In Situ Real-Time Study on Dynamics of Microbially Induced Calcium Carbonate Precipitation at a Single-Cell Level

2018 ◽  
Vol 52 (16) ◽  
pp. 9266-9276 ◽  
Author(s):  
Wenchao Zhang ◽  
Ying Ju ◽  
Yiwu Zong ◽  
Hao Qi ◽  
Kun Zhao
Keyword(s):  
Calcium Carbonate ◽  
Single Cell ◽  
Real Time ◽  
Carbonate Precipitation ◽  
Single Cell Level ◽  
Time Study ◽  
Calcium Carbonate Precipitation ◽  
Cell Level

scholarly journals Microbial-induced calcium carbonate precipitation: an experimental toolbox for in situ and real time investigation of micro-scale pH evolution

RSC Advances ◽  
2020 ◽  
Vol 10 (35) ◽  
pp. 20485-20493
Author(s):  
Jennifer Zehner ◽  
Anja Røyne ◽  
Alexander Wentzel ◽  
Pawel Sikorski
Keyword(s):  
Calcium Carbonate ◽  
Real Time ◽  
Experimental Methods ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Micro Scale ◽  
Ph Changes ◽  
Global And Local ◽  
Local Ph

We present two novel experimental methods to follow global and local pH changes on a microscale in bio-cementation processes.

scholarly journals Microbial-induced calcium carbonate precipitation: An experimental toolbox for in situ and real-time investigation of micro-scale pH evolution

2020 ◽  
Author(s):  
Jennifer Zehner ◽  
Anja Røyne ◽  
Alexander Wentzel ◽  
Pawel Sikorski
Keyword(s):  
Calcium Carbonate ◽  
Real Time ◽  
Small Sample ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Micro Scale ◽  
Ph Changes ◽  
Macro Scale ◽  
Local Ph

AbstractConcrete is the second most consumed product by humans, after water. However, the production of cement, which is used as a binding material in concrete, causes more than 5% of anthropogenic CO2 emissions and has therefore a significant contribution to climate change and global warming. Due to increasing environmental awareness and international climate goals, there is a need for emission-reduced materials, that can replace conventional concrete in certain applications. One path to produce a solid, concrete-like construction material is microbial-induced calcium carbonate precipitation (MICP). As a calcium source in MICP, crushed limestone, which mainly consists out of CaCO3, can be dissolved with acids, for example lactic acid. The pH evolution during crystallization and dissolution processes provides important information about kinetics of the reactions. However, previous research on MICP has mainly been focused on macro-scale pH evolution and on characterization of the finished material. To get a better understanding of MICP it is important to be able to follow also local pH changes in a sample. In this work we present a new method to study processes of MICP at micro-scale in situ and in real time. We present two different methods to monitor the pH changes during the precipitation process of CaCO3. In the first method, the average pHs of small sample volumes are measured in real time, and pH changes are subsequently correlated with processes in the sample by comparing to optical microscope results. The second method is introduced to follow local pH changes at a grain scale in situ and in real time. Furthermore, local pH changes during the dissolution of CaCO3 crystals are monitored. We demonstrate that these two methods are powerful tools to investigate pH changes for both MICP precipitation and CaCO3 dissolution for knowledge-based improvement of MICP-based material properties.Graphical TOC Entry

Development of a Direct In Situ PCR Method for Detection of Specific Bacteria in Natural Environments

1998 ◽  
Vol 64 (4) ◽  
pp. 1536-1540 ◽  
Author(s):  
Katsuji Tani ◽  
Ken Kurokawa ◽  
Masao Nasu
Keyword(s):  
Alkaline Phosphatase ◽  
Single Cell ◽  
Natural Environments ◽  
Single Cell Level ◽  
Bacterial Cells ◽  
Cell Level ◽  
In Situ Pcr ◽  
Glass Slides ◽  
Pcr Products

ABSTRACT We applied HNPP (2-hydroxy-3-naphthoic acid-2′-phenylanilide phosphate) to direct in situ PCR for the routine detection of specific bacterial cells at the single-cell level. PCR was performed on glass slides with digoxigenin-labeled dUTP. The digoxigenin-labeled PCR products were detected with alkaline phosphatase-labeled antidigoxigenin antibody and HNPP which was combined with Fast Red TR. A bright red fluorescent signal was produced from conversion to HNP (dephosphorylated form) by alkaline phosphatase. We used the ECOL DNA primer set for amplification of ribosomal DNA of Escherichia coli to identify cells specifically at the single-cell level in a bacterial mixture. High-contrast images were obtained under an epifluorescence microscope with in situ PCR. By image analysis,E. coli cells in polluted river water also were detected.

scholarly journals Transfer of a Phage T4 Gene into Enterobacteriaceae, Determined at the Single-Cell Level

2009 ◽  
Vol 76 (4) ◽  
pp. 1274-1277 ◽  
Author(s):  
Takehiko Kenzaka ◽  
Masao Nasu ◽  
Katsuji Tani
Keyword(s):  
Gene Transfer ◽  
Single Cell ◽  
Dna Amplification ◽  
Single Cell Level ◽  
Cell Level ◽  
Phage T4 ◽  
Gene Copies ◽  
Amplification Technique

ABSTRACT The transfer range of phage genes was investigated at the single-cell level by using an in situ DNA amplification technique. After absorption of phages, a phage T4 gene was maintained in the genomes of non-plaque-forming bacteria at frequencies of 10−2 gene copies per cell. The gene transfer decreased the mutation frequencies in nonhost recipients.

Measurement of Molecular Mobility in Mammalian Cells

2004 ◽  
Author(s):  
Alptekin Aksan ◽  
Mehmet Toner
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Chemical Reaction ◽  
Molecular Mobility ◽  
Mammalian Cells ◽  
Free Water ◽  
Single Cell Level ◽  
Extracellular Water ◽  
Cell Level

Preservation of mammalian cells requires establishing a reversible stasis condition by reducing the intra/extracellular molecular mobility ensuring reduced chemical reaction and deterioration rates. Molecular mobility may be reduced by various techniques. For example, in cryopreservation, mobility within and surrounding the cell is reduced through freezing the free water that constitutes 70–90% of the cell’s composition. In dried-state preservation applied successfully to preserve seeds, pharmacological materials and foodstuff (mimicking the anhydrobiosis phenomenon seen in nature), reduction in molecular mobility is established by removing intra/extracellular water. Certain carbohydrates (such as trehalose and sucrose) can be artificially uploaded into mammalian cells to replace the removed water and to form an intra/extracellular glass. In this research, a fluorescent rotor is utilized to determine the changes in intracellular molecular mobility during carbohydrate uploading of mammalian cells. It was shown that using this technique, it is feasible to make real-time mobility measurements at a single cell level.

scholarly journals Visualizing and isolating iron-reducing microorganisms at single cell level

2020 ◽  
Author(s):  
Cuifen Gan ◽  
Rongrong Wu ◽  
Yeshen Luo ◽  
Jianhua Song ◽  
Dizhou Luo ◽  
...  
Keyword(s):  
Single Cell ◽  
Iron Reduction ◽  
High Sensitivity ◽  
Ex Situ ◽  
Single Cell Level ◽  
Cell Level ◽  
Cell Sorter ◽  
Fluorescent Intensity

AbstractIron-reducing microorganisms (FeRM) play key roles in many natural and engineering processes. Visualizing and isolating FeRM from multispecies samples are essential to understand the in-situ location and geochemical role of FeRM. Here, we visualized FeRM by a “turn-on” Fe2+-specific fluorescent chemodosimeter (FSFC) with high sensitivity, selectivity and stability. This FSFC could selectively identify and locate active FeRM from either pure culture, co-culture of different bacteria or sediment-containing samples. Fluorescent intensity of the FSFC could be used as an indicator of Fe2+ concentration in bacterial cultures. By integrating FSFC with a single cell sorter, we obtained three FSFC-labeled cells from an enriched consortia and all of them were subsequently evidenced to be capable of iron-reduction and two unlabeled cells were evidenced to have no iron-reducing capability, further confirming the feasibility of the FSFC.ImportanceVisualization and isolation of FeRM from samples containing multispecies are commonly needed by researchers from different disciplines, such as environmental microbiology, environmental sciences and geochemistry. However, no available method has been reported. In this study, we provid a solution to visualize FeRM and evaluate their activity even at single cell level. Integrating with single cell sorter, FeRM can also be isolated from samples containing multispecies. This method can be used as a powerful tool to uncover the in-situ or ex-situ role of FeRM and their interactions with ambient microbes or chemicals.

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