The Upper Cortex of Parmelia Saxatilis and other Lichen Thalli

1982 ◽  
Vol 14 (1) ◽  
pp. 29-38 ◽  
Author(s):  
D. Anglesea ◽  
C. Veltkamp ◽  
G. N. Greenhalgh

AbstractA method, including treatment with an enzyme/detergent mixture, of revealing features of the cortical hyphal arrangement in lichen thalli is described. The cortex structure of Parmelia saxatilis, Xanthoria parietina, Hypogymnia physodes and Lecanora muralis is illustrated and described.

Author(s):  
Volga M. Khramchankova ◽  
◽  
Matsvei V. Matveyenkau ◽  

In vitro, the photoprotective, cytotoxic and photomodifying properties of hexane and benzene extracts from the widely distributed in Belarus lichens Cladonia arbuscula, Evernia prunastri, Hypogymnia physodes, Ramalina pollinaria and Xanthoria parietina in the culture of human keratinocytes (HaCAT), are estimated. It was found that these extracts are not photoprotective, they have a photomodifying effect upon irradiation of keratinocyte cultures with ultraviolet light. Hexane extract of E. prunastri and benzene extracts of R. pollinaria and H. physodes at a concentration of 2.5 μg/ml are photoprotectors that weaken the effect of ultraviolet radiation by 1.6 ÷ 1.8 times. Extracts of C. arbuscula and X. parietina are photosensitizers – they increase the toxic effect of ultraviolet radiation by 10 or more times, regardless of the concentration of the extract in the nutrient medium. Hexane and benzene extracts of C. arbuscula and H. physodes lichens are cytotoxic to the HaCAT culture. With an increase in the dose of ultraviolet irradiation of keratinocytes from zero to lethal values, the benzene extract of R. pollinaria acted as a photoprotector at a concentration of 2.5 μg / ml, and as a photosensitizer at higher concentrations. The hexane and benzene extracts of E. prunastri and H. physodes exhibited sensitizing properties that increased with an increase in the concentration of lichen extracts. C. arbuscula hexane extract and X. parietina benzene extract were the most potent photosensitizers.


Author(s):  
Evgeniya E. Muchnik ◽  
Nikolaj I. Zolotukhin ◽  
Nikolaj I. Degtyarev

Цель – первое лихенологическое обследование техногенных отвалов Михайловского горно-обогатительного комбината (Железногорский район, Курская область) с прилегающими природными и урбанизированными территориями. Материалы и методы. Сборы материалов проводились маршрутным методом в течение 2017 и 2019-2020 годов, камеральная обработка осуществлялась с применением общепринятых лихенологических методик. Идентифицированные образцы хранятся в гербариях Центрально-Черноземного заповедника им. проф. В. В. Алехина и Станции юных натуралистов г. Железногорска. Результаты и обсуждение. Выявленная лихенобиота включает 88 видов (преимущественно макролишайников, вследствие методики сбора) из 38 родов, 18 семейств. Наиболее распространенные, металл-толерантные виды: геоплезные Cladonia fimbriata, C. coniocraea, эпигеидные C. rei, C. mitis, C. cornuta, C. furcata, C. phyllophora и C. gracilis, эпифито-эпиксильные Evernia prunastri, Parmelia sulcata, Xanthoria parietina, Hypogymnia physodes и Physcia adscendens. Составлен аннотированный список в различной степени редких видов, среди которых 4 новых для Центрального Черноземья (Cladonia cervicornis, Dibaeis baeomyces, Usnea dasopoga, U. lapponica); 5 новых для Курской области (Bryoria fuscescens, Cladonia carneola, C. deformis, Melanohalea septentrionalis, Peltigera extenuata); 6 занесенных в Красную книгу Курской области (Cladonia subrangiformis, C. subulata, Peltigera praetextata, Platismatia glauca, Pseudevernia furfuracea и Usnea subfloridana); еще 5 редки в области или на более обширных территориях (Acarospora veronensis, Protoparmeliopsis muralis, Ramalina pollinaria, Trapelia coarctata, Usnea hirta). Сосредоточение значительного числа редких видов в техногенных ландшафтах, по-видимому, обусловлено рядом причин: наличием крупных выходов редкого в области и Центральном Черноземье субстрата (ожелезненного песчаника); слабой конкуренцией со стороны сосудистых растений на бедных и обогащенных металлами почвах и грунтах; ботанико-географическим положением территории; спецификой локального загрязнения среды, представленного преимущественно «кислыми» поллютантами и частицами металлов. Заключение. Отмечена приуроченность регионально редких кустистых и листоватых эпифитов из экологической группы ацидофилов к насаждениям березы в изученных техногенных ландшафтах. Предложены некоторые меры охраны выявленных редких видов лишайников.


2018 ◽  
Vol 55 (3) ◽  
pp. 320-324
Author(s):  
Emese Varga ◽  
Laszlo Fortuna ◽  
Daniel Lingvay ◽  
Adriana Mariana Bors ◽  
Nicoleta Oana (Butoi) Nicula ◽  
...  

By electrical determinations, digital microscopy and visual observations, the anticorrosive insulation capacity of the paint layers applied to the three sections of a bridge over the Tisza River was assessed. The visual observations have highlighted the fact that on the Eastern section repainted in 2013, biofouling increases are significant (covers up to 10 % of the surface) - both ferns have been identified (Hypogymnia physodes and Xanthoria parietina), filamentous molds (Aspergillus fumigatus and Aspergillus niger) as well as algae and moss (green - capable of photosynthesis). On paint applied in 1986, no bio fouling was visible but the painting material is aged and shows rust traces (5-10 % of the surface) and exfoliation between the paint layers (10-20 % of the surface). No bio fouling and/ or exfoliation/ degradation of the paint layer have been observed on the paint applied in 2014. Determinations of electrical insulation resistance correlated with those of the paint layers� thickness are closely related to the visual observations. The values obtained for the specific resistivity of the paint layers applied in 2013 (with bio fouling increases) are approx. 38 times lower than those applied in 2014. These findings suggest that the painting material used in 1983 for the top coat realization, showed major qualitative vices.


Author(s):  
D. V. Tapalsky ◽  
D. R. Petrenev ◽  
O. M. Khramchenkova ◽  
A. S. Doroshkevich

Aim. Study spectrum and expressiveness of antibacterial and antifungal properties oflichen extracts. Materials and methods. Antimicrobial activity of acetone extracts from Hypogymnia physodes, Xanthoria parietina, Evernia prunastri, Ramalina pollinaria, Cladonia arbuscula lichens was determined by micro-dilution methods in broth for 4 - 500 mcg/ml concentrations against 13 strains from ATCC collection and 6 clinical isolates. Results. High antibacterial activity of H. physodes and C. arbuscular extracts against staphylococci and enterococci was detected (MIC 31 - 62 mcg/ml). Antimicrobial activity against enterobacteria and Pseudomonas aeruginosa was absent for all the extracts. E.prunastri, H.physodes and C arbuscula extracts were active against Stenotrophomonas maltophilia strains (MIC 250 - 500 mcg/ml). Antifungal activity (MIC 500 mcg/ml for 4 Candida strains) was only detected for the E. prunastri extract. Conclusion. H.physodes and C. arbuscula lichens can be examined as a perspective source of antibacterial substances, effective against antibiotics resistant staphylococci, streptococci and S. maltophilia strains.


2021 ◽  
Vol 22 (1) ◽  
pp. 53-59
Author(s):  
Amer H. Tarawneh

Lichens have widely been used as bioindicators to reflect the quality of the environment. The present study was conducted to investigate the lichens diversity that grows on the surface of waste heaps from an abandoned old copper mine in Mlynky, Slovakia. In spite of the heavy metalcontaminated environment, we documented twenty species of lichens in the selected site. Taxonomically the most numerous group were represented by Cladonia with seven species, as well other species; namely, Acarospora fuscata, Cetraria islandica, Dermatocarpon miniatum, Hypogymnia physodes, Hypogymnia tubulosa, Lecanora subaurea, Lepraria incana, Physcia aipolia, Porpidia macrocarpa, Pseudevernia furfuracea, Rhizocarpon geographicum and Xanthoria parietina. The content of selected heavy metals (Cu, Fe, and Zn) in the predominant lichens Cetraria islandica, Cladonia digitata, Cladonia pyxidata, Hypogymnia physodes and Pseudevernia furfuracea were analyzed. The highest content of Cu, Fe, and Zn was found in Cladonia pyxidata collected from mine-spoil heaps with concentration 46 ± 4.4, 82.5 ± 22.6, 4.8 ± 1.6 mg/kg, respectively. Interestingly, Cladonia pyxidata collected from the forest surrounding the location showed 15 times lower concentration for Cu. Additionally, similar results were found for Fe and Zn.


1992 ◽  
Vol 67 (01) ◽  
pp. 019-027 ◽  
Author(s):  
Joseph E Addiego ◽  
Edward Gomperts ◽  
Liu Shu-Len ◽  
Patricia Bailey ◽  
Suzanne G Courter ◽  
...  

SummaryTo reduce the risk of pathogenic virus transmission associated with the therapeutic administration of plasma-derived antihemophilic factor (FVIIIc), a process utilizing anti-FVIIIc immunoaffinity chromatography to isolate FVIIIc has been developed. In addition, the starting cryoprecipitate solution has been treated with an organic solvent/detergent mixture to inactivate lipid-enveloped viruses. A final ion exchange chromatography step is used to further remove contaminants, e.g., anti-FVIIIc antibody, potentially leached with FVIIIc during the immunoaffinity step. The purified FVTII is stabilized for lyophili-zation and storage by the addition of human albumin. The monoclonal anti-FVIIIc antibody used in the immunoaffinity step of the process is not detectable in the final preparation. Viral reduction studies performed at specific steps of the process demonstrate that 11 logs of human immunodeficiency virus (HIV) and greater than 4-5 logs of other lipid-enveloped viruses are inactivated within the first 30 s of exposure to the solvent/ detergent mixture and 4-5 logs of various model viruses, e. g. Endomyocarditis virus (EMC), are physically removed during washing of the immunoaffinity column. The lyophilized product is reconstituted using sterile water in a matter of seconds.The pharmacokinetics of Hemofil® M were compared to those obtained using a standard heat-treated concentrate (Hemofil® CT) in five severe factor VIII deficient hemophiliacs in a randomized, cross-over study. No statistically significant differences were observed in mean half life (p >0.6) or median recovery (p = 0.4) between the two preparations. No clinically significant adverse effects were observed in patients receiving either FVIII preparation.In addition, 43 patients at 18 different centers underwent pharmacokinetic studies, with a nominal dose of 50 u/kg FVIIIc Hemofil® M. The mean recovery was 103.6%, and the t 1/2 was 14.6 h. The recovery of FVIII in this group was as expected, providing an increase of assayed FVIII of approximately 2% per unit of FVTII/kg infused.Clinical trials using Hemofil® M have been initiated in 124 hemophilia A patients. The safety and efficacy of Hemofil® M has been established. To date, 0 of 60 patients tested have seroconverted to HIV. None of the previously untreated patients show clinical or laboratory evidence of Non-A, Non-B hepatitis (NANB), with 21 patients remaining negative as far as presence of antibodies to the Hepatitis C virus (a-HCV negative) at least 6 months after the initial infusion. There is no evidence of neoantigenicity, evidenced by seroconversion to murine antibody. An 8.7% (2 of 23) prevalence of anti-FVIIIc inhibitor development has been observed in previously untreated patients with FVIIIc⩽3%, receiving only the monoclonally purified solvent/ detergent treated FVIII concentrate while on study and on poststudy surveillance. All patients demonstrated clinical hemostasis following product use for either on demand bleeding or surgical prophylaxis.


2019 ◽  
Vol 47 (1) ◽  
pp. 149-160
Author(s):  
E Latkowska ◽  
J. Bialczyk ◽  
M Węgrzyn ◽  
U. Erychleb

2020 ◽  
Vol 57 ◽  
pp. 3-34
Author(s):  
Ulf Schiefelbein ◽  
Frieda Engel ◽  
Franziska Masberg ◽  
Svea Lübke ◽  
Johann Schiefelbein ◽  
...  

Die Flechtendiversität und -quantität wurde in den Jahren 2017/2018 in Rostock an 53 Bäumen erfasst. Die Ergebnisse wurden mit den Ergebnissen einer Kartierung von 1994/1995 verglichen. Ferner wurden die Feuchtigkeitsverhältnisse und der Grad der Eutrophierung bzw. die Luftqualität an den Bäumen der Gattungen Tilia und Acer anhand des bekannten Verhaltens der nachgewiesenen Flechten bezüglich Luftfeuchtigkeit/Niederschlägen, Eutrophierung und pH-Verhältnissen bewertet. 2017/2018 wurden 79 Flechtenarten und 14 lichenicole Pilzarten nachgewiesen. Die Gesamtzahl der im Untersuchungsgebiet nachgewiesenen epiphytischen Flechtenarten erhöhte sich damit auf 80. Die meisten Flechtenarten kommen an Tilia spec. (60), Acer pseudoplatanus (43) und A. platanoides (34) vor. Die häufigsten Arten sind Phaeophyscia orbicularis, Physcia adscendens, Ph. tenella und Xanthoria parietina. Candelariella xanthostigmoides, Flavoparmelia soredians, Hyperphyscia adglutinata, Intralichen lichenum, Lecanora subcarpinea, Parmelia serrana, Parmelina quercina und Taeniolella delicata sind Neufunde für Mecklenburg-Vorpommern. Hinsichtlich der Verbreitung und des Charakters der Fundorte können die nachgewiesenen Flechten und lichenicolen Pilze drei Gruppen zugeordnet werden: 1. Arten, die auch an stark befahrenen Straßen oder in eng bebauten Wohngebieten mit wenig Grünflächen und in den Stadtgebieten Kröpeliner Tor-Vorstadt oder Stadtmitte vorkamen, 2. Arten, die auch noch in locker bebauten, grünreichen Wohngebieten und in kleineren Grünlagen der Neubaugebiete vorkamen, aber nicht mehr in der Kröpeliner Tor-Vorstadt und Stadtmitte nachgewiesen wurden, 3. Arten, die nur an Bäumen vorkamen, die sich in der Nähe zum Offenland befinden. Der ersten Gruppe wurden 22, der zweiten Gruppe 32 und der dritten Gruppe 25 Flechtenarten zugeordnet. Dem Verhältnis zwischen der Frequenzsumme der stark nitrophytischen Arten und der Frequenzsumme der a-, schwach und mäßig nitrophytischen Arten auf den Bäumen entsprechend, wird die Luftqualität an 15 Bäumen der Gattungen Acer und Tilia für gut, an 14 Bäumen für mäßig und an 13 Bäumen für schlecht befunden. Auf der Grundlage des Verhältnisses zwischen der Anzahl basiphytischer Arten und der Summe an Arten an sauren oder/und subneutralen Borken wurden sechs Standorte als wenig, 21 Standorte als mäßig und 15 Standorte als stark schadstoffbelastet eingestuft. Meso- bis hygrophytische Flechten kamen an zehn Standorten mit nur ein oder zwei Arten, an 21 Standorten mit drei bis fünf Arten und an elf Standorten mit mehr als fünf Arten vor. Die epiphytische Flechtenflora hat sich in Rostock seit 1995 gravierend verändert. Es nahmen 69 Flechten im Bestand zu, von denen sich 52 Arten erst nach 1995 angesiedelt haben. Bei 32 Flechten ist eine schwache, bei 19 Flechten eine mäßige und bei 18 Flechten eine starke Zunahme zu verzeichnen. Lecanora conizaeoides kam 2017/2018 nicht mehr vor, vier Arten haben im Bestand abgenommen. Changes in the epiphytic lichen flora in the urban area of Rostock between 1994/1995 and 2017/2018 In 2017/2018, diversity and quantity of lichens were studied on 53 trees in Rostock city. The results were compared with the results of a mapping project in 1994/1995. Humidity conditions and degree of eutrophication at trees of the genera Tilia and Acer were interpreted with reference to the known indicator characteristcs of the lichen species concerning air humidity/precipitation, eutrophication and pH conditions. In 2017/2018, 79 lichen species and 14 lichenicolous fungus species were found. The total number of lichens increased to 80 species. Most species were found on Tilia spec. (60), Acer pseudoplatanus (43) and A. platanoides (34). The most common species are Phaeophyscia orbicularis, Physcia adscendens, Ph. tenella and Xanthoria parietina. Candelariella xanthostigmoides, Flavoparmelia soredians, Hyperphyscia adglutinata, Intralichen lichenum, Lecanora subcarpinea, Parmelia serrana, Parmelina quercina and Taeniolella delicata are new to Mecklenburg-Western Pomerania. The distribution and characteristics of the collection sites allow for deviding the species into three groups: 1. species also present along streets with heavy traffic or in densely populated residential areas with a lack of green areas and in the districts Kröpeliner Tor-Vorstadt or City Centre, 2. species still present in sparse residential areas and smaller green areas but not in the districts Kröpeliner Tor-Vorstadt or City Centre, 3. species only colonizing trees close to the open landscape. Twenty-two species were assigned to the first, 32 species to the second and 25 species to the latter group. According to the proportion between the sum of frequencies of the strongly nitrophytic lichens and the sum of frequencies of the anitrophytic and moderately nitrophytic lichens on trees, air quality was indicated to be good at 15 trees of the genera Acer and Tilia, moderate at 14 trees and bad at 13 trees. Based on the proportion between the number of basiphytic lichen species and the sum of species colonizing acidophytic and subneutrophytic bark, six locations were categorized as little, 21 locations as moderately and 15 locations as strongly polluted. Mesophytic or hygrophytic lichens were present with only one or two species at ten locations, three to five species at 21 locations and over five species at 11 locations. Within the period under consideration, the epiphytic lichen flora of the Rostock urban area changed considerably. The populations of 69 lichen species increased, with 52 lichens newly establishing after 1995. In 32 lichen species a slight, in 19 species a moderate, and in 18 species a strong population increase was recorded. Lecanora conizaeoides disappeared and the populations of four species decreased.


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