UPLC-ESI-TOF MS-Based Metabolite Profiling of the Antioxidative Food Supplement Garcinia buchananii

Cold atmospheric plasma (CAP) is a novel technology, which has been widely applied in biomedicine, especially in wound healing, dermatological treatment, hemostasis, and cancer treatment. In most cases, CAP treatment will interact with innumerable blood capillaries. Therefore, it is important and necessary to understand the effects of CAP treatment on endothelial cell metabolism. In this study, the metabolite profiling of plasma treatment on endothelial cells was measured by gas chromatography tandem time-of-flight mass spectrometry (GC-TOF-MS). We found that 695 signals (metabolites) were detected by GC-TOF-MS and then evaluated using orthogonal projections to latent structures discriminant analysis (OPLS-DA). All the differential metabolites were listed, and proline and xanthosine were the two of the most downregulated metabolites by plasma treatment. By comprehensive metabolic pathway analysis with the KEGG pathway, we showed that alanine, aspartate, glutamate, and purine metabolism pathways were the most significantly suppressed after gas plasma treatment in human endothelial cells. Our finding gives an overall picture of the metabolic pathways affected by plasma treatment in endothelial cells.

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Metabolite profiling of RCS-4, a novel synthetic cannabinoid designer drug, using human hepatocyte metabolism and TOF-MS

Bioanalysis ◽

10.4155/bio.14.13 ◽

2014 ◽

Vol 6 (11) ◽

pp. 1471-1485 ◽

Cited By ~ 13

Author(s):

Adarsh S Gandhi ◽

Mingshe Zhu ◽

Shaokun Pang ◽

Ariane Wohlfarth ◽

Karl B Scheidweiler ◽

...

Keyword(s):

Metabolite Profiling ◽

Human Hepatocyte ◽

Synthetic Cannabinoid ◽

Designer Drug ◽

Hepatocyte Metabolism ◽

Tof Ms

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Metabolite profiling of Zi-Shen pill in rat biological specimens by UPLC-Q-TOF/MS

Chinese Journal of Natural Medicines ◽

10.1016/s1875-5364(15)60019-x ◽

2015 ◽

Vol 13 (2) ◽

pp. 145-160 ◽

Cited By ~ 3

Author(s):

Xiao-Wei LIU ◽

Feng ZHANG ◽

Shou-Hong GAO ◽

Bo JIANG ◽

Wan-Sheng CHEN

Keyword(s):

Metabolite Profiling ◽

Tof Ms

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Optimization of Metabolite Extraction Protocols for Untargeted Metabolite Profiling of Mycoparasitic Scytalidium parasiticum using LC-TOF-MS

Sains Malaysiana ◽

10.17576/jsm-2018-4712-16 ◽

2018 ◽

Vol 47 (12) ◽

pp. 3061-3068 ◽

Cited By ~ 1

Author(s):

Choon Kiat Lim ◽

Rafidah Ahmad ◽

Nurul Fadhilah Marzuki ◽

Yit Kheng Goh ◽

Kamalrul Azlan Azizan ◽

...

Keyword(s):

Metabolite Profiling ◽

Untargeted Metabolite Profiling ◽

Metabolite Extraction ◽

Tof Ms

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A UPLC-Q-TOF/MS-Based Metabolomics Study on the Effect of Corallodiscus flabellatus (Craib) B. L. Burtt Extract on Alzheimer’s Disease

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/8868690 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Yang-Yang Wang ◽

Ning Zhou ◽

Yan-Po Si ◽

Zhi-Yao Bai ◽

Meng Li ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Metabolite Profiling ◽

Huperzine A ◽

Control Group ◽

Model Group ◽

Metabolomics Study ◽

Samp8 Mice ◽

Senescence Accelerated Mouse ◽

Tof Ms

A UPLC-Q-TOF/MS-based metabolomics study was carried out to explore the intervening mechanism of Corallodiscus flabellatus (Craib) B. L. Burtt (CF) extract on Alzheimer’s disease (AD). The AD model group consisted of senescence-accelerated mouse prone 8 (SAMP8) mice, and the control group consisted of senescence-accelerated mouse resistant 1 (SAMR1) mice. UPLC-Q-TOF/MS detection, multivariate statistical analysis, and pathway enrichment were jointly performed to research the change in metabolite profiling in the urine of AD mice. The result suggested that the metabolite profiling of SAMP8 mice significantly changed at the sixth month compared with SAMR1 mice of the same age, and the principal component analysis (PCA) score scatter plots of the CF group closely resembled those of the control and positive drug (huperzine A, HA) group. A total of 28 metabolites were considered potential biomarkers associated with the metabolism of beta-alanine, glycine, serine, threonine, cysteine, methionine, arginine, proline, and purines in AD mice. Furthermore, the CF group was clustered with the control and positive group and was clearly separated from the model group in the heat map. In conclusion, significant anti-AD effects were firstly observed in mice after treatment with the CF extract, and the urinary metabolomics approach assisted with dissecting the underlying mechanism.

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Metabolite Profiling of Dihydroartemisinin in Blood of Plasmodium-Infected and Healthy Mice Using UPLC-Q-TOF-MSE

Frontiers in Pharmacology ◽

10.3389/fphar.2020.614159 ◽

2021 ◽

Vol 11 ◽

Author(s):

Yifan Zhao ◽

Peng Sun ◽

Yue Ma ◽

Xiaoqiang Chang ◽

Xingyu Chen ◽

...

Keyword(s):

Blood Plasma ◽

Red Blood Cells ◽

Metabolic Pathway ◽

Blood Cells ◽

Metabolite Profiling ◽

First Line ◽

First Time ◽

Insight Into ◽

Tof Ms

Dihydroartemisinin (DHA) and its’ derivatives have been employed as the most powerful first-line drugs for malarial treatment for several decades. The metabolism of DHA has not been studied clearly. Previous reports were focused on the pharmacokinetics procedure of DHA in healthy rats. The metabolites of DHA in red blood cells (RBC), especially in the RBC from Plasmodium-infected models, have rarely been studied. The Plasmodium species parasitize inside RBC, and these cells should be the final place where DHA performs its activity. In this study, the profile of DHA metabolites in biosample (blood, plasma, and RBC) of the infected and healthy mice was investigated with UPLC-Q-TOF-MS and UNIFI platform to gain insight into DHA metabolism. Results show that a total of 25 metabolites were successfully identified in infected (30 in healthy) blood, 27 in infected (27 in healthy) plasma, and 15 in infected (22 in healthy) RBC. Results show that hydroxylation, OH-dehydration, and glucuronidation reactions were important in the metabolic pathway in vivo. Significantly, DHA metabolites inside RBC were identified for the first time. 8-Hydroxy (8-OH) DHA, 4α-OH deoxy ART, and 6β-OH deoxy ART were identified in vivo for the first time.

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