scholarly journals Criteria for measuring the efficacy of trachoma vaccines in baboons

1971 ◽  
Vol 69 (4) ◽  
pp. 717-725
Author(s):  
L. H. Collier ◽  
Elaine Lightman
Keyword(s):  
Clinical Signs ◽  
Therapeutic Agents ◽  
Control Measures ◽  
Pathogenic Strain ◽  
Challenge Dose ◽  
Subsequent Challenge ◽  
The Mean ◽  
And Control ◽  
Inflammation Score ◽  
Better Than

SUMMARYTrachoma vaccines are usually assayed by testing their ability to protect monkeys or baboons against subsequent challenge of the conjunctiva with a pathogenic strain of trachoma/inclusion conjunctivitis (TRIC) agent. In such experiments the course of infection in vaccinated baboons was compared in terms of arbitrary scores assigned to a range of clinical signs, and of counts of TRIC inclusions in conjunctival scrapings. Analysis of many such scores indicated that after a large challenge dose of strain MRC-4s, the scores for signs of inflammation reached their maximum earlier than the follicle score; the inflammation score was closely related to the number of inclusions, whereas the follicle score was not. With this system, the optimum periods for eliciting differences between vaccinated and control measures varied according to the sign used; it was later for follicles than for inflammation or inclusions. For assessing the influence of vaccination, the mean of the inflammation scores read weekly for the first 3 weeks after challenge and the mean inclusion score over the same period were equally satisfactory, and either was rather better than the mean of three follicle scores taken over the period 3–6 weeks.For assessing the influence of vaccines or therapeutic agents on experimental trachoma it is important to determine which signs discriminate best between treated and control animals, and the optimum times for measuring them.

scholarly journals An afebrile heartwater-like syndrome in goats

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Rhoda Leask ◽  
Kenneth P. Pettey ◽  
Gareth F. Bath
Keyword(s):  
Clinical Signs ◽  
Control Measures ◽  
Sub Saharan Africa ◽  
Limiting Factor ◽  
Febrile Reaction ◽  
Presumptive Diagnosis ◽  
Appropriate Treatment ◽  
Sub Saharan ◽  
Characteristic Features ◽  
And Control

Heartwater is a serious limiting factor for sheep and goat production in the major endemic area of sub-Saharan Africa and therefore most knowledge, research and control methods originate from this region. Whilst the usual or common clinical presentations can be used to make a presumptive diagnosis of heartwater with a good measure of confidence, this is not always the case, and animals suffering from heartwater may be misdiagnosed because their cases do not conform to the expected syndrome, signs and lesions. One aberrant form found occasionally in the Channel Island breeds of cattle and some goats is an afebrile heartwaterlike syndrome. The most constant and characteristic features of this heartwater-like syndrome comprise normal temperature, clinical signs associated with generalised oedema, and nervous signs, especially hypersensitivity. The presumption that the disease under investigation is the afebrile heartwater-like syndrome entails a tentative diagnosis based on history and clinical signs and the response to presumed appropriate treatment (metadiagnosis). The afebrile heartwater-like syndrome presents similarly to peracute heartwater but without the febrile reaction. Peracute cases of heartwater have a high mortality rate, enabling confirmation of the disease on post-mortem examination. Recognition of the afebrile heartwater-like syndrome is important to prevent deaths and identify the need for appropriate control measures.

scholarly journals The Effect of Using Word Family Game on The Vocabulary Mastery of The Fifth Grade Students at SD Negeri 1 Astina in Academic Years of 2017/2018

2017 ◽  
Vol 1 (3) ◽  
pp. 122
Author(s):  
Ni Komang Amelia Kartadi
Keyword(s):  
Fifth Grade ◽  
Control Group ◽  
Control Group Design ◽  
Fifth Grade Students ◽  
Conventional Teaching ◽  
Post Test ◽  
The Mean ◽  
And Control ◽  
Experimental Group ◽  
Better Than

This study aimed at investigating of the effect of using word family game committed by the fifth grade student in SD Negeri 1 Astina in mastering the English vocabulary. This study was True-Experimental with post-test only control group design. The population was 59 students of the fifth grade and the sample was selected by using cluster random sampling. The sample of this research study was 22 students in 5A as an Experimental Group while 22 students in 5B as a Control Group. The experimental group was taught by using Word Family Game and control group was taught without using Word Family Game. The data was analyzed descriptively and inferentially through SPSS 16.0 Program. Descriptively, the students in experimental group were achieved better than the students in control group. It was proven by the result of the mean score of the experimental group was 90.45, while the mean score of control group was 83.18. The result of the t-test also showed that the score of the (tobs) was 3.393 which the score of (tcv) was 1.682 which based on the degree of freedom was 42. It showed that the (tobs) > (tcv), where: 3.393 > 1.682. It could be concluded that the word family game had a significant effect on the students’ vocabulary mastery rather than using conventional teaching. 

scholarly journals Oral Administration of Lactobacillus Strains Isolated from Breast Milk as an Alternative for the Treatment of Infectious Mastitis during Lactation

2008 ◽  
Vol 74 (15) ◽  
pp. 4650-4655 ◽  
Author(s):  
E. Jiménez ◽  
L. Fernández ◽  
A. Maldonado ◽  
R. Martín ◽  
M. Olivares ◽  
...  
Keyword(s):  
Breast Milk ◽  
Clinical Signs ◽  
Control Group ◽  
Control Groups ◽  
Lactobacillus Salivarius ◽  
Probiotic Group ◽  
Efficient Alternative ◽  
The Mean ◽  
And Control ◽  
Infectious Mastitis

ABSTRACT In this study, 20 women with staphylococcal mastitis were randomly divided in two groups. Those in the probiotic group daily ingested 10 log10 CFU of Lactobacillus salivarius CECT5713 and the same quantity of Lactobacillus gasseri CECT5714 for 4 weeks, while those in the control one only ingested the excipient. Both lactobacillus strains were originally isolated from breast milk. On day 0, the mean staphylococcal counts in the probiotic and control groups were similar (4.74 and 4.81 log10 CFU/ml, respectively), but lactobacilli could not be detected. On day 30, the mean staphylococcal count in the probiotic group (2.96 log10 CFU/ml) was lower than that of the control group (4.79 log10 CFU/ml). L. salivarius CECT5713 and L. gasseri CECT5714 were isolated from the milk samples of 6 of the 10 women of the probiotic group. At day 14, no clinical signs of mastitis were observed in the women assigned to the probiotic group, but mastitis persisted throughout the study period in the control group women. In conclusion, L. salivarius CECT5713 and L. gasseri CECT5714 appear to be an efficient alternative for the treatment of lactational infectious mastitis during lactation.

Satisfaction and performance in patient selected goals after grip reconstruction in tetraplegia

2010 ◽  
Vol 35 (7) ◽  
pp. 563-568 ◽  
Author(s):  
J. Wangdell ◽  
J. Fridén
Keyword(s):  
Upper Extremity ◽  
Daily Life ◽  
Performance Measure ◽  
Occupational Performance ◽  
Canadian Occupational Performance Measure ◽  
Basic Activity ◽  
The Mean ◽  
And Performance ◽  
And Control ◽  
Better Than

Reconstruction of grip in tetraplegia aims to improve upper extremity performance and control in daily life. We evaluated the effects of surgery and rehabilitation on performance and satisfaction of patient identified activity goals in 20 patients (22 arms) who had grip reconstructions for both finger and thumb flexion. Patients assessed an improvement in both performance and satisfaction after surgery in all groups of activities assessed using the Canadian Occupational Performance Measure (COPM). The mean improvement at 6 and 12 months was 3.5 points better than the 2.5 points before surgery. Before surgery 36% of the goals identified were impossible to perform. After surgery, 78% of these goals were possible. The largest improvement was observed in the basic activity of ‘eating’ but significant improvement was also noted in activities generally regarded as complex and not measured in standard ADL such as ‘doing housework’ and taking part in ‘leisure’.

scholarly journals The Diagnostic Challenges of Ovine Pulmonary Adenocarcinoma

Ruminants ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 58-71
Author(s):  
Hélder Quintas ◽  
Isabel Pires ◽  
Andreia Garcês ◽  
Justina Prada ◽  
Filipe Silva ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Pulmonary Adenocarcinoma ◽  
Diagnostic Methods ◽  
Control Measures ◽  
Economic Losses ◽  
Ovine Pulmonary Adenocarcinoma ◽  
X Ray ◽  
Club Cells ◽  
Pulmonary Adenomatosis ◽  
And Control

Ovine pulmonary adenocarcinoma (OPA), also known as sheep pulmonary adenomatosis and jaagsiekte, is a contagious pulmonary tumor of sheep, characterized by neoplastic proliferation of type II pneumocyte and club cells. OPA is induced by the oncogenic activity of the envelope glycoprotein (Env) of exogenous jaagsiekte sheep retrovirus (JSRV). This disease is associated with significant economic losses in numerous sheep raising countries. The onset of suggestive clinical signs is often late, making difficult the early diagnosis of the disease and timely implementation of control measures on the affected farms. Further, the lack of diagnostic tests that can be performed routinely by veterinary clinicians to accurately assess infected animals (e.g., serological or others) means that the true prevalence at flock level is not known. Imaging diagnostic methods (e.g., ultrasound, X-ray and computed tomography) can be used to support the clinical diagnosis, even in pre-clinical stages in affected flocks. The diagnosis must be confirmed by PCR of nasal excretions or immunohistochemistry and PCR of tumor lesions. No vaccine for OPA has yet been developed. Thus, in this work, we review the main methods of diagnosis of OPA in order to support the clinician in the identification of the disease, avoid underdiagnosis and allow the implementation of suitable measures to prevent and control its spread.

Sonography and Test-Feeding in the Diagnosis of Infantile Hypertrophic Pyloric Stenosis

2020 ◽  
Vol 37 (1) ◽  
pp. 25-30
Author(s):  
Syed Amir Gilani ◽  
Raham Bacha ◽  
Iqra Manzoor
Keyword(s):  
Pyloric Stenosis ◽  
Hypertrophic Pyloric Stenosis ◽  
Clinical Signs ◽  
Muscle Thickness ◽  
Male Gender ◽  
Infantile Hypertrophic Pyloric Stenosis ◽  
Study Duration ◽  
The Mean ◽  
Pyloric Muscle ◽  
Better Than

Objective: To determine the use of sonography and test-feeding in the diagnosis of infantile hypertrophic pyloric stenosis (IHPS). Methodology: A total of 79 infants from 2 weeks up to 12 weeks presenting with clinical signs (projectile vomiting) of IHPS were included in the study while sent to sonography. The study duration was February 2014 to December 2019. Results: The mean age of patients was 5.18 ± 2.69 weeks ranging from 1 to 12 weeks. Male gender was predominant with 62 (78.5%) cases. Of the total 79 cases, 36 (54.6%) were suggested as positive cases of IHPS and 43 (54.4%) were as normal with sonography. Most of the cases of IHPS (23; 29.1%) were observed in firstborn babies. Mean pyloric length in normal and IHPS was 12.69 ± 0.87 and 20.78 ± 2.28 mm, respectively, and mean pyloric muscle thickness in the normal and IHPS was 2.24 ± 0.36 and 5.27 ± 0.82 mm, respectively, while mean pyloric diameter in normal and IHPS was 12.69 ±0.87 and 15.01± 2.52 mm, respectively. Conclusion: The reliability of sonographic findings was better than test-feeding in the diagnosis of IHPS. Male infants, in this cohort, were five times more susceptible to develop IHPS, compared with females.

Extracapillary Proliferative Glomerulonephritis in Children

PEDIATRICS ◽  
1975 ◽  
Vol 56 (3) ◽  
pp. 434-442
Author(s):  
Sudhir K. Anand ◽  
Carl W. Trygstad ◽  
Hari M. Sharma ◽  
James D. Northway
Keyword(s):  
Streptococcal Infection ◽  
Clinical Course ◽  
Renal Involvement ◽  
Therapeutic Agents ◽  
Proliferative Glomerulonephritis ◽  
The Mean ◽  
Histopathologic Changes ◽  
Better Than

The clinical course and renal pathology of 17 children with acute extracapillary proliferative glomerulonephritis is reported. Patients with systemic ses associated with renal involvement were excluded. The onset followed streptococcal infection in ten; of these, four have died, one has been transplanted, and the remaining five have completely healed. The mean follow-up of the latter five patients was 32 months (range, 18 to 57 months). Of the other seven patients without evidence of preceding streptococcal infection, two have died, two have been transplanted, and the remaining three all have hypertension, proteinuria, and reduced creatinine clearance. The mean follow-up of the latter three patients was 29 months (range, 14 to 38 months). The initial renal histopathologic changes and their progress in later renal biopsies is described. The role of various therapeutic agents is discussed. The prognosis in acute extracapillary proliferative glomerulonephritis following streptococcal infection appears to be better than in ones without preceding streptococcal infection.

A Pilot Study of Selective Lipopolysaccharide Adsorption and Coupled Plasma Filtration and Adsorption in Adult Patients with Severe Sepsis

2015 ◽  
Vol 39 (1-3) ◽  
pp. 210-217 ◽  
Author(s):  
Michail Yaroustovsky ◽  
Marina Abramyan ◽  
Nickolay Krotenko ◽  
Dmitry Popov ◽  
Marina Plyushch ◽  
...  
Keyword(s):  
Severe Sepsis ◽  
Standard Therapy ◽  
Clinical Signs ◽  
Oxygenation Index ◽  
Control Group ◽  
Combined Use ◽  
Plasma Filtration ◽  
Extracorporeal Therapy ◽  
The Mean ◽  
And Control

Aim: To evaluate the safety and effectiveness of combined extracorporeal therapy in patients with severe sepsis after cardiac surgery. Materials and Methods: Twenty patients received combined extracorporeal therapy (LPS-adsorption with Toraymyxin columns + CPFA). The inclusion criteria were clinical signs of severe sepsis, EAA = 0.6, and PCT >2 ng/ml. 20 comparable patients in the control group received only standard therapy. Results: Each patient in the study group received 2 daily treatments of combined extracorporeal therapy. In contrast to controls, we noted an increase in the values of MAP from 73 to 82 mm Hg, (p < 0.001) and the mean oxygenation index (from 180 to 246, p < 0.001), decrease of EAA from 0.77 to 0.55, p < 0.001, and PCT (from 6.23 to 2.83 ng/ml, p < 0.001). The 28-day survival rate was 65 and 35% in the study and control groups respectively, p = 0.11. Conclusion: The combined use of LPS-adsorption and CPFA in a single circuit with standard therapy is a safe and possibly effective adjunctive method for treating severe sepsis.

scholarly journals Outbreak investigation and control case report of brucellosis: Experience from livestock research centre, Mpwapwa, Tanzania

2014 ◽  
Vol 81 (1) ◽  
Author(s):  
Gabriel M. Shirima ◽  
Seleman N. Masola ◽  
Obeid N. Malangu ◽  
Brant A. Schumaker
Keyword(s):  
Clinical Signs ◽  
Dairy Farms ◽  
Control Measures ◽  
Research Centre ◽  
Confirmatory Test ◽  
Medical Attention ◽  
Enzyme Linked Immunosorbent Assay ◽  
And Control ◽  
The Rose ◽  
S19 Vaccine

Brucellosis screening was conducted between 2005 and 2010 at the National Livestock Research Institute headquarters, Mpwapwa, Tanzania, following an abortion storm in cattle. The initial screening targeted breeding herds; 483 cattle were screened using the Rose Bengal Plate Test (RBPT) followed by the Competitive Enzyme-linked Immunosorbent Assay (c-ELISA) as a confirmatory test. The seropositivity on c-ELISA was 28.95% in 2005; it subsequently declined to 6.72%, 1.17%, 0.16% and 0.00% in 2006, 2007, 2009 and 2010, respectively. Brucella seropositivity was not detected in goats. Seropositivity declined following institution of stringent control measures that included: gradual culling of seropositive animals through slaughter; isolation and confinement of pregnant cows close to calving; proper disposal of placentas and aborted foetuses; the use of the S19 vaccine; and restricted introduction of new animals. It was thought that the source of this outbreak was likely to have been from the introduction of infected animals from another farm. Furthermore, humans were found with brucellosis antibodies. Out of 120 people screened, 12 (10%) were confirmed seropositive to brucella antigen exposure by c-ELISA analysis. The majority of the seropositive individuals (80%) were milkers and animal handlers from the farm. Nine individuals had clinical signs suggestive of brucellosis. All cases received medical attention from the district hospital. This achievement in livestock and human health showed that it is possible to control brucellosis in dairy farms, compared to pastoral and agro-pastoral farms, thus providing evidence to adopt these strategies in dairy farms thought to be at risk.

scholarly journals A U.S. isolate of Theileria orientalis, Ikeda genotype, is transmitted to cattle by the invasive Asian longhorned tick, Haemaphysalis longicornis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Kelcey D. Dinkel ◽  
David R. Herndon ◽  
Susan M. Noh ◽  
Kevin K. Lahmers ◽  
S. Michelle Todd ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Clinical Signs ◽  
Adult Tick ◽  
Control Measures ◽  
Asia Pacific ◽  
Haemaphysalis Longicornis ◽  
Cattle Industry ◽  
Theileria Orientalis ◽  
And Control ◽  
The U.S

Abstract Background Theileria orientalis is a tick-borne hemoparasite that causes anemia, ill thrift, and death in cattle globally. The Ikeda strain of T.orientalis is more virulent than other strains, leading to severe clinical signs and death of up to 5% of affected animals. Within the Asia–Pacific region, where it affects 25% of Australian cattle, T.orientalis Ikeda has a significant economic impact on the cattle industry. In 2017, T.orientalis Ikeda was detected in a cattle herd in Albermarle County, Virginia, United States. Months earlier, the U.S. was alerted to the invasion of the Asian longhorned tick, Haemaphysalis longicornis, throughout the eastern U.S. Abundant H.longicornis ticks were identified on cattle in the T.orientalis-affected herd in VA, and a subset of ticks from the environment were PCR-positive for T.orientalis Ikeda. A strain of T.orientalis from a previous U.S. outbreak was not transmissible by H.longicornis; however, H.longicornis is the primary tick vector of T.orientalis Ikeda in other regions of the world. Thus, the objective of this study was to determine whether invasive H.longicornis ticks in the U.S. are competent vectors of T.orientalis Ikeda. Methods Nymphal H.longicornis ticks were fed on a splenectomized calf infected with the VA-U.S.-T.orientalis Ikeda strain. After molting, a subset of adult ticks from this cohort were dissected, and salivary glands assayed for T.orientalis Ikeda via qPCR. The remaining adult ticks from the group were allowed to feed on three calves. Calves were subsequently monitored for T.orientalis Ikeda infection via blood smear cytology and PCR. Results After acquisition feeding on a VA-U.S.-T.orientalis Ikeda-infected calf as nymphs, a subset of molted adult tick salivary glands tested positive by qPCR for T.orientalis Ikeda. Adult ticks from the same cohort successfully transmitted T.orientalis Ikeda to 3/3 naïve calves, each of which developed parasitemia reaching 0.4–0.9%. Conclusions Our findings demonstrate that U.S. H.longicornis ticks are competent vectors of the VA-U.S.-T.orientalis Ikeda strain. This data provides important information for the U.S. cattle industry regarding the potential spread of this parasite and the necessity of enhanced surveillance and control measures.

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