scholarly journals Diagnosi di sesso e morfologia dei nuclei leucocitari: studio in campo normale e patologico

1959 ◽  
Vol 8 (S2) ◽  
pp. 139-142
Author(s):  
G. Sansone
Keyword(s):  
Sex Determination ◽  
Neonatal Life ◽  
Diagnostic Importance ◽  
Monozygous Twins ◽  
Made In

SUMMARYThe experience of the A. on the « genetical » sex determination (according to the method of Davidson e Smith) is summarized. It is stressed that: a) « drumsticks » in the female newborns are remarkably increased - b) in monozygous twins there is a strict similarity in the number of the « drumsticks » contrary to the cases of dizygous twins.The results obtained in cases of gonadal disgenesis are reported: of 16 patients with somatic female appearance 12 were cromatine-negative. The diagnostic importance of genetical sex determination in suspected cases of « Status Bonnevie-Ullrich » of the infants is outlined, In a reported case the diagnos's was made in the neonatal life.

The molecular biology of temperature-dependent sex determination

1995 ◽  
Vol 350 (1333) ◽  
pp. 297-304 ◽  
Keyword(s):  
Sex Determination ◽  
Molecular Basis ◽  
Current Knowledge ◽  
Temperature Dependent ◽  
The Past ◽  
Egg Incubation ◽  
Heteromorphic Sex Chromosomes ◽  
Substantial Progress ◽  
Common Molecular Basis ◽  
Made In

Many reptiles do not have heteromorphic sex chromosomes and for these species sex is determined during embryogenesis by the temperature of egg incubation rather than at conception. The phenomenon of temperature- dependent sex determination (TSD) was discovered almost thirty years ago, but few advances have been made towards the elucidation of its mechanism. In the past few years substantial progress has been made in the understanding of the molecular basis of XY chromosomal (genetic) sex determination (GSD) through the discovery of SRY. It is now possible to start comparing TSD with GSD. TSD is found in some evolutionarily ancient vertebrates and has been postulated to be the ancestral process from which GSD has evolved. If this is true then the two mechanisms may share a common molecular basis. This paper details the current knowledge of GSD, our progress on the investigation of the involvement of SRY-type proteins, and finally presents some of the problems that need to be resolved to gain an understanding of the molecular basis of TSD.

scholarly journals VaAPRT3 Gene is Associated With Sex Determination in Vitis amurensis

2021 ◽  
Vol 12 ◽  
Author(s):  
Yan Men ◽  
Ji-Rui Li ◽  
Hai-Lin Shen ◽  
Yi-Ming Yang ◽  
Shu-Tian Fan ◽  
...  
Keyword(s):  
Sex Determination ◽  
Vitis Amurensis ◽  
Cloning And Sequencing ◽  
Breeding Programs ◽  
Organ Differentiation ◽  
Gene Coding ◽  
Sex Marker ◽  
Sexual Identification ◽  
Determination Mechanism ◽  
Made In

In the past decade, progress has been made in sex determination mechanism in Vitis. However, genes responsible for sexual differentiation and its mechanism in V. amurensis remain unknown. Here, we identify a sex determination candidate gene coding adenine phosphoribosyl transferase 3 (VaAPRT3) in V. amurensis. Cloning and sequencing of the VaAPRT3 gene allowed us to develop a molecular marker able to discriminate female individuals from males or hermaphrodites based on a 22-bp InDel. Gene expression and endogenous cytokinin content analysis revealed that the VaAPRT3 gene is involved in sex determination or, to be precise, in female organ differentiation, through regulating cytokinin metabolism in V. amurensis. This study enlarged the understanding of sex determination mechanism in the genus Vitis, and the sex marker could be used as a helpful tool for sexual identification in breeding programs as well as in investigation and collection of V. amurensis germplasms.

Eineiige Zwillingsmädchen mit konkordantem Albinismus Universalis aus Ehe normalpigmentierter, aber entsprechend belasteter Vettern I. Grades in oberitalienischem Isolat

1953 ◽  
Vol 2 (3) ◽  
pp. 380-390 ◽  
Author(s):  
Ernst Hanhart
Keyword(s):  
Population Studies ◽  
Northern Italy ◽  
Frequent Occurrence ◽  
Italian Alps ◽  
Medium Frequency ◽  
The Third ◽  
Monozygous Twin ◽  
The World ◽  
Monozygous Twins ◽  
Made In

SUMMARYTwo monozygous twin girls, now 7 years old, with complete concordance of an almost total universal albinism were found in an « isolate » near Aosta in the Western Italian Alps. Their parents show normal pigmentation, and are first cousins, their common grandmothers having a brother who was the grandfather of another albinotic girl, aged 22. The parents of the latter were examined by the Dutch ophthalmogeneticist P. J. Waardenburg: while her father did not show definitely diaphane irides, her mother did. As a similar discrepancy in otherwise normal parents of albinos was stated in 2 more families (Hanhart 1952), the presence of diaphane or not diaphane irides cannot be regarded as sufficient symptom to distinguish two different biotypes of simple-recessive universal albinism. Heterogeny, though rather probable, of this character has not yet been proved in white man.In Switzerland, and Italy as well, there are considerable local accumulations of universal albinism, so that calculations on the basis of a medium frequency do not hold. The percentage of 7.9 for first cousin marriages of normal parents of albinotic children and 8% for second cousins can hardly be taken as a proof for heterogeny of the gene concerned.The observation of monozygous twins with concordant universal albinism is the first made in Europe, and the third published all over the world. It is unique on account of the near consanguinity of the normal parents of these twins. Its incidence in Northern Italy speaks in favour of a relatively frequent occurrence of this character in this country, suggested already by the author's population studies.

Spectrophotometric measurements of objective-prism spectra

1966 ◽  
Vol 24 ◽  
pp. 118-119
Author(s):  
Th. Schmidt-Kaler
Keyword(s):  
Progress Report ◽  
Two Dimensional ◽  
Objective Prism ◽  
Made In

I should like to give you a very condensed progress report on some spectrophotometric measurements of objective-prism spectra made in collaboration with H. Leicher at Bonn. The procedure used is almost completely automatic. The measurements are made with the help of a semi-automatic fully digitized registering microphotometer constructed by Hög-Hamburg. The reductions are carried out with the aid of a number of interconnected programmes written for the computer IBM 7090, beginning with the output of the photometer in the form of punched cards and ending with the printing-out of the final two-dimensional classifications.

Surface Structure in Microtomed Sections Caused by Glass and Diamond Knives

1971 ◽  
Vol 29 ◽  
pp. 456-457
Author(s):  
J. Temple Black ◽  
William G. Boldosser
Keyword(s):  
Plastic Deformation ◽  
Epoxy Resin ◽  
Carbon Coating ◽  
Surface Damage ◽  
Body Angle ◽  
Normal Manner ◽  
Bottom Side ◽  
Cutting Direction ◽  
Made In ◽  
Diamond Knife

Ultramicrotomy produces plastic deformation in the surfaces of microtomed TEM specimens which can not generally be observed unless special preparations are made. In this study, a typical biological composite of tissue (infundibular thoracic attachment) infiltrated in the normal manner with an embedding epoxy resin (Epon 812 in a 60/40 mixture) was microtomed with glass and diamond knives, both with 45 degree body angle. Sectioning was done in Portor Blum Mt-2 and Mt-1 microtomes. Sections were collected on formvar coated grids so that both the top side and the bottom side of the sections could be examined. Sections were then placed in a vacuum evaporator and self-shadowed with carbon. Some were chromium shadowed at a 30 degree angle. The sections were then examined in a Phillips 300 TEM at 60kv.Carbon coating (C) or carbon coating with chrom shadowing (C-Ch) makes in effect, single stage replicas of the surfaces of the sections and thus allows the damage in the surfaces to be observable in the TEM. Figure 1 (see key to figures) shows the bottom side of a diamond knife section, carbon self-shadowed and chrom shadowed perpendicular to the cutting direction. Very fine knife marks and surface damage can be observed.

Iron storage sites in the myocardium as revealed by cytohistochemistry

1988 ◽  
Vol 46 ◽  
pp. 394-395
Author(s):  
M. Ashraf ◽  
F. Thompson ◽  
S. Miki ◽  
P. Srivastava
Keyword(s):  
Cardiac Tissue ◽  
Coronary Perfusion ◽  
Intracellular Iron ◽  
Ether Anesthesia ◽  
Intense Staining ◽  
Iron Storage ◽  
Thin Sections ◽  
Rat Hearts ◽  
Cacodylate Buffer ◽  
Made In

Iron is believed to play an important role in the pathogenesis of ischemic injury. However, the sources of intracellular iron in myocytes are not yet defined. In this study we have attempted to localize iron at various cellular sites of the cardiac tissue with the ferrocyanide technique.Rat hearts were excised under ether anesthesia. They were fixed with coronary perfusion with 3% buffered glutaraldehyde made in 0.1 M cacodylate buffer pH 7.3. Sections, 60 μm in thickness, were cut on a vibratome and were incubated in the medium containing 500 mg of potassium ferrocyanide in 49.5 ml H2O and 0.5 ml concentrated HC1 for 30 minutes at room temperature. Following rinses in the buffer, tissues were dehydrated in ethanol and embedded in Spurr medium.The examination of thin sections revealed intense staining or reaction product in peroxisomes (Fig. 1).

Microanalysis of precipitates in a ferritic steel

1978 ◽  
Vol 36 (1) ◽  
pp. 618-619
Author(s):  
J.M. Titchmarsh
Keyword(s):  
Ferritic Steel ◽  
Particle Identification ◽  
Energy Dispersive ◽  
Objective Lens ◽  
Ferritic Steels ◽  
Replica Technique ◽  
X Ray ◽  
Large Numbers ◽  
Scanning Transmission ◽  
Made In

The advances in recent years in the microanalytical capabilities of conventional TEM's fitted with probe forming lenses allow much more detailed investigations to be made of the microstructures of complex alloys, such as ferritic steels, than have been possible previously. In particular, the identification of individual precipitate particles with dimensions of a few tens of nanometers in alloys containing high densities of several chemically and crystallographically different precipitate types is feasible. The aim of the investigation described in this paper was to establish a method which allowed individual particle identification to be made in a few seconds so that large numbers of particles could be examined in a few hours.A Philips EM400 microscope, fitted with the scanning transmission (STEM) objective lens pole-pieces and an EDAX energy dispersive X-ray analyser, was used at 120 kV with a thermal W hairpin filament. The precipitates examined were extracted using a standard C replica technique from specimens of a 2¼Cr-lMo ferritic steel in a quenched and tempered condition.

AEM investigation of ZrO2 solid solution formation in ZrO2/mullite composites

1984 ◽  
Vol 42 ◽  
pp. 408-409
Author(s):  
T. R. Dinger
Keyword(s):  
Solid Solution ◽  
Ion Beam ◽  
Analytical Electron Microscopy ◽  
Transformation Toughening ◽  
Solid Solution Formation ◽  
Solution Formation ◽  
Propagating Crack ◽  
Analytical Electron ◽  
Microcrack Toughening ◽  
Made In

Zirconia (ZrO2) is often added to ceramic compacts to increase their toughness. The mechanisms by which this toughness increase occurs are generally accepted to be those of transformation toughening and microcracking. The mechanism of transformation toughening is based on the presence of metastable tetragonal ZrO2 which transforms to the monoclinic allotrope when stressed by a propagating crack. The decrease in volume which accompanies this transformation effectively relieves the applied stress at the crack tip and toughens the material; microcrack toughening arises from the deflection of a propagating crack around sharply angular inclusions.These mechanisms, however, do not explain the toughness increases associated with the class of composites investigated here. Analytical electron microscopy (AEM) has been used to determine whether solid solution effects could be the cause of this increased toughness. Specimens of a mullite (3Al2O3·2SiO2) + 15 vol. % ZrO2 were prepared by the usual technique of mechanical thinning followed by ion beam milling. All observations were made in a Philips EM400 TEM/STEM microscope fitted with EDXS and EELS spectrometers.

A study of interface sliding at F.C.C.-B.C.C. boundaries in a Cu-Zn alloy

1978 ◽  
Vol 36 (1) ◽  
pp. 422-423
Author(s):  
F. Monchoux ◽  
A. Rocher ◽  
J.L. Martin
Keyword(s):  
Face Centered Cubic ◽  
Creep Tests ◽  
High Voltage Electron Microscopy ◽  
Diffusion Treatment ◽  
Voltage Electron ◽  
The Face ◽  
Face Centered ◽  
Interface Sliding ◽  
Zn Alloy ◽  
Made In

Interphase sliding is an important phenomenon of high temperature plasticity. In order to study the microstructural changes associated with it, as well as its influence on the strain rate dependence on stress and temperature, plane boundaries were obtained by welding together two polycrystals of Cu-Zn alloys having the face centered cubic and body centered cubic structures respectively following the procedure described in (1). These specimens were then deformed in shear along the interface on a creep machine (2) at the same temperature as that of the diffusion treatment so as to avoid any precipitation. The present paper reports observations by conventional and high voltage electron microscopy of the microstructure of both phases, in the vicinity of the phase boundary, after different creep tests corresponding to various deformation conditions.Foils were cut by spark machining out of the bulk samples, 0.2 mm thick. They were then electropolished down to 0.1 mm, after which a hole with thin edges was made in an area including the boundary

Searching for the causes why some of the paintings by Mihaly Munkacsy are darkening

1990 ◽  
Vol 48 (2) ◽  
pp. 204-205
Author(s):  
Imre Pozsgai ◽  
Klara Erdöhalmi-Torok
Keyword(s):  
Electron Beam ◽  
Cross Section ◽  
Polyester Resin ◽  
National Gallery ◽  
X Ray ◽  
Art Historians ◽  
Made In ◽  
Grinding And Polishing

The paintings by the great Hungarian master Mihaly Munkacsy (1844-1900) made in an 8-9 years period of his activity are deteriorating. The most conspicuous sign of the deterioration is an intensive darkening. We have made an attempt by electron beam microanalysis to clarify the causes of the darkening. The importance of a study like this is increased by the fact that a similar darkening can be observed on the paintings by Munkacsy’s contemporaries e.g Courbet and Makart. A thick brown mass the so called bitumen used by Munkacsy for grounding and also as a paint is believed by the art historians to cause the darkening.For this study, paint specimens were taken from the following paintings: “Studio”, “Farewell” and the “Portrait of the Master’s Wife”, all of them are the property of the Hungarian National Gallery. The paint samples were embedded in a polyester resin “Poly-Pol PS-230” and after grinding and polishing their cross section was used for x-ray mapping.

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