The effect of implantation of anabolic steroids into suckling and ruminating lambs on the metabolism of alanine in livers perfused in the presence or absence of volatile fatty acids

SUMMARYThe effect of implantation of trenbolone acetate and oestradiol into lambs soon after birth on the metabolism of alanine in the presence and absence of propionate has been studied in isolated perfused livers obtained from suckling and from ruminating lambs. The effect of butyrate on the metabolism of alanine in the presence and absence of propionate has been studied in livers obtained from the steroid implanted lambs.Glucose production in the presence of alanine by livers from non-implanted suckling lambs was more than 3 times greater than that by livers from non-implanted ruminating lambs. Steroid implantation did not affect alanine uptake by the livers but metabolism within the livers was altered. Significant interactions were observed between the age of the lambs and whether or not they had been implanted with steroids for the rates of net glucose production in the absence of propionate (P < 0·01) and for the rates of release of urea by the livers in the presence of (P < 0·05) and absence of propionate (P < 0–01).Propionate did not affect alanine uptake by livers obtained from either suckling or ruminating lambs, but there was a significant interaction (P < 0·05) between the age of the lambs and whether or not butyrate was infused, indicating that butyrate depressed alanine uptake by livers obtained from implanted ruminating lambs perfused in the absence of propionate. Despite this depression of alanine uptake the rates of net glucose production and urea production were unchanged, indicating that butyrate modified the metabolism of alanine in the livers of implanted ruminating lambs in that a greater proportion of the alanine carbon taken up must have been converted to glucose. Butyrate stimulated net glucose production in the presence of propionate and alanine in livers from suckling (P < 0·05) but not ruminating lambs. Butyrate was ketogenic and the release of 3-hydroxybutyrate accounted for 45 and 30% of the carbon taken up as butyrate by livers from suckling and ruminating lambs respectively perfused in the absence of propionate. Propionate was antiketogenic. Propionate inhibited urea formation from alanine by livers from implanted suckling (P < 0·05) and implanted ruminating (P < 0·001) lambs. This effect was diminished by the presence of butyrate. Lactate was released in the presence of propionate and accounted for approximately 12–5% of the carbon taken up as propionate.It may be concluded that implantation of lambs with anabolic steroids maintains hepatic gluconeogenesis from alanine at rates more closely resembling those found in younger suckling lambs. The effects of the volatile fatty acids on alanine metabolism are likely to be of physiological significance when sheep are fed diets which result in a markedly altered pattern of rumen fermentation.

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Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

Animals ◽

10.3390/ani11010108 ◽

2021 ◽

Vol 11 (1) ◽

pp. 108

Author(s):

Yichong Wang ◽

Sijiong Yu ◽

Yang Li ◽

Shuang Zhang ◽

Xiaolong Qi ◽

...

Keyword(s):

Fatty Acids ◽

Acetic Acid ◽

Propionic Acid ◽

Acid Content ◽

Volatile Fatty Acids ◽

Rumen Fermentation ◽

Gas Production ◽

Quadratic Response ◽

In Vitro Rumen Fermentation

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

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Effect of time of supplementary feeding on intake, apparent digestibility and rumen fermentation of grass hay by sheep

Animal Science ◽

10.1017/s0003356100007704 ◽

1994 ◽

Vol 59 (2) ◽

pp. 217-222 ◽

Cited By ~ 2

Author(s):

M. D. Carro ◽

A. R. Mantecón ◽

I. A. Wright ◽

I. J. Gordon

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Plasma Concentrations ◽

Rumen Fermentation ◽

Apparent Digestibility ◽

Nonesterified Fatty Acids ◽

Rumen Ph ◽

Grass Hay ◽

Forage Intake ◽

Male Sheep

AbstractEffects of time of supplementation on forage intake, nutrient apparent digestibility and rumen fermentation were studied with 12 mature castrated male sheep (wethers) offered grass hay from 16.30 h to 09.30 h and supplemented with a cereal-based concentrate given at either 09.30 or 16.00 h. Voluntary intake of hay organic matter (OM) was decreased by feeding the concentrate (P< 0·01). Offering concentrate at 09.30 h after hay was available, increased intake of hay and total OM compared with offering it at 16.00 h before hay was available (P< 0·05). Daily pattern of hay intake was not changed when concentrate was offered at 09.30 h compared with feeding hay alone, but concentrate given at 16.00 h resulted in a lower hay intake between 16.30 and 18.00 h. Sheep offered concentrate at 09.30 h had higher rumen ammonia levels than those offered concentrate at 16.00 h, but there were no differences in the apparent digestibility of the nutrients, rumen pH and molar proportions of the main volatile fatty acids. There were also no differences between groups in the blood plasma concentrations of 3-hydroxybutyrate (30HB), nonesterified fatty acids (NEFA) and glucose. It is concluded that feeding supplement after rather than before a period of intake of forage or a bout of grazing may offer a means of minimizing reduction of forage intake as a consequence of feeding concentrate.

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Concentrate containing calcium salts of fatty acids rich in polyunsaturated fatty acids can change rumen fermentation in grazing goats

Semina Ciências Agrárias ◽

10.5433/1679-0359.2018v39n6p2621 ◽

2018 ◽

Vol 39 (6) ◽

pp. 2621

Author(s):

Ludmila Couto Gomes ◽

Claudete Regina Alcalde ◽

Julio Cesar Damasceno ◽

Luiz Paulo Rigolon ◽

Ana Paula Silva Possamai ◽

...

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Rumen Fluid ◽

Latin Square ◽

Rumen Fermentation ◽

Ruminal Fermentation ◽

Calcium Salts ◽

Linear Effect ◽

Concentrate Supplementation ◽

Ruminal Ph

Feeding goats with calcium salts of fatty acids (CSFA) can supply ruminants with lipids, with minimal effects on ruminal fermentation and fiber digestibility. However, there is a shortage of information on the effect of CSFA on characteristics of rumen fermentation in grassland goats. Thus, the present study aimed to assess the addition of CSFA to concentrate on the parameters of rumen fermentation of grazing goats. Five rumen cannulated goats were distributed in a Latin square 5x5 design (treatments: 0%, 1.5%, 3.0%, 4.5% and 6.0% CSFA. The pH, ammonia N and volatile fatty acids (VFA) content were analyzed in the ruminal fluid at 0, 2, 4, 6 and 8 hours after concentrate supplementation. The pH and ammonia N concentration showed a linear effect with the addition of CSFA. There was no effect observed for the VFA molar concentration after grazing goats were fed with the experimental diet. In conclusion, further research is needed to investigate the addition of CSFA to goat diets because there is evidence that CSFA increases ruminal pH and decreases excess ruminal ammonia without changing the VFA concentration in the rumen fluid.

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Intraruminal administration ofMegasphaera elsdeniimodulated rumen fermentation profile in mid-lactation dairy cows

Journal of Dairy Research ◽

10.1017/s0022029911000707 ◽

2011 ◽

Vol 79 (1) ◽

pp. 16-25 ◽

Cited By ~ 10

Author(s):

Qendrim Zebeli ◽

Sarah J Terrill ◽

Alberto Mazzolari ◽

Suzanna M Dunn ◽

Wen Z Yang ◽

...

Keyword(s):

Fatty Acids ◽

Dairy Cows ◽

Milk Yield ◽

Volatile Fatty Acids ◽

Rumen Fluid ◽

Plasma Concentrations ◽

Rumen Fermentation ◽

Holstein Cows ◽

Plasma Metabolites ◽

Fermentation Profile

This study evaluated the effects of intraruminal administration ofMegasphaera elsdeniion ruminal fermentation patterns, the profile of plasma metabolites, and milk yield and composition of mid-lactation dairy cows. Eight primiparous, ruminally cannulated Holstein cows were arranged in a paired 2×2 crossover design. Cows were randomly assigned to one of two treatments: 1) intraruminal inoculation of 35 ml suspension per day ofM. elsdeniiATCC 25940 (MEGA), containing 108cfu/ml of bacteria, dissolved in 35 ml of saline (0·15m), or 2) carrier alone (35 ml saline; CTR). Both postprandial and preprandial rumen volatile fatty acids (VFA) and plasma metabolite measurements were analysed. Postprandial VFA patterns were affected the most, with butyrate (P<0·01) and valerate (P<0·01) proportions increasing, and acetate (P<0·01), isobutyrate (P=0·05) and isovalerate (P<0·01) decreasing in MEGA cows. Preprandial data measured at various days showed that MEGA dosage tended to increase the molar proportion of propionate (P=0·09) and lower the acetate to propionate ratio (P=0·07) in the rumen fluid. There was no effect of treatment on rumen pH and on the concentration of lactate in the rumen as well as on selected preprandial plasma metabolites. Postprandial plasma concentrations of cholesterol tended to increase (P=0·07) in MEGA cows compared with CTR. Concentrations of non-esterified fatty acids (NEFA) in the plasma were lower in MEGA cows after the morning feeding (P<0·01). Sampling hour also affected plasma NEFA in this study. Plasma β-hydroxybutyrate (BHBA) were not affected by the treatment (P>0·05); however, after the morning feeding BHBA concentration was increased in both groups of cows. Dry matter intake and milk yield and composition were not affected by treatment. In conclusion, results indicate thatM. elsdeniihas the potential to modulate the rumen fermentation profile in mid-lactation Holstein cows, but these effects were only slightly reflected in changes in plasma metabolites and milk composition.

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Further studies on the effects of dietary cottonseed oil on milk-fat secretion in the cow

Journal of Dairy Research ◽

10.1017/s0022029900019075 ◽

1968 ◽

Vol 35 (3) ◽

pp. 343-352 ◽

Cited By ~ 22

Author(s):

W. Steele ◽

J. H. Moore

Keyword(s):

Fatty Acids ◽

Milk Fat ◽

Volatile Fatty Acids ◽

Octadecenoic Acid ◽

Cottonseed Oil ◽

Rumen Fermentation ◽

Total Volatile ◽

Feeding Experiments ◽

Concentrate Mixture ◽

Low Levels

SummaryThe effects of the isocaloric replacement of part of the dietary concentrate mixture by cottonseed oil on the yield and composition of the milk fat and on the pattern of rumen fermentation was investigated in 2 feeding experiments with a total of 8 cows in mid-lactation. The concentrate mixtures were given with high- or low-roughage diets that supplied 9·1 or 1·8 kg of hay/day.In expt 1 the yield of milk fat was not altered by the addition of 10% cottonseed oil to the concentrate mixtures given either with the high- or with the low-levels of dietary roughage. On the low-roughage treatment, dietary cottonseed oil increased milk yield but reduced the fat content of the milk. The change from the high- to the low-roughage diets containing no cottonseed oil resulted in reductions in the yield and percentage of fat in the milk. In expt 2 the yields of milk and milk fat were similar irrespective of whether the concentrate mixture contained 5 or 10% cottonseed oil.In expt 1 the inclusion of 10% cottonseed oil in the concentrate mixture reduced the yields and percentages of the medium-chain fatty acids (12:0, 14:0 and 16:0) and increased the yields and percentages of the C18 fatty acids in the milk fat. In expt 2, when the concentrate mixture contained 5% cottonseed oil, the yields and percentages of all the fatty acids in the milk fat were similar to the values obtained when the concentrates contained 10% cottonseed oil. For any given concentrate mixture, the change from the high- to the low-roughage treatments in both expts 1 and 2 resulted in increases in the percentage of oleic acid in the milk fat. The highest concentration of trans-octadecenoic acid was observed in the milk fat of the cows when they were given the high-roughage diet with the concentrate mixture containing 10% cottonseed oil.In expt 2 the level of cottonseed oil in the concentrate mixture did not influence the pattern of rumen fermentation as measured by the concentrations of the various volatile fatty acids in the rumen liquor. However, the change from the high- to the low-roughage diets reduced the proportion of acetic and increased the proportions of propionic and n-valeric acids in the total volatile fatty acids in the rumen liquor.

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Essential oils effect on rumen fermentation and biohydrogenation under in vitro conditions

Czech Journal of Animal Science ◽

10.17221/7708-cjas ◽

2014 ◽

Vol 59 (No. 10) ◽

pp. 450-459 ◽

Cited By ~ 11

Author(s):

M. Gunal ◽

A. Ishlak ◽

A.A. AbuGhazaleh ◽

W. Khattab

Keyword(s):

Fatty Acids ◽

Essential Oils ◽

Dose Level ◽

Volatile Fatty Acids ◽

Rumen Fluid ◽

Rumen Fermentation ◽

Buffer Solution ◽

Cinnamon Oil ◽

Tea Tree

The effects of adding essential oils (EO) at different levels (125, 250, 500 mg/l) on rumen fermentation and biohydrogenation were examined in a rumen batch culture study. Treatments were: control without EO (CON), control with anise oil (ANO), cedar wood oil (CWO), cinnamon oil (CNO), eucalyptus oil (EUO), and tea tree oil (TEO). Essential oils, each dissolved in 1 ml of ethanol, were added to the culture flask containing 40 ml of buffer solution, 2 ml of reduction solution, 10 ml of rumen fluid, 25 mg of soybean oil, and 0.5 g of the diet. After 24 h of incubation in a water batch at 39°C, three samples were collected from each flask and analyzed for ammonia-N, volatile fatty acids (VFA), and fatty acids (FA). Expect for CNO, the proportions of acetate, propionate, and acetate to propionate ratios were not affected (P > 0.05) by EO addition. Addition of CWO, CNO, and TEO reduced total VFA concentrations (P < 0.05) regardless of dose level. The ammonia-N concentration was greater in cultures incubated with EO regardless of dose level. Compared with the CON, the concentrations of C18:0 and trans C18:1 were reduced (P < 0.05) with EO addition regardless of dose level. Compared with the CON, the concentration of linoleic acid was greater (P < 0.05) when EO were added at 500 mg/l. EO tested in this study had no effects on VFA profile but significantly reduced the formation of biohydrogenation products (C18:0 and trans C18:1).

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The effect of lipid sources on intake, rumen fermentation parameters and microbial protein synthesis in Nellore steers supplemented with glycerol

Animal Production Science ◽

10.1071/an14394 ◽

2014 ◽

Vol 54 (10) ◽

pp. 1871 ◽

Cited By ~ 2

Author(s):

Arturo Samuel Gomez Insuasti ◽

Yury Tatiana Granja Salcedo ◽

Pablo de Souza Castagnino ◽

Bruno Ramalho Vieira ◽

Euclides Braga Malheiros ◽

...

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Rumen Fluid ◽

Latin Square ◽

Ammonia Nitrogen ◽

Rumen Fermentation ◽

Ether Extract ◽

Microbial Protein ◽

Molar Proportion ◽

Fermentation Parameters

The effects of glycerol with fat sources as a feed alternative were investigated in the ration for Nellore steers (Bos indicus). Eight cannulated steers at 30 months of age with initial bodyweight of 554 ± 36.0 kg were used in a double 4 by 4 Latin square design with four consecutive 16-day periods. Treatments were three different diets with lipid sources (soybean, soybean oil and calcium salts of fatty acids) and one diet control without lipid sources. All diets formulated contained 10% crude glycerol and 5% ether extract with a forage:concentrate ratio of 35:65; corn silage was used as forage. The ruminal liquid was sampled for 24 h and ruminal fermentations were monitored by measuring pH, concentrations of ammonia nitrogenand volatile fatty acids in rumen fluid. Urine samples were obtained from the total collection for 24 h for estimation of rumen microbial protein supply using urinary purine derivatives. Our results showed that the use of lipid sources combined with glycerol did not induce significant changes in rumen pH, acetate molar proportion, ruminal microbial protein or dry matter intake. Although the acetate molar proportion was kept constant within normal parameters, the propionate molar proportion was increased by the diet containing lipid sources. Moreover, we found that there was a negative effect of lipid sources on crude protein and neutral detergent fibre corrected for ash and protein intake, and one positive effect on ether extract intake. We believe that association between fat and glycerol may affect rumen fermentation parameters through reducing fibre intake and increasing propionate production and ammonia nitrogen.

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Age-Related Changes in the Ruminal Microbiota and Their Relationship With Rumen Fermentation in Lambs

Frontiers in Microbiology ◽

10.3389/fmicb.2021.679135 ◽

2021 ◽

Vol 12 ◽

Author(s):

Xuejiao Yin ◽

Shoukun Ji ◽

Chunhui Duan ◽

Peizhi Tian ◽

Sisi Ju ◽

...

Keyword(s):

Fatty Acids ◽

Bacterial Community ◽

Growth Performance ◽

Volatile Fatty Acids ◽

Rumen Fermentation ◽

Rumen Bacteria ◽

Ruminal Fermentation ◽

Rumen Microbiota ◽

Age Related ◽

Age Related Changes

The rumen microbiota is vital for the health and growth performance of the host animal, mainly due to its role in the fermentation of ingested feed within the rumen. Attaining a better understanding of the development of the bacterial community and fermentation in the rumen can provide the theoretical basis for regulating feed utilization. This study analyzed the development of rumen bacteria in lambs from birth to 4 months of age using 16S-rRNA amplicon sequencing data and studied its relationship with ruminal fermentation. Serum levels of metabolites were monitored at 30, 60, 90, and 120 days of age, and the RandomForest approach was used to determine age-related changes in rumen bacteria. Levels of blood metabolites, ruminal fermentation, the rumen bacterial community and its functions were all affected by the age of the lambs (P < 0.05). Based on the Bray-Curtis distance within the age groups of the rumen microbiota, the similarity increased sharply after the lambs were weaned at 60 days of age (P < 0.05). The similarity between the samples collected from birth to 90 days of age and those collected at 120 days of age, increased after 20 days of age, reaching a maximum at 90 days vs. 120 days (P < 0.05). Some age-associated changes in the microbial genera were correlated with changes in the concentrations of volatile fatty acids and the levels of microbial crude protein in the rumen, including positive correlations between main volatile fatty acids and the genera of Prevotella 1, Lachnospiraceae NK3A20 group, Ruminococcus gauvreauii group, Ruminococcaceae UCG-014, and Ruminococcus 2 (P < 0.05). These results indicated that the microbial community and the function of rumen was not well-established before 20 days of age, so there is a degree of plasticity in the rumen bacterial community during the first 20 days of post-natal development in lambs, and this might provide an opportunity for interventions to improve rumen fermentation and, thus, increase their growth performance.

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Relationship between rumen odd and branched chain fatty acids and fermentation characteristics as studied in vitro

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200013107 ◽

2003 ◽

Vol 2003 ◽

pp. 151-151

Author(s):

B. Vlaeminck ◽

V. Fievez ◽

H. van Laar ◽

D. Demeyer

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Rumen Fermentation ◽

Rumen Microbes ◽

Branched Chain Fatty Acids ◽

Fermentation Pattern ◽

Branched Chain ◽

On Farm ◽

Chain Fatty Acids

Rumen microbes contain a high proportion (20 to 50%) of their fatty acids (FA) as odd and branched chain fatty acids (OBCFA; C15:0, iso C15:0, anteiso C15:0, C17:0; iso C17:0; anteiso C17:0 and C17:1) and different bacterial classes have distinctive OBCFA ‘fingerprints’. As OBCFA make up around 5% of FA in milk, it has been suggested that there is scope for these compounds to be used in on-farm diagnostic milk-based tests in relation to the rumen fermentation pattern. Correlations of milk OBCFA with rumen fermentation pattern were recently shown (Vlaeminck et al., 2002). In the current in vitro study, the potential of rumen OBCFA to predict the production of volatile fatty acids (VFA) was evaluated.

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