scholarly journals Effect of interaction between PPARG, PPARA and ADIPOQ gene variants and dietary fatty acids on plasma lipid profile and adiponectin concentration in a large intervention study

2011 ◽  
Vol 71 (1) ◽  
pp. 141-153 ◽  
Author(s):  
Aseel AlSaleh ◽  
Thomas A. B. Sanders ◽  
Sandra D. O'Dell
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Plasma Lipid ◽  
Serum Adiponectin ◽  
Gene Variants ◽  
Low Fat ◽  
Genotype Interaction ◽  
Mufa Diet

Unsaturated fatty acids are ligands of PPAR-γ, which up-regulates genes involved in fatty acid transport and TAG synthesis and the insulin-sensitising adipokine adiponectin, which activates fatty acid β-oxidation via PPAR-α action in liver. We investigated the effect of dietary fatty acid interaction with PPARG, PPARA and ADIPOQ gene variants on plasma lipid and adiponectin concentrations in the Reading Imperial Surrey Cambridge King's study, a five-centre, parallel design, randomised controlled trial of 466 subjects at increased cardiometabolic risk. After a 4-week run-in to baseline, SFA was replaced by MUFA or carbohydrate (low fat) in isoenergetic diets for 24 weeks. Habitual dietary PUFA:SFA ratio×PPARG Pro12Ala genotype interaction influenced plasma total cholesterol (P=0·02), LDL-cholesterol (P=0·002) and TAG (P=0·02) concentrations in White subjects. PPARA Val162Leu×PPARG Pro12Ala genotype interaction influenced total cholesterol (P=0·04) and TAG (P=0·03) concentrations at baseline. After high-MUFA and low-fat diets, total cholesterol and LDL-cholesterol were reduced (P<0·001) and gene×gene interaction determined LDL-cholesterol (P=0·003) and small dense LDL as a proportion of LDL (P=0·012). At baseline, ADIPOQ −10066 G/A A-allele was associated with lower serum adiponectin (n 360; P=0·03) in White subjects. After the high-MUFA diet, serum adiponectin increased in GG subjects and decreased in A-allele carriers (P=0·006 for difference). In GG, adiponectin increased with age after the high MUFA and decreased after the low-fat diet (P=0·003 for difference at 60 years). In conclusion, in Whites, high dietary PUFA:SFA would help to reduce plasma cholesterol and TAG in PPARG Ala12 carriers. In ADIPOQ −10066 GG homozygotes, a high-MUFA diet may help to increase adiponectin with advancing age.

scholarly journals Cholesterol reduction using manufactured foods high in monounsaturated fatty acids: a randomized crossover study

1999 ◽  
Vol 81 (6) ◽  
pp. 439-446 ◽  
Author(s):  
C. M. Williams ◽  
J. A. Francis-Knapper ◽  
D. Webb ◽  
C. A. Brookes ◽  
A. Zampelas ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Ldl Cholesterol ◽  
Control Diet ◽  
Saturated Fatty Acids ◽  
Saturated Fat ◽  
Young Men ◽  
Cholesterol Concentration ◽  
Middle Aged ◽  
Mufa Diet

In two separate studies, the cholesterol-lowering efficacy of a diet high in monounsaturated fatty acids (MUFA) was evaluated by means of a randomized crossover trial. In both studies subjects were randomized to receive either a high-MUFA diet or the control diet first, which they followed for a period of 8 weeks; following a washout period of 4–6 weeks they were transferred onto the opposing diet for a further period of 8 weeks. In one study subjects were healthy middle-aged men (n30), and in the other they were young men (n23) with a family history of CHD recruited from two centres (Guildford and Dublin). The two studies were conducted over the same time period using identical foods and study designs. Subjects consumed 38 % energy as fat, with 18 % energy as MUFA and 10 % as saturated fatty acids (MUFA diet), or 13 % energy as MUFA and 16 % as saturated fatty acids (control diet). The polyunsaturated fatty acid content of each diet was 7 %. The diets were achieved by providing subjects with manufactured foods such as spreads, ‘ready meals’, biscuits, puddings and breads, which, apart from their fatty acid compositions, were identical for both diets. Subjects were blind to which of the diets they were following on both arms of the study. Weight changes on the diets were less than 1 kg. In the groups combined (n53) mean total and LDL-cholesterol levels were significantly lower at the end of the MUFA diet than the control diet by 0·29 (sd 0·61) mmol/l (P< 0·001) and 0·38 (sd 0·64) mmol/l (P< 0·0001) respectively. In middle-aged men these differences were due to a mean reduction in LDL-cholesterol of – 11 (sd 12) % on the MUFA diet with no change on the control diet (−1·1 (sd 10) %). In young men the differences were due to an increase in LDL-cholesterol concentration on the control diet of +6·2 (sd 13) % and a decrease on the MUFA diet of −7·8 (sd 20) %. Differences in the responses of middle-aged and young men to the two diets did not appear to be due to differences in their habitual baseline diets which were generally similar, but appeared to reflect the lower baseline cholesterol concentrations in the younger men. There was a moderately strong and statistically significant inverse correlation between the change in LDL-cholesterol concentration on each diet and the baseline fasting LDL-cholesterol concentration (r– 0·49;P< 0·0005). In conclusion, diets in which saturated fat is partially replaced by MUFA can achieve significant reductions in total and LDL-cholesterol concentrations, even when total fat and energy intakes are maintained. The dietary approach used to alter fatty acid intakes would be appropriate for achieving reductions in saturated fat intakes in whole populations.

scholarly journals Effects of reduced-fat diets with different fatty acid compositions on serum lipoprotein lipids and apolipoproteins

1998 ◽  
Vol 1 (2) ◽  
pp. 109-116 ◽  
Author(s):  
Antti Aro ◽  
Pirjo Pietinen ◽  
Liisa M Valsta ◽  
Anu M Turpeinen ◽  
Christian Ehnholm ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Ldl Cholesterol ◽  
Saturated Fatty Acids ◽  
Serum Lipoprotein ◽  
Low Fat ◽  
Serum Triglycerides ◽  
Habitual Diet ◽  
Total Fat ◽  
Fat Diets

AbstractObjective:To compare the effects on serum lipoproteins of three isocaloric diets with reduced total fat and saturated fatty acid (SFA) contents but with different proportions of monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA).Design:A low-fat diet (LF) provided 20 en% fat, 7.9% SFA, 7.8% MUFA, 3.0% PUFA; a high-PUFA diet (HP) 26 en% fat, 7.5% SFA, 8.2% MUFA, 8.1% PUFA; and a high-MUFA diet (HM) 26 en% fat, 7.3% SFA, 14.1% MUFA, 3.2% PUFA. Diets were consumed for 8 weeks in a parallel design, after 2 weeks on a habitual diet with 33–34 en% fat, 13–14% SFA, 12% MUFA, 6% PUFA, and followed by an 8-week period on habitual diet. Compliance to diet was monitored by repeated food records and weekly visits to a nutritionist.Subjects:45 free-living, middle-aged couples who were randomly allocated into the three diet groups; 43 men and 44 women completed the study.Results:During the diet periods, a small but significant reduction in body weight of 0.4–1.0kg was observed in all groups. Similar and significantreductions of mean weight-adjusted serum total cholesterol (4–8%), low-density lipoprotein (LDL) cholesterol (7–11%), and high-densitylipoprotein (HDL) cholesterol (8–11%) were observed during the diets. HDL2-cholesterol and apoprotein (apo) A-I levels were reduced whereas HDL3-cholesterol and apoA-II increased. ApoB was significantly decreased during the HM diet only. Serum triglycerides increased significantly during diets LF (25%, P< 0.01) and HP (19%, P< 0.05) but not during diet HM (5%, NS).Conclusions:Reduction in the intake of total fat and saturated fatty acids reduced serum LDL-cholesterol and HDL2-cholesterol concentrations irrespective of the relative proportions of MUFA and PUFA in the diets. The results suggest that there might be some advantage in increasing the proportion of MUFA in low-fat diets, since the HM diet rich in MUFA reduced apoB and slightly attenuated the increase in serum triglycerides that is commonly associated with dietary fat reduction.

scholarly journals Effects of yoghurt enriched with plant sterols on serum lipids in patients with moderate hypercholesterolaemia

2001 ◽  
Vol 86 (2) ◽  
pp. 233-239 ◽  
Author(s):  
Robert Volpe ◽  
Leena Niittynen ◽  
Riitta Korpela ◽  
Cesare Sirtori ◽  
Antonello Bucci ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Serum Lipids ◽  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Plant Sterols ◽  
Dependent Manner ◽  
Low Fat ◽  
Treatment Groups ◽  
Cholesterol Levels ◽  
Dose Dependent

The objective of the present study was to assess the effect of consumption of a yoghurt-based drink enriched with 1–2 g plant sterols/d on serum lipids, transaminases, vitamins and hormone status in patients with primary moderate hypercholesterolaemia. Thirty patients were randomly assigned to one of two treatment groups: a low-fat low-lactose yoghurt-based drink enriched with 1 g plant sterol extracted from soyabean/dv.a low-fat low-lactose yoghurt, for a period of 4 weeks. After a 2-week wash-out period, patients were crossed over for an additional 4-week period. Second, after a 4-week wash-out period, eleven patients were treated with 2 g plant sterols/d in a second open part of the study for a period of 8 weeks. The yoghurt enriched with plant sterols significantly reduced, in a dose-dependent manner, serum total cholesterol and LDL-cholesterol levels and LDL-cholesterol:HDL-cholesterol (P<0·001), whereas no changes were observed in HDL-cholesterol and triacylglycerol levels, either in the first or the second part of the study. There were only slight, not statistically significant, differences in serum transaminase, vitamin and hormone levels. To conclude, a low-fat yoghurt-based drink moderately enriched with plant sterols may lower total cholesterol and LDL-cholesterol effectively in patients with primary moderate hypercholesterolaemia.

scholarly journals Plasma fatty acid profile in dairy cows associated with the inclusion of annatto in their diet

2020 ◽  
Vol 42 ◽  
pp. e47651
Author(s):  
Clarissa Sampaio de Oliveira Lima ◽  
Albericio Pereira de Andrade ◽  
André Luiz Rodrigues Magalhães ◽  
Omer Cavalcanti de Almeida ◽  
Sebastião Inocêncio Guido ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Fatty Acid Profile ◽  
Plasma Lipid ◽  
High Density Lipoproteins ◽  
Plasma Fatty Acid ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Plasma Fatty Acid Profile

The objective of this study was to evaluate the plasma lipid profile and plasma fatty acids of dairy cows receiving diets supplemented with annatto. A total of 32 Holstein cows (550 kg), distributed in a completely randomized design, were allocated to individual stalls and submitted to following treatments: C0 = no annatto; C4 = inclusion of annatto at 4 g kg-1 dry matter (DM) of diet (0.07 g bixin kg-1 diet); C5 = inclusion of annatto at 5 g kg-1 DM of diet (0.09 g bixin kg-1 diet); and C7 = inclusion of annatto at 7 g kg-1 DM of diet (0.12 g bixin kg-1 diet). Blood samples were collected via epigastric vein puncture, centrifuged, and frozen for subsequent analysis. The results indicate that the inclusion (p > 0.05) of annatto does not decrease the total cholesterol or low and high density lipoproteins. However, it impacts the profile of fatty acids, evidenced by the reduction (p < 0.05) in levels of hypercholesterolemic fatty acids viz, myristic acid and palmitic acid.  It also causes an increase in the levels of arachidonic acid, rumenic acid, linoleic acid, and total polyunsaturated fatty acids. Therefore, bixin included in the diets of dairy cows induces changes in the plasma fatty acid profile.

scholarly journals Plasma lipid modifications in elderly people after administration of two virgin olive oils of the same variety (Olea europaeavar.hojiblanca) with different triacylglycerol composition

2003 ◽  
Vol 89 (6) ◽  
pp. 819-826 ◽  
Author(s):  
Javier S. Perona ◽  
Julio Cañizares ◽  
Emilio Montero ◽  
José M. Sánchez-Domínguez ◽  
Valentina Ruiz-Gutierrez
Keyword(s):  
Blood Pressure ◽  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Plasma Lipid ◽  
Cholesteryl Esters ◽  
Minor Components ◽  
Plasma Total Cholesterol ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Triacylglycerol Molecular Species

In the present study we examined whether two virgin olive oils (VOO1 and VOO2), of the same variety (Olea europaeavar.hojiblanca) and with a similar composition of minor components but differing in the content of triacylglycerol molecular species, had different effects on blood pressure and plasma lipid levels in a healthy elderly population. Thirty-one participants, aged 84·9 (SD 6·4) years, were asked to participate in the study. No differences were found with regard to blood pressure after both experimental periods (VOO1 and VOO2). However, plasma total cholesterol and LDL-cholesterol were reduced only after VOO1 (P<0·01). The reduction of plasma cholesterol concentrations was related to the incorporation of oleic acid into plasma cholesteryl esters and phospholipids, which was higher after VOO1 (P<0·01). Indeed, the oleic acid concentration in cholesteryl esters and phospholipids strongly correlated with plasma total cholesterol and LDL-cholesterol levels in all experimental periods studied (r2>0·418,P<0·07), except for phospholipids in VOO1 (P=0·130 for total cholesterol andP=0·360 for LDL-cholesterol). These results have demonstrated that blood pressure and plasma lipids can be modified by the consumption of VOO in elderly people, but that the extent of such modification depends on the composition and amount of active minor components and triacylglycerol molecular species.

scholarly journals The lipoprotein/lipid profile is modulated by a gene–diet interaction effect between polymorphisms in the liver X receptor-α and dietary cholesterol intake in French-Canadians

2007 ◽  
Vol 97 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Julie Robitaille ◽  
Alain Houde ◽  
Simone Lemieux ◽  
Daniel Gaudet ◽  
Louis Pérusse ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Dietary Cholesterol ◽  
Plasma Lipid ◽  
Plasma Lipid Profile ◽  
Plasma Total Cholesterol ◽  
Lipoprotein Lipid ◽  
Liver X Receptor Α ◽  
Cholesterol Intake

Genetic and nutritional factors interact together and modulate the plasma lipid profile. We identified variations in the gene encoding the liver X receptor α (LXRα) and investigated their effects on the plasma lipoprotein/lipid profile. We also examined whether the association between cholesterol intake and plasma lipid profile was modulated by LXRα variants. The LXRα gene was sequenced in thirty-five French-Canadian men with high plasma total cholesterol (>5·0 mmol/l) and LDL-cholesterol (>3·5 mmol/l) concentrations. Dietary cholesterol was obtained from a food-frequency questionnaire. The LXRα c.-115G>A, c.-840C>A and c.-1830T>C genotypes were determined by direct sequencing in 732 subjects. Molecular screening of the LXRα gene revealed sixteen variants. Genotypes c.-115G>A, c.-840C>A and c.-1830T>C (rare allele frequency of 14·3 %, 14·2 % and 11·0 %, respectively) were analysed further. Plasma total cholesterol concentrations were higher in carriers of the -115A, -840A and -1830C allele, compared with the -115G/G, -840C/C and -1830T/T homozygotes (P ≤ 0·05). In a model including the c.-115G>A polymorphism, cholesterol intake, the interaction term c.-115G>A × cholesterol intake (mg/d) and covariates, LXRα-115G>A explained 1·8 % and 2·1 % of the variance in total cholesterol and LDL-cholesterol concentrations (P = 0·02 andP = 0·01), whereas the interaction term explained 2·9 % (P = 0·002) and 2·8 % (P = 0·005), respectively. When subjects were divided into four groups according to the median of cholesterol (290·8 mg) and -115G>A genotypes, high cholesterol intake was associated with higher cholesterol levels in -115A carriers. Similar results were observed for c.-840C>A and c.-1830T>C. These results suggest that cholesterol intake interacts with LXRα variants to modulate the plasma lipid profile.

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