Plasma riboflavin concentration as novel indicator for vitamin-B2 status assessment: suggested cutoffs and its association with vitamin-B6 status in women

AbstractRiboflavin (vitamin B2), as the coenzymes flavin mononucleotide (FMN) and flavin dinucleotide (FAD), is essential for oxidation-reduction reactions and energy metabolism. Riboflavin also interacts with vitamin B12, B6 and folate in one-carbon metabolism, and is required for the conversion of dietary vitamin B6 forms to the coenzyme pyridoxal 5’-phosphate (PLP). Biochemical riboflavin status is rarely measured given the lack of convenient and accessible biomarkers. The current gold-standard marker is erythrocyte glutathione reductase activation coefficient (EGRac) that involves laborious sample processing. High prevalence of riboflavin deficiency (EGRac ≥ 1.4) and suboptimal status (EGRac of 1.3–1.39) have been reported in the UK and Ireland; yet the functional significance is unclear. Plasma riboflavin concentration may serve as an alternative indicator; its association with related metabolites has not yet been investigated. Secondary analysis was conducted to determine the change-point of plasma riboflavin with EGRac, to derive a reference interval for plasma riboflavin, and to determine the association of riboflavin status with plasma PLP, using data of 223 older adult women from a cross-sectional study. Fasting blood samples and sociodemographic, anthropometric and dietary data were available for a convenience sample of 223 older adult women. Plasma PLP and related metabolites were quantified using isotope-dilution liquid chromatography-tandem mass spectrometry. The change-point (95% CI) between EGRac and plasma riboflavin occurred at plasma riboflavin concentration of 26.5 (20.5; 32.5) nmol/L (with EGRac of 1.25). The median (IQR) plasma riboflavin concentration was 15.7 (11.2, 23.8); and the upper and lower limits (90%CI) of the central 95% reference interval were 6.70 (6.33, 7.79) and 64.2 (55.0, 74.6) nmol/L, respectively. Plasma PLP (geometric mean (95%CI)) was significantly lower in women with riboflavin deficiency, 54.0 (46.8, 62.2) nmol/L (n = 64), and suboptimal riboflavin status, 56.1 (48.9, 64.3) nmol/L (n = 48), compared to those with riboflavin adequacy, 135 (112, 161) nmol/L (n = 110). Plasma PLP was positively associated with plasma riboflavin concentration after adjustment for total B6 intake, age, ethnicity, BMI, education, household income and C-reactive protein concentration [β (95% CI) = 1.92 (.670, 3.17) nmol/L; p = 0.003]; a significant interaction between plasma riboflavin and total dietary B6 intake was observed (p = 0.024). In conclusion, we are presenting for the first time a reference range for plasma riboflavin concentration and its change-point with EGRac in healthy women. Vitamin B6 status is strongly associated with riboflavin status; more research is needed to elucidate this relationship in a larger sample and ideally intervention study.

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Associations of dietary vitamin B1, vitamin B2, vitamin B6, and vitamin B12 with the risk of depression: a systematic review and meta-analysis

Nutrition Reviews ◽

10.1093/nutrit/nuab014 ◽

2021 ◽

Author(s):

Yanjun Wu ◽

Liming Zhang ◽

Suyun Li ◽

Dongfeng Zhang

Keyword(s):

Systematic Review ◽

Vitamin B12 ◽

Vitamin B6 ◽

Fixed Effects ◽

Data Extraction ◽

Meta Analysis ◽

Vitamin B1 ◽

Dietary Vitamin ◽

Vitamin B2 ◽

Fixed Effects Model

Abstract Context The results from epidemiologic studies on the intake of dietary vitamin B1, B2, B6, and B12 and association with risk of developing depression have been inconsistent. Objective The purpose of this systematic review and meta-analysis was to summarize the existing evidence and synthesize the results. Data Sources The databases of Web of Science and PubMed were searched for relevant articles published in English until September 2020. Study Selection Observational studies that evaluated the associations between depression and dietary vitamin B1, B2, B6, and B12 were included in this study. Data Extraction The job of data extraction was undertaken by 2 authors, and the pooled relative risks (RRs) and 95% confidence intervals (CIs) were calculated using a fixed-effects model. Results Thirteen articles related to 18 studies were eligible for inclusion in this systematic review and meta-analysis. The pooled RR (95% CI) of depression for the highest vs the lowest category of dietary vitamin B1, B2, B6, and B12 was 0.69 (0.55–0.87), 0.77 (0.67–0.89), 0.81 (0.71–0.93), and 0.86 (0.75–0.99), respectively. The pooled RR (95% CI) of depression for the highest vs the lowest category of dietary vitamin B2 was 0.80 (0.64–0.99) in females and 0.83 (0.67–1.02) in males, for dietary vitamin B6 was 0.71 (0.59–0.86) in females and 0.92 (0.76–1.12) in males, and for dietary vitamin B12 was 0.79 (0.65–0.97) in females and 0.94 (0.77–1.15) in males. Conclusion This study suggested that the intake of dietary vitamin B1, B2, B6, and B12 may be inversely associated with the risk of depression; the inverse associations observed between depression and intake of dietary vitamin B2, B6, and B12 were significant in females, but not in males. Further studies are needed to confirm these results.

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Associations of dietary vitamin B1, vitamin B2, niacin, vitamin B6, vitamin B12 and folate equivalent intakes with metabolic syndrome

International Journal of Food Sciences and Nutrition ◽

10.1080/09637486.2020.1719390 ◽

2020 ◽

Vol 71 (6) ◽

pp. 738-749 ◽

Cited By ~ 2

Author(s):

Yanjun Wu ◽

Suyun Li ◽

Weijing Wang ◽

Dongfeng Zhang

Keyword(s):

Metabolic Syndrome ◽

Vitamin B12 ◽

Vitamin B6 ◽

Vitamin B1 ◽

Dietary Vitamin ◽

Vitamin B2

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Associations of dietary B vitamins intakes with depression in adults

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000720 ◽

2021 ◽

Author(s):

Yanjun Wu ◽

Suyun Li ◽

Weijing Wang ◽

Dongfeng Zhang

Keyword(s):

Vitamin B12 ◽

Vitamin B6 ◽

Vitamin B1 ◽

B Vitamins ◽

Dietary Vitamin ◽

Dietary Intakes ◽

Vitamin B2 ◽

Logistic Regression Models ◽

The People ◽

The Impact

Abstract. Background: The impact of the dietary B vitamins intakes on the development of depression has been scarcely investigated. Thus, this study aimed to examine the associations of dietary B vitamins intakes with the risk of depression in American adults. Methods: The data we used in this study were from the National Health and Nutrition Examination Survey (NHANES) 2007-2014. We used the Logistic regression models to analyze the associations of the dietary intakes of B vitamins with the risk of depression. Results: 17,732 individuals (8,623 males and 9,109 females) were enrolled in the study and they were all 18 or older. Compared to the lowest quartile of dietary intake, the ORs (95%CIs) of the highest quartile were 0.64 (0.50–0.82), 0.78 (0.62–0.97), 0.60 (0.47–0.78), 0.65 (0.50–0.84), and 0.71 (0.54–0.95) for vitamin B1, vitamin B2, niacin, vitamin B6, and vitamin B12, respectively. Compared to the people whose dietary intakes below the RDA in the model 2, those with intake meeting the RDA of vitamin B1 (OR: 0.68; 95%CI: 0.56–0.84), niacin (OR: 0.65; 95%CI: 0.51–0.81), B6 (OR: 0.65; 95%CI: 0.52–0.81), or B12 (OR: 0.65; 95%CI: 0.48–0.88) had a lower risk of depression, severally. We also found a nonlinear negative association between dietary vitamin B1, vitamin B2, niacin, vitamin B6, and vitamin B12 intakes and the risk of depression in the dose-response analyses, severally. Conclusions: Our results suggested that dietary vitamin B1, vitamin B2, niacin, vitamin B6, and vitamin B12 intakes may be inversely associated with the risk of depression.

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Dietary Riboflavin Intake and Riboflavin Status in Young Adult Women Living in Metro Vancouver, Canada

Current Developments in Nutrition ◽

10.1093/cdn/nzab021 ◽

2021 ◽

Vol 5 (4) ◽

Author(s):

Abeer M Aljaadi ◽

Alejandra M Wiedeman ◽

Susan I Barr ◽

Angela M Devlin ◽

Tim J Green

Keyword(s):

Young Adult ◽

Dairy Products ◽

Food Sources ◽

Adult Women ◽

Young Adult Women ◽

Riboflavin Deficiency ◽

The Past ◽

Metro Vancouver ◽

Chinese Ethnicity ◽

Riboflavin Status

ABSTRACT Background Nutrition surveys suggest that <10% of Canadian adults have inadequate riboflavin intakes. However, biochemical riboflavin deficiency [erythrocyte glutathione reductase activity coefficient (EGRac) ≥1.40] has been reported in 41% of young adult women living in Metro Vancouver. Canadian Chinese ethnicity comprise >25% of Vancouver's population and are postulated to have poorer riboflavin status than those of European ethnicity because they could be less likely to consume dairy products and fortified wheat. Objectives The objectives of this study were to determine dietary riboflavin intake and food sources, and to assess the association between riboflavin intake and status in young women of European (n = 107) and Chinese (n = 91) ethnicities living in Metro Vancouver, Canada. Methods This was a cross-sectional study conducted in women (aged 19–45 y). Women were healthy, not pregnant or breastfeeding, of European or Chinese ethnicities, and not taking riboflavin-containing supplements for the past 4 mo. Dietary riboflavin intake was assessed using the past-year Diet History Questionnaire II, and riboflavin status (EGRac) was measured in fasting venous blood samples. Results Only 7% of participants had dietary riboflavin intakes below the Estimated Average Requirement (0.9 mg/d), but 40% of women had biochemical riboflavin deficiency (EGRac ≥1.40). Although more Canadian women of European ethnicity than Chinese ethnicity had biochemical riboflavin deficiency (46% and 34%; P < 0.001), median dietary riboflavin intake did not differ (1.73 and 1.82 mg/d; P = 0.587). Dairy products and vegetables contributed the most to riboflavin intake. Energy-adjusted dietary riboflavin intake was inversely associated with EGRac (B = −0.04, 95% CI: −0.07, −0.01). However, after further adjustment the relation was not significant. Conclusions Overall, women of reproductive age living in Metro Vancouver, Canada, had a low prevalence of inadequate dietary riboflavin intake despite the high prevalence of apparent biochemical riboflavin deficiency.

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Symptom Change in Depression After CBT in Older Adult Women

PsycEXTRA Dataset ◽

10.1037/e608852013-001 ◽

2013 ◽

Author(s):

Ann C. Bilbrey ◽

Aleksandra Stepanenko ◽

Johanna Rengifo Nevarez ◽

Renee Marquett ◽

Dolores Gallagher-Thompson

Keyword(s):

Older Adult ◽

Adult Women ◽

Symptom Change

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A Social Media Game to Increase Physical Activity Among Older Adult Women

Case Medical Research ◽

10.31525/ct1-nct04095923 ◽

2019 ◽

Author(s):

Keyword(s):

Physical Activity ◽

Social Media ◽

Older Adult ◽

Adult Women

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Depression and Vegetarians: Association between Dietary Vitamin B6, B12 and Folate Intake and Global and Subcortical Brain Volumes

Nutrients ◽

10.3390/nu13061790 ◽

2021 ◽

Vol 13 (6) ◽

pp. 1790

Author(s):

Samuel Berkins ◽

Helgi Birgir Schiöth ◽

Gull Rukh

Keyword(s):

Vitamin B12 ◽

Brain Structure ◽

Vitamin B6 ◽

Significant Interaction ◽

Longitudinal Design ◽

Folate Intake ◽

Dietary Vitamin ◽

Brain Volumes ◽

Subcortical Brain ◽

Depression Status

Deficiency of vitamin B6 and vitamin B12, mostly in vegetarians, is found to be associated with depression and adverse neurological function. We investigated whether vitamin B6, B12, and folate have an effect on brain structure, especially among depressed people who follow a specific diet. The study sample comprised 9426 participants from the UK Biobank cohort with a mean age of 62.4 years. A generalized linear model controlling for age, sex, body mass index, ethnicity, town send deprivation index, educational qualification, smoking, and alcohol intake was used to test the association between study groups and structural brain volumes. Depression was more prevalent, and intake of vitamin B6 and B12 was lower among vegetarians, while non-vegetarians had a lower intake of folate. Overall, no significant association was observed between vitamin B6, B12, and folate intakes and both global and subcortical brain volumes among participants with depression. However, vitamin B12 intake was positively associated with right pallidum among non-depressed participants, and a significant interaction between vitamin B12 intake and depression status on the right pallidum was observed. Also, a significant interaction between folate intake and depression status on grey matter (GM) volume and left thalamus was observed. Upon diet stratification, folate intake is associated with total brain volume and GM volume among vegetarians with depression. Furthermore, no significant associations were observed for subcortical regions. Our findings suggest that dietary intake of vitamin B6 and B12 might have an effect on brain structure. Vegetarians, particularly those who suffer from depression may benefit from supplementing their diets with vitamins B6, B12, and folate to ensure brain health. Further studies, especially with a larger sample size and longitudinal design, are needed to confirm these findings.

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Probiotic Potential of a Novel Vitamin B2-Overproducing Lactobacillus plantarum Strain, HY7715, Isolated from Kimchi

Applied Sciences ◽

10.3390/app11135765 ◽

2021 ◽

Vol 11 (13) ◽

pp. 5765

Author(s):

Joo-Yun Kim ◽

Eun-Jung Choi ◽

Jae-Ho Lee ◽

Myeong-Seok Yoo ◽

Keon Heo ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Plantarum ◽

Probiotic Strain ◽

Optimal Growth ◽

Control Group ◽

High Survival ◽

Vitamin B2 ◽

Riboflavin Deficiency

Vitamin B2, also known as riboflavin, is essential for maintaining human health. The purpose of this study was to isolate novel lactic acid bacteria that overproduce vitamin B2 and to validate their potential as probiotics. In this study, Lactobacillus plantarum HY7715 (HY7715) was selected among lactic acid bacteria isolated from Kimchi. HY7715 showed a very high riboflavin-producing ability compared to the control strain due to the high expression of ribA, ribB, ribC, ribH, and ribG genes. HY7715 produced 34.5 ± 2.41 mg/L of riboflavin for 24 h without consuming riboflavin in the medium under optimal growth conditions. It was able to produce riboflavin in an in vitro model of the intestinal environment. In addition, when riboflavin deficiency was induced in mice through nutritional restriction, higher levels of riboflavin were detected in plasma and urine in the HY7715 administration group than in the control group. HY7715 showed high survival rate in simulated gastrointestinal conditions and had antibiotic resistance below the cutoff MIC value suggested by the European Food Safety Authority; moreover, it did not cause hemolysis. In conclusion, HY7715 could be considered a beneficial probiotic strain for human and animal applications, suggesting that it could be a new alternative to address riboflavin deficiency.

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Binge Eating Among Older Women: Prevalence Rates and Health Correlates Across Three Independent Samples

10.21203/rs.3.rs-726279/v1 ◽

2021 ◽

Author(s):

Salome Adelia Wilfred ◽

Carolyn Black Becker ◽

Kathryn E Kanzler ◽

Nicolas Musi ◽

Sara E Espinoza ◽

...

Keyword(s):

Binge Eating ◽

Older Women ◽

Older Adult ◽

Negative Mood ◽

Doctoral Degree ◽

Adult Women ◽

Prevalence Rates ◽

Food Security Status ◽

Three Samples ◽

Original Goal

Abstract BackgroundEmerging research indicates that older women struggle with binge eating (BE; consuming unusually large amounts of food in one siting while feeling a loss of control) more frequently than once thought. Yet, health correlates of BE in older adult populations are poorly understood. The original goal of the study was to investigate BE prevalence, frequency, and health correlates in a sample of older adult women. Following surprising findings in this first study, we then aimed to replicate findings in two additional samples of older adult women. MethodUsing self-reported frequencies of BE from three separate samples of older women with very different demographics, we compared BE prevalence, frequency, and health correlates among older women. Study 1 (N = 185) includes data collected online (86% White; 59% overweight/obese status). Study 2 (N = 64) was conducted in person at a local food pantry (65% Hispanic; 47% household income < $10,000/year). Study 3 (N = 100) comprises data collected online (72% White; 50% Masters/Doctoral Degree). ResultsPer DSM-5 frequency criterion of BE at least weekly, we found prevalence rates ranging from 19%-26% across the three samples. Correlates of BE frequency included elevated negative mood, worry, BMI, and less nutritious food consumption. ConclusionsAcross three very different samples in terms of race/ethnicity, education, food security status, measurements, and sampling methodology, we found fairly consistent rates of self-reported BE at least weekly (19-26%). BE is related to negative outcomes among older women, supporting the need for more research in this population.

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