Effect of cactus pear as a moistening additive in the production of rehydrated corn grain silage

2022 ◽  
pp. 1-12
Author(s):  
D. M. Pereira ◽  
E. M. Santos ◽  
J. S. Oliveira ◽  
F. N. S. Santos ◽  
R. C. Lopes ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Chemical Composition ◽  
Dry Matter ◽  
Cactus Pear ◽  
Aerobic Stability ◽  
Microbiological Characteristics ◽  
Randomized Experimental Design ◽  
Four Levels ◽  
Corn Grain

Abstract The current study aimed to evaluate the effects of cactus pear as a moistening additive on fermentative and microbiological characteristics, aerobic stability (AS), chemical composition and in situ rumen degradability of corn grain silage at different opening times. A completely randomized experimental design was adopted in a 4 × 3 factorial scheme with four levels of dry matter (DM) (50; 60; 70 and 80% of DM) and three opening times (30; 60 and 120 days after ensiling), with four replications. There was an effect of interaction (P < 0.05) between the DM levels and opening times on silage yeast population, effluent losses, gas losses, dry matter recovery (DMR), AS of the silage and on lactic acid bacteria, mould and yeast populations after AS trial. The 60% DM level presented DMR values above 930 g/kg of DM. However, the lowest AS time (96.52 h) was observed in silages with 60% DM at 60 days after ensiling, although all silages have shown high AS. The DM in situ degradability of the ensiled mass increased after the ensiling process at all DM levels and opening times, with the 60% DM content showing the best result. When using cactus pear as a corn grain moistening additive, the 60% DM level is recommended when the opening time is up 120 days.

scholarly journals Effect of 11CFT and 11C33 inoculants on the chemical and fermentation composition, and aerobic stability of corn silage during the feed out period

2021 ◽  
pp. 395-410
Author(s):  
Tânia Mara Becher Ribas ◽  
◽  
Mikael Neumann ◽  
Egon Henrique Horst ◽  
Fernando Braga Cristo ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Experimental Design ◽  
Lactic Acid Bacteria ◽  
Propionic Acid ◽  
Dry Matter ◽  
Corn Silage ◽  
Bacterial Inoculants ◽  
Aerobic Stability

The objective was to evaluate the efficiency of two bacterial inoculants, 11CFT and 11C33, with different genera of lactic acid bacteria on the chemical and fermentation composition of the silage, and the temperature and pH behavior of the silage during the feed out period. The experimental design used was randomized blocks, with three treatments: corn silage without inoculant (control); corn silage with 11CFT inoculant (consisting of strains of Lactobacillus buchneri and L. casei); and corn silage with 11C33 inoculant (consisting of strains of L. buchneri, L. plantarum and Enterococcus faecium). The use of both inoculants increased the concentration of lactic acid in the silage (22.42 g kg-1 for control against 36.00 and 33.33 g kg-1 for 11CFT and 11C33, respectively) and reduced aerobic dry matter losses. The silage treated with 11C33 obtained a higher concentration of acetic acid (17.44 g kg-1) and propionic acid (2.08 g kg-1). The 11CFT inoculant provided a lower concentration of ethanol, however, without differing from the silage with 11C33 (0.70 and 1.61 g kg-1, respectively). Even without variations in temperature and pH at silage unloading, the use of the 11C33 inoculant generated a higher concentration of acetic and propionic acid, providing better aerobic stability days after unloading. Both inoculants also improved the in situ ruminal digestibility of corn silage compared to control silage. They provide an increase in the content of lactic and propionic acids, which assist to reduce dry matter losses and ethanol production. There were no variations in temperature and pH at the silo unloading, however, the use of the 11C33 inoculant generated a higher concentration of acetic and propionic acids providing better aerobic stability after exposure to air.

scholarly journals Corn grain processing improves chemical composition and fermentative profile of rehydrated silage

2018 ◽  
Vol 40 (1) ◽  
pp. 42564 ◽  
Author(s):  
Marlon Richard Hilário da Silva ◽  
Clóves Cabreira Jobim ◽  
Mikael Neumann ◽  
Milene Puntel Osmari
Keyword(s):  
Particle Size ◽  
Chemical Composition ◽  
Dry Matter ◽  
Nutritional Value ◽  
Geometric Mean ◽  
Grain Processing ◽  
Aerobic Stability ◽  
Mean Diameter ◽  
Ground Corn ◽  
Corn Grain

This study evaluated the effects of the rehydration and ensiling of corn grain with two geometric mean diameters (GMD) of 0.55 and 1.83 mm obtained by the diameter of the sieved used on the fermentation characteristics and chemical composition of the silage. The experimental design was completely randomized with two treatments and six replications, as follows: rehydrated fine-corn grain silage (RFS) and rehydrated coarse-corn grain silage (RCS). Ground corn was rehydrated with water to achieve moisture levels close to 350 g kg-1. There was no effect on the dry matter recovery, though the recovery rate was high, with values greater than 97% in both silages. The particle size influenced the fiber content, with lower values in the RFS. The acetic and lactic acid concentrations were higher in the RFS, but the pH of the silages did not change. Oxygen exposure changed the pH of silages to 4.25 and 4.38 for RFS and RCS, respectively. After opening the silos, the silages showed high aerobic stability after 90 hours, which resulted in lower deterioration and lesser loss of quality. The geometric mean diameter of corn grain affects the silage fermentative profile and nutritional value.  

scholarly journals Microbial succesion, fermentation end-products, aerobic stability of guinea grass ensiled with various doses of additive containing bacterial inoculant and fibrolytic enzymes.

1969 ◽  
Vol 85 (3-4) ◽  
pp. 151-164
Author(s):  
Abner A. Rodríguez ◽  
José L. Martínez ◽  
Raúl Macchiavelli ◽  
Ernesto O. Riquelme
Keyword(s):  
Lactic Acid ◽  
Chemical Composition ◽  
Dry Matter ◽  
Microbial Populations ◽  
Soluble Carbohydrate ◽  
Microbial Succession ◽  
Grass Silage ◽  
Aerobic Stability ◽  
End Products ◽  
Guinea Grass

An experiment was conducted to evaluate the effect of three application rates (0,1 and 2 times the recommended rate) of a commercial additive containing a lactic acid-producing bacterial inoculant as well as plant cell walldegrading enzymes, on the microbial succession, fermentation end-products, and aerobic stability of guinea grass (Panicum maximum var. Jacq.) silage. Vegetative material was harvested at 30% dry matter (DM) and chopped into 2.5-cm pieces. At ensiling, three treatments were imposed: no additive (control), additive applied at recommended rate, and at 2x the recommended rate. Three silos per treatment were opened after 0, 2, 4, 7, 14, 28, and 56 d of fermentation, and siiage was analyzed for pH, microbial succession, chemical composition, fermentation end-products and aerobic stability. For aerobic stability determination, three silos per treatment were opened at the end of the fermentation period, and silage (400 g) was exposed to air for three days in Styrofoam containers lined with plastic. After 0 , 1 , and 3 d of aerobic exposure, silage was analyzed for pH, microbial populations (total bacteria, yeast and molds), water soluble carbohydrate content, fermentation end-products and in vitro dry matter degradability (IVDMD). Temperature was monitored daily and dry matter recovery (DMR) was calculated after 1 and 3 d of aerobic exposure. The addition of the commercial additive, applied one or two times the recommended rate, increased (P < 0.05) the lactic acid producing bacterial population and decreased (P < 0.05) conforms during early stages of the fermentation process, but did not influence the yeast and mold populations or the chemical composition of the resulting silage. Use of the inoculant-enzyme mixture also resulted in siiage with higher lactic acid content 56 days post ensiling. The silage additive did not inffuence pH, temperature, microbial populations, soluble carbohydrate content, IVDMD or DMR of guinea grass silage after exposure to air. In summary, use of the commercial additive applied at the recommended rate partially improved the fermentation characteristics of guinea grass silage, but did not enhance its aerobic stability. Increasing the application rate to twice the recommended rate did not result in better fermentation.

scholarly journals The effects of three silage inoculants on aerobic stability in grass, clover-grass, lucerne and maize silages

2013 ◽  
Vol 22 (1) ◽  
pp. 137-144 ◽  
Author(s):  
Jonas Jatkauskas ◽  
Vilma Vrotniakiene ◽  
Christer Ohlsson ◽  
Bente Lund
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Chemical Composition ◽  
Lactic Acid Bacteria ◽  
Butyric Acid ◽  
Dry Matter ◽  
Red Clover ◽  
Laboratory Scale ◽  
Aerobic Stability ◽  
Silage Inoculants

The objective of the study was to investigate the effects of homofermentative and heterofermentative lactic acid bacteria (LAB) inoculants on fermentation and aerobic stability in a variety of crops and dry matter concentrations. The experiments were conducted with lucerne, ryegrass, ryegrass-timothy, red clover-ryegrass and whole crop maize using three additives in laboratory scale conditions. Each treatment and crop was replicated 5 five times when determining the chemical composition and aerobic stability in the silage. The data were statistically analyzed as a randomized complete block by using the GLM procedure of SAS. Additive application reduced pH and formation of butyric acid, alcohols and ammonia-N in all crops compared with the untreated silage (p < 0.05). The use of additives increased the content of lactic acid except heterofermentative LAB in maize with 276 g kg-1 DM and increased the content of acetic acid except homofermentative LAB in ryegrass-timothy and maize with 276 g kg-1 DM compared with the untreated silage (p < 0.05). It was observed that the aerobic stability of silages was improved significantly (p < 0.05) by using homofermentative and heterofermentative LAB inoculants.

scholarly journals Effects of processing, moisture, and storage length on the fermentation profile, particle size, and ruminal disappearance of reconstituted corn grain

2020 ◽  
Vol 98 (11) ◽  
Author(s):  
Ana L M Gomes ◽  
Antonio V I Bueno ◽  
Fernando A Jacovaci ◽  
Guilherme Donadel ◽  
Luiz F Ferraretto ◽  
...  
Keyword(s):  
Particle Size ◽  
Lactic Acid ◽  
Butyric Acid ◽  
Hammer Mill ◽  
Moisture Concentration ◽  
Fermentation Profile ◽  
And Storage ◽  
Ground Corn ◽  
Corn Grain

Abstract Our objective was to examine the effects of processing, moisture, and anaerobic storage length of reconstituted corn grain (RCG) on the fermentation profile, geometric mean particle size (GMPS), and ruminal dry matter disappearance (DMD). Dry corn kernels were ground (hammer mill, 5-mm screen) or rolled, then rehydrated to 30%, 35%, or 40% moisture, and stored for 0, 14, 30, 60, 90, 120, or 180 d in laboratory silos. Rolled corn had an increased GMPS compared with ground corn (2.24 and 1.13 mm, respectively, at ensiling). However, there was a trend for an interaction between processing and moisture concentration to affect particle size, with GMPS increasing with increased moisture concentration, especially in ground corn. Longer storage periods also slightly increased GMPS. Processing, moisture, and storage length interacted to affect the fermentation pattern (two- or three-way interactions). Overall, pH decreased, whereas lactic acid, acetic acid, ethanol, and NH3-N increased with storage length. RCG with 30% moisture had less lactic acid than corn with 35% and 40% moisture, indicating that fermentation might have been curtailed and also due to the clostridial fermentation that converts lactic acid to butyric acid. Ensiling reconstituted ground corn with 30% of moisture led to greater concentrations of ethanol and butyric acid, resulting in greater DM loss than grain rehydrated to 35% or 40% of moisture. Ammonia-N and in situ ruminal DMD were highest for reconstituted ground corn with 35% or 40% of moisture, mainly after 60 d of storage. Therefore, longer storage periods and greater moisture contents did not offset the negative effect of greater particle size on the in situ ruminal DMD of rolled RCG. Nonetheless, RCG should be ensiled with more than 30% moisture and stored for at least 2 mo to improve the ruminal DMD and reduce the formation of ethanol and butyric acid.

Comparison of supplemental cobalt form on fibre digestion and cobalamin concentrations in cattle

2017 ◽  
Vol 155 (5) ◽  
pp. 832-838 ◽  
Author(s):  
R. C. WATERMAN ◽  
W. L. KELLY ◽  
C. K. LARSON ◽  
M. K. PETERSEN
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
National Research Council ◽  
Residual Effect ◽  
Vitamin B ◽  
Serum Concentrations ◽  
Forage Digestibility ◽  
Randomized Experimental Design ◽  
To Receive

SUMMARYCobalt (Co) is essential for rumen microbial metabolism to synthesize methane, acetate and methionine. It also serves as a structural component of vitamin B12(cobalamin), which functions as a coenzyme in energy metabolism. A study was conducted to determine if Co form (carbonatev. glucoheptonate) supplemented above the National Research Council requirements would improve digestibility of a low-quality forage diet and change serum cobalamin concentrations. Nineteen ruminally cannulated cows (577 ± 13 kg) were fed individually in a completely randomized experimental design. Cows were fed a grass hay diet that contained (79·2 g/kg crude protein, 565 g/kg total digestible nutrients, 633·2 g/kg neutral detergent fibre (NDF), 874·2 g/kg dry matter) at a rate of 0·02% of body weight on a as fed basis for a 62-day study, which consisted of three periods; acclimation (AC), treatment (TR) and residual (RE). Measurements taken in the AC period were used as covariates for analysis in the TR and RE periods. Cows were stratified by age (5 ± 0·4 years) and lactational history, and assigned to receive 12·5 mg supplemental Co in one of two forms: (1) 27·2 mg of Co carbonate (CC,n= 11 cows) or (2) 50 mg of Co glucoheptonate (CGH,n= 8 cows). Supplement was administered daily via a gelatin capsule placed directly into the rumen 2 h after feeding. During the last 96 h of each period, forage digestibility was measured using anin situnylon bag technique. Blood samples were collected 4 and 6 h following feeding, and 24 h before the end of each period. A treatment × period interaction was detected forin situorganic matter (OM) disappearance at 96 h; (TR period: 684 and 708 ± 81 g/kg; RE period: 676 and 668 ± 75 g/kg, for CC and CGH, respectively). Once inclusion of Co in the CGH group was removed, OM disappearance was reduced by 4·01% compared with 0·82% in the CC cows. The NDF disappearance (OM basis) was less for the TR compared with the RE at 48 h (629 and 652 ± 39 g/kg, respectively). However, by 96 h NDF disappearance was greater for TR than the RE (704 and 689 ± 44 g/kg; respectively). No differences were detected for cobalamin serum concentrations or rate of fibre fermentation. The outcomes of the current research signify that there may be a slight residual effect of Co supplementation on fermentation; there was also an indication that Co source may enhance the overall extent of fermentation.

scholarly journals Fermentation profile and chemical composition of cassava root silage

2021 ◽  
Vol 51 (3) ◽  
pp. 191-198
Author(s):  
Juliana Schuch PITIRINI ◽  
Rosana Ingrid Ribeiro dos SANTOS ◽  
Francy Manoely da Silva LIMA ◽  
Ilano Silva Braga do NASCIMENTO ◽  
Jehmison de Oliveira BARRADAS ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Soil Acidity ◽  
Neutral Detergent Fiber ◽  
Fresh Matter ◽  
Cassava Root ◽  
Aerobic Stability ◽  
Fermentation Profile ◽  
Silage Production ◽  
High Level

ABSTRACT The use of cassava root silage for animal feeding is a suitable option for farmers who grow cassava as an alternative product and for cattle ranchers who have to deal with high prices of corn. Our objective was to determine the effects of cassava genotypes and the correction of soil acidity on the microbial population, fermentation characteristics, chemical composition, aerobic stability and losses of cassava root silage. We used a 2 × 3 factorial design in completely randomized blocks, with four replications. We evaluated two cassava genotypes (Caeté and Manteiguinha) and three methods of soil acidity correction (lime, gypsum, and lime+gypsum). The roots were harvested 11 months after planting, ensiled in PVC silos, and stored for 45 days. No interaction was observed between genotypes and soil acidity correction for any of the evaluated parameters. The silage of Caeté genotype showed the highest concentration of dry matter (421 g kg-1 fresh matter) and non-fibrous carbohydrates (893 g kg-1 dry matter), and the lowest concentrations of neutral detergent fiber (37.1 g kg-1 dry matter) . No significant differences were observed among treatments for lactic acid bacteria, yeast and mold counts in silages. Both genotypes resulted in silages with an adequate fermentation profile and considerably high aerobic stability, but with high effluent loss. The Caeté genotype showed to be potentially better for silage production due to its higher dry matter recovery. Due to the high level of effluent loss, it is recommended to test the effect of a moisture-absorbing additive during the ensiling process of these cassava roots.

scholarly journals Nutritional value, fermentation losses and aerobic stability of elephant grass (Pennisetum purpureum Schum.) silage treated with exogenous fibrolytic enzymes

2020 ◽  
Vol 42 ◽  
pp. e48272
Author(s):  
Maikon Figueredo Lemos ◽  
Alexandro Pereira Andrade ◽  
Pedro Henrique Ferreira da Silva ◽  
Camila Oliveira Santos ◽  
Caio Felipe Barros Souza ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Nutritional Value ◽  
Pennisetum Purpureum ◽  
Elephant Grass ◽  
Fibrolytic Enzymes ◽  
Grass Silage ◽  
Aerobic Stability ◽  
Pennisetum Purpureum Schum

The aim of this study was to evaluate nutritional value, fermentation losses, and aerobic stability of elephant grass silage (Pennisetum purpureum Schum.) treated with exogenous fibrolytic enzymes. The experiment was conducted in a completely randomized design with four replicates (experimental silos) and five levels of fibrolytic enzymes (0, 1.5, 3.0, 4.5 and 6.0%). For this, the elephant grass was ensiled at 70 days of age in plastic buckets with 20L capacity. Silos were opened 60 days after sealing. Analyses were made for chemical composition, in vitro dry matter digestibility (IVDMD), effluent losses (EL), gas losses (GL) and dry matter recovery (DMR), as well as the aerobic stability of the silage. Data were analyzed with PROC REG of SAS® University, at 5% probability. There was an increase in IVDMD content (p < 0.0001) and reduction in NDF and ADF contents (p < 0.0001) according to enzyme levels. These results were related to the increase in the degradation of fiber fractions. There were higher EL (p = 0.0062) as a function of enzyme levels and aerobic deterioration after silo opening, at all levels tested. Thus, it can be concluded that the exogenous fibrolytic enzymes change the chemical composition of elephant grass silage, and increase its digestibility and nutritional value. Moreover, when used alone as an additive, fibrolytic enzymes are not able to recover all dry matter of this silage (with effluent and gas losses), and are not able to maintain aerobic stability in the first hours after opening the silos.

Fermentative characteristics and chemical composition of cochineal nopal cactus silage containing chemical and microbial additives

2020 ◽  
pp. 1-9
Author(s):  
W. C. C. S. Sá ◽  
E. M. Santos ◽  
J. S. de Oliveira ◽  
G. G. L. de Araujo ◽  
A. F. Perazzo ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Chemical Composition ◽  
Ph Value ◽  
Stability Test ◽  
Aerobic Stability ◽  
Constant Ph ◽  
The Stability ◽  
Exclusive Or ◽  
Storage Times ◽  
Silage Additives

Abstract The objective of this study was to evaluate the fermentative characteristics and chemical composition of cochineal nopal cactus silage additives with urea or Lactobacillus buchneri (LB), as well as the association of both additives in four storage times (7, 15, 60 and 120 days) and during aerobic stability, with evaluations at 0, 48 and 96 h. Four silages were used: no additive, addition of 2% urea, addition of LB and addition of 2% urea and LB. The study was divided into two experiments: the first experiment evaluated the silages at different storage times, and the second experiment evaluated the silages during the aerobic stability test. In both experiments, the experimental design was completely randomized in a factorial scheme (4 × 4 and 4 × 3) with three replicates per treatment. After the ensiling process, lactic acid bacteria predominated in all treatments. The concentration of lactic acid increased significantly from 60 days of ensiling. The concentration of acetic acid varied significantly between the storage times only for the silages treated with urea and LB alone. The silage treated with urea maintained a constant pH value up to 120 days of storage. During the 96 h aerobic stability test, no breaking in the stability of silages was observed. The exclusive or associated use of urea and LB promotes improvement in the fermentative characteristics of cochineal nopal cactus silage, without major alterations in the chemical composition or interfering with the aerobic stability of the silages.

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