The effect of roughage or concentrate feeding and rumen retention time on total degradation of protein in the rumen

1979 ◽  
Vol 93 (3) ◽  
pp. 651-656 ◽  
Author(s):  
G. Ganev ◽  
E. R. Ørskov ◽  
R. Smart
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Fish Meal ◽  
Protein Supplement ◽  
Sodium Dichromate ◽  
Mathematical Expressions ◽  
Outflow Rate ◽  
Bean Meal ◽  
Soya Bean Meal

SUMMARYSamples of soya-bean meal, groundnut meal, sunflower meal and fish meal were incubated in nylon bags in the rumens of sheep receiving either whole barley or dried grass. For the vegetable sources of protein the rate of disappearance of protein was greater when they were incubated in the rumens of sheep receiving dried grass than in sheep receiving whole barley. The rate of disappearance of fish meal did not vary between sheep fed whole barley or dried grass.Rate of outflow of protein particles from the rumen was determined by rendering the protein supplement totally indigestible by a treatment with sodium dichromate. The rate of outflow was greatest with sheep receiving dried grass and increased with increasing feeding level.The mathematical expressions of degradation rate and outflow rate were combined to give the total amount of protein degraded in the rumen for the grass and barley diets at two levels of feeding.The amino acid composition of residues left in the nylon bags after 9 h of incubation were shown to be almost identical to the amino acid composition of the original protein supplement, indicating that the composition of the amino acid in the undegraded protein entering the abomasum essentially resembled that of the supplements.

scholarly journals The digestion by cattle of silage-containing diets fed at two dry matter intakes

1985 ◽  
Vol 54 (2) ◽  
pp. 483-492 ◽  
Author(s):  
H. A. Greife ◽  
J. A. Rooke ◽  
D. G. Armstrong
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Dry Matter ◽  
Soya Bean ◽  
Rumen Bacteria ◽  
Bean Meal ◽  
Soya Bean Meal

1. In a 4 x 4 Latin square experiment four cows were given, twice daily, diets consisting of (g/kg dry matter (DM)) 500 barley, 400 grass silage and 100 soya-bean meal. The diets were given at either 1.15 (L) or 2.3 (H) times maintenance energy requirements and the soya-bean meal was either untreated (U) or formaldehyde (HCH0)-treated (T).2. The passage of digesta to the duodenum was estimated using chromic oxide as a flow marker;35S was used to estimate the amount of microbial protein entering the small intestine. A microbial fraction was prepared by differential centrifugation from duodenal digesta. Samples of bacteria and of protozoa from rumen digesta were also prepared.3. The total amino acid contents of feedingstuffs, duodenal digesta, duodenal microbial material, rumen bacteria and rumen protozoa were determined by ion-exchange chromatography. The D-alanine and D-glutamic acid contents of the samples were determined by gas–liquid chromatography.4. The quantity of each amino acid entering the small intestine was significantly (P < 0,001) increased by increasing DM intake and tended to be increased by formaldehyde-treatment of the soya-bean meal. There were net losses of all amino acids across the forestomachs except for lysine, methione, o-alanine and D-glutamic acid for which there were net gains.5. There were significant (P < 0.05) differences in amino acid composition between rumen bacteria and duodenal microbial material; differences in amino acid composition between rumen bacteria and rumen protozoa were also observed.6. D-Alanine and D-glutamic acid were present in the silage but not in the barley or either of the soya-bean meals. All samples of microbes and digesta contained D-alanine and D-glutamic acid.7. The use of D-ahine and D-glUtamiC acid as markers for microbial nitrogen entering the small intestine was assessed. Estimates of the quantities of microbial N entering the small intestine based on the D-alanine or D-glutamic acid contents of rumen bacteria or duodenal microbes were significantly higher than those determined using 35S as a marker.

scholarly journals Methionine sulphoxide as a source of sulphur-containing amino acids for the young rat

1977 ◽  
Vol 37 (1) ◽  
pp. 93-105 ◽  
Author(s):  
Anne U. Gjøen ◽  
L. R. Njaa
Keyword(s):  
Amino Acid ◽  
Blood Plasma ◽  
Fish Meal ◽  
Soya Bean ◽  
Male Rats ◽  
Curvilinear Relationship ◽  
Casein Diet ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
Effect On Growth

1. Young male rats were used in five experiments to study the utilization for growth of methionine sulphoxide, and the relationship between the sulphoxide content in the diet and the level of microbiologically determined methionine activity in blood or blood plasma. In one nitrogen-balance experiment methionine and methionine sulphoxide were compared as supplements to a casein diet and a fish-meal diet.2. Methionine sulphoxide was poorly utilized for growth when tested as the sole sulphur amino acid in an amino acid diet. Substitution of one-third of the sulphoxide with cystine improved utilization so that it approached that of methionine.3. Methionine alone and in combination with methionine sulphoxide were added to a soya-bean-meal diet. The sulphoxide showed no adverse effect on growth.4. Fish meal in which methionine had been oxidized to methionine sulphoxide was tested alone and in combinations with unoxidized fish meal. Only when the oxidized meal was given alone was there an appreciable effect on growth. The fish meals used were low in cystine.5. Whereas both methionine and methionine sulphoxide improved the N balance when a casein diet was given, there was no effect when a fish-meal diet was given.6. There was a linear relationship between methionine sulphoxide content in the amino acid diets and the methionine activity in the blood plasma. Methionine sulphoxide added to a soya-bean-meal diet or present in oxidized fish meal gave a curvilinear relationship, and the observed activities were lower than with the amino acid diets. Methionine activity in blood could not be used as an indicator of moderate amounts of methionine sulphoxide in protein-containing diets.

The effect of protein source on the performance of suckling Chios ewes and Damascus goats

1988 ◽  
Vol 46 (2) ◽  
pp. 249-255 ◽  
Author(s):  
M. Hadjipanayiotou ◽  
E. Georghiades ◽  
A. Koumas
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Fish Meal ◽  
Control Diet ◽  
Soya Bean ◽  
Protein Source ◽  
Outflow Rate ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
Supplemental Protein

AbstractTwo trials, one with 36 twin-suckling Chios ewes and the other with 32 twin-suckling Damascus goats, were conducted to study the effect of protein source (fish meal v. soya-bean meal) on the pre-weaning milk yield of the dams, and the growth performance of their offspring. The control (C) concentrate mixture was similar in both trials, using soya-bean meal (SBM) (195 kg/t) as a source of supplemental protein. In the experimental diet (FME) of the ewe trial, SBM was replaced completely by fish meal (FM) whereas in the goat trial (FMG) 83 kg of SBM were replaced by 50 kg FM. Dry matter (DM) and crude protein (CP) degradation of the three isonitrogenous mixtures were measured using nylon bags incubated in the rumens (2, 6, 16, 24 and 32 h) of three Damascus goats. There were no significant differences between mixtures for DM and CP effective degradability at 005 per h outflow rate. At 0·08 per h outflow rate however, lower degradability values were obtained with the FME than with the C diet. There were differences in the effective CP degradability of SBM and FM used in the ewe trial, but the FM used in the goat trial was of higher CP degradability and similar to that of SBM. Ewes on FM produced more milk than those on the control diet (C 3·44 v. FME 3·84 kg/day), whereas milk yield of goats was similar in the two treatments (C 3·87 v. FMG 3·82 kg/day). There were no differences between treatments for ewe milk fat (C 44 v. FME 43 g/kg) or protein concentration (C 54 v. FME 53 g/kg). Goats on fish meal (FMG) produced milk of higher protein (C 37 v. FMG 40 g/kg), but of similar fat (C 42 v. FMG 43 g/kg) concentration to those on the SBM (C) diet. With the exception of the better conversion (milk: gain ratio) efficiency (C 5·17 v. FME 4·44) of male lambs sucking ewes on the FM diet, no other differences were observed in the performance of lambs or kids. Lambs consumed less milk than kids (lambs: males 4·69, females 5·13; kids: males 6·63, females 6·98) per unit of weight gain.

Effects of different protein contents in the rations of growing-fattening pigs

1960 ◽  
Vol 55 (2) ◽  
pp. 175-181 ◽  
Author(s):  
R. Braude ◽  
M. Jill Townsend ◽  
G. Harrington ◽  
J. G. Rowell
Keyword(s):  
Fish Meal ◽  
Live Weight ◽  
Soya Bean ◽  
Protein Supplement ◽  
Feed Conversion ◽  
Bean Meal ◽  
Fat Thickness ◽  
Soya Bean Meal ◽  
Fattening Pigs ◽  
Better Than

1. A co-ordinated trial has been carried out at eighteen centres to study the effects of feeding various quantities of high-protein supplement to growing-fattening pigs. In treatment 1, 10% white fish meal was included from weaning to 150 lb. live weight and 3% soya-bean meal was included from 150 lb. live weight to slaughter (approximately 210 lb.). Treatment 2 was the same as treatment 1 except that only 7% white fish meal was included during the first stage. Treatment 3 was the same as treatment 2 except that the rations were changed at 1001b. live weight instead of 1501b. Treatment 4 was the same as treatment 3 except that highprotein supplement was omitted altogether after 1501b. live weight; thus there were three rations involved in treatment 4—7% white fish meal from weaning to 100 lb. live weight, 3% soya-bean meal from 100 lb. to 150 lb. and no high-protein supplement thereafter.2. Growth rate and feed conversion both differed significantly between treatments. Pigs on treatment 1 grew 5·0% faster than those on treatment 4; the average growth rate for treatments 2 and 3, the difference between which was negligible, was 3·3% higher than for treatment 4. Feed conversion for treatment 1 was 5·4% better than for treatment 4 and the average for treatments 2 and 3 was 3·8% better than for treatment 4.3. Treatment differences were not significant for killing-out percentage, length, quality of fat, proportions of gammon, middle and fore-end of the cured side, thickness of streak, size of eye muscle and amount of fat over it. Some of the fat measurements differed significantly between treatments; differences in fat thickness at the shoulder, middle and rump between treatments 1, 2 and 3 were generally small, but the lowest protein level (treatment 4) resulted in carcasses with about 2% more fat along the back than the average of treatments 1, 2 and 3. These slight differences in back-fat thickness did not result in significant differences in the percentage of pigs in the highest grades.4. An economic appraisal of the results suggested that under the conditions prevailing at the time of the experiment treatment 3 would have resulted in the greatest profit per year, and the greatest profit per pig. This ration was relatively cheap compared with those of treatments 1 and 2 but it did not lead to a seriously impaired performance, as did that of treatment 4, the cheapest ration.

scholarly journals The effect on protein utilization of feeding different protein supplements via the rumen or via the abomasum in young growing sheep

1970 ◽  
Vol 24 (3) ◽  
pp. 803-809 ◽  
Author(s):  
E. R. Ørskov ◽  
C. Fraser ◽  
Elizabeth L. Corse
Keyword(s):  
Weight Gain ◽  
Fish Meal ◽  
Nitrogen Retention ◽  
Soya Bean ◽  
Nitrogen Excretion ◽  
Protein Supplement ◽  
Protein Supplements ◽  
Liquid Suspension ◽  
Bean Meal ◽  
Soya Bean Meal

1. Sixteen lambs were used to compare two methods of administering various protein supplements to weaned lambs. In one method the protein supplement was mixed with the dry feed, consisting mainly of rolled barley, so that it passed to the rumen. In the second method the supplement was suspended in water which the lambs were trained to suck from a bottle; the suspension then passed directly to the abomasum by way of the oesophageal groove and so escaped rumen fermentation.2. When the protein supplements were given in amounts that provided less than the estimated protein requirement of the animals, giving the liquid suspension led to significantly lower urinary nitrogen excretion (P < 0.001), greater nitrogen retention (P<0.05) and greater live-weight gain (0.05<P<0.1) than giving the feed in the dry form.3. The body-weight gain was greater with white fish meal than with casein (P<0.05) and soya-bean meal (P<0.001), whichever method of feeding was adopted. There was no significant interaction between method of feeding and protein source, but the faecal nitrogen excretion was highest when soya-bean meal was given in liquid suspension.4. From a regression of nitrogen retention on nitrogen intake with lambs receiving the basal ration only, it was calculated that the improvement in retention of the protein supplement effected by giving it in liquid suspension was 31 % for casein, 27 % for fish meal and 24 % for soya-bean meal.

The nutrition of the bacon pig: V. The minimum level of protein intake consistent with quick growth and satisfactory carcass quality

1940 ◽  
Vol 30 (1) ◽  
pp. 83-97 ◽  
Author(s):  
H. E. Woodman ◽  
R. E. Evans
Keyword(s):  
Blood Meal ◽  
Fish Meal ◽  
Live Weight ◽  
Soya Bean ◽  
Protein Supplement ◽  
Protein Food ◽  
Low Protein ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
Protein Treatment

The present investigation is the sequel to an earlier pig-feeding trial in which a diet supplying only half the standard amounts of protein supplement, in the form of a mixture of feeding blood meal, dried separated milk and ex. soya bean meal, was found to give as good results from the standpoints of rate of growth, efficiency of food conversion and carcass leanness and quality, as a diet that contained the full standard allowances.It was considered desirable to repeat this initial exploratory experiment, making use this time of a totally different form of protein supplement, since the question of the biological efficiency of the protein supply might conceivably be of critical importance at the lower levels of protein feeding. In the present trial, therefore, the mixture of the three protein foods was replaced by a single protein food, namely, white fish meal. The standard-protein treatment C supplied 12% of white fish meal up to 90 lb. live-weight, 10% from 90 to 150 lb., whilst from 150 lb. to slaughter at about 200 lb. live-weight, the white fish meal was omitted from the diet, its place being taken by 5% of ex. soya bean meal. The corresponding allowances of protein food in the low-protein treatment B were 5, 4 and 3% respectively. The low-protein treatment A, which was introduced as a link between the previous and the present trials, contained the protein supplement composed of feeding blood meal, dried separated milk and ex. soya bean meal and supplied the same amount of digestible protein as was contained in the low-protein treatment B.

The effect of increasing the proportion of barley straw in pelleted concentrate diets given to lambs on rumen outflow rate and degradation of protein supplements

1992 ◽  
Vol 54 (1) ◽  
pp. 59-66 ◽  
Author(s):  
C. Castrillo ◽  
M. Lainez ◽  
J. Gasa ◽  
J. A. Guada
Keyword(s):  
Fish Meal ◽  
Degradation Kinetics ◽  
Soya Bean ◽  
Barley Straw ◽  
Protein Supplements ◽  
Rumen Degradation ◽  
Outflow Rate ◽  
Bean Meal ◽  
Pea Seeds ◽  
Soya Bean Meal

AbstractTwo experiments are described in which growing lambs were offered ad libitum two concentrate pelleted diets containing 60 (diet C) or 230 (diet D) g/kg barley straw.In the first experiment, eight male and eight female lambs were used to determine the outflow rate from the rumen of chromium-mordanted fish meal and soya-bean meal, estimated from faecal marker excretion. Diet D promoted a higher rumen outflow rate (0·0769 per h) than diet C (0·0486 per h); no differences were found either between sexes or between Cr-mordanted protein supplements.In the second experiment, the rumen degradation of fish meal, soya-bean meal, sunflower meal, pea seeds and sweet lupin seeds was studied by incubating the supplements in polyester bags in the rumens of four lambs, following a change-over design. Increasing the proportion of straw to 230 g/kg increased the rate of nitrogen disappearance from all vegetable supplements, the differences being significant only for pea seeds.When degradation kinetics and outflow rates were considered together, effective nitrogen degradability of protein supplements was reduced by the increased proportion of dietary straw, indicating a greater influence of rumen outflow rate than of the increased rate of microbial degradation.

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