Methionine sulphoxide as a source of sulphur-containing amino acids for the young rat

1. Young male rats were used in five experiments to study the utilization for growth of methionine sulphoxide, and the relationship between the sulphoxide content in the diet and the level of microbiologically determined methionine activity in blood or blood plasma. In one nitrogen-balance experiment methionine and methionine sulphoxide were compared as supplements to a casein diet and a fish-meal diet.2. Methionine sulphoxide was poorly utilized for growth when tested as the sole sulphur amino acid in an amino acid diet. Substitution of one-third of the sulphoxide with cystine improved utilization so that it approached that of methionine.3. Methionine alone and in combination with methionine sulphoxide were added to a soya-bean-meal diet. The sulphoxide showed no adverse effect on growth.4. Fish meal in which methionine had been oxidized to methionine sulphoxide was tested alone and in combinations with unoxidized fish meal. Only when the oxidized meal was given alone was there an appreciable effect on growth. The fish meals used were low in cystine.5. Whereas both methionine and methionine sulphoxide improved the N balance when a casein diet was given, there was no effect when a fish-meal diet was given.6. There was a linear relationship between methionine sulphoxide content in the amino acid diets and the methionine activity in the blood plasma. Methionine sulphoxide added to a soya-bean-meal diet or present in oxidized fish meal gave a curvilinear relationship, and the observed activities were lower than with the amino acid diets. Methionine activity in blood could not be used as an indicator of moderate amounts of methionine sulphoxide in protein-containing diets.

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A study of the protein and amino acid requirements of the growing New Zealand White rabbit with emphasis on lysine and the sulphur-containing amino acids

British Journal Of Nutrition ◽

10.1079/bjn19780075 ◽

1978 ◽

Vol 39 (3) ◽

pp. 601-613 ◽

Cited By ~ 33

Author(s):

D. Spreadbury

Keyword(s):

Amino Acids ◽

New Zealand ◽

Amino Acid ◽

Dietary Protein ◽

Fish Meal ◽

Soya Bean ◽

N Retention ◽

Bean Meal ◽

Groundnut Meal ◽

Soya Bean Meal

1. New Zealand White (NZW) rabbits were given, between 4 and 8 weeks of age, a range of diets, based on oats and fish meal, containing from 104 to 255 g crude protein (nitrogen × 6.25; CP)/kg to establish the level of CP below which growth was retarded.2. In three experiments each diet was fed to four animals and food intake, growth and N balance were measured over 4 weeks. Body analysis was also carried out after two of the experiments.3. The rates of food intake and growth of animals increased with dietary CP concentration until a CP concentration of approximately 150 g/kg diet had been reached. Beyond this there was little further improvement. N balance studies showed that once this dietary concentration of CP had been reached, there was a reduced rate of N retention.4. Good agreement was found between N retention measured by balance methods and by body analysis: body composition showed a tendency towards an increase in fat and a decrease in N as the dietary protein concentration was reduced.5. Microbial protein produced in the caecum and eaten during coprophagy, was found to supplement the dietary protein by approximately 2 g CP/d, or by only 0.1 of a normal dietary intake of CP.6. In the second part of the study NZW rabbits were offered, between 5 and 8 weeks of age, diets based on oats containing 150 g CP/kg. The protein supplied by oats was supplemented with maize gluten, gelatin, groundnut meal, casein, soya-bean meal or fish meal.7. Rabbits offered diets containing casein, soya-bean meal and fish meal gained 40–50 g/d similar, to animals given a well-balanced control diet, while those given diets containing maize gluten, gelatin or groundnut meal gained approximately 30 g/d. This indicated that amino acid balance in dietary protein was important to the growing rabbit.8. In later experiments, diets based on cereals and groundnut meal supplemented with varying amounts of lysine and methionine were offered during a 3-week post-weaning period in order to assess requirements for those limiting amino acids.9. The addition of both lysine and methionine improved growth rates. The minimum requirements for normal growth were found to be 6.2 g methionine+cystine and 9.4 g lysine/kg diet.

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Responses of lactating ewes, offered fresh herbage indoors and when grazing, to supplements containing differing protein concentrations

Animal Production ◽

10.1017/s0003356100019012 ◽

1988 ◽

Vol 46 (3) ◽

pp. 403-415 ◽

Cited By ~ 24

Author(s):

P. D. Penning ◽

R. J. Orr ◽

T. T. Treacher

Keyword(s):

Body Condition ◽

Protein Concentration ◽

Milk Protein ◽

Fish Meal ◽

Soya Bean ◽

Protein Supplements ◽

Bean Meal ◽

Soya Bean Meal ◽

Lamb Growth ◽

Daily Milk

ABSTRACTThe responses to supplements differing in protein concentration and degradability were measured in lactating ewes and their twin lambs when offered fresh ryegrass either cut or grazed. Housed Scottish Halfbred ewes, offered fresh-cut grass ad libitum received no supplement (N) or supplements with barley and maize starch (B); barley and soya-bean meal (S); barley, soya-bean meal and fish meal (SF) or barley and fish meal (F) in weeks 2 to 7 of lactation. By feeding supplements, herbage organic-matter (OM) intake was depressed (2·00 v. 1·74 kg/day). Mean daily milk yield was increased when protein supplements were given and, because milk protein concentration was higher for supplement F and similar for all other diets, mean daily milk protein output increased with increasing fish meal in the diet. Milk yields were N 2·55, B 2·59, S 3·17, SF 3·15 and F 3·17 kg/day. Total milk solids and fat concentrations were also higher for S, SF and F than N or B. Lambs from ewes supplemented with protein grew faster and the ewes generally lost less weight and body condition compared with unsupplemented ewes.At pasture, Masham ewes grazed at herbage allowances of either 4 (L) or 10 (H) kg OM per day and received no supplement (N) or supplements B or F, for the first 6 weeks of lactation and then, in weeks 7 to 12, grazed without supplements. For NL, BL, FL, NH, BH and FH respectively lamb growth rates from birth to 6 weeks were 235, 242, 274, 267, 286 and 302 g/day; from birth to 12 weeks were 210, 209, 249, 255, 275 and 287 g/day and losses in ewe body-condition score from birth to 12 weeks were 1·28, 1·22, 1·06, 0·97, 0·62 and 0·76.It is concluded that protein supplements increased milk yield and lamb growth rates and that the response tended to be greater with fish meal.

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The digestion by cattle of silage-containing diets fed at two dry matter intakes

British Journal Of Nutrition ◽

10.1079/bjn19850133 ◽

1985 ◽

Vol 54 (2) ◽

pp. 483-492 ◽

Cited By ~ 6

Author(s):

H. A. Greife ◽

J. A. Rooke ◽

D. G. Armstrong

Keyword(s):

Small Intestine ◽

Amino Acid ◽

Glutamic Acid ◽

Amino Acid Composition ◽

Acid Composition ◽

Dry Matter ◽

Soya Bean ◽

Rumen Bacteria ◽

Bean Meal ◽

Soya Bean Meal

1. In a 4 x 4 Latin square experiment four cows were given, twice daily, diets consisting of (g/kg dry matter (DM)) 500 barley, 400 grass silage and 100 soya-bean meal. The diets were given at either 1.15 (L) or 2.3 (H) times maintenance energy requirements and the soya-bean meal was either untreated (U) or formaldehyde (HCH0)-treated (T).2. The passage of digesta to the duodenum was estimated using chromic oxide as a flow marker;35S was used to estimate the amount of microbial protein entering the small intestine. A microbial fraction was prepared by differential centrifugation from duodenal digesta. Samples of bacteria and of protozoa from rumen digesta were also prepared.3. The total amino acid contents of feedingstuffs, duodenal digesta, duodenal microbial material, rumen bacteria and rumen protozoa were determined by ion-exchange chromatography. The D-alanine and D-glutamic acid contents of the samples were determined by gas–liquid chromatography.4. The quantity of each amino acid entering the small intestine was significantly (P < 0,001) increased by increasing DM intake and tended to be increased by formaldehyde-treatment of the soya-bean meal. There were net losses of all amino acids across the forestomachs except for lysine, methione, o-alanine and D-glutamic acid for which there were net gains.5. There were significant (P < 0.05) differences in amino acid composition between rumen bacteria and duodenal microbial material; differences in amino acid composition between rumen bacteria and rumen protozoa were also observed.6. D-Alanine and D-glutamic acid were present in the silage but not in the barley or either of the soya-bean meals. All samples of microbes and digesta contained D-alanine and D-glutamic acid.7. The use of D-ahine and D-glUtamiC acid as markers for microbial nitrogen entering the small intestine was assessed. Estimates of the quantities of microbial N entering the small intestine based on the D-alanine or D-glutamic acid contents of rumen bacteria or duodenal microbes were significantly higher than those determined using 35S as a marker.

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The effect of feeding high levels of low-quality proteins to growing chickens

British Journal Of Nutrition ◽

10.1017/s0007114575000426 ◽

1975 ◽

Vol 34 (3) ◽

pp. 363-373 ◽

Cited By ~ 20

Author(s):

E. Wetnli ◽

T. R. Morris ◽

T. P. Shresta

Keyword(s):

Growth Rate ◽

Amino Acid ◽

Dietary Protein ◽

Soya Bean ◽

Maximum Efficiency ◽

Food Utilization ◽

The Third ◽

Bean Meal ◽

Groundnut Meal ◽

Soya Bean Meal

1. Three growth trials were done using male broiler chicks. In the first two trials, groundnut meal was used, with and without supplementary methionine and lysine. In the third trial, soya-bean meal was used with and without supplementary methionine. Protein levels ranged in the first trial from 120 to 420 g/kg diet and in the third trial from 120 to 300 g/kg diet. Thus the assumed minimal amino acid requirements of the chick were supplied by high levels of low-quality dietary protein.2. Diets based on cereals and groundnut meal did not support maximum live-weight gain or maximum efficiency of food utilization at any level of dietary protein. When the principal deficiencies of lysine and methionine were corrected, this protein mixture was capable of supporting the same growth rate as a control diet of cereals and herring meal.3. Diets based on maize and soya-bean meal did not support quite the same growth rate as similar diets supplemented with methionine, even though the protein level in the unsupplemented diets was sufficient to meet the assumed methionine requirements.4. These results are interpreted as examples of amino acid imbalance in diets composed of familiar feeding-stuffs. It is concluded that one cannot assume that the poor quality of a protein source can always be offset by increasing the concentration of dietary protein.

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Intensive beef production 5. The effect of different sources of protein on nitrogen retention

Animal Science ◽

10.1017/s0003356100025757 ◽

1965 ◽

Vol 7 (3) ◽

pp. 303-308 ◽

Cited By ~ 10

Author(s):

H. B. Bowers ◽

T. R. Preston ◽

N. A. MacLeod ◽

I. McDonald ◽

Euphemia B. Philip

Keyword(s):

Cottonseed Meal ◽

Dry Matter ◽

Fish Meal ◽

Nitrogen Retention ◽

Soya Bean ◽

Dry Matter Intake ◽

Voluntary Feed Intake ◽

Bean Meal ◽

Soya Bean Meal ◽

Different Sources

SUMMARY1. Nitrogen balance studies were made on 8 Friesian steers given allconcentrate diets containing fish meal, soya bean meal, groundnut meal or cottonseed meal.2. There were no differences in nitrogen retention but voluntary feed intake was significantly lower on the fish meal diet.3. Adjustment of nitrogen retention to constant dry matter intake resulted in significantly higher values for the diets containing fish meal and cottonseed meal than for the others.4. Rumen pH, rumen ammonia and blood urea were all much lower on the fish meal diet.

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Nutrition of the early-weaned calf. IX. Nitrogen retention from different protein sources in calves fed ad libitum

Animal Science ◽

10.1017/s0003356100037697 ◽

1966 ◽

Vol 8 (1) ◽

pp. 43-45 ◽

Cited By ~ 14

Author(s):

M. Kay ◽

T. R. Preston ◽

N. A. MacLeod ◽

Euphemia B. Philip

Keyword(s):

Fish Meal ◽

Nitrogen Retention ◽

Soya Bean ◽

Distillers Grains ◽

Protein Sources ◽

Dried Distillers Grains ◽

Bean Meal ◽

Ad Libitum ◽

Groundnut Meal ◽

Soya Bean Meal

1. Nitrogen balance studies were conducted on 8 early-weaned calves fed on four diets containing respectively Peruvian fish meal, soya bean meal, groundnut meal and dried distillers grains as the major sources of protein.2. Nitrogen retention differed significantly between diets, being highest on the fish meal diet, and lowest on the groundnut diet.

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Protein supplementation of silage-based diets for calves

Animal Science ◽

10.1017/s0003356100036345 ◽

1985 ◽

Vol 41 (3) ◽

pp. 293-300 ◽

Cited By ~ 14

Author(s):

R. W. J. Steen

Keyword(s):

Fish Meal ◽

Live Weight ◽

Protein Supplementation ◽

Soya Bean ◽

Vitamin Supplement ◽

Total N ◽

Concentrate Supplementation ◽

Grass Silage ◽

Bean Meal ◽

Soya Bean Meal

AbstractTwo experiments were carried out to examine the effects on the intake and performance of calves of supplementing a diet, based on high-digestibility, well-preserved grass silage, with fish meal. The effects of fish-meal supplementation were examined at each of two amounts of concentrate intake (1·05 and 2·2 kg/day) in a 2 × 2 factorial design. Fish meal was offered at a rate of 75 g/kg silage dry-matter (DM) intake. Experiments 1 and 2 involved 36 and 40 castrated male calves, respectively, which were initially 7 weeks old and had a live weight of 70 kg. The treatments were imposed for 20 weeks. The silages used in the two experiments had mean concentrations of 22-8 g nitrogen (N) per kg DM; 524 g protein N per kg total N; 59 g ammonia N per kg total N; 0·4 g butyric acid per kg DM; and 712 g digestible organic matter per kg DM. The concentrate consisted of (g/kg) 800 ground barley, 125 soya bean meal, 50 molasses and 25 mineral and vitamin supplement, respectively, and contained 29 g N per kg DM. Silage DM intakes and live-weight gains for the low and high amounts of concentrate supplementation were 1·93 and 1·46 (s.e. 0·055) kg/day and 0·84 and 1·01 (s.e. 0·013) kg/day respectively. The supplement of fish meal did not affect silage DM intake or animal performance for either concentrate intake. Proportionately, only 0·21 of the difference in live weight at the end of the treatment periods between the two amounts of concentrate intake remained at slaughter at 18 months of age. It is concluded that the optimum growth rate for autumn-born calves during their first winter can be sustained by a diet of high-digestibility grass silage supplemented with a maximum of 1·0 kg cereal-soya bean meal concentrate and that no response in performance was obtained to supplementation of this diet with fish meal.

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The effect of protein source on the performance of suckling Chios ewes and Damascus goats

Animal Science ◽

10.1017/s0003356100042318 ◽

1988 ◽

Vol 46 (2) ◽

pp. 249-255 ◽

Cited By ~ 14

Author(s):

M. Hadjipanayiotou ◽

E. Georghiades ◽

A. Koumas

Keyword(s):

Milk Yield ◽

Milk Fat ◽

Fish Meal ◽

Control Diet ◽

Soya Bean ◽

Protein Source ◽

Outflow Rate ◽

Bean Meal ◽

Soya Bean Meal ◽

Supplemental Protein

AbstractTwo trials, one with 36 twin-suckling Chios ewes and the other with 32 twin-suckling Damascus goats, were conducted to study the effect of protein source (fish meal v. soya-bean meal) on the pre-weaning milk yield of the dams, and the growth performance of their offspring. The control (C) concentrate mixture was similar in both trials, using soya-bean meal (SBM) (195 kg/t) as a source of supplemental protein. In the experimental diet (FME) of the ewe trial, SBM was replaced completely by fish meal (FM) whereas in the goat trial (FMG) 83 kg of SBM were replaced by 50 kg FM. Dry matter (DM) and crude protein (CP) degradation of the three isonitrogenous mixtures were measured using nylon bags incubated in the rumens (2, 6, 16, 24 and 32 h) of three Damascus goats. There were no significant differences between mixtures for DM and CP effective degradability at 005 per h outflow rate. At 0·08 per h outflow rate however, lower degradability values were obtained with the FME than with the C diet. There were differences in the effective CP degradability of SBM and FM used in the ewe trial, but the FM used in the goat trial was of higher CP degradability and similar to that of SBM. Ewes on FM produced more milk than those on the control diet (C 3·44 v. FME 3·84 kg/day), whereas milk yield of goats was similar in the two treatments (C 3·87 v. FMG 3·82 kg/day). There were no differences between treatments for ewe milk fat (C 44 v. FME 43 g/kg) or protein concentration (C 54 v. FME 53 g/kg). Goats on fish meal (FMG) produced milk of higher protein (C 37 v. FMG 40 g/kg), but of similar fat (C 42 v. FMG 43 g/kg) concentration to those on the SBM (C) diet. With the exception of the better conversion (milk: gain ratio) efficiency (C 5·17 v. FME 4·44) of male lambs sucking ewes on the FM diet, no other differences were observed in the performance of lambs or kids. Lambs consumed less milk than kids (lambs: males 4·69, females 5·13; kids: males 6·63, females 6·98) per unit of weight gain.

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The simultaneous use of ribonucleic acid, 35S, 2,6-diaminopimelic acid and 2-aminoethylphosphonic acid as markers of microbial nitrogen entering the duodenum of sheep

British Journal Of Nutrition ◽

10.1079/bjn19780023 ◽

1978 ◽

Vol 39 (1) ◽

pp. 165-179 ◽

Cited By ~ 114

Author(s):

J. R. Ling ◽

P. J. Buttery

Keyword(s):

Fish Meal ◽

Barley Grain ◽

Soya Bean ◽

Diaminopimelic Acid ◽

Estimation Methods ◽

Mean Values ◽

Advantages And Disadvantages ◽

Bean Meal ◽

Soya Bean Meal ◽

Microbial N

1. Three sheep, each fitted with a ruminal cannula and duodenal re-entrant cannulas were given three isonitrogenous, isoenergetic diets in a Latin-Square design. Each diet contained (/kg) approximately 400 g N as white fish meal, soya-bean meal or urea and approximately 600 g dry matter (dm) was barley grain. The diets were fed continuously and supplied about 28 g N/d.2. Total duodenal digesta was collected manually for 72 h and the proportions of microbial N in that digesta were simultaneously estimated for all sheep using RNA, radioactive sulphur (35S), diaminopimelic acid (DAPA) and aminoethylphosphonic acid (AEPA) as markers.3. Three of the estimation methods showed that the variable source of dietary N had the greatest (RNA P < 0.05, 35S P < 0.005, DAPA P < 0.1) effect on the proportions of microbial N in duodenal digesta, though differences between sheep accounted for some variation.4. These methods also ranked the diets in the order: urea > soya-bean meal > fish meal with respect to the proportions of digesta N that were microbial in origin; the respective mean values for these diets with the different markers were: RNA 0.98, 0.70, 0.56; 35S 0.92, 0.64, 0.54; DAPA 0.80, 0.47, 0.42.5. AEPA was found to be present in substantial quantities not only in isolated rumen protozoa, but also in dietary and bacterial material; an observation that invalidated its further use as a protozoal marker.6. Calculations using values obtained from the 35S procedure showed that the proportions of dietary N degraded within the rumen were 0.38, 0.43 and 0.89 for the white fish meal, soya-bean meal and barley respectively.7. The marker methods are compared and their advantages and disadvantages (real and apparent) are discussed. It is concluded that where microbial N estimates of a more general and comparative nature are required, the use of RNA as a marker is probably adequate. Where information for more exacting purposes is required, the use of 35S appears to be more appropriate.

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