scholarly journals Processing Cochlea For Paraffin Sectioning And SEM

1998 ◽  
Vol 6 (5) ◽  
pp. 28-29
Author(s):  
Donna C. Montague
Keyword(s):  
Guinea Pig ◽  
Formic Acid ◽  
Room Temperature ◽  
Test Tube ◽  
List Type ◽  
Distilled Water ◽  
Type Number ◽  
Guinea Pig Cochlea ◽  
Specimen Volume ◽  
Gentle Agitation

We have successfully sectioned decalcified paraffin embedded “inner ears” from mice, rats and humans. We have also prepared guinea pig cochlea for SEM, So, briefly:Paraffin:1)Immediately after dissection, place the cochlea in 10% neutral buffered formalin (NSF) and let fix with gentle agitation 24 hours per 2 mm thickness at room temperature.2)Decalcify by immersing in 5% formic acid at least 4X specimen volume.3)Gently agitate at room temperature and change at least once per 24 hours. Check for completeness of decalcification by decanting 4 mLs of “spent” formic acid into a clean, clear test tube, Add 1 mL of 5% ammonium oxaiate in distilled water. Let stand undisturbed for 15 to 20 minutes, White precipitate of calcium oxaiate indicates that the sample is not completely decalcified, Change formic acid at least once per 24 hours until the spent formic tests clear.

scholarly journals Getting Epoxy Semi-Thin Sections to Stick to Glass Slides

2007 ◽  
Vol 15 (4) ◽  
pp. 52-52
Author(s):  
Gilbert (Gib) Ahlstrand
Keyword(s):  
Toluidine Blue ◽  
Room Temperature ◽  
Water Drop ◽  
List Type ◽  
Distilled Water ◽  
Type Number ◽  
Blow Down ◽  
Simple Method ◽  
Thin Sections ◽  
Glass Slides

Semi-thin sections don't always want to stick to glass slides, but subbing slides is usually not needed. For sections about 2 μm thick and no larger than 4 mm on a slide, this simple method works well for me:1)Clean 1x3 inch glass slides with an ethanol rinse, then air dry at room temperature or blow down with a hair dryer.2)Collect sections on a drop or two of distilled water on the slide, transferred there from the microtome with a clean fine tipped artists brush. Collect about 8-12 sections per drop.3)Warm the slide beneath the water drop from below using an alcohol lamp, fairly hot, but not to boil, of course. After drying by heating the sections stick quite well.4)Stain, usually with 0.2 μm filtered toluidine blue, again heating but gently this time, for about a minute, until stain “develops” the section.5)Rinse that stain off with distilled water from a squirt bottle, even directing the spray right onto the sections to get rid of any precipitate. Dry again gently with flame.The heating is the trick. There should not be any need for subbing or otherwise treating slides other than cleaning them.

Hydration Chamber for Jeolco 200 Kv Microscope

1970 ◽  
Vol 28 ◽  
pp. 542-543
Author(s):  
V. R. Matricardi ◽  
G. G. Hausner ◽  
D. F. Parsons
Keyword(s):  
Electron Microscope ◽  
Water Vapor ◽  
Vapor Pressure ◽  
Pressure Gradient ◽  
Room Temperature ◽  
List Type ◽  
Type Number ◽  
Equilibrium Vapor Pressure

In order to observe room temperature hydrated specimens in an electron microscope, the following conditions should be satisfied: The specimen should be surrounded by water vapor as close as possible to the equilibrium vapor pressure corresponding to the temperature of the specimen.The specimen grid should be inserted, focused and photo graphed in the shortest possible time in order to minimize dehydration.The full area of the specimen grid should be visible in order to minimize the number of changes of specimen required.There should be no pressure gradient across the grid so that specimens can be straddled across holes.Leakage of water vapor to the column should be minimized.

A new method for searching intracellular stereo structures in plant tissues

1990 ◽  
Vol 48 (3) ◽  
pp. 720-721
Author(s):  
Yaqin Yao ◽  
Xuanli Jiang ◽  
Dongwei Hu
Keyword(s):  
Room Temperature ◽  
Plant Tissues ◽  
Specimen Preparation ◽  
List Type ◽  
Type Number ◽  
Cell Organelles ◽  
Mesophyll Cells ◽  
Thin Sections ◽  
Wheat Leaves ◽  
Ethanol Series

The procedure for specimen preparation consist of the following. 1.fixing the plant tissues routinely;2.washing the blocks thoroughly in PBS buffer;3.immersing the blocks in a PEG (polyethylene glycol 4000) series (50, 70, 90, 100%) and embedding in pure PEG at 50°C, and cooling slowly to room temperature;4.cutting into thin sections 5-10 nm thick by LKB-V microtome;5.washing the sections a few times in water, dehydrating in a graded ethanol series, and drying in a critical-point dryer using liquid CO2;6.sputter-coating with gold palladium; and7.investigating in a Hitachi S-450 scanning electron microscope.By this method, the distinct, uninjured intracellular stereo structures can be conveniently found without Joss of cell organelles. Moreover, there are advantages to studying their stereo relationships between various components of cells. Using wheat leaves, one can find chloroplasts, mitochondria, nuclei, nucleoli, and parts of the cytoskeleton system in the mesophyll cells and their sizes and distributions directly.

En bloc staining available for stereoscopic observation of epoxy resin Quetol 651-embedded thick sections under a 300 kV TEM

1990 ◽  
Vol 48 (3) ◽  
pp. 740-741
Author(s):  
Tsuyuka Kushida ◽  
Haruyuki Iijima ◽  
Hiroshi Kushida ◽  
Chusei Tsuruta
Keyword(s):  
Epoxy Resin ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
List Type ◽  
Distilled Water ◽  
Thick Section ◽  
En Bloc ◽  
Fixed Tissue ◽  
Stereoscopic Observation

A staining method has been devised for easy en bloc staining for stereoscopic observation of epoxy resin Quetol 651-embedded thick sections under a 300 kV transmission microscope (TEM). In order to enhance staining properties in thick section, osmium tetroxide-fixed tissue blocks are stained only en bloc, since the images of both sides in thick section give high contrast and the image of an intermediate layer shows low contrast by double staining.This method uses carbohydrazide (Polysciences, Inc., U.S.A.) as osmium bridging agent, and both osmium tetroxide and uranyl acetate as electron staining agents.The following procedure is suitable for en bloc staining. 1.Fix small tissue blocks in 2% cacodylate-buffered osmium tetroxide (pH 7.4) for 3 hours at 4°C.2.Wash well in buffer for 1 hour.3.Transfer in 1% aqueous carbohydrazide for 2 hours at room temperature.4.Wash well in distilled water for 1 hour.5.Stain in 1% aqueous osmium tetroxide for 2 hours at room temperature.6.Wash well in distilled water for 1 hour.7.Dehydrate in 50% alcohol for 1 hour.8.Stain in a 2.5% solution of uranyl acetate in 50% alcohol for 3 hours at room temperature.9.Wash in 50% alcohol for 1 hour.10.Dehydrate with 60%, 70%, 80%, 90% and 100% (2 changes) alcohols for 30 minutes each.11.Embed in a mixture of Quetol 651 (Nissin EM Co., Ltd., Japan), nonenyl succinic anhydride, methyl nadic anhydride and DMP-30 according to the method of Kushida et al.

Systematics of Silicide Formation by High Dose Miplantation of Transition Metals into Si

1986 ◽  
Vol 74 ◽  
Author(s):  
F. Namavar ◽  
F. H. Sanchez ◽  
J. I. Budnick ◽  
A. H. Fasihuddin ◽  
H. C. Hayden
Keyword(s):  
Room Temperature ◽  
Hexagonal Structure ◽  
High Dose ◽  
List Type ◽  
Type Number ◽  
Silicide Formation ◽  
Metal Thin Films ◽  
X Ray ◽  
Cubic Structure ◽  
Fe Implantation

AbstractWe have systematically studied the formation of transition-metal thin films by high dose (up to 1018 ions/cm2) implantation of Ti, V, Cr, Mn, Fe, Co, Ni and Nb at room temperature and 350°C into Si <100>.For implantation at 350°C, our results, as obtained by Rutherford backscattering, X-ray diffractometry and Read Camera measurements, indicate that one can categorize these metals into two groups: 1.a chromium group which includes V, Cr, Nb, Ti and Mn. Metals V, Cr and Nb form compounds (VSi2, CrSi2. NbSi2) with a hexagonal structure of the CrSi2 type whereas Ti and Mn both form compounds (Ti5Si3, Mn5Si3) with a hexagonal structure of the Mn5Si2 type.2.an iron group which includes Fe, Co and Ni. These metals form compounds (FeSi, CoSi, NiSi) with a cubic structure of the FeSi type.In this paper the experimental results for Cr and Fe implantation at room temperature and 350°C will be discussed.

Solar Corona Investigation by the Coronal Line Photometer at Lomnický Peak

1994 ◽  
Vol 144 ◽  
pp. 431-434
Author(s):  
M. Minarovjech ◽  
M. Rybanský
Keyword(s):  
Solar Corona ◽  
Time Resolution ◽  
Active Regions ◽  
High Time ◽  
High Time Resolution ◽  
List Type ◽  
Type Number ◽  
Coronal Line ◽  
Global Cycle

AbstractThis paper deals with a possibility to use the ground-based method of observation in order to solve basic problems connected with the solar corona research. Namely:1.heating of the solar corona2.course of the global cycle in the corona3.rotation of the solar corona and development of active regions.There is stressed a possibility of high-time resolution of the coronal line photometer at Lomnický Peak coronal station, and use of the latter to obtain crucial observations.

The LDE-Type Flare Occurrence (1969-1993)

1994 ◽  
Vol 144 ◽  
pp. 279-282
Author(s):  
A. Antalová
Keyword(s):  
Time Series ◽  
Fourier Analysis ◽  
Sunspot Cycle ◽  
List Type ◽  
Type Number ◽  
Solar Cycles ◽  
Type Iv ◽  
Long Duration ◽  
Cycle Maximum ◽  
Flare Index

AbstractThe occurrence of LDE-type flares in the last three cycles has been investigated. The Fourier analysis spectrum was calculated for the time series of the LDE-type flare occurrence during the 20-th, the 21-st and the rising part of the 22-nd cycle. LDE-type flares (Long Duration Events in SXR) are associated with the interplanetary protons (SEP and STIP as well), energized coronal archs and radio type IV emission. Generally, in all the cycles considered, LDE-type flares mainly originated during a 6-year interval of the respective cycle (2 years before and 4 years after the sunspot cycle maximum). The following significant periodicities were found:• in the 20-th cycle: 1.4, 2.1, 2.9, 4.0, 10.7 and 54.2 of month,• in the 21-st cycle: 1.2, 1.6, 2.8, 4.9, 7.8 and 44.5 of month,• in the 22-nd cycle, till March 1992: 1.4, 1.8, 2.4, 7.2, 8.7, 11.8 and 29.1 of month,• in all interval (1969-1992):a)the longer periodicities: 232.1, 121.1 (the dominant at 10.1 of year), 80.7, 61.9 and 25.6 of month,b)the shorter periodicities: 4.7, 5.0, 6.8, 7.9, 9.1, 15.8 and 20.4 of month.Fourier analysis of the LDE-type flare index (FI) yields significant peaks at 2.3 - 2.9 months and 4.2 - 4.9 months. These short periodicities correspond remarkably in the all three last solar cycles. The larger periodicities are different in respective cycles.

Discussion following the presentation by F. Deubner

1977 ◽  
Vol 36 ◽  
pp. 69-74
Keyword(s):  
List Type ◽  
The Sun ◽  
Type Number ◽  
Harmonic Motion ◽  
Locally Excited ◽  
Long Period ◽  
Coherent Wave ◽  
Short Period ◽  
Global Modes

The discussion was separated into 3 different topics according to the separation made by the reviewer between the different periods of waves observed in the sun :1) global modes (long period oscillations) with predominantly radial harmonic motion.2) modes with large coherent - wave systems but not necessarily global excitation (300 s oscillation).3) locally excited - short period waves.

Prominences and Solar Activity

1979 ◽  
Vol 44 ◽  
pp. 357-372
Author(s):  
Z. Švestka
Keyword(s):  
Solar Activity ◽  
Solar Cycle ◽  
List Type ◽  
Type Number ◽  
Flare Loops ◽  
Filament Activation

The following subjects were discussed:(1)Filament activation(2)Post-flare loops.(3)Surges and sprays.(4)Coronal transients.(5)Disk vs. limb observations.(6)Solar cycle variations of prominence occurrence.(7)Active prominences patrol service.Of all these items, (1) and (2) were discussed in most detail and we also pay most attention to them in this report. Items (3) and (4) did not bring anything new when compared with the earlier invited presentations given by RUST and ZIRIN and therefore, we omit them.

A New System For Optomanual Semiautomatic Quantitative Image Analysis

1977 ◽  
Vol 35 ◽  
pp. 210-211
Author(s):  
H.P. Rohr
Keyword(s):  
Image Analysis ◽  
Volume Density ◽  
List Type ◽  
Distribution Analysis ◽  
Type Number ◽  
Point Counting ◽  
Human Eye ◽  
Quantitative Image ◽  
Point Density ◽  
Electronic Counting

Today, in image analysis the broadest possible rationalization and economization have become desirable. Basically, there are two approaches for image analysis: The image analysis through the so-called scanning methods which are usually performed without the human eye and the systems of optical semiautomatic analysis completely relying on the human eye.The new MOP AM 01 opto-manual system (fig.) represents one of the very promising approaches in this field. The instrument consists of an electronic counting and storing unit, which incorporates a microprocessor and a keyboard for choice of measuring parameters, well designed for easy use.Using the MOP AM 01 there are three possibilities of image analysis:the manual point counting,the opto-manual point counting andthe measurement of absolute areas and/or length (size distribution analysis included).To determine a point density for the calculation of the corresponding volume density the intercepts lying within the structure are scanned with the light pen.

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