In vitro anthelmintic activity of Chicory extracts from plants of different vegetative stages on Teladorsagia circumcincta L3

2009 ◽  
Vol 2009 ◽  
pp. 35-35
Author(s):  
Z Parrisi ◽  
S Athanasiadou ◽  
J G M Houdijk ◽  
I Kyriazakis
Keyword(s):  
Host Resistance ◽  
Production Systems ◽  
Parasitic Infection ◽  
Plant Secondary Metabolites ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematode ◽  
Teladorsagia Circumcincta ◽  
Alternative Means ◽  
Sheep Production

The consumption of plants rich in plant secondary metabolites has been associated with improved resilience of parasitised hosts, i.e. their ability to perform under parasitic infection, compared to animals fed conventional feeds (Athanasiadou et al., 2007). Furthermore, such bio-active plants can improve host resistance, which refers to the host ability to regulate gastrointestinal nematode establishment, development, fecundity and survival (Athanasiadou et. al. 2008). Chichorium intybus L. (chicory) is such a bio-active forage and its potential anthelmintic activity is currently investigated as an alternative means to control parasitism in sheep production systems. In the present study we employed an in vitro assay to study a possible mechanism of anti-parasitic action arising from chicory, and to test whether this anthelmintic activity is affected by the vegetative stage of the plant.

In vitro anthelmintic activity and safety of different plant species against the ovine gastrointestinal nematode Teladorsagia circumcincta

2019 ◽  
Vol 123 ◽  
pp. 153-158 ◽  
Author(s):  
Myriam Esteban-Ballesteros ◽  
Jaime Sanchis ◽  
Camino Gutiérrez-Corbo ◽  
Rafael Balaña-Fouce ◽  
Francisco A. Rojo-Vázquez ◽  
...  
Keyword(s):  
Plant Species ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematode ◽  
Teladorsagia Circumcincta

scholarly journals In vitro Anthelmintic Activity of Two Aloe-derived Active Principles against Sheep Gastrointestinal Nematodes

2017 ◽  
Vol 12 (12) ◽  
pp. 1934578X1701201
Author(s):  
Gianluca Fichi ◽  
Matteo Mattellini ◽  
Elisa Meloni ◽  
Guido Flamini ◽  
Stefania Perrucci
Keyword(s):  
Larval Development ◽  
Larval Mortality ◽  
Plant Secondary Metabolites ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Egg Hatch ◽  
Reference Drug ◽  
Faecal Samples ◽  
Aloe Emodin

The in vitro anthelmintic activity on sheep gastrointestinal strongyle (GIS) eggs and larvae of 0.5% aloin and 0.1% aloe-emodin was investigated. From fresh faecal samples collected by ewes naturally infected by Haemonchus, Trichostrongylus and Teladorsagia nematodes, GIS eggs were isolated and cultivated in Petri dishes (100 eggs/dish). For the in vitro evaluation of the anthelmintic activity of tested compounds, the Egg hatch test (EHT), the Larval development test (LDT) and the Larval mortality/paralysis test (LMT) were used. In each assay, the activity of tested compounds was compared to untreated and treated (0.1% thiabendazole, TBZ) controls. Six repetitions were made through the experiment. Obtained data were statistically elaborated using the X2 test. In EHT, 0.5% aloin gave highly significantly different (P<0.01) results from the untreated controls. In LDT, both 0.1% aloe-emodin and 0.5% aloin almost completely prevented the larval development from L1 to L3, showing no significant differences (P<0.01) when compared to TBZ. In LMT, larval mortality observed in 0.5% aloin treated plates was significantly higher (P<0.01) than that observed in TBZ treated controls. These results show the in vitro anthelmintic properties on sheep GIS of the examined plant secondary metabolites. In LDT and/or LMT, the activity of 0.5% aloin and 0.1% aloe-emodin was comparable to or higher than that of the reference drug.

Evaluation of anthelmintic activity of biologically synthesized silver nanoparticles against the gastrointestinal nematode, Haemonchus contortus

2016 ◽  
Vol 91 (4) ◽  
pp. 454-461 ◽  
Author(s):  
R.S. Tomar ◽  
S. Preet
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematode ◽  
Experimental Plant ◽  
Egg Hatch ◽  
Transmission Electron ◽  
Motility Inhibition

AbstractThe present study focuses on the in vitro anthelmintic activity of silver nanoparticles (AgNPs) synthesized using the aqueous extract of Azadirachta indica against Haemonchus contortus. The synthesized AgNPs were characterized by ultraviolet–visible (UV-Vis) spectrophotometry, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD) studies. The UV-Vis spectrum exhibited a sharp peak at 420 nm, which was validated by electron microscopy, indicating the preparation of spherical nanoparticles measuring 15–25 nm in size. The in vitro study was based on an egg hatch assay (EHA) and adult motility inhibition assays. Six concentrations of AgNPs were used for EHA, ranging from 0.00001 to 1.0 μg/ml, and a range of 1–25 μg/ml was used for adult worms. The highest concentration induced 85 ± 2.89% egg hatch inhibition. The IC50 value for EHA was 0.001 μg/ml, whereas in vitro adult H. contortus motility inhibition was produced at 7.89 μg/ml (LC50). The effectiveness of A. indica leaf extract (aqueous) was also evaluated, which showed an IC50 value for EHA of 115.67 μg/ml, while the LC50 against adult H. contortus was 588.54 μg/ml. The overall findings of the present study show that the experimental plant extract contains reducing properties for the synthesis of AgNPs which, in turn, showed potent anthelmintic properties. This is the first report where AgNPs have been tested for their anthelmintic properties in an in vitro model.

scholarly journals Effectiveness of flor-de-seda and pomegranate aqueous extracts on eggs of the Heterakoidea Superfamily isolated from naturally infected japanese quails

2020 ◽  
Vol 21 ◽  
Author(s):  
Patrícia Araujo BRANDÃO ◽  
David Rwbystanne Pereira da SILVA ◽  
Giuliana Garcia DINIZ ◽  
Vinícius Longo Ribeiro VILELA
Keyword(s):  
Anthelmintic Activity ◽  
Gastrointestinal Nematode ◽  
Punica Granatum ◽  
Positive Control ◽  
Control Treatment ◽  
Calotropis Procera ◽  
Aqueous Extracts ◽  
Similar Action ◽  
Japanese Quails

ABSTRACT The present study aimed to evaluate the in vitro anthelmintic efficacy of flor-de-seda (Calotropis procera S.W.) and pomegranate (Punica granatum L.) extracts on gastrointestinal nematode eggs of Japanese quails. Stool samples were collected from a herd of 5,000 adult birds raised on the floor at a commercial farm in the state of Ceará, Brazil. The samples were packed in plastic bags, kept refrigerated and transported for laboratory analysis. The eggs were placed in 2.5 ml of the suspension and 2.5 ml of flor-de-seda and pomegranate extracts in concentrations of 50; 25; 12.5; 6.25 and 3.12%. Readings were subsequently taken at 24, 48 and 72 hours. The aqueous pomegranate extract at 25 and 50% was more efficient on the larvae hatching. The flor-de-seda aqueous extract in both concentrations and evaluation periods showed similar action to that of the positive control treatment (Albendazole 10%). The pomegranate and flor-de-seda aqueous extracts have anthelmintic activity on the development of larvae in eggs of the Heterakoidea Superfamily and are therefore presented as an alternative to replace the drugs commonly used to control helminths in Japanese quails.

Anthelmintic activity of plants against gastrointestinal nematodes of goats: a review

Parasitology ◽  
2019 ◽  
Vol 146 (10) ◽  
pp. 1233-1246 ◽  
Author(s):  
Francianne Oliveira Santos ◽  
Amanda Ponce Morais Cerqueira ◽  
Alexsandro Branco ◽  
Maria José Moreira Batatinha ◽  
Mariana Borges Botura
Keyword(s):  
Animal Feed ◽  
Chemical Constituents ◽  
Plant Secondary Metabolites ◽  
Herbal Medicines ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Free Living ◽  
Anthelmintic Effect

AbstractThe gastrointestinal nematodes (GIN) stand out as an important cause of disease in small ruminant, especially on goat farm. Widespread resistance to synthetic anthelminthics has stimulated the research for alternative strategies of parasite control, including the use of medicinal plants. The present work summarizes the in vitro and in vivo studies of plants with activity against GIN of goats, focusing on the description of chemical constituents related to this effect. This review retrieved 56 scientific articles from 2008 to 2018 describing more than 100 different plant species. The most frequently investigated family was Fabaceae (30.7%). Most in vitro studies on the activity of plant extracts and fractions were carried out with of free-living stages nematodes. In vivo studies were conducted mainly with the use of plants in animal feed and generally showed lower effectiveness compared to in vitro assays. The main plant secondary metabolites associated with anthelmintic effect are condensed tannins, saponin and flavonoids. However, the studies with compounds isolated from plants and elucidation of their mechanisms of action are scarce. Herbal medicines are thought to be promising sources for the development of effective anthelmintic agents.

scholarly journals Infection with the sheep gastrointestinal nematode Teladorsagia circumcincta increases luminal pathobionts

2019 ◽  
Author(s):  
Alba Cortes ◽  
John Wills ◽  
Xiaopei Su ◽  
Rachel Hewitt ◽  
Jack Robertson ◽  
...  
Keyword(s):  
T Cells ◽  
Control Strategies ◽  
Parasitic Infection ◽  
Gastrointestinal Nematode ◽  
Parasite Infection ◽  
Helminth Parasites ◽  
Gastrointestinal Helminths ◽  
Microbial Composition ◽  
Teladorsagia Circumcincta ◽  
The Impact

Abstract Background The multifaceted interactions between gastrointestinal (GI) helminth parasites, vertebrate host gut microbiota and the immune system are emerging as a key area of research within the field of host-parasite relationships. In spite of the plethora of data available on the impact that GI helminths exert on the composition of the gut microflora, whether alterations of microbial profiles are caused by direct parasite-bacteria interactions or, indirectly, by alterations of the GI environment (e.g. mucosal immunity), remains to be determined. Furthermore, no data is thus far available on the downstream roles that qualitative and quantitative changes in gut microbial composition play in the overall pathophysiology of parasite infection and disease. Results In this study, we investigated the fluctuations in microbiota composition and local immune microenvironment of sheep vaccinated against, and experimentally infected with, the ‘brown stomach worm’ Teladorsagia circumcincta, a parasite of worldwide socio-economic significance. We compared gut microbial profiles with those obtained from groups of unvaccinated/infected and unvaccinated/uninfected animals. We show that alterations to the gut microbial composition are associated mainly with parasite infection, and that this involves the expansion of populations of bacteria with known pro-inflammatory roles that may contribute to the immunopathology of helminth disease. Using novel quantitative approaches for the analysis of confocal microscopy-derived images we also show that gastric tissue infiltration of T cells is driven by parasitic infection rather than anti-helminth vaccination. Conclusions Teladorsagia circumcincta infection leads to an expansion of potentially pro-inflammatory gut microbial species and abomasal T cells. This data paves the way for future experiments aimed at determining the contribution of the gut flora to the pathophysiology of parasitic disease, with the ultimate aim to design and develop novel treatment/control strategies focused on preventing and/or restricting bacterial-mediated inflammation upon infection by gastrointestinal helminths.

scholarly journals A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortus

2018 ◽  
Vol 27 (4) ◽  
pp. 473-480 ◽  
Author(s):  
Ledia Feitosa Wanderley ◽  
Alexandra Martins dos Santos Soares ◽  
Carolina Rocha e Silva ◽  
Isaias Moreira de Figueiredo ◽  
Andre Teixeira da Silva Ferreira ◽  
...  
Keyword(s):  
Proteolytic Activity ◽  
Cysteine Protease ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematode ◽  
Bioactive Molecules ◽  
Promising Alternative ◽  
Ficus Benjamina ◽  
Control Programmes

Abstract Haemonchus contortus is a gastrointestinal nematode that is responsible for high mortality rates in ruminant herds. The resistance of nematodes to synthetic anthelmintics is widespread and requires a continuous search for new bioactive molecules, such as proteins. The objective of this study was to evaluate the anthelmintic potential of a protease purified from the latex of Ficus benjamina against H. contortus . Fresh latex was collected from plants via small incisions in the green stems, the rubber was removed by centrifugation, and the latex protein extract (LPE) was obtained. After LPE fractionation with ammonium sulfate and chromatography of the fraction containing the highest proteolytic activity on CM-cellulose, a cysteine protease (FbP) was purified. FbP has a molecular mass of approximately 23.97 kDa, and its proteolytic activity was stable between pH 6.0 and pH 10 and over a broad temperature range, with optimum activity at 60 °C. FbP inhibited both the development and exsheathment of H. contortus larvae, with 50% effective concentrations of 0.26 and 0.79 mg/mL, respectively. We conclude that this cysteine protease from F. benjamina latex with anthelmintic activity against H. contortus could be a promising alternative for the development of products for use in parasite control programmes.

Animal grazing selectivity and plant chemistry issues impact on the potential of Rhagodia preissii as an anthelmintic shrub

Parasitology ◽  
2011 ◽  
Vol 138 (5) ◽  
pp. 628-637 ◽  
Author(s):  
A. C. KOTZE ◽  
E. N. ZADOW ◽  
P. E. VERCOE ◽  
N. PHILLIPS ◽  
A. TOOVEY ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Plant Extracts ◽  
Plant Material ◽  
Plant Secondary Metabolites ◽  
Anthelmintic Activity ◽  
Sheep Grazing ◽  
Plant Chemistry ◽  
Animal Grazing ◽  
Selection Of

SUMMARYAs Rhagodia preissii had shown significant in vitro anthelmintic activity in a previous study, we examined the effect of including this shrub in the diet of sheep infected with Trichostrongylus colubriformis. Worm-infected merino wethers were grazed for 7 weeks on either R. preissii or annual pasture, and faecal egg counts (FECs) were conducted weekly. Plant material was collected weekly from eaten and uneaten plants, and analysed for levels of plant secondary metabolites (tannins, oxalates, saponins) and in vitro anthelmintic activity. While mean FECs were consistently lower in sheep grazing R. preissii compared to pasture (reductions of 20–74%), the differences were not significant. There was no relationship between grazing preference (eaten or uneaten) and in vitro anthelmintic activity of plant extracts. The levels of saponins and oxalates did not correlate with grazing preference or in vitro anthelmintic activity, while tannins were not responsible for the anthelmintic activity. While the identity of the grazing deterrent and in vitro anthelmintic compounds remain unknown, the presence of plants which were both highly preferred by the sheep and showed in vitro anthelmintic activity indicates a potential to develop the species as an anthelmintic shrub through selection of shrub populations dominated by such plants.

scholarly journals Is there a role for chicory in controlling internal parasitism in organic sheep?

2005 ◽  
Vol 2005 ◽  
pp. 88-88
Author(s):  
S. Athanasiadou ◽  
D. Gray ◽  
O. Tzamaloukas ◽  
K. Zaralis ◽  
T. Lhuillier ◽  
...  
Keyword(s):  
Production Systems ◽  
Cichorium Intybus ◽  
Alternative Means ◽  
Faecal Egg Counts ◽  
Sheep Production

The potential antiparasitic effects of chicory (Cichorium intybus ) are currently investigated as an alternative means to control parasitism in organic sheep production systems. Previous studies showed that parasitised lambs grazing on parasite-clean chicory swards had improved growth and lower faecal egg counts (FEC) compared to those grazing on parasite-clean grass pastures (Athanasiadou et al, 2004). The objective of this experiment was to investigate whether chicory can have a role as a potential means to control parasitism in lactating ewes and their lambs grazing on previously parasite contaminated pastures.

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