Genetic variation in Eruca vesicaria (L.) Cav.

2007 ◽  
Vol 5 (03) ◽  
pp. 142-153 ◽  
Author(s):  
S. I. Warwick ◽  
R. K. Gugel ◽  
C. Gómez-Campo ◽  
T. James
Keyword(s):  
Field Trial ◽  
Seed Quality ◽  
Quality Data ◽  
Oilseed Crop ◽  
Aflp Data ◽  
Cruciferous Vegetable ◽  
Western Africa ◽  
Length Polymorphisms ◽  
Western Asia ◽  
Agronomic Potential

Eruca vesicariasubsp.sativa(syn.E. sativa) is a cruciferous vegetable and oilseed crop that is high in erucic acid. It occurs throughout the Mediterranean region and western Asia, and has been naturalized elsewhere as a crop/weed escape. It is closely related to subsp.vesicariaand subsp.pinnatifida, which are endemic to Spain and north-western Africa, respectively. This study evaluated patterns and levels of diversity in the three subspecies based on 234 amplified fragment length polymorphisms (AFLP), and evaluated agronomic and seed quality data in a field trial in western Canada. AFLP data revealed three main clusters: ‘Sativa’ (33 accessions of subsp.sativa), ‘Vesicaria’ (nine accessions of subsp.vesicaria) and a ‘Pinnatifida’ cluster (one accession of subsp.pinnatifidaand three Moroccan accessions of subsp.sativa). The Sativa cluster separated into Mediterranean and Asian groups, likely reflecting differences in origin (wild versus cultivated) or primary usage, vegetable versus seed oil. The origin of the introduced Mexican population was confirmed as subsp.sativa. The highest levels of diversity were found in the Sativa cluster (88% AFLP polymorphisms) and the least in the Vesicaria (56%) and Pinnatifida (39%) clusters. Extensive variation was observed among the 159 subsp.sativaaccessions evaluated in the field trial, and overall findings indicated a favourable agronomic potential.

Genetic diversity of Echinacea species based upon amplified fragment length polymorphism markers

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 102-111 ◽  
Author(s):  
D -H Kim ◽  
D Heber ◽  
D W Still
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Fragment Length ◽  
Echinacea Purpurea ◽  
Morphological Characters ◽  
Pairwise Distance ◽  
Neighbor Joining ◽  
Aflp Data ◽  
Phenetic Relationships ◽  
Length Polymorphisms

The taxonomy of Echinacea is based on morphological characters and has varied depending on the monographer. The genus consists of either nine species and four varieties or four species and eight varieties. We have used amplified fragment length polymorphisms (AFLP) to assess genetic diversity and phenetic relationships among nine species and three varieties of Echinacea (sensu McGregor). A total of 1086 fragments, of which approximately 90% were polymorphic among Echinacea taxa, were generated from six primer combinations. Nei and Li's genetic distance coefficient and the neighbor-joining algorithm were employed to construct a phenetic tree. Genetic distance results indicate that all Echinacea species are closely related, and the average pairwise distance between populations was approximately three times the intrapopulation distances. The topology of the neighbor-joining tree strongly supports two major clades, one containing Echinacea purpurea, Echinacea sanguinea, and Echinacea simulata and the other containing the remainder of the Echinacea taxa (sensu McGregor). The species composition within the clades differs between our AFLP data and the morphometric treatment offered by Binns and colleagues. We also discuss the suitability of AFLP in determining phylogenetic relationships.Key words: Echinacea, AFLP, genetic distance, phylogeny.

scholarly journals Effects of Genotype, Direct Sowing and Plant Spacing on Field Performance of Jatropha curcas L.

Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 465
Author(s):  
Zafitsara Tantely Andrianirina ◽  
Matthias Martin ◽  
Euloge Dongmeza ◽  
Elisa Senger
Keyword(s):  
Seed Yield ◽  
Jatropha Curcas ◽  
Seed Quality ◽  
Field Performance ◽  
Correct Choice ◽  
Biodiesel Production ◽  
Oilseed Crop ◽  
Jatropha Curcas L ◽  
Seed Yields ◽  
Direct Sowing

The tropical multiuse tree Jatropha curcas L. (jatropha) is highly promoted as oilseed crop for biodiesel production and for climate change mitigation, but cultivation practices require further research. The objectives of this study were to assess the effects of varying plant spacings (2.0 m × 4 m compared to 1.5 m × 4 m), crop establishment methods (raising plantlets in a nursery prior to planting to the field compared to direct sowing) and genotypes on seed yield, seed quality and plant height, recorded at a dry-subhumid location in Madagascar (Ihosy) and at a humid location in Cameroon (Batchenga). Averaged across treatment variants and genotypes, seed yield and seed oil content were higher at the dry-subhumid site and in particular the narrower spacing reached higher seed yields per unit area than the wider spacing. At the humid site, plant growth was characterized by strong accumulation of biomass. The establishment method tested at the dry-subhumid site showed no significant differences in the recorded parameters. Our results encourage to re-think common practices in jatropha cultivation and underpin the importance of the correct choice of location, genotype and agronomic practices considering the interactions between all factors.

Evaluating the Taxonomic Status of the Globally Rare Carex roanensis and Allied Species Using Morphology and Amplified Fragment Length Polymorphisms

2008 ◽  
Vol 33 (3) ◽  
pp. 525-535 ◽  
Author(s):  
Tyler W. Smith ◽  
Marcia J. Waterway
Keyword(s):  
Fragment Length ◽  
Taxonomic Status ◽  
Principal Coordinate Analysis ◽  
Species Level ◽  
Morphological Data ◽  
Aflp Data ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
The Common ◽  
Geographic Regions

We used a combination of amplified fragment length polymorphisms (AFLP) and morphological data from 272 individuals from 59 populations to investigate the species-level taxonomy of Carex roanensis and allied species. There were two taxonomic problems in this group: identifying the appropriate taxonomic status for Carex roanensis, and clarifying the distinctions (if any) between C. virescens and C. swanii. Principal coordinate analysis of the morphological data suggested four entities corresponding to C. aestivalis, C. roanensis, C. swanii, and C. virescens, but clear discrimination was not possible. In contrast, the AFLP data showed marked discontinuities among these four species, placing even morphological intermediates into one of four groups. Analysis of molecular variance revealed significant population differentiation within each species, but only C. virescens had any detectable differentiation between geographic regions. This study confirms the species-level distinction between the common and widespread taxa C. swanii and C. virescens, as well as that of the globally rare Appalachian endemic C. roanensis.

AFLP-based molecular characterization of Brassica rapa and diversity in Canadian spring turnip rape cultivars

2008 ◽  
Vol 6 (1) ◽  
pp. 11-21 ◽  
Author(s):  
S. I. Warwick ◽  
T. James ◽  
K. C. Falk
Keyword(s):  
Genetic Diversity ◽  
Brassica Rapa ◽  
Genetic Relationships ◽  
Aflp Data ◽  
Distance Information ◽  
Breeding Lines ◽  
Cultivar Development ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Aa Genome

Information on genetic diversity and genetic relationships among taxa of Brassica rapa (n = 10, AA genome) is currently limited. Grown for oil, vegetable and fodder use in Europe and Asia, previous studies have indicated western and eastern groups corresponding to independent centres of origin. This study evaluated patterns and levels of genetic diversity in 93 accessions [includes 25 Agriculture and Agri-Food Canada (AAFC) breeding lines (BL)] of B. rapa based on 307 amplified fragment length polymorphisms (AFLP), testing subspecific separateness and the affiliation of four previously unassigned AA genome species (B. perviridis, B. purpuraria, B. ruvo and B. septiceps). AFLP data revealed three main clusters (I, II, III) corresponding to European (I), Indian (III), and a mixed Asian/European/Indian (II) purported origins of the taxa, with several subclusters observed in I and II. Mean AFLP polymorphism levels for Asian, European, Indian and AAFC-BL accessions were 79, 74, 66 and 62%, respectively. Few of the subspecies formed unique clusters and some, particularly subspecies chinensis and pekinensis, were assigned to several clusters. AFLP-based genetic distance information can be used by breeders to select diverse genotypes for cultivar development and fingerprinting of genotypes/cultivars. For example, a single AFLP primer pair was sufficient to uniquely identify all breeding lines in the AAFC B. rapa breeding programme.

scholarly journals The response of Stecklings Age and Plant Spacing on Carrot Seed Quality and Production

2020 ◽  
Vol 3 (2) ◽  
pp. 71
Author(s):  
Fatiani Manik ◽  
Rasiska Tarigan ◽  
Susilawati Barus
Keyword(s):  
Seed Production ◽  
Field Trial ◽  
Seed Quality ◽  
Block Design ◽  
Plant Spacing ◽  
Randomized Complete Block Design ◽  
Two Factors ◽  
Carrot Seed

The quality of seed needs to be considered in carrot seed production to maintain its productivity. The research aims to find out the response of stecklings age and plant spacing on carrot seed quality and production. This research was held on July 2018 – April 2019 in Berastagi field trial (1340 m MSL). The experiment used Randomized Complete Block Design (RCBD) with two factors, i.e., stecklings age (2 and 3 months) and plant spacing (25, 50, 75, and 100 cm), replicated four times (32 experiment units), 10 plants from each experiment units were taken as samples. The result showed that stecklings age and plant spacing were affected to plant vegetative and generative stages. The combination of 2-month stecklings and 50 cm plant spacing increased the number of secondary umble, if compared to cultivation, which was generally used by the farmer (3-month stecklings and 25 cm plant spacing). The best quality of carrot seed was obtained from the combination of 3-month stecklings and 50 cm plant spacing.

scholarly journals Development of a methodology for allocating seed production zones based on established types of seed yield and soil-climatic zoning

2021 ◽  
pp. 85-98
Author(s):  
Y. V. Plugatar' ◽  
N. M. Makrushin ◽  
E. M. Makrushina ◽  
T. S. Naumenko
Keyword(s):  
Seed Production ◽  
Seed Yield ◽  
Seed Quality ◽  
Climatic Factors ◽  
Quality Data ◽  
High Yield ◽  
Type I ◽  
Agricultural Technologies ◽  
Seed Material ◽  
Quality Type

The method of allocation of zones of seed production of agricultural plants depending on soil and climatic factors is developed on the basis of the established four types of seed yield: type I - high yield, high seed quality; type II - high yield, low seed quality; type III - low yield, high seed quality; IV - low yield, low seed quality. Data on crop types were plotted on a contour map of the studied administrative subject (region, territory, republic) with the designated boundaries of districts and soil-climatic zones, as a result, 4 possible zones of seed production were identified: 1- the zone of optimal seed production, in which the seed farms form the I type of crop; 2 - the zone of risky seed production (II type of crop); 3 - the zone of permissible seed production (III type of crop); 4 - the zone of unacceptable seed production (IV type of crop). The network of seed farms should be organized primarily in the areas of optimal seed production. When organizing the production of seed material in the zones of permissible seed production, it is necessary to increase the yield by improving agricultural technologies. In the zones of risky (II type of crop) and unacceptable (IV type of crop) seed production, it is not advisable to produce seed material.

scholarly journals Length polymorphisms of simple sequence repeat (SSR) markers in assisted selection for lipoxygenase-free in soybean

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
VALERIA CARPENTIERI-PIPOLO ◽  
Thales Pereira Barreto ◽  
Daiana Alves Silva ◽  
Ricardo Vilela Abdelnoor ◽  
Silvana Rockenbach Marin ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Null Allele ◽  
Seed Quality ◽  
Soybean Seed ◽  
Hybrid Population ◽  
Human Consumption ◽  
F1 Hybrid ◽  
Hybrid Plants ◽  
Length Polymorphisms ◽  
Glycine Max L

Beany flavor of soybean (Glycine max (L.) Merr.) is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases (LOX1, LOX2 and LOX3) present in mature seeds. The unpleasant flavor restricts human consumption of soybean products. This problem could be solved through genetic elimination of alleles that code these enzymes. Parental cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus, encoding Lox1, Lox2 and Lox3 free. The SSR marker Satt212 confirmed the presence of the homozygous null-allele Lx3 in the cultivar BRS 213, which were used for hybridization with BR 36. Heterozygote F1 hybrid plants and homozygous Lx3 lines in F2 segregating populations were successfully identified.  The SSR markers Sat090 and Sat417 was the most effective diagnostic marker among the all SSR markers tested. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3,00 and 2,77 cM, respectively. The presence of Lx2 null allele in The F2 segregating populations between BRS 213 and BRS 155 were successfully identified with a selection efficiency of 98% and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality.

scholarly journals Does Primula intricata Gren. et Godr. Merit Species Rank? A Taxonomic Revision Based on nrDNA, cpDNA and AFLP Data

2011 ◽  
Vol 39 (1) ◽  
pp. 24
Author(s):  
Dana ŞUTEU ◽  
Mihai PUSCAS ◽  
Ioan BĂCILĂ ◽  
Ana COSTE ◽  
Liviu FILIPAS ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Taxonomic Status ◽  
Taxonomic Revision ◽  
Distinct Species ◽  
Nuclear Ribosomal Dna ◽  
Valid Species ◽  
Aflp Data ◽  
Genetic Studies ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms

Primula intricata Gren. et Godr. has an unclear taxonomic status: it was originally described as a distinct species but subsequently was considered a subspecies (Primula elatior subsp. intricata) or even a variety (P. elatior var. intricata ) of Primula elatior (L.) Hill. No prior genetic studies were performed on this group of Primulaceae, therefore we considered useful to investigate taxonomies boundaries within the P. elatior-intricata group. We explored genetic differences between Primula intricata and Primula elatior group by applying three different types of molecular markers: nuclear ribosomal DNA (ITS1), chloroplast DNA (spacer trnH-psbA and intron trnL) and Amplified Fragment Length Polymorphisms (AFLP). We found a solid differentiation between P. intricata and P. elatior group, differentiation that was confirmed by all the employed molecular markers. This finding enabled us to propose a valid species rank for Primula intricata, as a separate taxon from the P. elatior group.

The effect of cultivar and applied nitrogen on the performance of Camelina sativa L. in the Maritime Provinces of Canada

2008 ◽  
Vol 88 (1) ◽  
pp. 111-119 ◽  
Author(s):  
S. D. Urbaniak ◽  
C. D. Caldwell ◽  
V. D. Zheljazkov ◽  
R. Lada ◽  
L. Luan
Keyword(s):  
Plant Height ◽  
Seed Yield ◽  
Oil Content ◽  
Seed Protein ◽  
Seed Quality ◽  
Camelina Sativa ◽  
Oilseed Crop ◽  
Maritime Provinces ◽  
N Application ◽  
Total N

Worldwide, oilseed demand is steadily expanding. This study assessed the novel oilseed crop Camelina sativa L. in the Maritime Provinces of Canada. Evaluations of cultivar and applied N were performed at Truro, NS, Harrington, PEI, and Hartland, NB, in 2005 and 2006. The results show that the selection of cultivar is an important determinant for the potential success or failure of C. sativa production. Differences in plant stand, plant height, seed yield, oil content and fatty acid profile were found among the cultivars. The cultivar Calena provided the most consistent performance in terms of plant establishment, yield and oil content across all year-sites. Plant height, seed yield, oil content, total plant N and seed protein all responded to applied N rate. Applied N increased the seed yield of C. sativa, but the increase was not significant when the application rates were over 60 kg N ha-1 in NS or 80 kg N ha-1 in PEI. Plant height, total N content in plant tissue and seed protein content increased with increased N application, while oil content decreased. With the exception of erucic acid, all the main fatty acids in C. sativa oil responded to applied N levels or the interaction of N levels and year. Regression analysis showed that the contents of oleic acid and eicosenoic acid decreased with increased N application. The response of linolenic acid to N application was not consistent at each year-site. Results of this study provide convincing evidence of the agronomic suitability of C. sativa to the Maritime Provinces of Canada. Key words: Camelina sativa, cultivar, nitrogen, yield, seed quality

Somaclonal variation in seed germination of dallisgrass biotypes

2005 ◽  
Vol 3 (3) ◽  
pp. 414-420 ◽  
Author(s):  
W.D. Pitman ◽  
S.S. Croughan ◽  
J.L. Nash ◽  
B.C. Venuto
Keyword(s):  
Tissue Culture ◽  
Seed Germination ◽  
Somaclonal Variation ◽  
Seed Quality ◽  
Germination Percentage ◽  
Forage Production ◽  
Paspalum Dilatatum ◽  
Pasture Grass ◽  
Plant Persistence ◽  
Agronomic Potential

Dallisgrass (Paspalum dilatatum Poir.) is an important pasture grass on moist fertile sites in warm climates, however, it is limited in use by periodic low seed quality and resulting erratic availability and establishment difficulties. Due to apomictic reproduction, common pentaploid dallisgrass has not been responsive to traditional plant improvement approaches. Recent research has shown potential for some improvement in forage production and plant persistence with apomictic hexaploid varieties of dallisgrass. Tissue-culture regeneration has been reported to produce somaclonal variation in seed germination of common pentaploid dallisgrass and has recently resulted in availability of potentially useful regenerants of hexaploid dallisgrass. Seed of selected plants from both field and greenhouse environments was evaluated in replicated germination trials to determine whether hexaploid tissue-culture regenerants responded similarly to regenerants from common dallisgrass and to determine whether regenerants expressing potential agronomic usefulness as indicated by preliminary observations differed in germination. The eight germination trials included 299 regenerants with 57 of these being duplicate entries among trials. Germination did not differ among sources of regenerants. Within sources germination differed among regenerants with a few individuals exceeding germination percentage of parent lines in one evaluation of greenhouse-produced seed. Individual regenerants with agronomic potential and high percentage seed germination were identified. The results demonstrate that tissue-culture regeneration can be used to produce improved germination in agronomically useful genotypes of apomictic dallisgrass and suggest that such potential may exist for other recalcitrant plant species.

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