The effect of maturity on the moisture relations of seed longevity in foxglove (Digitalis purpurea L.)

1997 ◽  
Vol 7 (4) ◽  
pp. 341-350 ◽  
Author(s):  
F.R. Hay ◽  
R.J. Probert ◽  
R. D. Smith
Keyword(s):  
Moisture Content ◽  
Seed Development ◽  
Dry Weight ◽  
Seed Longevity ◽  
Fresh Weight Basis ◽  
Digitalis Purpurea ◽  
Long Term Storage ◽  
Logarithmic Relationship ◽  
Term Storage

AbstractControlled aging experiments were carried out in order to evaluate the changes in seed longevity occurring following the attainment of maximum seed dry weight in foxglove (Digitalis purpurea L.). Seeds harvested in three successive years were stored at ~5% moisture content (fresh weight basis) and 50°C. Seed longevity was greater the later the seeds were harvested. When the data were modelled using the predictive viability model of Ellis and Roberts, these increases were attributable to increases in both the theoretical initial viability (in probits) of the seed-lot, Ki, and in the standard deviation of the normal distribution of seed deaths in time, σ. Furthermore, there was a positive relationship between Ki and σ which was independent of the year of harvest (r = 0.9016, for 11 d.f., P < 0.001). These results do not support the predictive model which relies on the assumption that σ will not differ between seed lots of the same species stored under identical conditions. When seeds were stored at a range of moisture contents (between 5 and 10%) at 50°C, increases in σ during seed development were manifest as changes in the negative logarithmic relationship between σ and moisture content; below an upper limit, the regressions for seed lots at different stages of maturity were significantly different (P < 0.05) but could be constrained to either a common intercept or to a common slope (P < 0.05). It is proposed that it was the inherent variation in individual seed lifespans which increased during seed development. These results raise important concerns regarding the use of predetermined ‘species constants’ to predict the longevity of seed lots during long-term storage in seed banks.

scholarly journals The Technological Investigations Of Manufacturing Of Energy Chips

2015 ◽  
Vol 1 ◽  
pp. 220
Author(s):  
Ziedonis Miklašēvičs
Keyword(s):  
Moisture Content ◽  
Dry Mass ◽  
Quality Parameters ◽  
Heating Value ◽  
Long Term Storage ◽  
Lower Heating Value ◽  
Term Storage ◽  
Lower Heating

The methodology in Latvia forest industry provide to determine the quality of energy chips only in long- term storage places before selling. Due to the lack of hard empirical data about the quality parameters of energy chips in different phases of manufacturing process, this research paper consists of: - the identification and analyses of the factors that influenced the values of energy chips quality features such as: bulk density, moisture content, ash content, higher and lower heating value according to actual moisture content and per dry mass of the chips; - the methodology for determination the quality parameters of energy chips by analysis the wood moisture content and by choice the method of the manufacturing of energy chips.

The climatic potential for field hay drying in north-west Europe

1985 ◽  
Vol 105 (1) ◽  
pp. 167-181 ◽  
Author(s):  
N. Thompson ◽  
I. A. Barrie ◽  
L. D. J. Harvey
Keyword(s):  
Meteorological Data ◽  
Objective Assessment ◽  
Dry Weight ◽  
Data Sets ◽  
North West ◽  
Long Term Storage ◽  
Average Rainfall ◽  
Term Storage ◽  
Continental Climate

SUMMARYA model of a drying swath has been developed and used with 20-year long meteorological data sets from 32 sites in north-west Europe to provide an objective assessment of the influence of weather on field hay drying. The model considered the swath in terms of its bulk properties but incorporated the effects on swath drying of soil evaporation and stubble transpiration: delays in swath drying caused by interception of rain or dew were calculated explicitly.A cutting season from May to September was assumed, and results given in terms of the number of daily cuts reaching various moisture contents in less than 10 days, and especially those reaching 25% (dry-weight basis) within 5 days so that dry-matter losses were relatively small, and immediate long-term storage was possible. Rainfall amounts on the faster-drying swaths were usually less than 1 mm. A large inter-annual variability in hay-drying prospects was demonstrated, even at sites with fairly good climates for drying on average. The most favourable conditions for field drying were shown to occur substantially earlier in the cutting season at maritime sites than at those with a more continental climate. Conditioning of the swath allowed more cuts to dry to a prescribed moisture content, but with the penalty of larger average rainfall on the swath, and consequent loss of quality.

Critical review of threatened species collections in the New South Wales Seedbank: implications for ex situ conservation of biodiversity

2004 ◽  
Vol 10 (4) ◽  
pp. 221 ◽  
Author(s):  
C. A. Offord ◽  
M. L. McKensy ◽  
P. V. Cuneo
Keyword(s):  
Moisture Content ◽  
Threatened Species ◽  
New South ◽  
Ex Situ ◽  
Seed Moisture Content ◽  
Long Term Storage ◽  
South Wales ◽  
Seed Moisture ◽  
Term Storage

This article reviews the germinability and viability of seeds of threatened species collections in the New South Wales (NSW) Seedbank with the manifold aims of: ensuring that existing storage treatments and conditions provide effective ex situ storage of threatened species seed; providing baseline viability and seed storage life data on threatened species; and, identifying research gaps in seed germination and storage protocols for threatened species and communities. The germinability and viability of a range of seed accessions, of various ages and stored under different (although mainly identifiable) conditions in the NSW Seedbank, was determined through germination and cut-tests. The results indicated that many of the Fabaceae, Myrtaceae and Proteaceae species tested are orthodox and can be stored at 5 to 10% moisture content at 5�C for up to ten years without significant loss of viability (short- to medium-term storage). The best results were obtained in the lower seed moisture content range (2 to 9%), which appeared to be especially critical for long-term storage of many Proteaceae accessions, reinforcing the need to attain the correct seed moisture content for long-term storage. Around 10% of accessions exhibited some degree of dormancy even after long storage periods. Storage of the widest range of species, for periods greater than ten years for long-term conservation purposes, is generally best conducted by storing at sub-zero temperatures. Freezing at -18�C had little effect on the germinability of a range of seeds tested and is recommended over storage at 5�C. Collection and seed banking procedures for the NSW Seedbank will be regularly reviewed and procedures modified in order to identify the best long-term storage conditions for species within this and other seedbanks. Seed collection strategies to maximize diversity and uses of seedbanks in conservation are discussed.

Moisture Content of Baled Forest and Urban Woody Biomass during Long-term Open Storage

2018 ◽  
Vol 34 (1) ◽  
pp. 225-230
Author(s):  
James H Dooley ◽  
Matthew J Wamsley ◽  
Jason M Perry
Keyword(s):  
Moisture Content ◽  
Pacific Northwest ◽  
Initial Moisture Content ◽  
Woody Biomass ◽  
Dry Weight ◽  
The United States ◽  
Long Term Storage ◽  
The Pacific ◽  
Early Fall

Abstract. This article describes how the moisture content of baled woody biomass varied during long-term open-air storage under conditions in the Pacific Northwest region of the United States. Large rectangular bales of forest and urban biomass were produced beginning in August 2015 and periodically until June 2016. Weights were measured approximately monthly until the entire lot of bales was ground into fuel in December 2016. Because it was impractical to non-destructively obtain moisture content samples during the study, final moisture content was measured from the ground material. Estimated temporal moisture contents were back-calculated from the final dry weight and moisture content of bales. All bales dried considerably during the spring and summer months, achieving a minimum moisture content in the early fall of 15 to 29% (wb). Minimum early fall moisture content had no correlation with the initial moisture content at time of baling. The ending moisture content in December 2016 ranged from 44% to 57% (wb), with a mean moisture content of 53% (wb). Ending moisture content had no correlation with initial moisture content at the time of baling, but appeared to be related to the amount of fine versus coarse woody material in the bales. Bales of forest and urban woody biomass proved to be structurally stable during long-term storage to enable handling and final transport to a centralized grinding location. Keywords: Density, Logging, Baling, Bioenergy, Biofuel, Bundling, Forest operations, Forestry, MC, Seasonality, Transport, Woody biomass.

scholarly journals Effects of moisture content, storage temperature and type of storage bag on the germination and viability of stored European beech (Fagus sylvatica L.) seeds

2008 ◽  
Vol 54 (No. 7) ◽  
pp. 287-293 ◽  
Author(s):  
Z. Procházková ◽  
L. Bezděčková
Keyword(s):  
Moisture Content ◽  
Fagus Sylvatica ◽  
Storage Temperature ◽  
European Beech ◽  
Fresh Weight Basis ◽  
Factorial Anova ◽  
Long Term Storage ◽  
Polyethylene Bags ◽  
Technical Rules

Beechnuts from three <I>Fagus sylvatica</I> lots were dried at 20°C to 5–6% or 8–9% (fresh weight basis) moisture content and stored at –7°C or –22°C for 3.5 or 4.5 years in sealed polyethylene bags containing air and routinely used for the long-term storage of beechnuts or vacuumed (de-aerated) polyethylene bags with air removed before sealing. The germination and viability (tetrazolium test) of beechnuts were determined according to the Czech Technical Rules (1997) once a year during storage. The results were subjected to factorial ANOVA and the means were compared using the Scheffe test at <I>P</I> > 0.05. Compared to beechnuts dried to an 8–9% moisture content a significant decrease in both germination and viability occurred in beechnuts dried to a 5–6% moisture content regardless of how long the beechnuts were stored. However, the effects of the other treatments (type of storage bag, storage temperature) were not significant.

scholarly journals The Influence of Moisture Content and Temperature on the Long-Term Storage Stability of Freeze-Dried High Concentration Immunoglobulin G (IgG)

Pharmaceutics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 303 ◽  
Author(s):  
Arnold Duralliu ◽  
Paul Matejtschuk ◽  
Paul Stickings ◽  
Laura Hassall ◽  
Robert Tierney ◽  
...  
Keyword(s):  
Moisture Content ◽  
Immunoglobulin G ◽  
Storage Stability ◽  
Binding Activity ◽  
Atmospheric Environment ◽  
Long Term Storage ◽  
Freeze Dried ◽  
Monomer Content ◽  
Term Storage

High protein concentration products for targeted therapeutic use are often freeze-dried to enhance stability. The long-term storage stability of freeze-dried (FD) plasma-derived Immunoglobulin G (IgG) from moderate to high concentrations (10–200 mg/mL) was assessed. Monomer content, binding activity and reconstitution times were evaluated over a 12-month period under accelerated and real-term storage conditions. In the first case study it was shown that FD IgG from 10 to 200 mg/mL had minimal monomer/activity losses at up to ambient temperature after 12 months of storage. However, at 45 °C the sucrose-to-protein ratio played a significant impact on IgG stability above 50 mg/mL. All IgG concentrations witnessed moisture ingress over a 12-month period. The impact of moisture ingress from environmental exposure (between 0.1% and 5% w/w moisture) for IgG 50 mg/mL was assessed, being generated by exposing low moisture batches to an atmospheric environment for fixed time periods. Results showed that at −20 °C and 20 °C there was no significant difference in terms of monomer or antigen-binding activity losses over 6 months. However, at 45 °C, there were losses in monomer content, seemingly worse for higher moisture content samples although model binding activity indicated no losses. Finally, the difference between a low moisture product (0.1–1% w/w) and a moderately high moisture (3% w/w) product generated by alternative freeze-drying cycles, both stoppered under low oxygen headspace conditions, was evaluated. Results showed that at −20 °C and 20 °C there was no difference in terms of binding activity or monomer content. However, at 45 °C, the low moisture samples had greater monomer and binding activity losses than samples from the highest moisture cycle batch, indicating that over-drying can be an issue.

scholarly journals Influence of dehydration on cryopreservation of Musa spp. germplasm

2020 ◽  
Vol 79 (2) ◽  
pp. 99-104
Author(s):  
Ergun Kaya ◽  
Fernanda Vidigal Duarte Souza ◽  
Janay Almeida dos Santos-Serejo ◽  
Selin Galatali
Keyword(s):  
Seed Germination ◽  
Laminar Flow ◽  
Moisture Content ◽  
Liquid Nitrogen ◽  
Zygotic Embryos ◽  
Long Term Storage ◽  
Nitrogen Treatment ◽  
Term Storage ◽  
Laminar Flow Cabinet

Cryopreservation is an important technique for the long-term storage of economically important plant germplasm. In this study, an efficient protocol was developed for the long-term conservation of seven economically important Musa taxa: M. acuminata Colla ssp. burmannica N.W. Simmonds, M. acuminata Colla ssp. zebrina (Van Houtte) R.E. Nasution, M. balbisiana Colla, M. basjoo Sieb., M. ornata W. Roxburgh (St. Lavender), M. velutina H. Wendl. et Drude (Velvet Pink Banana), and M. acuminata’ balbisiana. The seeds were dehydrated in a sterile laminar flow cabinet for different exposure times and then they were directly immersed in liquid nitrogen. The critical point was to support the initial germination of cryopreserved seeds and this was achieved by the excision of zygotic embryos after liquid nitrogen treatment that allowed the seed germination. The best moisture content for tolerance to cryopreservation ranged from 15.8% (M. acuminata ssp. zebrina) to 17.1% (M. ornata) and the maximum post-cryopreservation germination rates varied from 86.4% (M. velutina) to 55.0% (M. ornata). All seedlings derived from seeds germinated after cryopreservation were easily rooted and acclimated to greenhouse conditions.

Cryopreservation of Fagus crenata seeds: estimation of optimum moisture content for maintenance of seed viability by Bayesian modeling

2018 ◽  
Vol 48 (2) ◽  
pp. 192-196 ◽  
Author(s):  
Keita Endoh ◽  
Michinari Matsushita ◽  
Megumi K. Kimura ◽  
So Hanaoka ◽  
Yuko Kurita ◽  
...  
Keyword(s):  
Moisture Content ◽  
Seed Viability ◽  
Fagus Crenata ◽  
Natural Forests ◽  
Fagus Crenata Blume ◽  
Long Term Storage ◽  
High Viability ◽  
Term Storage ◽  
Seed Cryopreservation

Fagus crenata Blume is an important forestry tree species that plays a key biological role in natural forests. Because F. crenata forests are declining under global warming, the conservation of seed resources is necessary to maintain sustainable regeneration. We examined the capability of seed cryopreservation to achieve semi-permanent preservation of the F. crenata genetic resource. Seeds at 7%–26% moisture content (MC) exhibited greater than 64% viability, with developing radicles growing at 22 °C in 16 h photoperiods following chilling at 2 °C for 2 months. These seeds were then stored cryogenically at –170 °C for 6 months; cryopreservation at 26% MC reduced seed viability to 12%, whereas dry seeds (7%–14% MC) maintained viability and were comparable with seeds that had neither been dried nor cryopreserved. Drying seeds to 6% MC reduced viability to 47%; following cryopreservation, the viability of these seeds with such a low MC was 36%. Using a Bayesian model, we then determined that the optimal range of MC for the cryopreservation of F. crenata seeds was 9%–12%. We conclude that cryopreservation is feasible for the long-term storage of F. crenata seeds, drying of the seeds to the range of optimal MC retains high viability during cryogenic storage.

Sign in / Sign up

Export Citation Format

Share Document