EARLY INDUCTION OF SPROUTING ON SEED TUBERS OF YAM (Dioscorea spp.) SOON AFTER TUBER INITIATION IN A HYDROPONICS SYSTEM

2015 ◽  
Vol 52 (3) ◽  
pp. 405-417 ◽  
Author(s):  
E. AWOLOGBI ◽  
E. I. HAMADINA

SUMMARYProlonged inability of yam tubers to sprout/germinate is associated with the expression of tuber dormancy, and the start of dormancy in tubers is linked to the onset of tuber formation. Although the plant hormone, abscisic acid (ABA) is known to regulate the onset and maintenance of dormancy in tubers, there are no known method(s) of inducing shoot growth (sprouting) on seed yam tubers that are harvested at 240 days after vine emergence or earlier. Therefore, this study was conducted to: (1) determine whether the absorption of fluridone, a reputed ABA inhibitor, prior to or during early seed yam tuber formation would induce sprouting, and (2) determine the effect of fluridone on early shoot growth. The study was a 2×4 factorial experiment arranged in a Completely Randomized Design (CRD) with eight treatment combinations replicated three times. Two species of yam with long dormancy durations were used: D. rotundata var TDr 131 and D. alata var TDa 98/01166; and three concentrations of fluridone were tested (30, 50, 100 µM). Young plants (69 days after vine emergence) of TDr 131 and TDa 98/01166 were grown in a coco coir medium hydroponics system using Hoagland's Nutrient Solution, with or without the test fluridone concentrations. In all fluridone treatments, most leaves of both species of yam had 30–90% of their surface bleached while the stems appeared purplish. In both Controls, all the newly formed seed tubers that were harvested at 90 and 104 days after vine emergence were dormant (devoid of any new shoots/sprouts). In contrast, over 70% of the seed tubers that developed in 30 and 100 µM fluridone had at least one new shoot, particularly in TDa 98/01166. The fluridone treatments did not significantly affect tuber weight or vegetative growth parameters like dimensions and number of leaves. This study has shown that the duration from vine emergence of the old tuber to sprouting of the new tuber can be reduced by more than half if young plants absorbed fluridone during early tuber development.

2019 ◽  
Vol 5 (2) ◽  
pp. 67
Author(s):  
Mardiana Mardiana ◽  
Zainuddin Zainuddin ◽  
Mahfudz Mahfudz ◽  
Hawalina Hawalina

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.


2019 ◽  
Vol 37 ◽  
Author(s):  
D.R.O. SILVA ◽  
A.C.M. AGUIAR ◽  
D.P. GHELLER ◽  
B.D. NOVELLO ◽  
C.J. BASSO

ABSTRACT: The objective of this study was to evaluate the initial competitive ability of canola hybrids against Brassicaceae and Poaceae species in terms of root and shoot resources. Two experiments were conducted in greenhouse, in a completely randomized design with four replications. The treatments consisted of interference conditions by root + shoot interference (root+shoot) and shoot interference (Light); and Brassicaceae and Poaceae weeds competing with hybrid of canola (Hyola 61 and Diamond) with different traits for competitiveness. The plant height, root length, dry matter of shoot and root, number of leaves and leaf area were evaluated. Data were compared using orthogonal contrasts. The turnip and volunteer canola reduced root development of the Hyola 61 in root + shoot interference, and among the Brassicaceas weeds, turnip caused greater losses. Brassicaceas weeds caused largest reductions in Diamond’s hybrid growth compared to Poaceae, mainly under light interference. Brassicaceas and Poaceae weeds cause similar damage to Hyola 61 hybrid, and the injury is higher for root +shoot interference. The black oat induce shoot growth in the Diamond hybrid under shoot interference, while among the Brassicaceae weed species, there were no differences.


Land Science ◽  
2020 ◽  
Vol 2 (1) ◽  
pp. p13
Author(s):  
Kayode Moses Babatunde ◽  
Kabiru Alani Shittu ◽  
Olusogo Adeyemi Adekanmbi ◽  
Mukaila Adetunji Asimi

Heat, moisture and oxygen are important factors that has controlled seed germination, thus A 2 ×4 factorial greenhouse experiment was conducted to determine the influence of colour and thickness of nylon mulch on soil temperature, moisture, percent germination and selected growth indicators of cucumber (Cucumis sativus L.) seedlings in a completely randomized design (CRD), where the treatments; nylon colour (white and black) and thicknesses (0, 1.5, 3.0, and 4.5 mm) were replicated three times. The analysis of variance showed that the white nylon thickness had positive significant (P ≤ 0.05) influence on soil temperature, soil moisture, plant height and number of leaves, while black nylon thickness had positive significant (P ≤ 0.05) influence on soil moisture and plant height and a negative significant (P ≤ 0.01) influence on percent germination, it was also discovered that 1.5 mm thickness of the white nylon contributed significantly (p ≤ 0.05) to the parameters measured, therefore, white nylon of 1.5 mm thickness could be recommended as mulch material for cucumber production in a sandy clay soil.


2021 ◽  
Vol 21 (1) ◽  
pp. 288
Author(s):  
Ifla Afifilah ◽  
Nunik Cokrowati ◽  
Nanda Diniarti

Sargassum sp. is a brown alga containing alginate and iodine and is used in the food, pharmaceutical, cosmetic and textile industries. Cultivation of Sargassum sp. has not been carried out, but it needs efforts to be cultivated so that its availability is sustainable. This study aims to determine the growth of Sargassum sp. cultivated with different seed weight in the longline method. This research was conducted from December 2020 to January 2021, in the waters of Ekas Bay, Ekas Buana Village, Jerowaru District, East Lombok Regency. This research was conducted using experimental methods and using the weight difference treatment of the seeds of Sargassum sp. Cultivation was carried out for 30 days. The experimental design used in this study was a completely randomized design consisting of 5 treatments and 4 replications. The treatments were treatment A (seeds weighing 25 g), B (seeds weighing 50 g), C (seeds weighing 100 g as a control), D (seeds weighing 150 g) and E (seeds with a weight of 150 g). weight 200 g). The results showed that E treatment showed the best growth results with the growth parameters measured were absolute growth, specific growth, number of leaves and number of fruits. This study concludes that different seed weight has a significant effect on the growth of seaweed Sargassum sp. with the longline method with the best seed weight in this study was the E treatment (200 g) with an absolute growth of 437.5 g, a specific growth rate of 21.50%, 4999 fruit numbers, and 3614 leaves.


2020 ◽  
Vol 16 (1) ◽  
pp. 49-62
Author(s):  
محي الدين جمعة عبد الله

The effect of adding different sugar concentrations of five treatments (T1, T2, T3, T4, T5) on rose growth CV Sarah in vitro was carried out in the Sudan University Laboratories. Data were collected for plant height, number of branches, number of leaves, and fresh weight. Statistical analysis was conducted using a completely randomized design, to find the least significant difference to compare between treatment means for MS (Murashige and Skoog) medium. Data analysis showed that the application of sugar at concentrations of 15, 30, and 45gms gave the highest means for the different growth parameters and fresh weight at a significant level, whereas the addition of sugar at 60gms concentration and sugar-free gave no significant increases. This showed clearly that addition of sugar was essential for plant growth, greater concentrations gaviz 60gm negative results, Addition of sugar at the above-mentioned concentration from 15mg,45gm to parameters studied the growth media in vitro gave a positive increase in rose growth.


2016 ◽  
Vol 3 (4) ◽  
Author(s):  
NILANTHI DAHANAYAKE ◽  
DDPBD DEHIGASPITIYA ◽  
AG MAYURI DHANANJANIE ◽  
AGKMWS ATAPATTU ◽  
PCD PERERA

The study investigated that the effect of sulfur solutions as foliar spray with different concentrations on some selected growth parameters of ladies fingers plants. Pot experiment was conducted with six treatments (2g/L, 4g/L, 6g/L, 8g/L and 10g/L sulfur solutions) and three replicates. For each pot,two plants were maintained throughout the experiment. After seedling emerging, sulfur solutions were sprayed in every week using OSATU sprayer (5 L), until all the leaves got wet. After 30 days physiological features (Number of leaves, leaf length andleaf width) were recorded. Pest and disease incident also recorded at the mean time. Completely Randomized Design (CRD) with five replicates was used. Statistical analysis was performed with Duncan’s multiple range test using SAS software (version 9.1.3). Result showed that the highest significant values in number of leaves (26), leaf length (21cm) and leaf width (24cm) were observed that in 10g/L solution of sulfur applied as foliar spray. With the increasing concentration of sulfur solutions the values for the selected physiological features also increased. Pest and disease incidence was significantly higher in control experiment while all the other treated plants don’t showed any physiological damage or symptom. The results revealed that the application of sulfur as a foliar spray can be effectively use in increase the net photosynthetic area and to reduce the pest and disease incidence.


2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
S. O. Dania ◽  
P. Akpansubi ◽  
O. O. Eghagara

A greenhouse experiment was conducted to investigate the effects of NPK, poultry manure, and organomineral fertilizer on the growth and nutrient concentration ofMoringa oleiferaleaves. The experimental design was completely randomized design (CRD) with four treatments replicated three times. Data collected were analysed using descriptive statistics and ANOVA atP=0.05. Growth parameters measured include number of leaves per plant, plant height (cm), and stem girth (mm). The application of poultry manure increased the height, number of leaves, and stem girth of moringa compared to the application of NPK and organomineral fertilizer while the control had the least growth. Poultry manure, NPK, and organomineral fertilizer were 66%, 62%, and 39% higher in number of leaves than the control at eight weeks after planting. The application of poultry manure significantly(P≤0.05)increased the nutrient content of moringa leaves compared to other sources of fertilizer applied. The results shows that the application of poultry manure significantly(P≤0.05)improved the growth and nutrient content of moringa; however, further field trial is suggested.


2018 ◽  
Vol 3 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Sanjay Mahato ◽  
Srijana Neupane

Biofertilizers may be a better eco-friendly option to maintain soil fertility. The study was conducted to investigate the effect of Azotobacter and Trichoderma on the vegetative growth of maize (Zea mays L.) plants. The experiment was carried out in medium sized pots, at IAAS, Lamjung (Feb 2017 - May 2017) in completely randomized design (CRD), consisting eight treatments and three replications. Treatments were namely T1 (control), T2 (Azotobacter), T3 (Trichoderma), T4 (Azotobacter + Trichoderma), T5 (NPK), T6 (Azotobacter + Trichoderma + FYM), T7 (Azotobacter + Trichoderma + FYM + NPK), T8 (FYM). Azotobacter showed a positive increase in plant height, stem girth, dry shoot weight, root length and width, and root weight while Trichoderma displayed either negative or minimal impact. Effect of FYM was lower than Azotobacter but considerably higher than Trichoderma. Trichoderma seriously inhibited the expression of Azotobacter when used together. Trichoderma even suppressed the outcome (except shoot weight) of FYM when used together. Root length was the longest in Azotobacter inoculation. The highest number of leaves was in T7 followed by Azotobacter (T2) and NPK (T5). Unlike leaf width, Azotobacter showed a negligible increase in leaves length while Trichoderma wherever present showed the negative impact. Minimum chlorophyll content was found in Azotobacter or Trichoderma after 73 days. Azotobacter treatment showed early tasseling than Trichoderma. The association of Azotobacter and Trichoderma increased the biomass. Azotobacter has significant effects on growth parameters of maize and can supplement chemical fertilizer, while Trichoderma was found to inhibit most of the growth parameters.


2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Steven T. Mensah ◽  
Edache B. Ochekwu ◽  
Uchechukwu G. Mgbedo ◽  
Miracle C. Uzoma

This study was conducted to determine the effect of NPK (15 : 15 : 15) fertilizer on some growth parameters of Punica granatum seedlings towards establishing the fertilizer amount optimal for the growth of P. granatum. Planting bags containing 23.3 kg of soil were used for planting the seeds of Punica granatum, and the soil was treated with different amounts of NPK 15 : 15 : 15 fertilizer (T1 = 0 g, T2 = 1 g, T3 = 2 g, T4 = 3 g, and T5 = 4 g) and watered daily with borehole water. The experiment was laid out in a completely randomized design with four replicates. Data were taken on the number of leaves (NOL), height of plant (HOP), and area of leaves (AOL) every three weeks for fifteen (15) weeks after planting. Results obtained using the Ochekwu Comparative Treatment Average (OCTA) trend showed that all the parameters under investigation increased significantly with an increase in fertilizer amount compared to the control. The optimum growth was observed to range between T3 and T4 for the number of leaves, height of plant, and area of leaves parameters studied. It was also observed that the increment of fertilizer amounts beyond this optimum point reduced biological yield previously obtained. Hence, for the optimum growth of P. granatum seedlings, NPK (15 : 15 : 15) fertilizer should be applied within the range of T3 and T4 or any other measurement that falls within the concentration range of T3 (0.09 g NPK kg−1 soil) and T4 (0.13 g NPK kg−1 soil) in the Niger Delta of Nigeria.


2020 ◽  
Vol 11 ◽  
pp. e3200
Author(s):  
Janderson Carmo Lima ◽  
Marilza Neves Nascimenro ◽  
Uasley Caldas Oliveira ◽  
Anacleto Ranulfo Santos ◽  
Alinsmario Leite Silva

The objective of this work was to determine the macronutrient doses that allow the highest phytomass yield and growth of basil plants. The experiment was carried out in the experimental area of ​​the Federal University of Recôncavo da Bahia, in Cruz das Almas, BA, in a greenhouse. The treatments were obtained according to the Baconian matrix statistical arrangement, in which one of the nutrients is supplied in variable amounts, while the others are kept at a referential level. Six nutrients (nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and sulfur (S)) were evaluated in three different doses, and two additional treatments, one with reference doses and another without nutrient addition, totaling 20 treatments that were arranged in a completely randomized design with five replications, totaling 100 experimental units. At 50 days after treatment distribution, the growth parameters were evaluated: Plant height, number of leaves, leaf dry mass, stem, root and leaf area. It was observed that the highest leaf dry mass accumulation was estimated with the dose of 250 mg dm³ of N giving an average of 8.01g per plant, being significantly higher when compared to all other tested elements, this parameter is extremely relevant because the leaves are characterized as the main product of this species. Phosphorus doses allowed significant results in height and leaf area, whereas K favored higher leaf emission. Positive responses were verified for nutrients Ca, Mg and S in all parameters tested at levels above those tested as reference.


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