Pathology of the eustachian tube in otitis media: an electron microscopic study

1993 ◽  
Vol 107 (7) ◽  
pp. 651-655 ◽  
Author(s):  
Samy Elwany
Keyword(s):  
Mast Cells ◽  
Eustachian Tube ◽  
Middle Ear ◽  
Inflammatory Process ◽  
Morphological Changes ◽  
Goblet Cells ◽  
Ciliated Cells ◽  
Electron Microscopic ◽  
Temporal Bones ◽  
The Relationship

The ultrastructure of the mucosa of the eustachian tube was studied in four temporal bones showing tympanosclerosis, cholesteatoma, otitic meningitis and a grafted tympanic membrane (tympanoplasty). The mucosa of tube was abnormal in the four cases confirming the relationship between the state of the eustachian tube and the inflammatory process in the middle ear. The observed abnormalities included: ciliary loss, abnormal ciliary morphology and motility, oedema of the microvilli, hyperplasia of the goblet cells and the seromucinous acini, desquamation of the non-ciliated cells and appearance of mast cells in the lamina propria of the tube. Ciliary changes were the most frequent abnormalities and the morphological changes, in general, were fewest in the case of healed tympanoplasty. The pathophysiology of the morphological changes was discussed and correlated with the disease in the middle ear.

Goblet Cell Population in the Pathological Middle Ear and Eustachian Tube of Children and Adults

1977 ◽  
Vol 86 (2) ◽  
pp. 209-218 ◽  
Author(s):  
Mirko Tos ◽  
K. Bak-Pedersen
Keyword(s):  
Cell Density ◽  
Eustachian Tube ◽  
Middle Ear ◽  
Goblet Cell ◽  
Newborn Infants ◽  
Goblet Cells ◽  
Secretory Otitis Media ◽  
Temporal Bones ◽  
Different Parts ◽  
Goblet Cell Density

On 64 temporal bones from slightly abnormal and pathological prematures and newborn infants, children, and adults, 35,000–40,000 counts of goblet cells were done in different parts of the Eustachian tube and middle ear. The goblet cells were present in all Eustachian tubes and middle ear areas. In ears previously exposed to infection the density was slightly increased in the tubal orifice and in most parts of the middle ear. In subacute and acute pathological actions, especially tubal occlusion, and in chronic diseases, such as active chronic otitis and in secretory otitis media, the goblet-cell density is appreciably increased.

Goblet Cell Population in the Normal Middle Ear and Eustachian Tube of Children and Adults

1976 ◽  
Vol 85 (2_suppl) ◽  
pp. 44-50 ◽  
Author(s):  
Mirko Tos ◽  
K. Bak-Pedersen
Keyword(s):  
Eustachian Tube ◽  
Middle Ear ◽  
Round Window ◽  
Newborn Infants ◽  
Lateral Wall ◽  
Goblet Cells ◽  
Oval Window ◽  
Mastoid Process ◽  
Temporal Bones ◽  
Age Range

On 54 temporal bones from entirely normal prematures and newborn infants, children, and adults, 30,000 to 90,000 counts of goblet cells were done in different parts of the Eustachian tube and middle ear. In the Eustachian tube, normal prematures showed a very low density of goblet cells in all localities, increasing in the pharyngeal parts gradually through childhood and reaching in the normal adult a very high density in the pharyngeal orifice. There are no differences in density between the medial and lateral wall, but in some parts a decrease of density towards the tubal roof. In the middle ear the density is low in normal prematures, increasing slightly in infants, and decreasing again during the age range 7–14 years and in adults. Goblet cells are present in all middle-ear localities, but in markedly decreasing density in this sequence: hypotympanum anteriorly, posteriorly, promontory anteriorly, in the middle, epitympanum, niche of oval window, antrum, niche of round window, mastoid process, incus, and promontory posteriorly.

Difference in Attachment of the Tensor Veli Palatini Muscle to the Eustachian Tube Cartilage with Age

2003 ◽  
Vol 112 (5) ◽  
pp. 439-443 ◽  
Author(s):  
Chiaki Suzuki ◽  
Isamu Sando ◽  
Miwa Kitagawa ◽  
Carey D. Balaban ◽  
Kenji Takasaki
Keyword(s):  
Eustachian Tube ◽  
Later Life ◽  
Measurement Methods ◽  
3 Dimensional ◽  
Dimensional Reconstruction ◽  
Tensor Veli Palatini Muscle ◽  
Normal Human ◽  
Development And Aging ◽  
Temporal Bones ◽  
The Relationship

To test a hypothesis that ventilation of the eustachian tube (ET) varies with age, we investigated the relationship between age and the attachment of the tensor veli palatini muscle (TVPM) to the lateral lamina of the ET cartilage in 12 normal human temporal bones obtained from individuals 3 months to 81 years old. We used computer-aided 3-dimensional reconstruction and measurement methods. We found that the length of the TVPM attachment and its ratio to the length of the ET, especially that of the cartilaginous portion of the ET, increases with age from infancy to adulthood, and decreases with age from young adulthood to later life. These findings are thought to be related to postnatal development and aging. The possibility of differences in ventilation function with age is discussed.

Regenerated Middle Ear Mucosa after Tympanoplasty. Part I. Transmission Electron Microscopy

1986 ◽  
Vol 94 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Roberto Gamoletti ◽  
Paola Poggi ◽  
Mario Sanna ◽  
Carlo Zini
Keyword(s):  
Electron Microscopy ◽  
Middle Ear ◽  
Goblet Cells ◽  
Secretory Cells ◽  
Canal Wall ◽  
Ciliated Cells ◽  
Middle Ear Mucosa ◽  
Morphologic Characteristics ◽  
Transmission Electron ◽  
Ultrastructural Appearance

The ultrastructural appearance of the regenerated middle ear mucosa—found at the second operation of staged intact canal wall tympanoplasty (ICWT) with mastoidectomy—has been evaluated with the transmission electron microscope. The regenerated epithelium showed all the morphologic characteristics of the normal middle ear mucosa: ciliated cells, noncillated cells, and secretory cells. All of these (Including goblet cells) have been found in the specimens. It is concluded that a normal middle ear mucosa regenerates to cover all denuded bone surfaces after the first operation of staged ICWT with mastoidectomy, when silicone rubber sheeting has been used to prevent adhesions and maintain an air-containing middle ear space.

Narrowest (ISTHMUS) Portion of Eustachian Tube: A Computer-Aided Three-Dimensional Reconstruction and Measurement Study

1997 ◽  
Vol 106 (7) ◽  
pp. 583-588 ◽  
Author(s):  
Masaharu Sudo ◽  
Isamu Sando ◽  
Akihiro Ikui ◽  
Chiaki Suzuki
Keyword(s):  
Eustachian Tube ◽  
Middle Ear ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Cross Sectional ◽  
Computer Aided ◽  
Dimensional Reconstruction ◽  
Tensor Veli Palatini Muscle ◽  
Normal Human ◽  
Temporal Bones

Nine normal human temporal bones from persons 16 to 88 years old were studied by computer aided three-dimensional reconstruction and measurement. The length of the eustachian tube (ET) lumen in three portions (from pharyngeal orifice to tympanic orifice: cartilaginous, junctional, and bony) averaged 23.6 ±4.3 mm, 3.0 ± 1.9 mm, and 6.4 ± 2.6 mm. The narrowest portion of the ET lumen was in the cartilaginous portion in all cases: 20.5 ± 4.2 mm from the pharyngeal orifice and 3.1 ± 1.6 mm from the pharyngeal margin of the junctional portion. The cross-sectional area of the narrowest portion was 0.65 ± 0.2 mm2. The tendon of the tensor veli palatini muscle (TVPM) inserted into the lateral lamina in the narrowest portion of the ET lumen in five of nine cases. These results suggest that contraction of the TVPM opens the narrowest portion of the ET lumen to ventilate the middle ear and that this portion also plays a role in protecting the middle ear.

Distribution and Density of Goblet Cells in the Normal Adult Human Middle Ear

1973 ◽  
Vol 82 (2) ◽  
pp. 240-247 ◽  
Author(s):  
K. Bak-Pedersen ◽  
M. Tos
Keyword(s):  
Cell Density ◽  
Middle Ear ◽  
Goblet Cell ◽  
Anterior Part ◽  
Goblet Cells ◽  
Mastoid Process ◽  
Clinically Normal ◽  
Normal Series ◽  
Temporal Bones ◽  
Goblet Cell Density

The purpose of this study was to map the distribution and density of goblet cells in the clinically normal middle ear. From 12 temporal bones, derived from 12 adult patients with clinically normal middle ears, the mucosa was prepared and stained by the PAS-alcian blue whole-mount method. In six of the patients the middle ears with meticulous gross and microscopic investigation showed mild sequelae of previous otitis media, called the slightly abnormal series, whereas the other six patients who had entirely normal middle ears were designated the normal series. Each middle ear was investigated in 12 different areas: tympanic orifice, hypotympanum anteriorly and posteriorly, round window niche, oval window niche, promontory anteriorly, in the middle, and posteriorly, epitympanum, antrum, and mastoid process. In each middle ear area the goblet-cell density and distribution were determined on the basis of 30 to 50 counts of 0.01768 mm2 epithelial surface, a total of at least 4800 counts. In six patients goblet cells were found in all 12 areas, in three patients in 11, and in three patients in 10. In both series there was a distinct decrease in goblet-cell density from the tympanic orifice, towards the posterior areas of the middle ear and the mastoid process. However, two patients of the slightly abnormal series exhibited a considerably greater density in the posterior than in the anterior part of the hypotympanum. The mean goblet-cell density in the slightly abnormal series was distinctly greater in all middle ear areas than in the normal series. Correspondingly, goblet cells were demonstrated in the antrum and mastoid process in all patients of the slightly abnormal series, but in only half of the normal series. Therefore, the greater goblet-cell density in the slightly abnormal series presumaby results from a previous past disease condition of the middle ear.

scholarly journals Effect of the Dexamethasone and the Difenihidramiana on the Degranulation of the Eosinophilic Granular Cell of Tilapia

2021 ◽  
pp. 20-26
Author(s):  
Luis Alberto Romano ◽  
Virgínia Fonseca Pedrosa
Keyword(s):  
Escherichia Coli ◽  
Quantitative Analysis ◽  
Mast Cells ◽  
Inflammatory Process ◽  
Granular Cell ◽  
Treated Group ◽  
Control Group ◽  
E Coli ◽  
Release Histamine ◽  
The Relationship

The aim of this study was to determine the relationship between EGC degranulation in fish injected with formalin-killed Escherichia coli and the effect of dexamethasone, diphenhydramine supplied separately and before formalin-killed E. coli. We performed a quantitative analysis of the number of cell granules and demonstrated that: compared to the EGCs of animals, the injection of dead E. coli with formalin generated degranulation of the EGC, while the administration of dexamethasone alone did not show significant differences with control group animals. The administration of diphenhydramine alone did not show significant differences neither with the animals of the dexamethasone treated group nor with those of the control group. When dexamethasone was administered one hour before the E. coli injection, degranulation was apparently inhibited and the number of granules did not show significant differences either with the animals in the control group or with those treated with dexamethasone. Finally, when this group was compared with the group of animals that were only injected with E. coli, the differences were statistically significant. However, when diphenhydramine was administered one hour before E. coli injection, a critical inhibition of EGC degranulation was evidenced, with a marked increase in the number of granules. All this seems to show that dexamethasone can partially inhibit the release of substances that participate in the inflammatory process. Diphenhydramine, a recognized antihistamine, inhibited degranulation of EGCs. These results suggest that EGC can release histamine like mammalian mast cells.

Eustachian Tube Lumen: Comparison between Normal and Inflamed Specimens

1989 ◽  
Vol 98 (8) ◽  
pp. 630-634 ◽  
Author(s):  
Jacob Sadé ◽  
Michal Luntz
Keyword(s):  
Otitis Media ◽  
Eustachian Tube ◽  
Middle Ear ◽  
Sectional Area ◽  
Secretory Otitis Media ◽  
Cross Sectional ◽  
Tube Lumen ◽  
Luminal Area ◽  
The Mean ◽  
Temporal Bones

This study presents measurements of the cross-sectional luminal area of the eustachian tube. Comparisons are made between the lumens of eustachian tubes obtained from temporal bones presenting acute or secretory otitis media and those from noninflamed temporal bones. The material consisted of 71 temporal bones obtained postmortem from individuals up to 2 years of age. Forty-six of these showed no middle ear inflammation, while 25 presented either acute or secretory otitis media. In both groups the lumens of all the eustachian tubes were patent, presenting no obstruction. The mean cross-sectional area of the lumens of inflamed temporal bones was smaller than that of the noninflamed ones. This difference was not found to be statistically significant in the cartilaginous regions and was found to be statistically significant or borderline significant in the bony parts of the eustachian tube.

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