Species identification of staphylococci and micrococci isolated from ewes' milk cheeses

1988 ◽  
Vol 55 (2) ◽  
pp. 269-276 ◽  
Author(s):  
María Camino García ◽  
Andrés Otero ◽  
María Luisa García ◽  
María Rosario García ◽  
Benito Moreno
Keyword(s):  
Staphylococcus Aureus ◽  
Species Identification ◽  
Species Level ◽  
The Other ◽  
Sensitive Species ◽  
Coagulase Negative Staphylococci ◽  
Resistant Strains

SummaryForty-five strains of staphylococci and 6 strains of micrococci isolated from pasteurized ewes' milk cheeses (Manchego and Burgos varieties) were identified to species level. Three strains of staphylococci were identified asStaphylococcus aureus. Amongst the 42 coagulase-negative staphylococci, 8 were novobiocin-sensitive and 34 novobiocin-resistant. The novobiocin-sensitive species found wereStaph. auricularis(5 strains),Staph. caseolyticus(2 strains) andStaph. epidermidis(1 strain). Novobiocin-resistant strains were identified asStaph. gallinarum(5 strains),Staph. xylosus(4),Staph. saprophyticus(9),Staph. cohnii(6),Staph. sciuri(1) andStaph. lentus(3). The other 6 isolates could not be identified. Only 3 strains of micrococci were identified (Micrococcus kristinae, M. variansandM. sedentarius). The possible origin of the isolates is discussed.

scholarly journals Interpretive criteria to differentiate low- and high-level mupirocin resistance in Staphylococcus aureus

2007 ◽  
Vol 56 (7) ◽  
pp. 937-939 ◽  
Author(s):  
Naira Elane Moreira de Oliveira ◽  
Ana Paula Couto Marques Cardozo ◽  
Elizabeth de Andrade Marques ◽  
Kátia Regina Netto dos Santos ◽  
Marcia Giambiagi deMarval
Keyword(s):  
Staphylococcus Aureus ◽  
High Resistance ◽  
The Other ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Zone Diameter ◽  
Resistant Strains ◽  
Mupirocin Resistance ◽  
High Level

Meticillin-resistant Staphylococcus aureus isolates were classified into three mupirocin susceptibility groups by the disc diffusion method using 5 and 200 μg mupirocin discs. The zone diameter observed for a 5 μg disc distinguished MupS from the resistant strains (either MupRL or MupRH). On the other hand, a 200 μg disc distinguished the high-resistance MupRH strains from the other two (MupS or MupRL). Thus, the concomitant use of 5 and 200 μg mupirocin discs allowed the clear distinction among the three mupirocin susceptibility groups, MupS, MupRL or MupRH.

Methicillin-ResistantS. aureusandS. epidermidisStrains: Modern Hospital Pathogens

1986 ◽  
Vol 7 (S2) ◽  
pp. 118-119 ◽  
Author(s):  
Richard P. Wenzel
Keyword(s):  
Staphylococcus Aureus ◽  
Infection Control ◽  
Weight Of Evidence ◽  
Effective Control ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Nosocomial Pathogens ◽  
International Recognition ◽  
Resistant Strains ◽  
Modern Hospital

The international recognition of the emergence of methicillin-resistantStaphylococcus aureusas major nosocomial pathogens dates to the late 1970s. The weight of evidence from many institutions suggests that transmission involves spread from patient to patient of the same or very similar strain and that effective control, if not elimination, involves isolation of infected and colonized patients.More recently, a number of reports have suggested the emergence of methicillin-resistantS. epidermidisand other coagulase-negative staphylococci as significant nosocomial pathogens. Although the organisms undoubtedly share many similarities with methicillin-resistant strains ofS. aureus, it is certain that biologic differences exist which are important for infection control.

Study of the resistance pattern and alteration in the conjunctival flora of patients exposed to repeated antibiotics

2020 ◽  
Vol 1 (4) ◽  
pp. 207-212
Author(s):  
Rania Abdelmonem Khattab ◽  
◽  
Iman Fahmy ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Resistance Pattern ◽  
Base Line ◽  
Coagulase Negative Staphylococci ◽  
Noticeable Increase ◽  
Diffusion Technique ◽  
Repeated Use ◽  
Conjunctival Flora ◽  
Resistant Strains

AIM: To investigate and analyze the changes in the conjunctival flora of patients exposed to repeated antibiotic usage. In addition, determining the effects of this repeated exposure on the resistance pattern of the conjunctival flora. METHODS: This study included 40 patients, admitted to the Retina Unit of the Research Institute of Ophthalmology. All chosen patients had a minimum of 4 consecutive, monthly intravitreal injections (IVI), some were extended to 6mo or 1y as required. The patients were randomly divided into 3 groups and each group received one kind of antibiotic which was either ofloxacin, moxifloxacin or ceftazidime. All bacterial isolates were tested for antibiotic susceptibility using Kirby-Bauer disc diffusion technique. RESULTS: In this study the conjunctival normal flora at base line culture varied from a predominance of Staphylococcus epidermidis (51.2%), followed by Staphylococcus aureus 14% to Micrococcus species 12.8% and other coagulase negative staphylococci (CONS) 13%. Our results showed an increase in the percentage of Staphylococcus epidermidis among ceftazidime treated eyes during the four visits in comparison to baseline cultures of patients randomized to ceftazidime. In contrast, there was no noticeable increase in Staphylococcus aureus percentage from baseline. In fluoroquinolone treated eyes, we also observed an increase in percentage of Staphylococcus epidermidis from base line. In contrast to ceftazidime treated eyes, the Staphylococcus aureus percentage in fluoroquinolone treated eyes showed an increase from baseline. However, the pattern of the ocular flora composition changed with the exposure to the old and newer generation of fluoroquinolones. We noticed an increase of Staphylococcus epidermidis in moxifloxacin treated eyes than that in ofloxacin treated eyes from baseline. There was no observed difference in the pattern of Staphylococcus aureus regarding exposure to older and newer generations of fluoroquinolones. CONCLUSION: Repeated use of ophthalmic antibiotics not only alters the composition of the normal ocular flora, but also selects for resistant strains.

scholarly journals Genetic Basis of Resistance to Fusidic Acid in Staphylococci

2007 ◽  
Vol 51 (5) ◽  
pp. 1737-1740 ◽  
Author(s):  
A. J. O'Neill ◽  
F. McLaws ◽  
G. Kahlmeter ◽  
A. S. Henriksen ◽  
I. Chopra
Keyword(s):  
Staphylococcus Aureus ◽  
Fusidic Acid ◽  
Drug Target ◽  
Genetic Basis ◽  
Elongation Factor ◽  
The Other ◽  
Clinical Isolates ◽  
Staphylococcal Species ◽  
Clinical Strains ◽  
Resistant Strains

ABSTRACT Resistance to fusidic acid in Staphylococcus aureus often results from acquisition of the fusB determinant or from mutations in the gene (fusA) that encodes the drug target (elongation factor G). We now report further studies on the genetic basis of resistance to this antibiotic in the staphylococci. Two staphylococcal genes that encode proteins exhibiting ca. 45% identity with FusB conferred resistance to fusidic acid in S. aureus. One of these genes (designated fusC) was subsequently detected in all fusidic acid-resistant clinical strains of S. aureus tested that did not carry fusB or mutations in fusA, and in strains of S. intermedius. The other gene (designated fusD) is carried by S. saprophyticus, explaining the inherent resistance of this species to fusidic acid. Fusidic acid-resistant strains of S. lugdunensis harbored fusB. Thus, resistance to fusidic acid in clinical isolates of S. aureus and other staphylococcal species frequently results from expression of FusB-type proteins.

scholarly journals Clinical Importance of Identifying Coagulase-Negative Staphylococci Isolated from Blood Cultures: Evaluation of MicroScan Rapid and Dried Overnight Gram-Positive Panels versus a Conventional Reference Method

1998 ◽  
Vol 36 (7) ◽  
pp. 2089-2092 ◽  
Author(s):  
Melvin P. Weinstein ◽  
Stanley Mirrett ◽  
Linda Van Pelt ◽  
Mary McKinnon ◽  
Barbara L. Zimmer ◽  
...  
Keyword(s):  
Species Identification ◽  
Clinical Importance ◽  
Reference Method ◽  
Common Species ◽  
The Other ◽  
Coagulase Negative Staphylococci ◽  
Gram Positive ◽  
Positive Identification ◽  
Clinically Significant ◽  
Staphylococcus Hominis

We evaluated the clinical usefulness of species identification of blood isolates of coagulase-negative staphylococci as a predictor of the clinical significance of the isolates. In addition, we compared results of species identification obtained with MicroScan Rapid Gram-Positive Identification panels and Dried Overnight (Conventional) Gram-Positive Identification panels with those obtained by a tube reference method. Two hundred eighty-five blood isolates were tested, including 92 judged to represent true bacteremia and 193 judged to represent contamination. The most common species detected wereStaphylococcus epidermidis, Staphylococcus hominis, and Staphylococcus haemolyticus. These three species accounted for nearly 98% of the clinically significant isolates and 89% of the contaminants. The isolation of other species almost always represented contamination. However, identification of the three most common species did not help distinguish pathogens from contaminants. Both the Rapid and the Dried Overnight Gram-Positive panels identified S. epidermidis strains accurately, but the panels performed less well for the other species. Analysis revealed that S. hominis was frequently misidentified due to the presence of a previously unknown subspecies. Based on the initial results, revised investigational Dried Overnight Gram-Positive Identification panels (CPID-2) were prepared and tested. The CPID-2 panels identified 85 to 95% of S. epidermidis strains, 76 to 86% of S. hominis strains, and 88 to 92% of S. haemolyticus strains with high probability (>85%) and, overall, represented a significant improvement over the other panels for identification of these staphylococcal species.

scholarly journals Activity of a New Oral Streptogramin, XRP2868, against Gram-Positive Cocci Harboring Various Mechanisms of Resistance to Streptogramins

2006 ◽  
Vol 50 (1) ◽  
pp. 237-242 ◽  
Author(s):  
Michel Dupuis ◽  
Roland Leclercq
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bactericidal Effect ◽  
The Other ◽  
Mechanisms Of Resistance ◽  
Coagulase Negative Staphylococci ◽  
Gram Positive ◽  
Strong Activity ◽  
Factor B ◽  
Gram Positive Cocci

ABSTRACT The antibacterial activity of XRP2868, a new oral streptogramin composed of a combination of RPR132552 (streptogramin A) and RPR202868 (streptogramin B), was evaluated against a collection of clinical gram-positive isolates with characterized phenotypes and genotypes of streptogramin resistance. The effects of genes for resistance to streptogramin A or B on the activity of XRP2868 and its components were also tested by cloning these genes individually or in various combinations in gram-positive recipient strains susceptible to quinupristin-dalfopristin. The species tested included Staphylococcus aureus, coagulase-negative staphylococci, Enterococcus faecalis, Enterococcus faecium, Streptococcus pneumoniae, and other species of streptococci. XRP2868 was generally fourfold more potent than quinupristin-dalfopristin against S. aureus, E. faecium, and streptococci and had activity against E. faecalis (MICs = 0.25 to 1 μg/ml). XRP2868 appeared to be affected by the same mechanisms of resistance as those to quinupristin-dalfopristin. Nevertheless, the strong activity of factor A of the oral streptogramin enabled the combination to be very potent against streptogramin-susceptible staphylococci, streptococci, and E. faecium (MICs = 0.03 to 0.25 μg/ml) and to retain low MICs against the strains harboring a mechanism of resistance to factor A or factor B of the streptogramin. However, the combination of mechanisms of resistance to factors A and B caused an increase in the MICs of XRP2868, which reached 1 to 4 μg/ml. As with the other streptogramins, there was a reduction in the bactericidal effect of XRPR2868 when the staphylococcal strains acquired a constitutively expressed erm gene.

scholarly journals Activities of Dalbavancin against a Worldwide Collection of 81,673 Gram-Positive Bacterial Isolates

2009 ◽  
Vol 53 (3) ◽  
pp. 1260-1263 ◽  
Author(s):  
Douglas J. Biedenbach ◽  
Jan M. Bell ◽  
Helio S. Sader ◽  
John D. Turnidge ◽  
Ronald N. Jones
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Isolates ◽  
Coagulase Negative Staphylococci ◽  
Gram Positive ◽  
Resistant Strains ◽  
Vancomycin Resistant ◽  
Long Acting

ABSTRACT Dalbavancin, a long-acting lipoglycopeptide, was evaluated against 81,673 isolates of staphylococci, enterococci, and streptococci collected from 33 countries during worldwide resistance surveillance (2002 to 2007). Regardless of susceptibility to oxacillin, comparable potencies for dalbavancin against Staphylococcus aureus and coagulase-negative staphylococci from all countries were noted (MIC90, 0.06 to 0.12 μg/ml). Vancomycin-susceptible Enterococcus spp. had dalbavancin MIC90s comparable to those for staphylococci, whereas vancomycin-resistant strains were more resistant (MIC50, >4 μg/ml). β-Hemolytic and viridians group streptococci were very susceptible to dalbavancin (MIC90, ≤0.03 μg/ml). Overall, dalbavancin was ≥16-fold more active than vancomycin against the monitored gram-positive species.

scholarly journals Characterization of Some Commercially Important Octopus (Mollusca: Cephalopoda) from Indonesian Waters using Mitochondrial DNA Cytochrome Oxidase Sub-Unit I (Mt-DNA COI)

2021 ◽  
Vol 26 (1) ◽  
pp. 17-26
Author(s):  
Nenik Kholilah ◽  
Norma Afiati ◽  
Subagiyo Subagiyo
Keyword(s):  
Species Identification ◽  
Dna Sequences ◽  
Pcr Amplification ◽  
Species Level ◽  
The Other ◽  
Mt Dna ◽  
Commercially Important ◽  
The Difference ◽  
Coi Sequences

As per the FAO data, octopus identification is very limited in the species level at world fishery and also they are cryptic nature. On the other hand, Indonesia is one of the top ten highest octopus exporters. This study therefore aimed to determine the species of octopus based on phylogenetic analysis of mt-DNA COI. Octopuses were collected from nine different locations throughout Indonesia, i.e., Anambas, Bangka-Belitung, Cirebon, Karimunjawa, Tuban, Lombok, Buton, Wakatobi and Jayapura. Samples were mostly in the form of tentacles that were directly collected from fishermen. After being preserved in 96% ethanol, the sample was extracted in 10% chelexÒ, PCR amplification using Folmer’s primer then was further analysed by sequencing in Sanger methods. Of the 24 samples sequenced, the results recognized four species Octopodidae belongs to the three genera, named Amphioctopus aegina, Hapalochlaena fasciata, Octopus laqueus and Octopus cyanea. Mean pair-wise distances of within-species were ranged from 0 to 5.5 % and between-species was ranged from 12.9 to 15.8 %. This study distinctly confirmed the difference between genus Amphioctopus and Hapalochlaena (15.5 %), as also between O. laqueus and O. cyanea (12.9%) which was previously not completely distinguished. Although performing species identification using DNA sequences for shallow-water benthic octopus species is perhaps considered premature, this study indicated the possible application of COI sequences for species identification, thereby providing a preliminary dataset for future DNA barcoding of octopus, in particular for Indonesia waters.

scholarly journals In Vitro Activity of HSR-903, a New Oral Quinolone, against Bacteria Causing Respiratory Infections

1999 ◽  
Vol 43 (7) ◽  
pp. 1767-1768 ◽  
Author(s):  
Akira Watanabe ◽  
Yutaka Tokue ◽  
Hiroshi Takahashi ◽  
Tohru Kikuchi ◽  
Takao Kobayashi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Respiratory Infections ◽  
The Other ◽  
Vitro Activity ◽  
Respiratory Pathogens ◽  
In Vitro Activity ◽  
Gram Negative ◽  
Resistant Strains

ABSTRACT The in vitro activity of HSR-903, an oral quinolone, against 196 recent clinical isolates of respiratory pathogens was evaluated. HSR-903 was 2 to 32 times more active than ofloxacin, ciprofloxacin, and sparfloxacin against Staphylococcus aureus, including methicillin-resistant strains, and Streptococcus pneumoniaeand was at least as active as the other quinolones against gram-negative pathogens.

scholarly journals Resistance to Antibiotics in Strains of Staphylococcus spp., Enterococcus spp. and Escherichia coli Isolated from Rectal Swabs of Pigs

2008 ◽  
Vol 77 (1) ◽  
pp. 103-110 ◽  
Author(s):  
M. Kolář ◽  
J. Bardoň ◽  
I. Vágnerová ◽  
P. Sauer ◽  
D. Koukalová ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Pilot Project ◽  
Resistant Bacteria ◽  
Coagulase Negative Staphylococci ◽  
Microdilution Method ◽  
Rectal Swabs ◽  
Resistant Strains

The study aimed at determining the level of resistance of selected bacterial species (Staphylococcus spp., Enterococcus spp., Escherichia coli) isolated from rectal swabs of pigs to antimicrobial agents. The tested strains were isolated from piglets aged 7 to 30 days. Bacterial species were identified by standard microbiological techniques and susceptibility to antibiotics was determined quantitatively by the standard microdilution method. Resistance of the Staphylococcus aureus strain to oxacillin was confirmed by detection of the mecA gene and PBP2a. A total of 115 Staphylococcus spp. isolates were collected. In the case of Staphylococcus aureus, the methicillin-resistant strain (MRSA) was identified. Moreover, higher frequency of coagulase-negative staphylococci with minimum inhibitory concentration of oxacillin ≥ 0.5 mg/l was noticed. Inducible resistance to clindamycin in the Staphylococcus hominis strain was also detected. The strains of Enterococcus spp. (61 isolates) exhibited high resistance to tetracycline (98.5%), erythromycin (86.8%) and chloramphenicol (54.4%). Vancomycin-resistant enterococci were not isolated. In the case of Escherichia coli strains (111 isolates), higher frequency of resistant strains to tetracycline (81.1%) and ampicillin (62.2%) was documented. Resistance to fluoroquinolones and production of broad-spectrum β-lactamases was not noticed. The presented study may be considered as a pilot project assessing the prevalence of resistant bacteria in piglets kept on a single farm. It demonstrated the presence of resistant strains of Staphylococcus spp., including one MRSA strain, Enterococcus spp. and Escherichia coli. These strains may be present as a result of postnatal colonization with both bacterial microflora of dams and environmental microflora.

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