Green synthesis of nanoparticles is the recent and advanced technology in the fields of nanoscience and biotechnology which produces the eco-friendly and more efficient antimicrobial agents. Therefore, present study reported a simple, suitable and cost effective method of preparation of bioactive antimicrobial agents by the green strategy of copper oxide nanoparticles with Moringa oleifera through a green route method. In this green process the vital and varsatile medicinal quality plant i.e., Moringa oleifera leaves extract as a reducing agent as well as stabilizing agent were used to prepare the copper oxide nanoparticles. The synthesized copper oxide nanoparticles were characterized by UV- Vis, SEM, and TEM techniques. The antibacterial activity of the samples were tested by disc diffusion method against gram positive bacteria (S.aureus), gram negative bacteria (E.coli ). The size of synthesized copper oxide nanoparticles was in the ranges around 60-100 nm with dot and spherical shaped morphology. The antimicrobial activities of biofunctionalized copper oxide nanoparticles were observed significant inhibition activity. From this analysis, the green synthesized biofunctionalization method what we have suggested is exposed assure results in the antibacterial activity tests. Moreover, biofunctionalized copper oxide nanoparticles are shown superior performance in the opposed to of microbes.
