Inhibitory effect ofHuRgene small interfering RNA segment on laryngeal carcinoma Hep-2 cell growth
AbstractObjectives:To investigate the effect of theHuRgene on laryngeal carcinoma Hep-2 cell growth, and to analyse correlations between theHuR, cyclooxygenase-2 and survivin genes.Study design:Experiment study.Setting:Department of Otolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, Ningbo, a tertiary care centre in China.Methods:Copies of a small interfering RNA segment directed against theHuRgene were transfected into Hep-2 cells using LipofectamineTM2000. The effect of the small interfering RNA segment on Hep-2 cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Changes in the expression of theHuR, cyclooxygenase-2 and survivin genes were detected by semi-quantitative reverse transcription polymerase chain reaction analysis. Concentrations of the HuR, cyclooxygenase-2 and survivin proteins were evaluated using Western blotting.Results:Expression of theHuR, cyclooxygenase-2 and survivin genes, as indicated by messenger RNA and protein levels, was suppressed by theHuRgene small interfering RNA segment in a dose-dependent manner. The proliferation indices of all treated groups were significanlty lower than those of control groups (p < 0.05).Conclusions:Impairment ofHuRgene expression, using interfering RNA technology, can significantly suppress Hep-2 cell proliferation and induce apoptosis. TheHuRgene may be an effective target for gene therapy in patients with laryngeal carcinoma.