1-2) ◽  
pp. 21-32
Author(s):  
Djordje Savic ◽  
D. Matarugic ◽  
N. Delic ◽  
D. Kasagic ◽  
M. Stojanovic
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Protein Concentration ◽  
High Yield ◽  
Energy Status ◽  
Milk Samples ◽  
Organic Components ◽  
Physiological Values ◽  
The Individual

The objective of the investigations described in this work was to determine the energy status and to make recommendations for correcting the cow diet at a farm of high-yield dairy cows, on the grounds of values for the concentration of organic components of milk and their ratios in individual milk samples. A total of 147 cows were examined, including 97 in the first and 50 in the second lactation. Average concentrations of milk fat and urea were within the physiological values. Namely, the milk fat concentration in cows in the first lactation was 38.88?5.07 g/l, and it was 36.47?4.82 g/l in cows in the second lactation, while the urea concentration in cows in the first lactation 3.16?0.58 mmol/l and it was 3.72?0.64 mmol/l in cows in the second lactation. The protein concentration in both groups of cows was below the physiological values, being 30.33?2.35 g/l in cows in the first lactation and 30.17?2.27 g/l in cows in the second lactation. Based on the ratio of urea and protein concentrations, as well as of fat and proteins in the individual milk samples, it was concluded that in most examined cows, both in those in the first and those in the second lactation, there is a deficit of energy, along with a deficit or relative surplus of proteins. On the grounds of the obtained results, recommendations were given for correcting the feed rations in the coming period. .

The effect of genetic merit and concentrate proportion in the diet on nutrient utilization by lactating dairy cows

1999 ◽  
Vol 132 (4) ◽  
pp. 483-490 ◽  
Author(s):  
C. P. FERRIS ◽  
F. J. GORDON ◽  
D. C. PATTERSON ◽  
M. G. PORTER ◽  
T. YAN
Keyword(s):  
Dairy Cows ◽  
Energy Intake ◽  
Milk Production ◽  
Milk Fat ◽  
Nutrient Utilization ◽  
Metabolizable Energy ◽  
Energy Utilization ◽  
Energy Output ◽  
Genetic Merit ◽  
Gross Energy

Sixty Holstein/Friesian dairy cows, 28 of high genetic merit and 32 of medium genetic merit, were used in a continuous design, 2 (cow genotypes)×4 (concentrate proportion in diet) factorial experiment. High and medium merit animals had Predicted Transmitting Abilities for milk fat plus protein yield, calculated using 1995 as the base year (PTA95 fat plus protein), of 43·3 kg and 1·0 kg respectively. Concentrate proportions in the diet were 0·37, 0·48, 0·59 and 0·70 of total dry matter (DM), with the remainder of the diet being grass silage. During this milk production trial, 24 of these animals, 12 from each genetic merit, representing three animals from each concentrate treatment, were subject to ration digestibility, and nitrogen and energy utilization studies. In addition, the efficiency of energy utilization during the milk production trial was calculated.There were no genotype×concentrate level interactions for any of the variables measured (P>0·05). Neither genetic merit nor concentrate proportion in the diet influenced the digestibility of either the DM or energy components of the ration (P>0·05). When expressed as a proportion of nitrogen intake, medium merit cows exhibited a higher urinary nitrogen output and a lower milk nitrogen output than the high merit cows. Methane energy output, when expressed as a proportion of gross energy intake, was higher for the medium than high merit cows (P<0·05), while urinary energy output tended to decrease with increasing proportion of concentrate in the diet (P<0·05). In the calorimetric studies, neither heat energy production, milk energy output and energy retained, when expressed as a proportion of metabolizable energy intake, nor the efficiency of lactation (kl), were affected by either cow genotype or concentrate proportion in the diet (P>0·05). However when kl was calculated using the production data from the milk production trial the high merit cows were found to have significantly higher kl values than the medium merit cows (0·64 v. 0·59, P<0·05) while k l tended to fall with increasing proportion of concentrate in the ration (P<0·05). However in view of the many assumptions which were used in these latter calculations, a cautious interpretation is required.

scholarly journals Fatty acids and composition of their important groups in milk fat of Czech Pied cattle

2011 ◽  
Vol 51 (No. 5) ◽  
pp. 181-188 ◽  
Author(s):  
M. Pešek ◽  
E. Samková ◽  
J. Špička
Keyword(s):  
Fatty Acids ◽  
Palmitic Acid ◽  
Dairy Cows ◽  
Milk Fat ◽  
Chromatographic Method ◽  
Monounsaturated Fatty Acids ◽  
Fat Composition ◽  
Total Proportion ◽  
The Individual ◽  
Gas Chromatographic Method

In 2003, 2004 and 2005 milk fat composition was determined three times in 55 dairy cows of Czech Pied cattle housed in a byre with stanchions and fed under conditions usual on Czech production farms. Fatty acids were determined by a gas chromatographic method, 26 acids out of the total 37 acids observed in chromatograms were identified. The highest proportions were observed for palmitic acid (29.25 &plusmn; 2.98%), oleic acid (24.47 &plusmn; 3.27%), myristic acid (12.14 &plusmn; 1.80%) and stearic acid (8.91 &plusmn; 2.44%). The proportions of saturated, unsaturated and monounsaturated fatty acids were 64.71 &plusmn; 4.18, 31.96 &plusmn; 4.20 and 27.45 &plusmn; 3.42% of total acids, respectively. The total proportion of nutritionally undesirable lauric, myristic and palmitic acid was 45.26 &plusmn; 4.77%, while that of the desirable group of polyunsaturated fatty acids was 4.51 &plusmn; 1.09%. The observed relatively wide ranges of the individual groups of fatty acids indicate that it is possible to improve the milk fat composition in Czech Pied cows. &nbsp;

Characterization of Na+, K+ ATPase in erythrocytes of dairy cows: Effect of diet and genetic selection for milk solids

1994 ◽  
Vol 74 (4) ◽  
pp. 641-647
Author(s):  
N. D. Scollan ◽  
X. Zhao ◽  
J. R. Brisbane ◽  
B. W. McBride ◽  
N. S. Jessop ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Genetic Selection ◽  
Dissociation Rate ◽  
Energy Output ◽  
Genetic Line ◽  
Ouabain Binding ◽  
Single Class ◽  
Dissociation Rate Constants ◽  
High Forage

The number and activity of Na+, K+ ATPase units were assessed with 3H-ouabain binding and 86Rb+ uptake methods, respectively, in erythrocytes from second-parity Holstein–Friesian cows at weeks 10 and 24 of lactation. The cows were either from S or C genetic lines for yield of milk fat and protein and were offered diets of either H or L forage content. The Scatchard method of analysis indicated that 3H-ouabain binding was compatible with a single class of receptors of high affinity. S-line cows had higher milk yields (P < 0.001) than C-line animals at both sampling points. Those animals fed the low-forage feed had higher levels of dry matter intake (P < 0.001) and milk yields (P < 0.001) at weeks 10 and 24 than contemporaries fed the high-forage feed. Main effects of genetic line and diet were not significant for dissociation-rate constants (Kd) and the number and activity of Na+, K+ ATPase units. The number of enzyme units was higher at week 24 than at week 10 (P < 0.1), and between-cow variation was significant for the number of enzyme units (P < 0.02). A significant genetic line × diet interaction for enzyme activity at weeks 10 and 24 was detected (P < 0.10). This was particularly pertinent at week 10, when activity was greatest for groups CH and SL and least for groups CL and SH. The reduction in ouabain-sensitive 86Rb+ uptake for groups CL and SH may reflect an imbalance between genotype and nutrient provision. Positive relationships were found between the number and activity of Na+, K+ ATPase units and the milk energy output (P < 0.1 and P < 0.01, respectively). This may reflect the increased demand in Na+, K+ pumping that is required for enhanced milk productivity and suggests that the activity of erythrocyte Na+, K+ ATPase reflects the activity of this enzyme in other tissues during lactation. Key words: Dairy cows, genotype, diet, erythrocyte, Na+, K+ ATPase

scholarly journals Effect of supplemental rumen-protected lysine, methionine or both added to diet of lactating dairy cows on milk fatty acids profile

2010 ◽  
Vol 58 (1) ◽  
pp. 87-94 ◽  
Author(s):  
Ludmila Křížová ◽  
Jiří Třináctý ◽  
Jarmila Svobodová ◽  
Michal Richter ◽  
Vladimír Černý ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Fat ◽  
Latin Square ◽  
Total Content ◽  
Experimental Period ◽  
Maize Silage ◽  
Milk Fatty Acids ◽  
Fatty Acids Profile ◽  
Lactating Dairy Cows

The objective of this study was to determine the effect of supplemental lysine (Lys), methionine (Met) or both added to diet of dairy cows in the form of rumen-protected (RP) tablets on changes in milk fatty acids (FA) profile. The trial was carried out on four lactating Holstein cows in the form of Latin square design and was divided into 4 periods of 14 d (10-d preliminary period and a 4-d experimental period). The four treatments were as follows: C – control without amino acids (AA) supplementation, L – supplement of RP Lys, M – supplement of RP Met and ML – supplement of RP Met and Lys. Cows were fed on a diet based on maize silage, lucerne hay and supplemental mixture. Milk yield in ML (34.18 kg/d) was higher than in L or M (32.46 kg and 32.13 kg, respectively, P < 0.05) and tended to be higher than in C (33.33 kg/d, P > 0.05). Protein yield in ML (1054 g / d) was higher than that found in C, L or M (990, 998 or 968 g / d, respectively, P < 0.05). Milk fat content and yield in C and ML was higher in comparison to L and M (P < 0.05). Content of short-chain FA (C 4:0–C 12:0) was not affected by the treatment except of L that was lower than in C (P < 0.05). Content of medium-chain FA in M was lower compared to C, L or ML (P < 0.05). The content of long-chain FA in M was significantly higher than in other groups (P < 0.05). The total content of SFA in M was lower than in C or ML (P < 0.05) and tended to be lower than in L. Contents of UFA, MUFA and PUFA in M were higher than in C and ML (P < 0.05).

scholarly journals Milk fatty acid composition in Holstein x Gyr dairy cows fed chopped elephantgrass-based diets containing two types of sunflower oil associated with two methods of concentrate feeding

2020 ◽  
Vol 41 (6) ◽  
pp. 2759-2778
Author(s):  
Fernando César Ferraz Lopes ◽  
◽  
Carlos Gustavo Santos Ribeiro ◽  
Norberto Mario Rodriguez ◽  
Marco Antônio Sundfeld da Gama ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Palmitic Acid ◽  
Sunflower Oil ◽  
Milk Fat ◽  
Nutrient Intake ◽  
Block Design ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
High Oleic

wo experiments were carried out in a 2 x 2 factorial arrangement with the objective of evaluating two methods of concentrate feeding for Holstein x Gyr cows fed 600 g kg-1 chopped elephantgrass-based diets supplemented at 45 g kg-1 DM with two types of sunflower oil (SO). The types of SO differed in the levels of oleic and linoleic fatty acids (FAs): high oleic/low linoleic acid – HO (73 and 10 g 100 g-1 FA, respectively) and medium oleic/medium linoleic acid – MO (43 and 34 g 100 g-1 FA, respectively). The concentrates containing HO SO or MO SO were supplied separately from the forage twice a day after the two milkings (TAD) or as part of a total mixed ration (TMR). In Experiment 1, a 4 x 4 Latin square design was used to evaluate the ruminal fermentation and degradation parameters in four rumen-cannulated cows (430±39 kg; 79±20 days in milk; 16.4±3.1 kg day-1 of milk). In Experiment 2, a randomized block design was used to evaluate the nutrient intake, plasma contents of metabolites and FAs, milk yield and composition, and FA profile of milk fat in 32 cows (444±84 kg; 75±31 days in milk; 15.4±4.8 kg day-1 of milk). The results were analyzed by mixed models (P ? 0.05). The TMR diets promoted higher nutrient intake and rumen fermentation (higher ammonia N, acetate, propionate and total volatile FA contents) without affecting milk, fat, protein and lactose yields. TAD-fed cows presented higher feed efficiency and produced milk fat with a more nutritionally desirable FA composition, with higher vaccenic and rumenic acid contents and lower trans-10 C18:1 and palmitic acid contents. The DM intake, parameters of rumen fermentation and milk, fat, protein and lactose yields were similar for the HO SO and MO SO diets. The most nutritionally positive characteristics for human health in the milk fat of HO SO-fed cows were the higher eicosapentaenoic (+34%) and oleic acid (+11%) contents and lower palmitic acid content (-10%). Higher contents of vaccenic (+71%) and rumenic (+74%) acids and lower trans-10 C18:1 (-10%), elaidic (-32%), lauric (-14%) and myristic (-11%) acid contents were the most positive aspects of the milk fat of MO SO-fed cows. Considering the magnitudes of the differences in the levels of these FAs, it is concluded that the milk fat of cows fed MO SO showed a healthier milk FA profile.

scholarly journals The effect of fat supplementation of concentrates on digestion and utilization of energy by productive dairy cows.

1981 ◽  
Vol 29 (2) ◽  
pp. 79-92 ◽  
Author(s):  
Y. van der Honing ◽  
B.J. Wieman ◽  
A. Steg ◽  
B. van Donselaar
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Crude Protein ◽  
Latin Square ◽  
Soya Bean ◽  
Metabolizable Energy ◽  
Simultaneous Reduction ◽  
Gross Energy ◽  
The One ◽  
Lipid Supplement

Two energy balance experiments each used 6 high-yielding dairy cows. In the first, a 3 X 3 Latin square with one replicate, rations were of 7 kg hay and 11 to 14 kg of concentrates having crude protein 251 g and gross energy 17.9 MJ/kg, given alone or with 5% tallow or 5% soya bean oil. Three weeks of adaptation were followed by 10 to 12 days when excreta were collected and gas exchange estimated. Digestibility and metabolizable energy of concentrates and hay were also assessed using wethers fed to maintenance only. Intakes of DM and digestibility of nutrients other than lipid did not differ among treatments with either sheep or cows. There were no apparent differences in rumen fermentation in the one cannulated cow used, and energy loss in urine was also unaffected. Lipid supplement reduced methane loss. Milk yield and milk energy were increased with tallow, with a simultaneous reduction of milk fat and percentage of protein. Soya bean oil reduced the percentage of milk fat and milk energy produced. The second trial, using similar animals and with similar management had a change-over design in which the concentrate was given alone or with 7% tallow. Results confirmed those of trial 1. The effect of level of feeding on the efficiency of utilization of energy is discussed. (Abstract retrieved from CAB Abstracts by CABI’s permission)

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