scholarly journals Hospital infections in Spain. I.Staphylococcus aureus(1978–91)

1993 ◽  
Vol 110 (3) ◽  
pp. 533-542 ◽  
Author(s):  
A. Vindel ◽  
P. Trincado ◽  
MM. Martin De Nicolas ◽  
E. Gomez ◽  
C. Martin Bourgon ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Staphylococcus Aureus ◽  
Considerable Increase ◽  
Hospital Infections ◽  
Phage Group ◽  
Group Iii ◽  
The Past ◽  
Phage Types ◽  
Group I ◽  
Sporadic Cases

SUMMARYThis study was undertaken to determine the distribution of phage types ofStaphylococcus aureusisolates from hospital outbreaks or sporadic cases received in our laboratory during the past 14 years. The records for 15803 isolates from 55 Spanish hospitals have been analysed.In relation to sporadic isolates we have been able to detect the predominance of phage group I and non-typable staphylococcal strains. Since 1989, we have observed a considerable increase in hospital infection caused by methicillin-resistantS. aureusstrains which we could differentiate in to two groups; one belonging to phage group III (6/47/54/75/77/84/85) and other groups of non-typable strains which could be classified as phage group I-III after heat treatment (29/77/84) and with similar patterns by reverse typing (6/47/53/54/75/83A/84/85/W57/1030/18042).During 1990 and 1991, these strains have extended widely to at least six different autonomous regions creating an epidemic situation in Spain.

scholarly journals Staphylococcus aureusstrains associated with the hedgehog,Erinaceus europaeus

1965 ◽  
Vol 63 (2) ◽  
pp. 285-291 ◽  
Author(s):  
John M. B. Smith
Keyword(s):  
Staphylococcus Aureus ◽  
Trichophyton Mentagrophytes ◽  
Penicillin Resistance ◽  
Erinaceus Europaeus ◽  
Phage Group ◽  
Group Iii ◽  
Group I ◽  
Group Ii

1. Strains ofStaphylococcus aureuswere obtained from the nostrils of twenty-three of fifty-eight hedgehogs; the skin of thirty-eight of fifty-six hedgehogs; the paws of thirty-six of fifty-seven hedgehogs and the anus of six of eleven hedgehogs.2. Of 118 strains, 106 (90%) were typable with human staphylophages. Seventeen were phage group I, three phage group II, twenty-five phage group III, sixty-one were typable but unclassifiable into groups, and twelve were untypable.3. Male hedgehogs were more heavily infected than females, while all ages of hedgehogs appeared equally susceptible to infection.4. Of the 124 coagulase positive strains obtained, 107 (86·3%) were resistant to penicillin. Resistance to other antibiotics—chloramphenicol, streptomycin, tetracycline, erythromycin, celbenin—was not encountered.5. Thirty-three (83%) of forty strains produced β-lysin.6. Mites (Caparinia tripilis) and fungi (Trichophyton mentagrophytesvar.erinacei) did not appear to directly influence the carriage ofStaphylococcus aureuson the hedgehog skin.

scholarly journals The bacteriology of tropical pyomyositis in Uganda

1965 ◽  
Vol 63 (4) ◽  
pp. 517-524 ◽  
Author(s):  
W. D. Foster
Keyword(s):  
Staphylococcus Aureus ◽  
Pure Culture ◽  
Phage Typing ◽  
Hospital Infections ◽  
Phage Group ◽  
Nasal Carrier ◽  
Phage Types ◽  
Natural Characteristic ◽  
Group Ii ◽  
Tropical Pyomyositis

1. The bacteriology of the pus from 79 cases of tropical pyomyositis has been studied. In 74 cases Staphylococcus aureus was isolated in pure culture.2. Phage typing showed that 60% of the pyomyositis strains belonged to phage Group II as compared with 22% for miscellaneous hospital infections and 21% for nasal carrier strains.3. A high proportion of the Group II strains from pyomyositis were found to be penicillin resistant and it is thought that this is unlikely to be due to the use of penicillin therapeutically but is probably a natural characteristic of the strains.4. Other suggested aetiological factors in pyomyositis such as leptospirosis and syphilis have been investigated but no association has been found.5. The phage types of staphylococci found in Uganda have been compared with those reported from England and Australia and found not to be strikingly different.

PHAGE GROUPS AND ANTIBIOTIC SENSITIVITY OF STAPHYLOCOCCUS AUREUS ASSOCIATED WITH CYSTIC FIBROSIS OF THE PANCREAS

PEDIATRICS ◽  
1959 ◽  
Vol 24 (1) ◽  
pp. 40-42
Author(s):  
Fred E. Pittman ◽  
Calderon Howe ◽  
Louise Goode ◽  
Paul A. di Sant'Agnese
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Phage Type ◽  
Antibiotic Sensitivity ◽  
Phage Group ◽  
Staph Aureus ◽  
Group Iii

In this study, 198 strains of hemolytic, coagulase-positive Staph. aureus were recovered from 84 patients with cystic fibrosis of the pancreas and some of their relatives. The majority of the organisms fell into phage group III and were resistant in vitro to penicillin and other antibiotics. No single phage type seemed to be unduly prevalent in this group of patients with cystic fibrosis of the pancreas.

scholarly journals Staphylococcal food poisoning in the United Kingdom, 1969–90

1993 ◽  
Vol 110 (3) ◽  
pp. 519-531 ◽  
Author(s):  
A. A. Wieneke ◽  
D. Roberts ◽  
R. J. Gilbert
Keyword(s):  
Staphylococcus Aureus ◽  
United Kingdom ◽  
Food Poisoning ◽  
Food Hygiene ◽  
Milk Products ◽  
Group Iii ◽  
The United Kingdom ◽  
Sporadic Cases ◽  
Fish And Shellfish

SUMMARYBetween 1969 and 1990 strains ofStaphylococcus aureusfrom 359 outbreaks and sporadic cases of staphylococcal food poisoning in the United Kingdom were examined in the PHLS Food Hygiene Laboratory for the production of enterotoxin. In a number of instances the incriminated foods were also examined for the presence of enterotoxin. Strains from 79% of incidents produced enterotoxin A alone or together with another enterotoxin. The level ofS. aureuspresent in the foods ranged from no viableS. aureusdetected to 1.5 × 1010c.f.u./g with a median of 3.0 × 107c.f.u./g. Enterotoxin was detected in foods in the absence of viableS. aureusin only two outbreaks and in both cheese was the implicated food. Meat. poultry or their products were the vehicle in 75% of incidents with ham and chicken most frequently implicated. Other foods included fish and shellfish (7%) and milk and milk products (8%). Most contamination took place in the home followed by restaurants and shops. Seventy-one percent of the incident strains were lysed by phages of group III or I/III.

scholarly journals The egg yolk reaction ofStaphylococcus aureusisolated from burns

1964 ◽  
Vol 62 (2) ◽  
pp. 229-237 ◽  
Author(s):  
E. J. L. Lowbury ◽  
B. J. Collins
Keyword(s):  
Mercuric Chloride ◽  
Phage Type ◽  
Egg Yolk ◽  
Negative Reaction ◽  
Phage Group ◽  
Staph Aureus ◽  
Group Iii ◽  
Group I

Staph. aureusfrom burns of in-patients were tested for egg yolk reaction during three periods; in 1958 and in 1960 approximately 80 % of the strains gave a negative reaction (EY-), but in 1962 only 36 % of the strains were egg yolk negative.Staphylococci of phage group III were more commonly EY- than those of other groups isolated from burns. Within each of groups I and III, however, there were patterns predominantly EY- and others predominantly egg yolk positive (EY+); in group I the majority of strains isolated in 1960 were of phage type 52 and EY-, while those isolated in 1962 were predominantly of phage type 80 or related patterns which were always EY+.Most of the staphylococci in burns were resistant to penicillin, tetracycline and erythromycin; within groups I and III, the staphylococci which were EY- were also more commonly resistant than EY+ strains to these three antibiotics.Most of the staphylococci from burns were mercuric chloride resistant (presumptive epidemic strains); of the mercuric chloride sensitive staphylococci, the proportion of EY+ strains was greater than that of EY- strains.

scholarly journals Characterization of non-typable strains ofStaphylococcus aureusfrom cases of hospital infection

1987 ◽  
Vol 99 (1) ◽  
pp. 191-200 ◽  
Author(s):  
Ana Vindel ◽  
Cecilia Martín-Bourgon ◽  
Juan A. Saez-Nieto ◽  
Saez Nieto
Keyword(s):  
Heat Treatment ◽  
Staphylococcus Aureus ◽  
Thermal Shock ◽  
Hospital Infection ◽  
Phage Typing ◽  
Alternative Methods ◽  
Sporadic Cases

SUMMARYA high percentage of non-typable (NT)Staphylococcus aureusstrains was isolated in Spanish hospitals during 1984 and 1985. Several alternative methods of typing were employed to study these isolates. These were: phage-typing at 1000 × RTD, phage-typing after heat-treatment (48 °C), thermal shock (56 °C), reverse-typing and induction of additional phages. Using these methods the number of NT isolates was reducedby 60%. Best results were obtained with heat-treatment. Additional phages and reverse-typing were also useful.A scheme for the study of outbreaks and sporadic cases caused by NT strains is proposed using the methods described.

Evaluation of Fibrolytic Effect of Bauhinia purpurea L.

2019 ◽  
Vol 6 (1) ◽  
pp. 21-32
Author(s):  
Jeevan R. Dash ◽  
Tapas K. Sar ◽  
Rinku Buragohain ◽  
Indranil Samanta ◽  
Rahul Nanotkar
Keyword(s):  
Staphylococcus Aureus ◽  
Single Dose ◽  
Mammary Gland ◽  
Fibrous Tissue ◽  
Stem Bark ◽  
Oral Dosing ◽  
Group Iii ◽  
Mammary Gland Tissue ◽  
Group I ◽  
Bauhinia Purpurea

Background: Mammary gland tissue (left half) revealed the presence of excess proliferation of fibrous tissue with disorganization of alveolar structures and the right half showed extensive fibrous tissue proliferation of mammary gland following intramammary inoculation of 2000 c.f.u. of Staphylococcus aureus. However, oral dosing of Bauhinia variegata L. bark powder at 6 gm/kg for 7 days and 3 gm/kg for another 7 days exhibited reduction of fibrous tissue in chronic mastitis. Objective: The fibrolytic effect of one week oral dosing of Bauhinia purpurea L. bark powder was studied in chronic mastitis with induced fibrosis as Bauhinia variegate L. is rarely available in plain land. Methods: Chronic mastitis with fibrosis was induced by intramammary inoculation of coagulase positive Staphylococcus aureus in group III and IV goats. Group I and III goats were injected with a single dose of ceftriaxone at 20 mg/kg intravenously, whereas group II and IV goats were orally administered Bauhinia purpurea L. bark powder at 6 g/kg for 7 days with a single dose of ceftriaxone at 20 mg/kg intravenously. Results: The t1/2β of ceftriaxone with Bauhinia purpurea L. stem bark powder in chronic mastitis increased significantly. Ceftizoxime was detected in plasma from 1 h to 48 h post dosing (pd) in group III and from 1 h to 96 h pd in group IV, respectively. Conclusion: Bark powder of Bauhinia purpurea L. at 6g/kg orally once daily increased the bioavailability of ceftriaxone and or ceftizoxime in milk due to its fibrolytic effect which was not reported earlier. Therefore, the Bauhinia purpurea L. bark powder having fibrolytic effect has the potential to reduce development of cancer.

scholarly journals Staphylococcus aureus prevalence among hospitalized patients

Medicina ◽  
2008 ◽  
Vol 44 (8) ◽  
pp. 593
Author(s):  
Žaneta Pavilonytė ◽  
Renata Kaukėnienė ◽  
Aleksandras Antuševas ◽  
Alvydas Pavilonis
Keyword(s):  
Staphylococcus Aureus ◽  
Prevalence Rate ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Phage Type ◽  
Hospitalized Patients ◽  
Diffusion Method ◽  
Phage Group ◽  
Methicillin Resistant ◽  
Group Iii ◽  
Group Ii

Objective. To determine the prevalence of Staphylococcus aureus strains among hospitalized patients at the beginning of their hospitalization and during their treatment and the resistance of strains to antibiotics, and to evaluate epidemiologic characteristics of these strains. Patients and methods. Sixty-one patients treated at the Department of Cardiac, Thoracic and Vascular Surgery were examined. Identification of Staphylococcus aureus strains was performed using plasmacoagulase and DNase tests. The resistance of Staphylococcus aureus to antibiotics, b-lactamase production, phagotypes, and phagogroups were determined. The isolated Staphylococcus aureus strains were tested for resistance to methicillin by performing disc diffusion method using commercial discs (Oxoid) (methicillin 5 mg per disk and oxacillin 1 mg per disk). Results. A total of 297 Staphylococcus aureus strains were isolated. On the first day of hospitalization, the prevalence rate of Staphylococcus aureus strains among patients was 67.3%, and it statistically significantly increased to 91.8% on days 7–10 of hospitalization (P<0.05). During hospitalization, patients were colonized with Staphylococcus aureus strains resistant to cephalothin (17.6% of patients, P<0.05), cefazolin (14.6%, P<0.05), tetracycline (15.0%, P<0.05), gentamicin (37.7%, P<0.001), doxycycline (30.7%, P<0.001), and tobramycin (10.6%, P>0.05). Three patients (4.9%) were colonized with methicillin-resistant Staphylococcus aureus strains, belonging to phage group II phage type 3A and phage group III phage types 83A and 77; 22.6– 25.5% of Staphylococcus aureus strains were nontypable. During hospitalization, the prevalence rate of phage group II Staphylococcus aureus strains decreased from 39.6% to 5.7% (P<0.05) and the prevalence rate of phage group III Staphylococcus aureus strains increased to 29.5% (P<0.001). Conclusions. Although our understanding of Staphylococcus aureus is increasing, well-designed communitybased studies with adequate risk factor analysis are required to elucidate further the epidemiology of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. Surveillance of methicillin-resistant Staphylococcus aureus provides relevant information on the extent of the methicillin-resistant Staphylococcus aureus epidemic, identifies priorities for infection control and the need for adjustments in antimicrobial drug policy, and guides intervention programs.

scholarly journals Studies on transmission of Staphylococcus aureus in an isolation ward for burned patients

1973 ◽  
Vol 71 (1) ◽  
pp. 171-183 ◽  
Author(s):  
Anna Hambraeus
Keyword(s):  
Staphylococcus Aureus ◽  
Phage Group ◽  
Burned Patient ◽  
Staph Aureus ◽  
Group Iii ◽  
Service Areas ◽  
One Year ◽  
Isolation Ward ◽  
Very High ◽  
Made In

SUMMARYA one-year epidemiological investigation was made in an isolation ward for burned patients. The transmission of Staphylococcus aureus was mainly studied. In spite of the design of the ward the cross-infection rate was high. In all, 49 of 69 patients were infected 114 times. Twenty-six of the strains causing infection were found in a patient only, 10 in a member of the staff only and 23 in both patients and staff the week before they caused a new infection. There were three epidemic outbreaks caused by three strains of Staph. aureus all belonging to phage group III; one was resistant to methicillin. Environmental studies with settle plates showed that the number of staphylococci dispersed by a burned patient was often very high. In 8% of the observations in occupied bedrooms the air count of Staph. aureus was more than 1800 col./m.2 hr. However, the counts of Staph. aureus in the corridor and service areas were low. This seems to indicate a rather good protection against airborne transfer of bacteria. Other routes of infection were probably of greater importance.

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