scholarly journals “IF YOU DON’T KNOW ME BY NOW…”

Author(s):  
Ellen M. Whitehead ◽  
Allan Farrell ◽  
Jenifer L. Bratter

ABSTRACT The racial composition of couples is a salient indicator of race’s impact on mate selection, but how well do those in intimate partnerships know the racial identities of their partners? While prior research has revealed that an individual’s race may be perceived differently than how they identify, most of what is known comes from brief interactions, with less information on established relationships. This study examines whether discrepancies in the reports of a person’s race or ethnicity can be identified even within intimate relationships, as well as which relational, social, and attitudinal factors are predictive of divergent or concordant reports. We draw on the Fragile Families and Child Wellbeing Study (n=3467), a U.S.-based dataset that uniquely provides both the father’s self-reported race and Hispanic origin and the mother’s report of the father’s race and ethnicity. We compare reports of the father’s race/Hispanic origin from both parents to assess the extent of mismatch, and we distinguish between whether mothers view the father’s race as similar to or different from her own. We find roughly 14% of mothers provide a race and Hispanic origin that is inconsistent with the father’s report, with a large share reflecting differences in the self-identified and perceived race of fathers who are reported as Hispanic. Among mismatched reports, mothers are more likely to report a race/ethnicity for the father that matches her own, depressing the number reporting interracial unions. Perceptions of racial homogamy are especially likely when mothers view racial sameness as important to marriage. Further, mismatches are more common in the midst of weak relational ties (i.e. non-marital relationships) and are less common when both parents are college-educated. These findings reveal that intimate unions are a site where race is socially constructed and provide insight into how norms of endogamy manifest within formed relationships.

2021 ◽  
Vol 2 (1) ◽  
pp. 26-38
Author(s):  
Elizabeth S. Parks

Hybridity, when related to identity research, points to a blending of difference in such a way that new performances of identity may appear in various social interactions. Exploring hybridity can create new perceptions of social life and reveal the complex ways that people’s diverse relationships and life stages construct people’s identities. In this critical autoethnography, I consider how disability and racial identities intersect. More specifically, I relate how my narrative experience with the disabling symptoms of Graves’ Disease impacted the ascription of Asian racial identity based on the reading of the physicality of my eyes. Grounded in these personal narratives, I theorize about ways that hybridity can cross boundaries of categorical difference in the ways that it is socially constructed, fluid, and changing. Some changes are expected, as age transforms all of us; others are unexpected, as the body and mind are surprised by illness and rapid physical changes impact avowed and ascribed identities. I offer this autoethnography of these intersecting spaces as one performance of evolving identity work that impacted my own and others’ imagination of race and disability. I hope to create new insight into how our social worlds are constructed (and privileged ideologies promoted) in everyday life.


Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 738
Author(s):  
Nicola Rossi ◽  
Mario Bačić ◽  
Meho Saša Kovačević ◽  
Lovorka Librić

The design code Eurocode 7 relies on semi-probabilistic calculation procedures, through utilization of the soil parameters obtained by in situ and laboratory tests, or by the means of transformation models. To reach a prescribed safety margin, the inherent soil parameter variability is accounted for through the application of partial factors to either soil parameters directly or to the resistance. However, considering several sources of geotechnical uncertainty, including the inherent soil variability, measurement error and transformation uncertainty, full probabilistic analyses should be implemented to directly consider the site-specific variability. This paper presents the procedure of developing fragility curves for levee slope stability and piping as failure mechanisms that lead to larger breaches, where a direct influence of the flood event intensity on the probability of failure is calculated. A range of fragility curve sets is presented, considering the variability of levee material properties and varying durations of the flood event, thus providing crucial insight into the vulnerability of the levee exposed to rising water levels. The procedure is applied to the River Drava levee, a site which has shown a continuous trend of increased water levels in recent years.


2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jiqing Du ◽  
Marie-Kristin von Wrisberg ◽  
Burak Gulen ◽  
Matthias Stahl ◽  
Christian Pett ◽  
...  

AbstractLegionella pneumophila infects eukaryotic cells by forming a replicative organelle – the Legionella containing vacuole. During this process, the bacterial protein DrrA/SidM is secreted and manipulates the activity and post-translational modification (PTM) states of the vesicular trafficking regulator Rab1. As a result, Rab1 is modified with an adenosine monophosphate (AMP), and this process is referred to as AMPylation. Here, we use a chemical approach to stabilise low-affinity Rab:DrrA complexes in a site-specific manner to gain insight into the molecular basis of the interaction between the Rab protein and the AMPylation domain of DrrA. The crystal structure of the Rab:DrrA complex reveals a previously unknown non-conventional Rab-binding site (NC-RBS). Biochemical characterisation demonstrates allosteric stimulation of the AMPylation activity of DrrA via Rab binding to the NC-RBS. We speculate that allosteric control of DrrA could in principle prevent random and potentially cytotoxic AMPylation in the host, thereby perhaps ensuring efficient infection by Legionella.


2017 ◽  
Vol 4 (1) ◽  
pp. 205395171769075 ◽  
Author(s):  
Andrew Schrock ◽  
Gwen Shaffer

Government officials claim open data can improve internal and external communication and collaboration. These promises hinge on “data intermediaries”: extra-institutional actors that obtain, use, and translate data for the public. However, we know little about why these individuals might regard open data as a site of civic participation. In response, we draw on Ilana Gershon to conceptualize culturally situated and socially constructed perspectives on data, or “data ideologies.” This study employs mixed methodologies to examine why members of the public hold particular data ideologies and how they vary. In late 2015 the authors engaged the public through a commission in a diverse city of approximately 500,000. Qualitative data was collected from three public focus groups with residents. Simultaneously, we obtained quantitative data from surveys. Participants’ data ideologies varied based on how they perceived data to be useful for collaboration, tasks, and translations. Bucking the “geek” stereotype, only a minority of those surveyed (20%) were professional software developers or engineers. Although only a nascent movement, we argue open data intermediaries have important roles to play in a new political landscape.


2014 ◽  
Vol 70 (a1) ◽  
pp. C207-C207
Author(s):  
Yun Chen ◽  
Shu Feng ◽  
Katsuhiko Kamada ◽  
Han Wang ◽  
Kai Tang ◽  
...  

As a typical endoribonuclease, YoeB mediates cellular adaptation in diverse bacteria by degrading mRNAs on its activation. Although the catalytic core of YoeB is thought to be identical to well-studied nucleases, this enzyme specifically targets mRNA substrates that are associated with ribosomes in vivo. However, the molecular mechanism of mRNA recognition and cleavage by YoeB, and the requirement of ribosome for its optimal activity, largely remain elusive. Here, we report the structure of YoeB bound to 70S ribosome in pre-cleavage state, revealing that both the 30S and 50S subunits participate in YoeB binding. The mRNA is recognized by the catalytic core of YoeB, of which the general base/acid (Glu46/His83) are within hydrogen-bonding distance to their reaction atoms, demonstrating an active conformation of YoeB on ribosome. Also, the mRNA orientation involves the universally conserved A1493 and G530 of 16S rRNA. In addition, mass spectrometry data indicated that YoeB cleaves mRNA following the second position at the A-site codon, resulting in a final product with a 3'–phosphate at the newly formed 3' end. Our results demonstrate a classical acid-base catalysis for YoeB-mediated RNA hydrolysis and provide insight into how the ribosome is essential for its specific activity.


Author(s):  
Steven Carr

The rise of the American motion picture corresponds to the influx of immigrants from Eastern and Southern Europe. Just as many of these immigrants initially settled in East Coast and Midwest cities, both movies and movie audiences emerged there as an urban phenomenon. Rather than view this phenomenon only in terms of the images that films of this era offered, this chapter proposes to move beyond a “reflection paradigm” of film history. Of course, film texts reflected immigrant, ethnic, and racial identities. But these identities also existed beyond the text, across movies and movie-going, and embedded within diffuse, multiple, and overlapping networks of imagined relationships. Using Bakhtin’s concept of the chronotope, this chapter recounts some preliminary case studies involving race, ethnicity, and immigration to explore how future research in this area might probe the cultural practices of movie-going among diverse audiences during the first half of the twentieth century.


Author(s):  
Yllka Azemi ◽  
Wilson Ozuem

The consequences of social media applications are experienced at every level in an organizational context, but they are arguably most extensively experienced in developing effective marketing communication strategies. Drawing on constructivist perspectives, recognizing the socially constructed realities embedded within this evolving techno-cultural construct, the current chapter suggests that understanding social media must begin not in the technological domain, but in the way in which users negotiate meanings between and amongst themselves in the Internet ecosystem. This could potentially help marketers to develop effective marketing communications programmes. Understanding the co-evolution of social media connectivity and sociality in the context of the emerging culture provides deeper insight into how SMEs, particularly in transition economies, could adapt to and contextualize values of openness and connectedness offered by this technological tapestry. Certain conditions in which the deployment of cultural transformation is likely to succeed are identified, and a future empirical research agenda is suggested.


2019 ◽  
Vol 116 (16) ◽  
pp. 7813-7818 ◽  
Author(s):  
Jie Zhou ◽  
Laura Lancaster ◽  
John Paul Donohue ◽  
Harry F. Noller

The elongation factor G (EF-G)–catalyzed translocation of mRNA and tRNA through the ribosome is essential for vacating the ribosomal A site for the next incoming aminoacyl-tRNA, while precisely maintaining the translational reading frame. Here, the 3.2-Å crystal structure of a ribosome translocation intermediate complex containing mRNA and two tRNAs, formed in the absence of EF-G or GTP, provides insight into the respective roles of EF-G and the ribosome in translocation. Unexpectedly, the head domain of the 30S subunit is rotated by 21°, creating a ribosomal conformation closely resembling the two-tRNA chimeric hybrid state that was previously observed only in the presence of bound EF-G. The two tRNAs have moved spontaneously from their A/A and P/P binding states into ap/P and pe/E states, in which their anticodon loops are bound between the 30S body domain and its rotated head domain, while their acceptor ends have moved fully into the 50S P and E sites, respectively. Remarkably, the A-site tRNA translocates fully into the classical P-site position. Although the mRNA also undergoes movement, codon–anticodon interaction is disrupted in the absence of EF-G, resulting in slippage of the translational reading frame. We conclude that, although movement of both tRNAs and mRNA (along with rotation of the 30S head domain) can occur in the absence of EF-G and GTP, EF-G is essential for enforcing coupled movement of the tRNAs and their mRNA codons to maintain the reading frame.


Humanities ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 97
Author(s):  
Jane Hoogestraat ◽  
Hillery Glasby

Invoking a dialogue between two scholars, authors Jane Hoogestraat and Hillery Glasby discuss the exigence for, construction of, and differentiation between LGBT and queer ethos. Drawing from Larry Kramer’s The Normal Heart and the construction of a gay identity, the text explores connections between queer theory, LGBT(Q) ethos, and queer futurity, ultimately arguing for a more nuanced and critical understanding of the undecidability and performativity of LGBT and queer ethos. In framing LGBT and queer ethos as being at the same time a self and socially constructed and mediated—legitimate and illegitimate—ethos can be understood not only as a site for rhetorical agency, but also as an orientation and a form of activism. Finally, the text offers a case study of Adrienne Rich’s “Yom Kippur,” which is a poem that offers a queer (and) Jewish perspective on identity—from an individual and community level—exhibiting both an LGBT and queer ethos.


Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 672 ◽  
Author(s):  
Olivia Luige ◽  
Merita Murtola ◽  
Alice Ghidini ◽  
Roger Strömberg

Peptide nucleic acid (PNA)-neocuproine conjugates have been shown to efficiently catalyse the cleavage of RNA target sequences in the presence of Cu2+ ions in a site-specific manner. These artificial enzymes are designed to force the formation of a bulge in the RNA target, the sequence of which has been shown to be key to the catalytic activity. Here, we present a further investigation into the action of Cu2+-dependent PNAzymes with respect to the dependence on bulge composition in 3- and 4-nucleotide bulge systems. Cu2+-dependent PNAzymes were shown to have a clear preference for 4-nucleotide bulges, as the cleavage of 3-nucleotide bulge-forming RNA sequences was significantly slower, which is illustrated by a shift in the half-lives from approximately 30 min to 24 h. Nonetheless, the nucleotide preferences at different positions in the bulge displayed similar trends in both systems. Moreover, the cleavage site was probed by introducing critical chemical modifications to one of the cleavage site nucleotides of the fastest cleaved 4-nucleotide RNA bulge. Namely, the exclusion of the exocyclic amine of the central adenine and the replacement of the 2′-hydroxyl nucleophile with 2′-H or 2′-OMe substituents in the RNA severely diminished the rate of RNA cleavage by the Cu2+-dependent PNAzyme, giving insight into the mechanism of cleavage. Moreover, the shorter recognition arm of the RNA/PNAzyme complex was modified by extending the PNAzyme by two additional nucleobases. The new PNAzyme was able to efficiently promote the cleavage of RNA when fully hybridised to a longer RNA target and even outperform the previous fastest PNAzyme. The improvement was demonstrated in cleavage studies with stoichiometric amounts of either PNAzyme present, and the extended PNAzyme was also shown to give turnover with a 10-fold excess of the RNA target.


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