scholarly journals A foodborne outbreak of Campylobacter jejuni (O[ratio ]33) infection associated with tuna salad: a rare strain in an unusual vehicle

1998 ◽  
Vol 121 (2) ◽  
pp. 281-287 ◽  
Author(s):  
T. H. ROELS ◽  
B. WICKUS ◽  
H. H. BOSTROM ◽  
J. J. KAZMIERCZAK ◽  
M. A. NICHOLSON ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Summer Camp ◽  
Food Product ◽  
Raw Milk ◽  
Food Handler ◽  
Recreational Water ◽  
Foodborne Outbreak ◽  
Serotype O ◽  
Stool Specimens ◽  
Control Study

We report a foodborne outbreak of Campylobacter jejuni infection in a summer camp. Outbreak-related cases occurred in 79 persons including 3 secondary cases in campers. Campylobacter jejuni was isolated from stool specimens from 16 of 21 patients who submitted a sample; 13 viable isolates were serotyped and all were serotype O[ratio ]33 (somatic O scheme) or HL[ratio ]18 (heat-labile scheme), and biotype III (Lior scheme). This serotype is widely distributed geographically but rarely isolated from humans. Samples of water from the wells supplying the camp were negative for faecal coliforms, and raw milk had not been served in the camp. A matched (1[ratio ]1) case-control study identified tuna salad served for lunch on 19 July as the likely food item associated with illness (matched odds ratio=22; 95% confidence intervals (CI)=3·6–908). Swimming in the camp pool and other recreational water use in area lakes by the campers were not statistically associated with illness. The precise mechanism of introduction of the organism into the tuna salad remains unknown; contamination most likely occurred through cross-contamination with another food product, the hands of a food handler, or a work surface. Several deficiencies in the operation of the camp kitchen were identified. In Wisconsin, kitchens of such camps are subject to different inspection rules than restaurants. Camp staff, administrators, counselors, food managers, and infirmary staff, should fulfil important roles in their respective areas to prevent future outbreaks.

scholarly journals Gastrointestinal infections caused by consumption of raw drinking milk in England & Wales, 1992–2017

2019 ◽  
Vol 147 ◽  
Author(s):  
N. Adams ◽  
L. Byrne ◽  
J. Edge ◽  
A. Hoban ◽  
C. Jenkins ◽  
...  
Keyword(s):  
Public Health ◽  
Infectious Disease ◽  
Campylobacter Jejuni ◽  
Raw Milk ◽  
National Surveillance ◽  
Gastrointestinal Infections ◽  
Food Standards ◽  
The Uk ◽  
Drinking Milk ◽  
Intestinal Infectious Disease

Abstract Systematic, national surveillance of outbreaks of intestinal infectious disease has been undertaken by Public Health England (PHE) since 1992. Between 1992 and 2002, there were 19 outbreaks linked to raw drinking milk (RDM) or products made using raw milk, involving 229 people; 36 of these were hospitalised. There followed an eleven-year period (2003–2013) where no outbreaks linked to RDM were reported. However, since 2014 seven outbreaks of Escherichia coli O157:H7 (n = 3) or Campylobacter jejuni (n = 4) caused by contaminated RDM were investigated and reported. Between 2014 and 2017, there were 114 cases, five reported hospitalisations and one death. The data presented within this review indicated that the risk of RDM has increased since 2014. Despite the labelling requirements and recommendations that children should not consume RDM, almost a third of outbreak cases were children. In addition, there has been an increase in consumer popularity and in registered RDM producers in the UK. The Food Standards Agency (FSA) continue to provide advice on RDM to consumers and have recently made additional recommendations to enhance existing controls around registration and hygiene of RDM producers.

scholarly journals Draft Genome Sequences of Three Strains of Campylobacter jejuni Isolated from Patients with Guillain-Barré Syndrome in Bangladesh

2021 ◽  
Vol 10 (17) ◽  
Author(s):  
Zhahirul Islam ◽  
Fahmida Habib Nabila ◽  
Asaduzzaman Asad ◽  
Ruma Begum ◽  
Israt Jahan ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Draft Genome ◽  
Guillain Barre Syndrome ◽  
Genome Sequences ◽  
Content Type ◽  
Guillain Barré Syndrome ◽  
Stool Specimens ◽  
Guillain Barré

ABSTRACT Campylobacter jejuni is the pathogen most commonly associated with Guillain-Barré syndrome (GBS). The present work describes the draft genome sequences of 3 C. jejuni strains, BD39, BD67, and BD75, isolated from stool specimens of patients with C. jejuni-triggered GBS using Illumina technologies.

scholarly journals Listeria monocytogenes: An emerging food-borne pathogen and its public health implications

2016 ◽  
Vol 10 (02) ◽  
pp. 149-154 ◽  
Author(s):  
Waffa W Reda ◽  
Khaled Abdel-Moein ◽  
Ahmed Hegazi ◽  
Yasmin Mohamed ◽  
Khaled Abdel-Razik
Keyword(s):  
Listeria Monocytogenes ◽  
Raw Milk ◽  
Food Samples ◽  
Food Borne ◽  
Contaminated Food ◽  
Luncheon Meat ◽  
Stool Specimens ◽  
Polymerase Chain ◽  
Minced Meat

Introduction: Listeria monocytogenes is considered one of the most important food-borne pathogens transmitted to humans via contaminated food. The aim of the present study was to demonstrate the importance of L. monocytogenes as a food-borne pathogen. Methodology: A total of 340 samples were collected from different localities in El Giza Governorate, Egypt, to check the occurrence of L. monocytogenes in that area. The collected samples comprised 250 food samples, 40 swabs from food refrigerators, and 50 stool specimens from diarrheic children. L. monocytogenes was isolated from the examined samples according to the International Organization for Standardization. The isolates were tested biochemically using Listeria Microbact 12L and confirmed by polymerase chain reaction. Results: The isolation rates of L. monocytogenes were 8% in beef burger, 4% in minced meat, 4% in luncheon meat, while sausage samples were all negative. Eight percent of raw milk samples were positive for L. monocytogenes, whereas cheese samples and refrigerator swabs were negative. Only Listeria grayi was isolated from human stools (2.5%). Conclusion: The high isolation rates of L. monocytogenes among the examined food stuffs highlight the crucial role of food as an important vehicle for this pathogen. More efforts should be made to ensure safe handling and processing of these foods to reduce the transmission of L. monocytogenes to humans.

scholarly journals Acceptance of Santo Giorno cheese typical of the Southwestern region of Paraná, Brazil

2017 ◽  
Vol 47 (4) ◽  
Author(s):  
Edimir Andrade Pereira ◽  
Roberta Roncatti ◽  
Carla Todescatto ◽  
Simone Beux ◽  
João Francisco Marchi ◽  
...  
Keyword(s):  
Inverse Relationship ◽  
Multinomial Logistic Regression ◽  
Food Product ◽  
Raw Milk ◽  
Acceptance Rate ◽  
Good Reproducibility ◽  
Plot Analysis ◽  
Typical Food ◽  
Box Plot ◽  
Effect Of Age

ABSTRACT: Santo Giorno cheese, obtained from raw milk and selected autochthonous starters, is emerging as the newest typical food product from the Southwestern region of Paraná, Brazil. The objective of this study was to evaluate the acceptance of the cheese with two ripening times of 60 and 180 days, produced in two dairy factories, testing two starters and two preservatives. Subjective sensory evaluation was applied using 129 consumers and hedonic scales for the attributes and for purchasing intent. A questionnaire involving the rate of cheese consumption was also used. The results suggested good reproducibility of the cheese preparation by the dairy factories, with no significant differences between the type of preservative used and the suitability of the two types of starter tested. An inverse relationship between hedonic scores for attributes (appearance, color, odor, texture, flavor) and ripening time was observed, except for texture. By applying multinomial logistic regression and a box plot analysis, a significant effect of age on cheese purchasing intent was verified, with a mean acceptance rate of 87.8% for 60 days ageing and 81.8% for 180 days ageing. Results revealed a possible Santo Giorno cheese public of older consumers who had a cheese consuming habit.

Evaluation of the Bacteriological Health Risk of 60-Day Aged Raw Milk Cheddar Cheese

1984 ◽  
Vol 47 (7) ◽  
pp. 530-531 ◽  
Author(s):  
MICHAEL H. BRODSKY
Keyword(s):  
Staphylococcus Aureus ◽  
Alkaline Phosphatase ◽  
Health Risk ◽  
Campylobacter Jejuni ◽  
Alkaline Phosphatase Activity ◽  
Geometric Mean ◽  
Cheddar Cheese ◽  
Raw Milk ◽  
Fecal Coliforms ◽  
Salmonella Spp

One hundred twenty-seven 60-d aged Cheddar cheese samples produced by 21 provincially inspected cheese plants were analyzed by 8 regional laboratories of the Ontario Ministry of Health. Coliforms were detected in 37 (31.2%) and fecal coliforms confirmed in 22 (18.3%) samples, with geometric mean counts per g of 92.5 and 79.3, respectively. Staphylococcus aureus was found in only two products at a level of >1000 per g. Salmonella spp. and Campylobacter jejuni were not isolated from any of the samples tested. Yersinia enterocolitica was isolated from one product; however, the isolate was bile esculin-and salicin-positive, and considered a non-pathogenic biotype. The pH of these aged Cheddars ranged between 4.98 and 5.50, with a mean of 5.26. Alkaline phosphatase activity was detected in 94 (79.7%) of the 118 samples tested. These results suggest that 60-d aged raw milk Cheddar cheese produced in Ontario does not pose a significant bacteriological health risk.

scholarly journals Epidemiological and Genomic Characterization of Campylobacter jejuni Isolates from a Foodborne Outbreak at Hangzhou, China

2020 ◽  
Vol 21 (8) ◽  
pp. 3001 ◽  
Author(s):  
Hua Yu ◽  
Mohammed Elbediwi ◽  
Xiaohong Zhou ◽  
Huiqun Shuai ◽  
Xiuqin Lou ◽  
...  
Keyword(s):  
Real Time ◽  
Campylobacter Jejuni ◽  
Vibrio Parahaemolyticus ◽  
Genetic Difference ◽  
Public Health Problem ◽  
Foodborne Outbreak ◽  
Study Results ◽  
Significant Public Health ◽  
Selection Medium ◽  
Bacterial Selection

Background: Foodborne outbreaks caused by Campylobacter jejuni have become a significant public health problem worldwide. Applying genomic sequencing as a routine part of foodborne outbreak investigation remains in its infancy in China. We applied both traditional PFGE profiling and genomic investigation to understand the cause of a foodborne outbreak in Hangzhou in December 2018. Method: A total of 43 fecal samples, including 27 sick patients and 16 canteen employees from a high school in Hangzhou city in Zhejiang province, were recruited. Routine real-time fluorescent PCR assays were used for scanning the potential infectious agents, including viral pathogens (norovirus, rotavirus, adenovirus, and astrovirus), and bacterial pathogens (Salmonella, Shigella, Campylobacter jejuni, Vibrio parahaemolyticus and Vibrio cholerae). Bacterial selection medium was used to isolate and identify the positive bacteria identified by molecular test. Pulsed field gel electrophoresis (PFGE), and next generation sequencing (NGS) were applied to fifteen recovered C. jejuni isolates to further understand the case linkage of this particular outbreak. Additionally, we retrieved reference genomes from the NCBI database and performed a comparative genomics analysis with the examined genomes produced in this study. Results: The analyzed samples were found to be negative for the queried viruses. Additionally, Salmonella, Shigella, Vibrio parahaemolyticus and Vibrio cholera were not detected. Fifteen C. jejuni strains were identified by the real-time PCR assay and bacterial selection medium. These C. jejuni strains were classified into two genetic profiles defined by the PFGE. Out of fifteen C. jejuni strains, fourteen have a unified consistent genotype belonging to ST2988, and the other strain belongs to ST8149, with a 66.7% similarity in comparison with the rest of the strains. Moreover, all fifteen strains harbored blaOXA-61 and tet(O), in addition to a chromosomal mutation in gyrA (T86I). The examined fourteen strains of ST2988 from CC354 clone group have very minimal genetic difference (3~66 SNPs), demonstrated by the phylogenomic investigation. Conclusion: Both genomic investigation and PFGE profiling confirmed that C. jejuni ST2988, a new derivative from CC354, was responsible for the foodborne outbreak Illustrated in this study.

Methods for Recovery of Campylobacter jejuni from Foods

1982 ◽  
Vol 45 (14) ◽  
pp. 1332-1337 ◽  
Author(s):  
NORMAN J. STERN
Keyword(s):  
Campylobacter Jejuni ◽  
Gas Flow ◽  
Raw Milk ◽  
Growth Conditions ◽  
Food Samples ◽  
Human Infection ◽  
Animal Products ◽  
Enrichment Broth ◽  
Selective Media ◽  
Sensitivity And Selectivity

The triangular relationship between Campylobacter jejuni, foods and disease in humans has been well-documented. Many studies have revealed that C. jejuni causes at least as many cases of human gastroenteritis as does Salmonella sp. Foods are an important vehicle in human infection, and raw milk is most frequently implicated. Other animal products also serve as potential sources of infection. C. jejuni has been found on the carcasses of poultry and other domestic animals throughout the world. The organism is microaerophilic and various methods for establishing appropriate growth conditions, such as the Fortner principle, atmosphere replacement and adding of supplements to encourage growth of C. jejuni, are available. Methods developed for use in clinical laboratories lack the necessary sensitivity and selectivity, and therefore have limited use in detecting small numbers of C. jejuni in foods. In one enrichment method for detecting C. jejuni in foods, washings are filtered and centrifuged, the sediment is suspended in the enrichment broth and the suspension is incubated under a constant gas flow at reduced oxygen levels. Following incubation enrichment broth is filtered and plated onto selective media. In another recently developed method, food samples are directly added to an enrichment broth with antibiotics and incubated under a microaerobic atmosphere before selective plating. Butzler's, Skirrow's and Campy-BAP selective media use several antibiotics to which C. jejuni is resistant. The plates are supplemented with horse or sheep blood, depending upon the specific formulation. The optimum temperature for growth of C. jejuni, about 42°C, may also be used for selection. It is now possible to recover 0.1 to 1 cell of C. jejuni per 10 to 25 g of food sample from among 106 to 109 indigenous bacteria. After a characteristic colony is isolated, the key criteria for presumptive identification of C. jejuni by phase-contrast microscopy are darting, corkscrew motion and a comma to spiral shape.

Sign in / Sign up

Export Citation Format

Share Document