Lactic dehydrogenase activity in buffalo (Bos-bubalis) bull semen and seminal plasma in relation to various physical characteristics

1980 ◽  
Vol 94 (1) ◽  
pp. 255-256 ◽  
Author(s):  
R. K. Tuli ◽  
Mehar Singh
Keyword(s):  
Dehydrogenase Activity ◽  
Seminal Plasma ◽  
Lactic Dehydrogenase ◽  
Physical Characteristics ◽  
Bull Semen ◽  
Ldh Activity ◽  
Buffalo Semen

In anaerobic glyoolysis lactic dehydrogenase (LDH) is the terminative enzyme in the sequence of reactions that promote the breakdown of sugars to lactate. The LDH activity of cattle semen and seminal plasma together with its relationship with various characteristics of semen have been reported (Stallcup & Hayden, 1960; Roussel & Stallcup, 1965). Such work in respect of buffalo semen is not available, although there are some reports on LDH activity of spermatozoa and seminal plasma (Singh & Sadhu, 1972; Chauhan & Srivastava, 1973). The present investigation was undertaken to determine the LDH activity of buffalo whole semen as well as of seminal plasma and to find correlations with some physical characteristics of semen.

Lactic dehydrogenase activity in buffalo semen

1985 ◽  
Vol 8 (1-2) ◽  
pp. 143-149 ◽  
Author(s):  
R.S. Gupta ◽  
R.K. Srivastava
Keyword(s):  
Dehydrogenase Activity ◽  
Lactic Dehydrogenase ◽  
Buffalo Semen

scholarly journals Brief Report: Preservation of the Lactic Dehydrogenase Activity of Platelets by Freezing in Dimethylsulfoxide and Plasma

Blood ◽  
1964 ◽  
Vol 24 (6) ◽  
pp. 761-764 ◽  
Author(s):  
PHILIP H. GEISLER ◽  
IOULIOS A. IOSSIFIDES ◽  
MARY F. EICHMAN
Keyword(s):  
Lactic Acid ◽  
Dehydrogenase Activity ◽  
Lactic Dehydrogenase ◽  
Complete Loss ◽  
Slow Freezing ◽  
Lactic Acid Dehydrogenase ◽  
Acid Dehydrogenase ◽  
Ldh Activity ◽  
Native Plasma ◽  
Freezing Storage

Abstract It is concluded that a medium composed of 15 per cent Dimethylsulfoxide and 85 per cent of a mixture of equal parts native plasma and saline effectively preserves platelet lactic acid dehydrogenase activity against slow freezing, storage for 24 hours in temperatures of liquid N2 and fast thawing. Platelet lysis results in a complete loss of LDH activity.

scholarly journals Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Maja Zakošek Pipan ◽  
Petra Zrimšek ◽  
Breda Jakovac Strajn ◽  
Katarina Pavšič Vrtač ◽  
Tanja Knific ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Quality Characteristics ◽  
Freezing And Thawing ◽  
Additional Tool ◽  
Bull Semen ◽  
Bull Sperm ◽  
Fresh Semen

ABSTRACT Background Wide variation in fertility rates is observed when using frozen bull semen, even when the bulls have met quality standards for semen production. Therefore, a simple and reliable test to assess the freezing potential of bull semen based on the analysis of fresh semen or blood would be of great value. Attention is now turning to assessment of seminal plasma components such as proteins and elements. In the present study, the concentrations of macro- and microelements in fresh bull semen plasma and in serum and their correlation with quality characteristics of fresh semen and with semen quality after freezing and thawing were determined. Ejaculates were collected from 30 mature bulls, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability and DNA fragmentation were determined on the day of collection and after freezing and thawing. The concentrations of macroelements (Na, Mg, K and Ca) and microelements (Cu, Fe, Zn and Se) were determined in the seminal plasma and serum. The semen samples were classified into satisfactory and unsatisfactory groups according to the fresh semen quality. Results Zinc and Se levels measured in serum were associated with almost all fresh and frozen-thawed semen quality characteristics, while Fe levels were associated only with acrosomal defects in fresh semen. Zinc and Fe levels in fresh seminal plasma were associated with various quality characteristics of fresh and frozen-thawed semen, while Se level in fresh seminal plasma was not associated with any of the semen quality characteristics. Conclusions Microelements were shown to be useful as biomarkers involved in the analysis of bull sperm quality and could be used as an additional tool to predict bull semen quality after freezing and thawing. Our results confirm that the analysis of Zn and Se levels in serum and Zn, Cu and Fe levels in fresh seminal plasma can provide information to discriminate between bull semen samples with spermatozoa with high or low cryotolerance.

Serum lactic dehydrogenase activity in hepatic disorders in infancy and childhood

1973 ◽  
Vol 40 (9) ◽  
pp. 305-311
Author(s):  
Ishwar Chandra ◽  
S. Inamdar ◽  
A. T. Dabke ◽  
M. A. Khan ◽  
P. S. Mathur
Keyword(s):  
Dehydrogenase Activity ◽  
Lactic Dehydrogenase ◽  
Serum Lactic Dehydrogenase ◽  
Infancy And Childhood

Study of Lactic Dehydrogenase Activity and Isoenzyme Pattern in Myasthenia Gravis

1971 ◽  
Vol 5 (4) ◽  
pp. 245-255
Author(s):  
I. Szathmáry ◽  
A. Szobor ◽  
L. Selmeci ◽  
E. Pósch
Keyword(s):  
Myasthenia Gravis ◽  
Dehydrogenase Activity ◽  
Lactic Dehydrogenase ◽  
Isoenzyme Pattern

scholarly journals Effect of Supplementation of Polyvinylpyrrolidone in Extender on Buffalo Semen Parameters

2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Bushra Ismail Khan ◽  
Shamim Akhter ◽  
Sanwal Aslam ◽  
Rabea Ejaz
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Bubalus Bubalis ◽  
Semen Parameters ◽  
Suction Pump ◽  
Bull Semen ◽  
Buffalo Semen ◽  
Live Sperm ◽  
And Control

The current study was planned to evaluate the supplementation of Polyvinylpyrrolidone in extender on cryopreservation of Nili-Ravi buffalo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concentrations of PVP (0.01, 0.05, 0.1mM). The extender without PVP was kept as control. Semen was stored at 4°C for a period of 2 h and kept at 4°C for 4h. Semen was filled in 0.5 ml French straws using suction pump at 4°C, plunged and stored in liquid nitrogen (-196°C). Semen straws were rewarmed at 37°C for 30 seconds and assessed for sperm motility, plasma membrane integrity (PMI), dead sperm percentage and the live sperm percentage. The data on the role of PVP on different parameters of semen quality were analyzed by using ANOVA and RCBD. Higher percentage (P< 0.05) of sperm motility (66.1±7.51 and 59.4±10.72) and PMI (72.9±5.39 and 75.7±6.5) was observed in extenders having 0.05 mM and 0.1mM PVP compared to extenders having 1.5mM PVP and control. The percentage acrosomal integrity was observed greater (P< 0.05) in extended semen containing 0.1mM (68.2±0.50) PVP compared to extenders having 0.01 and control.

scholarly journals Malondialdehyde Level in Seminal Plasma of Cryopreserved Holstein Bull Semen after Addition of Zinc, Cysteine, Prostaglandin F2α and their Combination in vitro

2020 ◽  
Vol 13 (1) ◽  
pp. 97-100
Author(s):  
M. R. G. Al-Dahan ◽  
A. F. Majeed ◽  
M. A. Abed ◽  
F. Ibrahim ◽  
K. J. yahya
Keyword(s):  
Seminal Plasma ◽  
Zinc Sulphate ◽  
Prostaglandin F2α ◽  
Treated Group ◽  
Control Group ◽  
Bull Semen ◽  
Holstein Bull ◽  
Holstein Bulls ◽  
Combination Treated Group

The study was conducted to know the level of Malondialdehyde (MDA) in seminal plasma of cryopreserved semen of Holstein bulls after addition of zinc sulphate, cysteine, PGF2α and their combination in vitro. Semen was collected from 7 Holstein bulls, presented in Artificial insemination Center which belonged to the Directorate of Animal Resources/ Ministry of Agriculture at Abu-Graib at the west of Baghdad. Pooled semen were diluted with Tris- based extender and divided into five parts. The first part (T1) serve as a control (without addition). The 2nd part (T2) added to it zinc sulphate (0,576 mmol/ ml). The 3rd part (T3) added to it cysteine (5 mmol/ ml). The 4th part (T4) added to it PGF2α (37.5 pg/ ml). while the 5th part added to it a combination of previous substances at the same concentration. They packed in straws and cryopreserved in a liquid nitrogen and after 30, 60 and 90 days. Seminal plasma when isolated to measure the level of MDA. The results showed a significant decrease (P>0.01) in MDA level in the combination treated group (zinc, cysteine and PGF2α) 0.450 ± 0.11 (mmol/ ml) as compared with control group 1.025 ± 0.38 (mmol/ ml), zinc 0.867 ± 0.12 (mmol/ ml), cysteine 1.06 ± 0.12 (mmol/ ml) and PGF2α group 0.968 ± 0.17 (mmol/ ml) respectively. It was concluded from this study that addition of a combination of zinc, cysteine and PGF2α to the Holstein bull semen could decrease the level of MDA which might be due to the synergistic effect of these substances.

CEREBROSPINAL FLUID LDH IN 287 CHILDREN, INCLUDING 53 CASES OF MENINGITIS OF BACTERIAL AND NON-BACTERIAL ETIOLOGY

PEDIATRICS ◽  
1968 ◽  
Vol 41 (6) ◽  
pp. 1097-1103
Author(s):  
William Neches ◽  
Martin Platt
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Dehydrogenase Activity ◽  
Newborn Infants ◽  
Central Nervous System Disease ◽  
Lactic Dehydrogenase ◽  
Spinal Fluid ◽  
Initial Examination ◽  
System Disease

Cerebrospinal fluid lactic dehydrogenase activity was determined in 287 children. Among these, 87 had no central nervous system disease and were considered to be controls. Mean lactic dehydrogenase activity in 69 controls (excluding newborn infants) was 14 units. In 18 control infants less than 1 week of age, the mean lactic dehydrogenase activity was 50 units. Thirty-two patients with bacterial meningitis had a mean cerebrospinal fluid lactic dehydrogenase of 251 units on the initial examination; 20 patients with aseptic meningoencephalitis had a mean lactic dehydrogenase activity of 23 units. The difference between the lactic dehydrogenase activity in children with bacterial and aseptic meningitis was highly significant (p &lt; 0.005). The clinical course of the patients studied was reflected by the change in cerebrospinal fluid lactic dehydrogenase activity on serial determinations. Spinal fluid isoenzyme patterns were studied in a few patients with bacterial and non-bacterial central nervous system disorders. This study indicates that the determination of lactic dehydrogenase in spinal fluid is a useful adjunct to other cerebrospinal fluid parameters in the differential diagnosis of central nervous system infections.

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