Effect of acidification of sheep's cheese whey with organic acids on the retention of protein and on the quality of Myzithra cheese

1988 ◽  
Vol 55 (2) ◽  
pp. 247-254 ◽  
Author(s):  
Christophoros Pappas ◽  
Leandros Voutsinas
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Organic Acids ◽  
Whey Protein ◽  
Cheese Whey ◽  
Protein Retention

SummaryWhen 10% citric, lactic or acetic acid was added to sheep's milk cheese whey during its heating for Myzithra cheese preparation, protein retention in the cheese was increased. Acidification of whey to pH 5·2 with lactic acid before heating followed by readjustment to pH 5·8 with NaOH was considered to be the best treatment for practical use, increasing whey protein retention in the cheese and yield without loss of flavour, aroma or texture.

scholarly journals Potential Sustainable Properties of Microencapsulated Endophytic Lactic Acid Bacteria (KCC-42) in In-Vitro Simulated Gastrointestinal Juices and Their Fermentation Quality of Radish Kimchi

2018 ◽  
Vol 2018 ◽  
pp. 1-10
Author(s):  
Chae Eun Song ◽  
Han Hyo Shim ◽  
Palaniselvam Kuppusamy ◽  
Young-IL Jeong ◽  
Kyung Dong Lee
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Organic Acids ◽  
Succinic Acid ◽  
Fermentation Medium ◽  
Gastrointestinal Fluid ◽  
Fermentation Quality

The objective of this study was to investigate alginate microencapsulated lactic acid bacteria (LAB) fermentation quality of radish kimchi sample and its potential survivability in different acidic and alkaline environments. Initially, we isolated 45 LAB strains. One of them showed fast growth pattern with potential probiotic and antifungal activities against Aspergillus flavus with a zone of inhibition calculated with 10, 8, 4mm for the 4th, 5th, and 6th day, respectively. Therefore, this strain (KCC-42) was chosen for microencapsulation with alginate biopolymer. It showed potential survivability in in-vitro simulated gastrointestinal fluid and radish kimchi fermentation medium. The survival rate of this free and encapsulated LAB KCC-42 was 6.85 × 105 and 7.48× 105 CFU/ml, respectively; the viability count was significantly higher than nonencapsulated LAB in simulated gastrointestinal juices (acid, bile, and pancreatin) and under radish kimchi fermentation environment. Kimchi sample added with this encapsulated LAB showed increased production of organic acids compared to nonencapsulated LAB sample. Also, the organic acids such as lactic acid, acetic acid, propionic acid, and succinic acid production in fermented kimchi were measured 59mM, 26mM, 14mM, and 0.6mM of g/DW, respectively. The production of metabolites such as lactic acid, acetic acid, and succinic acid and the bacteria population was high in microencapsulated LAB samples compared with free bacteria added kimchi sample. Results of this study indicate that microencapsulated LAB KCC-42 might be a useful strategy to develop products for food and healthcare industries.

scholarly journals INFLUENCE OF STATE HAY WITH BIOLOGICAL CONSERVATIVES ON MILK AND CHEESE QUALITY

2019 ◽  
pp. 84-89 ◽  
Author(s):  
Анна Карамаева ◽  
Anna Karamayeva ◽  
Сергей Карамаев ◽  
Sergey Karamaev ◽  
Наталья Соболева ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Organic Acids ◽  
Crude Protein ◽  
Nutritional Value ◽  
Fat Content ◽  
Animal Feeding ◽  
Digestible Protein ◽  
Biological Preservative

The purpose of the study is to improve the property of eastern galega haylage by using verdant grass preservative applicator and biologies. Iit was established that the eastern galega verdant grass, because of the low sugar con-tent, is not good for state hay purchasing due to the of the obtained results. The biological preservative applicators use for state hay purchasing significantly improves the quality of feed. The preservatives use for verdant grass al-lowed to increase the active acidity of the finished feed in the range between 6.3 to 11.2%, the content of lactic acid – 0.51-0.84 per cent and to reduce the content of acetic acid by 0.19 and 0.27%, respectively. The proportion of lactic acid in the total volume of organic acids was increased by 13.03-16.88%.The nutritional value of haylage with bio-logical preservative applicants increased due to augment of the dry content by 1.7-3.2%, EKE – by 4.4-11.1%, crude protein – by 4.3-10.1%, digestible protein – by 7.4-14.7%.The coefficient of digestibility in the diet of dry con-tent increased by 1.14-3.19%, crude protein – by 2.14-3.62%, crude fat – by 1.64-4.09%, crude fiber – by 1.74-3.87%, BEV – by 2.05-4.13%, which is very important for animal feeding. The use of haylage with a preservative in the diet of cows from experimental groups increased the fat content in milk by 0.03-0.08%, protein – by 0.04-0.10%, and casein contained – by 0.07-0.18%. Rennet additive apply helps milk to coagulate faster by 2.3-4.5 min (6.6-12.8%), and decrease losses of dry content with serum by 1.5-3.0%. The dry content mass in the experimental samples of cheese increased by 2.8-4.7%, fat – by 1.6-2.7%, protein – by 0.7-1.6%, the degree of cheese maturity improved. The «Milostan and Loktantra + zeolite» preservatives provide better properties for products produced.

scholarly journals THE EFFECT OF HOMO - AND HETERO - FERMENTATIVE LACTIC ACID BACTERIA MIX ON THE ENSILED LUCERNE FERMENTATION CHARACTERISTICS AND AEROBIC STABILITY IN BIG BALES

2018 ◽  
Author(s):  
Robertas STOŠKUS ◽  
Jonas JATKAUSKAS ◽  
Vilma VROTNIAKIENĖ ◽  
Vida JUOZAITIENĖ
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Organic Acids ◽  
Lactococcus Lactis ◽  
Butyric Acid ◽  
Maximum Temperature ◽  
N Concentration ◽  
Aerobic Stability

The purpose of this study was to determine the effect of homo- and hetero-fermentative lactic acid bacteria mix on the ensiled lucerne fermentation characteristics and aerobic stability in big bales. The lucerne was ensiled without additives (C) and treated with a mix of bacterial inoculant that contains Lactococcus lactis and Lactobacillus buchneri (50:50) (I). Silage was treated with bacterial inoculant, which significantly increased the total organic acids concentration by 69 %, lactic acid by 92% and acetic acid by 76 %. If the results were compared with the C silage, the inoculation significantly decreased the concentrations of butyric acid by 73 %, ethanol by 53 % and ammonia - N concentration by 33%. Inoculated silage had significantly lowered the yeast count by 59 % and moulds count by 34 %. Compared to the inoculated silage and during the aerobic exposure, the untreated silage maximum temperature was significantly higher (13.9 0C vs 4.6 0C) (P < 0.05). Therefore, the bacterial inoculant improved the quality of fermentation and aerobic stability in lucerne silages.

Mitigating the Antimicrobial Activities of Selected Organic Acids and Commercial Sanitizers with Various Neutralizing Agents

2011 ◽  
Vol 74 (5) ◽  
pp. 820-825 ◽  
Author(s):  
YOEN JU PARK ◽  
JINRU CHEN
Keyword(s):  
Escherichia Coli ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Organic Acids ◽  
Shiga Toxin ◽  
Room Temperature ◽  
Sodium Thiosulfate ◽  
Antimicrobial Activities ◽  
Phosphate Buffered Saline ◽  
Lactic Acids

This study was conducted to evaluate the abilities of five neutralizing agents, Dey-Engley (DE) neutralizing broth (single or double strength), morpholinepropanesulfonic acid (MOPS) buffer, phosphate-buffered saline (PBS), and sodium thiosulfate buffer, in mitigating the activities of acetic or lactic acid (2%) and an alkaline or acidic sanitizer (a manufacturer-recommended concentration) againt the cells of Shiga toxin–producing Escherichia coli (STEC; n = 9). To evaluate the possible toxicity of the neutralizing agents to the STEC cells, each STEC strain was exposed to each of the neutralizing agents at room temperature for 10 min. Neutralizing efficacy was evaluated by placing each STEC strain in a mixture of sanitizer and neutralizer under the same conditions. The neutralizing agents had no detectable toxic effect on the STEC strains. PBS was least effective for neutralizing the activity of selected organic acids and sanitizers. Single-strength DE and sodium thiosulfate neutralized the activity of both acetic and lactic acids. MOPS buffer neutralized the activity of acetic acid and lactic acid against six and five STEC strains, respectively. All neutralizing agents, except double-strength DE broth, had a limited neutralizing effect on the activity of the commercial sanitizers used in the study. The double-strength DE broth effectively neutralized the activity of the two commercial sanitizers with no detectable toxic effects on STEC cells.

scholarly journals Effect of Co-Inoculation with Saccharomyces cerevisiae and Lactic Acid Bacteria on the Content of Propan-2-ol, Acetaldehyde and Weak Acids in Fermented Distillery Mashes

2019 ◽  
Vol 20 (7) ◽  
pp. 1659
Author(s):  
Katarzyna Pielech-Przybylska ◽  
Maria Balcerek ◽  
Grzegorz Ciepielowski ◽  
Barbara Pacholczyk-Sienicka ◽  
Łukasz Albrecht ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Acid Content ◽  
Isopropyl Alcohol ◽  
Quantitative Composition ◽  
Acetic Acid Content ◽  
Initial Ph

The qualitative and quantitative composition of volatile compounds in fermented distillery mash determines the quality of the obtained distillate of agricultural origin (i.e., raw spirit) and the effectiveness of further purification steps. Propan-2-ol (syn. isopropyl alcohol), due to its low boiling point, is difficult to remove by rectification. Therefore, its synthesis needs to be limited during fermentation by Saccharomyces cerevisiae yeast, while at the same time controlling the levels of acetaldehyde and acetic acid, which are likewise known to determine the quality of raw spirit. Lactic acid bacteria (LAB) are a common but undesirable contaminant in distillery mashes. They are responsible for the production of undesirable compounds, which can affect synthesis of propan-2-ol. Some bacteria strains are able to synthesize isopropyl alcohol. This study therefore set out to investigate whether LAB with S. cerevisiae yeast are responsible for conversion of acetone to propan-2-ol, as well as the effects of the amount of LAB inoculum and fermentation parameters (pH and temperature) on the content of isopropyl alcohol, acetaldehyde, lactic acid and acetic acid in fermented mashes. The results of NMR and comprehensive two-dimensional gas chromatography coupled with time of flight mass spectrometry (GC × GC-TOF MS) analysis confirmed the ability of the yeast and LAB strains to metabolize acetone via its reduction to isopropyl alcohol. Efficient fermentation of distillery mashes was observed in all tested mashes with an initial LAB count of 3.34–6.34 log cfu/mL, which had no significant effect on the ethanol content. However, changes were observed in the contents of by-products. Lowering the initial pH of the mashes to 4.5, without and with LAB (3.34–4.34 log cfu/mL), resulted in a decrease in propan-2-ol and a concomitant increase in acetaldehyde content, while a higher pH (5.0 and 5.5) increased the content of propan-2-ol and decreased acetaldehyde content. Higher temperature (35 °C) promoted propan-2-ol synthesis and also resulted in increased acetic acid content in the fermented mashes compared to the controls. Moreover, the acetic acid content rose with increases in the initial pH and the initial LAB count.

Effects of Fermented Green Juice and Cellulase on the Silage Quality of Sugarcane Top

2012 ◽  
Vol 554-556 ◽  
pp. 1053-1056 ◽  
Author(s):  
Yi Fen Zhuang ◽  
Xin Zhu Chen ◽  
Zhao Xia Dong ◽  
Jian Guo Zhang ◽  
Wen Chang Zhang
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Moisture Content ◽  
Water Soluble ◽  
Soluble Carbohydrate ◽  
Moisture Contents ◽  
Ph Values ◽  
Silage Quality ◽  
Water Soluble Carbohydrate

Two moisture levels of sugarcane top (moisture contents 69.03% - MC1 and 56.38% - MC2) were treated with fermented green juice (FGJ) at 2 ml/kg and/or with cellulase (CEL) at 5000 U/kg raw matter, and ensiled in the laboratory at ambient temperature. All additive treatments significantly (P<0.01) increased lactic acid and water soluble carbohydrate (WSC) contents, and significantly (P<0.01) decreased pH and contents of NH3-N and acetic acid compared with the control. The treatment of FGJ and CEL mixture significantly (P<0.01) decreased pH and content of acetic acid (AA) compared with treatments of FGJ and CEL alone. All the MC2 silages had more extensive fermentation than MC1 silage, shown by higher contents of lactic acid, lower pH values and NH3-N contents. In conclusion, both FGJ and CEL addition significantly improved the silage quality of sugarcane top, and their mixture had better result than FGJ and CEL alone. Reducing the moisture content of sugarcane top could improve its silage quality.

scholarly journals Biochemistry of waterlogged soils. Part III: Decomposition of carbohydrates with special reference to formation of organic acids

1929 ◽  
Vol 19 (4) ◽  
pp. 627-648 ◽  
Author(s):  
V. Subrahmanyan
Keyword(s):  
Carbon Dioxide ◽  
Organic Matter ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Organic Acids ◽  
Pyruvic Acid ◽  
Soluble Nitrogen ◽  
Direct Oxidation ◽  
Micro Organisms ◽  
Organic Matter Addition

(1) In absence of decomposing organic matter addition of nitrate led to no loss of nitrogen.(2) On addition of small quantities of fermentable matter such as glucose there was (a) rapid depletion of nitrates and oxygen, but no denitrification, and (b) increase in acidity, carbon dioxide and bacteria. The greater part of the soluble nitrogen was assimilated by microorganisms or otherwise converted and the greater part of the added carbohydrate was transformed into lactic, acetic and butyric acids.(3) The organic acids were formed from a variety of carbohydrates. Lactic acid was the first to be observed and appeared to be formed mainly by direct splitting of the sugar. It decomposed readily, forming acetic and butyric acids. Some acetic acid was formed by direct oxidation of lactic acid, with pyruvic acid as the intermediate product. All the acids were, on standing, converted into other forms by micro-organisms.

scholarly journals QUALITY OF ALFALFA SENAGE WITH THE«GREENGRASSE 3×3» BIOPRESERVATIVE

2021 ◽  
Vol 6 (1) ◽  
pp. 44-51
Author(s):  
Nikolai Mironov ◽  
Anna Karamayeva ◽  
Sergei Karamayev ◽  
Larisa Bakayeva
Keyword(s):  
Lactic Acid ◽  
Organic Acids ◽  
Volatile Fatty Acids ◽  
Dry Weight ◽  
Acid Fermentation ◽  
Protein Nitrogen ◽  
Available Energy ◽  
Production Complex ◽  
Positive Effect

The aim of the research is to improve the quality and nutritional value of the alfalfa haylage applying the «Green-grass 3×3» biopreservative with herbage. Current biological preparations contain live cultures of specially selected lactic, propionic acids, and sporogenous bacillus, a complex of amino acids, enzymes, vitamins and minor nutrient elements. Preparation use with herbage during haylage leads to intensification the lactic acid fermentation, im-provement of ratio of organic acids, increase the content of nutrients and ensuring their reliable safety during stor-age. The research was carried out at the milk production complex of «Radna» LLC in the Samara region. The re-search was provided involving Holstein and Ayrshire breeds. The haylage was fed to cows in the amount of 24 kg as part of a balanced feed mixture in accordance with the casein diet. As a result of applying a bio-preservative with herbage, the content of organic acids in the ready made feeds increased by 0.54%, including lactic acid – by 0.80%. The dry weight increased by 4.6%, available energy – by 37.4%, energy feed units – by 9.1%, digestible protein – by 13.8%, crude fiber – by 0.9%. Feeding the cows haylage with a bioconservative increased the meta-bolic processes in the tripe of animals. Ciliata weight in the ruminal fluid of Holstein cows increased by 29.0%, Ayr-shire – by 25.8%, number of bacteria, respectively, by 26.5 and 27.5%. As a result, the content of total nitrogen in-creased by 15.0-15.1%, protein nitrogen – by 40.4-42.9%, and ammonia, on the contrary, decreased by 40.0-40.6%. The content of volatile fatty acids decreased by 12.6-16.2%, while the proportion of acetic acid increased by 7.73-8.71%. All this had a positive effect on the digestibility of nutrients by the animals of the experimental groups. The digestibility of the dry weight of the feed increased, respectively, by 4.14-4.57%, and its components – by 4.23-6.97%.

scholarly journals Impact of preoperative fecal short chain fatty acids on postoperative infectious complications in esophageal cancer patients

2019 ◽  
Author(s):  
Masaaki Motoori ◽  
Koji Tanaka ◽  
Keijiro Sugimura ◽  
Hiroshi Miyata ◽  
Takuro Saito ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Organic Acids ◽  
Propionic Acid ◽  
Butyric Acid ◽  
Bacterial Translocation ◽  
Infectious Complications ◽  
The Body ◽  
Intestinal Environment

Abstract Background: The intestinal epithelial barrier allows absorption of dietary nutrients and prevents passage of pathogens and toxins into the body. Severe insults have a negative impact on the intestinal environment, which may decrease intestinal barrier function and cause bacterial translocation. Bacterial translocation, which can cause infectious complications, is the passage of microbes from the gastrointestinal tract across the mucosal barrier to extraintestinal sites. The aim of this study was to investigate the correlation between concentrations of preoperative fecal organic acids and the occurrence of postoperative infectious complications in patients with esophageal cancer. Methods: Fifty-five patients with esophageal cancer who underwent esophagectomy were enrolled in this study. All patients were administered perioperative synbiotics. Perioperative clinical characteristics and concentrations of preoperative fecal organic acids were compared between patients with or without postoperative infectious complications. Results: Postoperative infectious complications occurred in 10 patients. In patients with complications, the concentrations of acetic acid and propionic acid were significantly lower than in patients without complications (p=0.044 and 0.032, respectively). The concentration of butyric acid was nonsignificantly lower, while the concentration of lactic acid was nonsignificantly higher in patients with complications. The calculated gap between the concentrations of fecal acetic acid plus propionic acid plus butyric acid minus lactic acid was significantly lower in patients with complications. Multivariate analysis revealed that a low gap between acetic acid plus propionic acid plus butyric acid minus lactic acid was an independent risk factor for postoperative infectious complications (p=0.027). Conclusions : Preoperative fecal concentrations of organic acids had a clinically important impact on the occurrence of postoperative infectious complications in patients with esophageal cancer. To reduce postoperative infectious complications, it may be useful to modulate the intestinal environment and maintain concentrations of fecal organic acids before surgery.

scholarly journals Impact of preoperative fecal short chain fatty acids on postoperative infectious complications in esophageal cancer patients

2020 ◽  
Author(s):  
Masaaki Motoori ◽  
Koji Tanaka ◽  
Keijiro Sugimura ◽  
Hiroshi Miyata ◽  
Takuro Saito ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Organic Acids ◽  
Propionic Acid ◽  
Butyric Acid ◽  
Bacterial Translocation ◽  
Infectious Complications ◽  
The Body ◽  
Intestinal Environment

Abstract Background: The intestinal epithelial barrier allows absorption of dietary nutrients and prevents passage of pathogens and toxins into the body. Severe insults have a negative impact on the intestinal environment, which may decrease intestinal barrier function and cause bacterial translocation. Bacterial translocation, which can cause infectious complications, is defined as the passage of microbes from the gastrointestinal tract across the mucosal barrier to extraintestinal sites. The aim of this study was to investigate the correlation between concentrations of preoperative fecal organic acids and the occurrence of postoperative infectious complications in patients with esophageal cancer. Methods: Fifty-five patients with esophageal cancer who underwent esophagectomy were enrolled in this study. Perioperative synbiotics were administered to all patients. Perioperative clinical characteristics and concentrations of preoperative fecal organic acids were compared between patients with and without postoperative infectious complications. Results: Postoperative infectious complications occurred in 10 patients. In patients with complications, the concentrations of acetic acid and propionic acid were significantly lower than in patients without complications (p=0.044 and 0.032, respectively). The concentration of butyric acid was nonsignificantly lower in patients with complications, while the concentration of lactic acid was nonsignificantly higher. The calculated gap between the concentrations of fecal acetic acid plus propionic acid plus butyric acid minus lactic acid was significantly lower in patients with complications. Multivariate analysis revealed that a low gap between acetic acid plus propionic acid plus butyric acid minus lactic acid was an independent risk factor for postoperative infectious complications (p=0.027). Conclusions : Preoperative fecal concentrations of organic acids had a clinically important impact on the occurrence of postoperative infectious complications in patients with esophageal cancer. To reduce postoperative infectious complications, it may be useful to modulate the intestinal environment and maintain concentrations of fecal organic acids before surgery.

Sign in / Sign up

Export Citation Format

Share Document