Ruminal flow rate of soya-bean meal, rapeseed meal and cottonseed meal in cows fed at maintenance and at three times maintenance

1982 ◽  
Vol 98 (3) ◽  
pp. 689-691 ◽  
Author(s):  
J. E. Lindberg

The introduction of new protein evaluation systems for ruminants presupposes means to measure the ruminal protein degradability of feeds (Burroughs, Nelson & Mertens, 1975; Royet al.1977; Vérité, Journet & Jarrige, 1979). One potentially useful method to get such estimates is the nylon bag technique (Mehrez & Ørskov, 1977). This method, however, does not take into account the flow of feed particles from the rumen. By adjusting rumen degradation results obtainedin saccowith the outflow of the feed from the rumen more accurate estimates of the actual degradability is possible (0ørskov & McDonald, 1979).

1983 ◽  
Vol 101 (3) ◽  
pp. 603-612 ◽  
Author(s):  
T. Varvikko ◽  
J. E. Lindberg ◽  
J. Setälä ◽  
Liisa Syrjälä-Qvist

SUMMARYSoya-bean meal and rapeseed meal treated with 0, 0·4 or 0·8 g formaldehyde/100 g crude protein (N × 6·25) was incubated in the rumen in nylon bags with 10 and 40 μm aperture.Disappearance of dry matter and nitrogenous compounds was reduced with increasing formaldehyde treatment.The proportion of acid-pepsin soluble nitrogen in the undegraded residues of untreated and treated soya-bean meal was similar to the original samples. For rapeseed meal the proportion of acid-pepsin soluble nitrogen in the undegraded residues decreased with increasing rumen incubation time. This reduction decreased with increasing formaldehyde treatment.Concentration of amino acids in the undegraded residues did not generally deviate from those in the original samples. However, a distinguishable decrease in the methionine concentration was observed in all the rapeseed meal samples and in glutamic acid concentration in rapeseed meal treated with 0 and 0·4 g formaldehyde/100g- crude protein.Most of the changes due to rumen incubation were smaller using the 10 μm bags than using the 40 μm bags. However, the bag pore size did not play a decisive role in the conclusions of the results given.


Author(s):  
A T Chamberlain

The metabolisabie protein system (AFRC, 1992) requires the measurement of the dynamics of the degradation of protein in the rumen. However the recommended method, using fistulated animals, is slow, expensive and may be considered unacceptable by many in terms of animal welfare. The objective of this work was to develop an in vitro technique for the routine assessment of feed protein degradability.A 101 vessel fitted with an automatic stirrer was filled with modified van Soest media and rumen liquor (pooled from two sheep) and maintained under anaerobic conditions at 39.5°C. Small artificial fibre bags (4.5x3cm, pore size 54 micro-m ±4) were filled with 0.4g of soya bean meal and incubated in the vessel for 3,6,10 and 24 hours using 3 replicates at each time. Bags were washed in running cold water for 30 mins immediately after removal. Four replicates of 3g samples in large Dacron bags (10x7cm, pore size 55 micro-m ±22) were also incubated in sacco in fistulated yearling wethers using a 4x4 Latin square design to assess the base line degradability. In sacco bags were rinsed and frozen after removal and washed in a domestic washing machine at the end of the experiment.


1989 ◽  
Vol 48 (1) ◽  
pp. 157-179 ◽  
Author(s):  
S. Green ◽  
T. Kiener

ABSTRACTIn order to determine the relative digestibilities of nitrogen and amino acids in foodstuffs for pigs and poultry, and the effects of manufacturing methods, equal quantities of soya-bean meal, sunflower meals [hulled (sunflower meal 1) and dehulled (sunflower meal 2)], meat meals [made with (meat meal 1), and without (meat meal 2), blood added at 250 g/kg meat tissue (wet weights)] and rapeseed meals [seeds heated at 80°C (rapeseed meal 1) or 100°C (rapeseed meal 2)] were mixed with protein-free ingredients. The diets were given to five growing pigs with ileo-rectal anastomoses, and, by crop-intubation, to 12 caecectomized and 12 intact cocks. Excreta were collected over 48-h periods. Endogenous excretion was estimated by giving protein-free diets.In the order, soya-bean meal, sunflower meals 1, and 2, meat meals 1, and 2, rapeseed meals 1, and 2, true digestibilities were: with pigs, of nitrogen, 0·81, 0·80, 0·79, 0·64, 0·79, 0·73, 0·70 (s.e.d. 0·030), of lysine, 0·84, 0·83, 0·84, 0·65, 0·84, 0·76, 0·72 (s.e.d. 0·032); with caecectomized birds, of nitrogen, 0·92, 0·91, 0·91, 0·66, 0·78, 0·74, 0·75 (s.e.d. 0·018), of lysine 0·92, 0·91, 0·93, 0·62, 0·79, 0·70, 0·70 (s.e.d. 0·020); with intact birds, values were similar to those with caecectomized birds for soya-bean, and the sunflower meals, but lesser for meat meals 1 and 2; the solubilities of nitrogen in pepsin were 0·96, 0·92, 0·93, 0·80, 0·89, 0·87, 0·87.Two hundred and eighty pigs (initial live weights 10 kg) were used to compare growth response to free lysine with that to lysine in soya-bean meal and sunflower meal 2. Lysine availabilities, assessed by analyses of regressions of live-weight gain against lysine intake were 0·82 (s.e. 0·12) for soya-bean meal, and 0·82 (s.e. 0·18) for sunflower meal 2.Amino acid digestibilities of the sunflower meals were similar to those of soya-bean meal, and were not influenced by dehulling; values for the rapeseed meals were lower, and unaffected by differences in heating severity; values for the meat meal were reduced by blood addition. Values differed between pigs and poultry, but there was consistency in the extent to which each species discriminated between some foodstuffs. The pepsin test was insensitive. The large standard errors associated with availability values prevented meaningful comparisons with digestibility values.


1994 ◽  
Vol 72 (6) ◽  
pp. 911-922 ◽  
Author(s):  
J. Jacques Matte ◽  
Christiane L. Girard

Sixty piglets selected after weaning at 4 weeks of age were assigned to five replicates of twelve animals each. In each of these replicates the postprandial variations in serum pteroylglutamate after the ingestion of twelve sources of dietary pteroylglutamic acid were recorded twice weekly at 10 and 16 weeks of age. In six of these sources of pteroylglutamic acid the chemically pure form of the vitamin was incorporated into a semi-purified diet at concentrations varying between 0 and 1·0 mg/kg. The six other sources were provided by a soya-bean meal, rapeseed meal, maize, barley, wheat, and a commercial vitamin premix. The concentrations of pteroylglutamates measured by radioimmunoassay in the different feedstuffs were, in most cases, far from the values reported in the literature, except for maize. Indeed, while total pteroylglutamates in barley, wheat and rapeseed meal were lower by 35–56%. 17–50% and 60% respectively compared with references values, the corresponding values for soya-bean meal ranged from one third to twice as much. The area under the curve (AUC) of the pre- and postprandial (1, 2, 3, 5 and 7 h) serum pteroylglutamate following ingestion of increasing levels of chemically pure pteroylmono- glutamic acid was used to derive a regression for the 100% bioavailability of dietary pteroylglutamic acid. The corresponding AUC for the feedstuff sources of pteroylglutamates were used in the regression to determine the proportion of bioavailable pteroylglutamates out of total pteroylglutamates measured in these ingredients. No relationship (P0·66) was found between the level of chemically pure dietary pteroylmonoglutamic acid and the postprandial AUC. In fact, there was no significant (P0·11) increase in the postprandial concentration of serum pteroylglutamate for any of the pteroylglutamate sources used except for wheat. Moreover, values tended (P0·08) to be lower at 5 and 7 h postfeeding except for wheat and barley. It was hypothesized that this decrease is probably linked to the postfeeding variation in bile secretion which drains considerable amounts of circulatory pteroylglutamates. The results of the present experiment indicate that further research on analytical procedure is needed in order to provide a reliable method for measuring concentrations of pteroylglutamic acid in different sources of a given feedstuff used in pig feeding. In addition to this analytical concern, the measurement of the proportion of bioavailable pteroylglutamic acid in feedstuffs for pigs using postprandial variations of serum pteroylglutamates appears to be technically hazardous.


1987 ◽  
Vol 44 (1) ◽  
pp. 75-82 ◽  
Author(s):  
J. A. Stedman ◽  
R. Hill

ABSTRACTThe voluntary food intake in a limited period, 30 or 60 min after morning and afternoon feeds, and during 24 h, by lambs and calves given diets containing rapeseed meal (RSM) or soya bean meal (SBM) as the only protein supplement was determined. Four rapeseed meals were compared, one from seed of British-grown winter Brassica napus varieties (BRSM), and the others from seeds of the varieties Tower (TRSM) and Loras (LRSM), or from seeds of Canadian spring sown varieties (Canola; CRSM). The effects on food intake of treating BRSM with heat and ammonia, steam, steam and ammonia, or calcium hydroxide and ammonia were also determined.In lambs, intakes in a limited period of BRSM and CRSM were significantly lower than that of SBM, and there were no differences between intakes of BRSM and CRSM. In calves, intake in a limited period of BRSM was significantly lower than intake of SBM, TRSM and LRSM: those of TRSM and LRSM were lower, but not significantly so, than that of SBM. Treatment of BRSM with steam, steam and ammonia or calcium hydroxide and ammonia, increased food intake significantly but by small amounts: intakes of the treated meal diets were markedly lower than that of the control SBM diet. The glucosinolate concentration of untreated RSM influenced intake in calves but not in lambs. The effects of treatment of RSM on intake were not closely related to changes in glucosinolate concentration.


1969 ◽  
Vol 23 (1) ◽  
pp. 113-123 ◽  
Author(s):  
N. J. Drouliscos ◽  
J. P. Bowland

1. The biological evaluation of solvent and prepress-solvent processed rapeseed meals RM(S) and RM(PS) was carried out with the weanling (phase 1) and the mature (phase 2) rat. The nutritional indices measured were compared with those for diets containing soyabean meal SM and casein CS.2. The true nitrogen digestibility (td(N)) for RM(S), RM(PS), SM and CS in the weanling rat was (%) 78.9 f±1.2, 77.9±2.7, 90.4±0.9 and 96.0±0.6 (P < 0.01) respectively. Net protein utilization was (%) 68.9±10.1, 65.6±3.5, 80.6±4.8 and 81.7±3.1 (P < 0.05) respectively. Biological value was (%) 83.0±7.9, 84.1±5.6, 8.90±5.1 and 85.0±3.4 (P < 0.05) respectively. The same indices when measured in the mature rat showed an increase over those obtained in the weanling rat.3. The growth-promoting effects of the test diets were evaluated over a period of 8 weeks with ad lib. feeding between phases 1 and 2. Protein efficiency ratio averaged 2.0±0.14, 1.9±0.35, 2.4±0.39 and 2.3±0.25 for the RM(S), RM(PS), SM and CS treatments respectively.4. The daily endogenous urinary N excretion was 1.58±0.14 mg N/basal kcal or 254 mg N/kg body-weight for the weanling rat and 0.16 mg N/basal kcal or 18 mg N/kg body-weight for the mature rat. Metabolic faecal N excretion was 98.0±9.3 mg N/kg body-weight, or 27.9% of the total N output for the weanling rat, and 37.9±7.2 mg N/kg body-weight, or 67.3% of the total N output for the mature rat during the ‘protein-free’ feeding periods.5. The fresh weight of the thyroid gland per kg metabolic body size was 70, 90, 40 and 40 mg respectively for the RM(S), RM(PS), SM and CS dietary treatments.6. The moisture content of the carcasses was 66.9±3.1, 63.6±2.3 and 64.2±2.2 and the protein content on a dry-matter basis was 55.9±9.6, 50.2±6.4 and 53.1±4.9 for the RM (S and PS), SM and CS diets respectively. The results suggest an increased lean body mass in the rats receiving the rapeseed meal treatments.7. Under the conditions of this experiment solvent-extracted rapeseed meal was superior to prepress-solvent meal on the basis of rat growth response and of results for nutritional indices. Both rapeseed meals were inferior to soya-bean meal and casein.


1989 ◽  
Vol 112 (1) ◽  
pp. 79-83 ◽  
Author(s):  
D. Sklan

SummaryThe in sacco, in vitro and in vivo effects of feeding proteins partially coated with calcium soaps of longchain fatty acids were examined.In sacco, 84–90% of whey powder and soya-bean meal coated with calcium salts of fatty acids remained after 20 h incubation in the rumen of sheep. In vitro tests revealed no effects on volatile fatty acid or ammonia production.In vivo sheep balance studies, where soya-bean meal coated with calcium soaps was substituted for soya-bean meal, showed no effects on ammonia or volatile fatty acid production in the rumen due to the calcium soap coated proteins. No changes were observed in digestibilities of dry matter, nitrogen or acid detergent fibre; total fatty acid digestion increased. Nitrogen balance was improved slightly in sheep fed the protected protein.It appears that proteins coated with calcium soaps are not degraded in the rumen and thus energy and non-degradable protein can be supplied to ruminants by this route.


1987 ◽  
Vol 44 (3) ◽  
pp. 415-420 ◽  
Author(s):  
I. G. Partridge ◽  
A. G. Low ◽  
J. J. Matte

ABSTRACTIn order to determine the relative nutritional value of rapeseed meal (var. Tandem) in comparison with soya-bean meal and fish meal, seven barley-based diets were formulated to provide 13·1 MJ digestible energy and 8·8 g total lysine per kg. They contained soya-bean meal (S), rapeseed meal (R) or fish meal (F) alone, or equal proportions (on a total nitrogen basis) of soya and rapeseed, soya and fish meal, rapeseed and fish meal or soya, rapeseed and fish meal. The study involved 14 pigs growing from 40 to 90 kg, each fitted with a T cannula in the terminal ileum. Dietary treatments were applied during 7-day periods according to a 7 × 7 Latin-square design, replicated twice. The pigs were fed at 12-h intervals. Ileal digesta were collected during two 12-h periods from each pig after adaptation to each diet. Nutrient apparent digestibilities were measured using chromium III oxide as a marker; for diets S, R and F, respectively, values were: dry matter, 0·58, 0·51 and 0·66 (s.e. 0·014); N, 0·72, 0·67 and 0·73 (s.e. 0·011); lysine, 0·79, 0·72 and 0·83 (s.e. 0·010), with a similar relationship between diets for most other amino acids. In general, values for diets containing combinations of the protein supplements were intermediate between those for diets containing the respective single supplements. Following the main trial, seven pigs were given a semi-purified diet containing rapeseed meal as the only protein source; this gave amino acid digestibility values similar to or slightly higher than diet R. Diet R, which contained 375 g rapeseed meal per kg, presented no palatability problem. Rapeseed meal of the quality used in this trial could contribute a substantial proportion of the protein supplement for growing pigs. Its value should be enhanced by formulating diets according to the ileal digestibility of the limiting amino acids.


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