Electron-microscopical observations on the body wall of the third-stage larva of Haemonchus placei

Parasitology ◽  
1970 ◽  
Vol 60 (3) ◽  
pp. 411-416 ◽  
Author(s):  
Kenneth Smith
Keyword(s):  
Body Wall ◽  
Muscle Cells ◽  
Cortical Layer ◽  
The Body ◽  
Stage Larva ◽  
Dense Layer ◽  
The Third ◽  
Third Stage ◽  
Electron Microscopical ◽  
Inner Cortex

SUMMARYThe ultrastructure of the body wall of the third-stage larva of Haemonckus placei was studied. The cuticle was found to consist of eight layers: a thin outer layer, a membrane-bounded layer, an electron-dense layer, a thin irregular layer, an inner cortical layer, a matrix layer, a striated layer and a fibril layer. Interposed between the inner cortex and matrix were two transverse fibres.The region between the fibril layers and the contractile part of the muscle cells was occupied by the hypodermis, which enlarged to form the dorsal, ventral and lateral cords. Within the cords lay hypodermal cells, nerves, crystalline inclusions and an excretory canal.The sarcoplasmic part of the muscle cells was rich in glycogen and contained numerous mitochondria. Myofibrils of two types were present in the contractile part of the cell.I am grateful to Dr D. W. Brocklesby for his help and advice and to Mr E. Harness for the production and supply of third-stage larvae. I would also like to thank Dr D. L. Lee and Mr W. G. MacMillan for helpful discussions.

The fine structure of the body-wall and the growth of the cuticle in the adult nematode Ascaris lumbricoides

1965 ◽  
Vol s3-106 (73) ◽  
pp. 83-91
Author(s):  
B. D. WATSON
Keyword(s):  
Fine Structure ◽  
Ascaris Lumbricoides ◽  
Body Wall ◽  
Muscle Cells ◽  
Particulate Material ◽  
Cortical Layer ◽  
The Body ◽  
Homogeneous Layer ◽  
Matrix Layer ◽  
The Matrix

The fine structure of the body-wall in Ascaris lumbricoides was investigated by electron-microscopical techniques. The body-wall is composed of a cuticle, epidermis, and a single layer of muscle cells. The cuticle contains several layers: a superficial membrane, a cortex, the ‘fibrillar layer’, a matrix or homogeneous layer, 3 fibre layers, and a basal lamella. The cortex is divided into a homogeneous, external cortical layer, and a fibrous, internal cortical layer. The ‘fibrillar layer’ is a series of canals which extend from the inner part of the matrix layer to the cortex. The canals have distinct walls but no contents were demonstrated. No fibres or lamellae could, be detected in the matrix layer. The strands of the fibre layers and the basal lamella are formed from fine fibrils, less than 10 mµ in diameter. There is an inner system of canals that links the epidermis with the basal lamella and the fibre layers. The epidermis has a network of fibres, some of which attach the muscle cells to the cuticle. The muscle cell contains myofilaments of 2 types, an array of large filaments about 30 mµ. in diameter, each surrounded by a number of smaller filaments about 5 to 7 mµ in diameter. Glycogen occurs in the epidermis and muscle and is identified as granules; there tends to be a clumping together of granules to form deposits about 100 mµ in diameter. External to the sarcolemma lies a connective tissue sheath which contains collagen, probably in the form of fibrillar and particulate material. The cuticles of young adult Ascaris have a basic structure similar to that of the fully grown worms. During the growth of the adult worm the cuticle increases in volume, and this increase involves all the layers of the cuticle. The homogeneous or matrix layer increases in thickness more rapidly than the fibre layers, and both of these layers grow faster than the cortex. Ribonucleic acid is more abundant in the epidermis of young adults than in the fully grown worms and this is correlated with the development of endoplasmic reticulum and ribosomes. It is suggested that the extensive canal system in the cuticle transports materials to all layers of the cuticle.

The Nasal Bot Fly, Cephenomyia auribarbis Meigen (Diptera, Tachinidae) of the Red Deer, Cervus elaphus L

Parasitology ◽  
1932 ◽  
Vol 24 (2) ◽  
pp. 185-195 ◽  
Author(s):  
A. E. Cameron
Keyword(s):  
Peritoneal Cavity ◽  
Cervus Elaphus ◽  
Red Deer ◽  
Body Wall ◽  
The Body ◽  
Stage Larva ◽  
Pelvic Region ◽  
Third Stage ◽  
Pharyngeal Apparatus ◽  
Gastric Mucous

1. Cephenomyia auribarbis Mg., a tachino-oestrid parasite of the red deer (Cervus elaphus L.), is distributed throughout the deer forests of Scotland. The adult is found on the wing during June and July.2. The larva normally occurs attached to the walls of the nasal passages and pharynx, where it remains for 10 or 11 months. At maturity it drops to the ground and pupates beneath the surface. The adult emerges in 3–4 weeks.3. In January, 1931, a larva of the early third instar was recovered from a stag shot at Blair Atholl, Perthshire. It was found in fat in the pelvic region and not in the nostril or pharynx as might have been expected.4. It is suggested that the larva may have been swallowed by the host and bored its way through the wall of the gut into the peritoneal cavity, whence it had wandered to the pelvic region; or else that a first-stage larva had been accidentally deposited by a female on the hide and had bored through the body wall to the peritoneal cavity.5. The adult female and the early third-stage larva are described and illustrated.6. A comparison is made between the cephalo-pharyngeal apparatus of Cephenomyia auribarbis, Cephalomyia ovis, and Gastrophilus intestinalis. It is noted that the basilar sclerite of the first two species is provided with an accessory lateral appendage on each side. A greater surface is thus furnished for the attachment of the large depressor muscles of the buccal hooks. The larva thus securely holds its ground in the nasal cavities and pharynx, where the risks of summary ejection are always present. In G. intestinalis the accessory appendage is absent. The chances of the larva of this species losing its hold on the gastric mucous membrane of the host are very slight.

An electron microscope study of the body wall of the third-stage larva of Nippostrongylus brasiliensis

Parasitology ◽  
1966 ◽  
Vol 56 (1) ◽  
pp. 127-135 ◽  
Author(s):  
D. L. Lee
Keyword(s):  
Plasma Membrane ◽  
Electron Microscope ◽  
Basement Membrane ◽  
Stage Larva ◽  
Nippostrongylus Brasiliensis ◽  
The Third ◽  
Third Stage ◽  
Matrix Layer ◽  
Inner Cortex ◽  
Excretory Canal

The cuticle of the third-stage larva of Nippostrongylus brasiliensis consists of seven layers: an outer triple-layered membrane, a double-layered outer cortex, an inner cortex, a matrix layer, a striated layer and two fibril layers. In each ‘annule’ two fibres run transversely around the nematode and lie between the inner cortex and the matrix layer. There is no basement lamella.The hypodermis is a thin layer between the muscles and the cuticle, but expands to form the dorsal, ventral and lateral cords. The nerves lie between the plasma membrane of the hypodermis and the basement membrane or between the plasma membrane of the hypodermis and the sarcolemma of the muscles. The muscle cells are typical of those described previously for nematodes. The ‘myofibrils’ are apparently similar to those of Ascaris.An excretory canal is present in each lateral cord and is enclosed by the basement membrane but is not embedded in the hypodermal tissue. Numerous small vesicles appear to move across the wall of the excretory canal and open into the central lumen.I am grateful to Dr P. Tate, Dr R. W. Horne and Dr K. A. Wright for helpful discussions, to Professor C. P. Read and Dr A. Enders for the use of facilities at Rice University, Houston, Texas and to Professor J. D. Boyd for permission to use the electron microscope in the Department of Anatomy. Thanks are also due to Mr A. J. Page for technical assistance.

The cuticle of adultNippostrongylus brasiliensis

Parasitology ◽  
1965 ◽  
Vol 55 (1) ◽  
pp. 173-181 ◽  
Author(s):  
D. L. Lee
Keyword(s):  
Electron Microscope ◽  
Body Wall ◽  
Technical Assistance ◽  
Cortical Layer ◽  
The Body ◽  
Collagen Fibrils ◽  
Intestinal Cells ◽  
Nippostrongylus Brasiliensis ◽  
Normal Appearance

The cuticle of adults ofNippostrongylus brasiliensishas been described using histological, histochemical and ultrastructural techniques.The cuticle has the following layers: an outer triple-layered membrane; a single cortical layer; a fluid-filled layer which is traversed by numerous collagen fibrils; struts which support the fourteen longitudinal ridges of the cuticle and which are suspended by collagen fibrils in the fluid-filled layer; two fibre layers, each layer apparently containing three layers of fibres; and a basement lamella.The fluid-filled layer contains haemoglobin and esterase.The muscles of the body wall are attached to either the basement lamella or to the fibre layers of the cuticle.The mitochondria of the hypodermis are of normal appearance.The longitudinal ridges of the cuticle appear to abrade the microvilli of the intestinal cells of the host.Possible functions of the cuticle are discussed.I wish to thank Dr P. Tate, in whose department this work was done, for helpful suggestions and criticism at all stages of this work, and Mr A. Page for technical assistance. I also wish to thank Professor Boyd for permission to use the electron microscope in the Department of Anatomy.

Studies on the life-history of Bunostomum phlebotomum (Railliet, 1900), a hookworm parasite of cattle

Parasitology ◽  
1946 ◽  
Vol 37 (3-4) ◽  
pp. 192-201 ◽  
Author(s):  
J. F. A. Sprent
Keyword(s):  
Infective Larva ◽  
Stage Larva ◽  
Fourth Stage ◽  
Larval Stages ◽  
The Third ◽  
Third Stage ◽  
History Of ◽  
Time Required ◽  
Cutaneous Blood ◽  
Fourth Stage Larva

A description is given of the processes of copulation, formation of the egg and spermatozoon, cleavage, embryogeny and hatching in B. phlebotomum. These processes were found to be essentially similar to those in other strongyle nematodes.The anatomy of the first three larval stages is described and the observations of Conradi & Barnette (1908) and Schwartz (1924) were largely confirmed.Penetration of the skin of calves by the infective larva was observed histologically. The larvae were found to have reached the dermis within 30 min. and to have penetrated the cutaneous blood vessels within 60 min. of application to the skin. The larvae were found in the lung where the third ecdysis was in progress 10 days after penetration of the skin. A description is given of the growth of the third-stage larva in the lung, the changes which take place during the third ecdysis, and the anatomy of the fourth-stage larva.The fourth-stage larvae exsheath in the lungs and travel to the intestine. After a period of growth in which sexual differentiation takes place, the fourth ecdysis occurs and the adult parasite emerges. The time required for the attainment of maturity was found to be somewhere between 30 and 56 days after penetration of the skin.This paper was written at the Ministry of Agriculture and Fisheries Veterinary Laboratories, Wey-bridge, and the writer would like to express his gratitude to the Director, Prof. T. Dalling, also to Dr W. R. Wooldridge, chairman of the Council of the Veterinary Educational Trust for their help and encouragement. The writer's thanks are also due to Dr H. A. Baylis, Prof. R. T. Leiper and Dr E. L. Taylor for their advice and help on technical points, and to Mr R. A. O. Shonekan, African laboratory assistant, for his able co-operation.

Terapi Mandi Terhadap Pecandu Narkotika Di Pondok Pesantren Al-Qodir Cangkringan Sleman Yogyakarta

2020 ◽  
Vol 1 (2) ◽  
pp. 1
Author(s):  
Muslimah Muslimah
Keyword(s):  
Qualitative Research ◽  
Descriptive Analysis ◽  
Boarding School ◽  
The Body ◽  
Second Stage ◽  
The Third ◽  
Third Stage ◽  
Preparation Stage ◽  
Qualitative Descriptive ◽  
Bath Therapy

This study aims to determine how the process and benefits of bath therapy for narcotic addicts at Al-Qodir Islamic Boarding School, Cangkringan, Sleman Yogyakarta. This research is a qualitative research with two clients who undergo healing due to narcotics addiction. Methods of data collection by interview, observation, documentation. The method of checking the validity of the data used the "triangulation" technique. Data analysis using qualitative descriptive analysis. The results of this study describe that the process of bathing therapy carried out by narcotic addicts at the Al-Qodir Islamic boarding school goes through several stages. The first is the preparation stage, namely the therapist preparing the facilities, waking the narcotic addicts santri, and reading prayers into the bathroom. The second stage of implementation is the intention to bathe, perform ablution, and pour water all over the body. The third stage of closing is reading the prayer out of the bathroom, and giving suggestions from the therapist. Abstrak Penelitian ini bertujuan untuk mengetahui bagaimana proses dan manfaat terapi mandi terhadap pecandu narkotika di Pondok Pesantren Al-Qodir Cangkringan Sleman Yogyakarta. Penelitian ini merupakan penelitian kualitatif dengan subjek penelitian dua orang klien yang menjalani penyembuhan akibat pecandu narkotika. Metode  pengumpulan data dengan wawancara, observasi, dokumentasi. Metode pemeriksaan keabsahan data dengan tehnik “triangulasi”. Analisis data menggunakan analisis deskriptif kualitatif. Hasil penelitian ini mendeskripsikan bahwa proses terapi mandi yang dilakukan oleh para santri pecandu narkotika di pondok pesantren Al-Qodir ini melalui beberapa tahapan. Pertama tahap persiapan yaitu terapis menyiapkan sarana, membangunkan para santri pecandu narkotika, dan membaca do’a masuk kamar mandi. Tahap kedua pelaksanaan yaitu niat mandi, berwudhu, menyiramkan air ke seluruh tubuh. Tahap ketiga penutupan yaitu membaca do’a keluar kamar mandi, dan memberikan sugesti dari terapis.

New information about the third stage larva and larval habitat of Microdon (Chymophila) bruchi Shannon, 1927 (Diptera, Syrphidae) from Argentina

2019 ◽  
Vol 53 (45-46) ◽  
pp. 2833-2853
Author(s):  
Guillermo P. López-García ◽  
Menno Reemer ◽  
Guillermo Debandi ◽  
Ximo Mengual
Keyword(s):  
Larval Habitat ◽  
Stage Larva ◽  
The Third ◽  
New Information ◽  
Third Stage
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