Interface-Targeting Strategy Enables Two-Photon Fluorescent Lipid Droplet Probes for High-Fidelity Imaging of Turbid Tissues and Detecting Fatty Liver

2018 ◽  
Vol 10 (13) ◽  
pp. 10706-10717 ◽  
Author(s):  
Lifang Guo ◽  
Minggang Tian ◽  
Ruiqing Feng ◽  
Ge Zhang ◽  
Ruoyao Zhang ◽  
...  
Keyword(s):  
Fatty Liver ◽  
Lipid Droplet ◽  
High Fidelity ◽  
Two Photon ◽  
Targeting Strategy ◽  
Fluorescent Lipid

Diagnosis of Fatty Liver Disease by a Bright, Multiphoton-Active and Lipid-Droplet-Specific AIEgen with Nonaromatic Rotors

2020 ◽  
Author(s):  
Hojeong Park ◽  
Shijie Li ◽  
Guangle Niu ◽  
Haoke Zhang ◽  
Zhuoyue Song ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Liver Disease ◽  
Fatty Liver ◽  
Lipid Droplet ◽  
Fatty Liver Disease ◽  
Intramolecular Charge Transfer ◽  
Stokes Shift ◽  
Transfer Effect ◽  
Photon Absorption ◽  
Two Photon

Fatty liver disease (FLD) has become an increasing global health risk. However, an accurate diagnosis of FLD at an early stage remains a great challenge due to lack of suitable imaging tools. To this end, we developed the first fluorescent two-photon aggregationinduced emission (AIE) luminogen ABCXF for high-contrast imaging of FLD tissue. ABCXF has a large Stokes shift, good two-photon absorption cross-section, bright red emission, high fluorescence quantum yield in solid state, and excellent photostability. It shows abnormal intramolecular charge transfer effect instead of twisted intramolecular charge transfer effect in polar solvents. Photophysical and crystal data demonstrated that it exhibits nonaromatic rotors - trifluoromethyto contribute to its AIE effect. Biocompatible lipid droplet-targeting ABCXF can selectively light up lesions in the FLD tissue with a high signal-to-noise ratio. It shows superior imaging performances compared to Oil Red O. Thus, ABCXF can be a powerful tool for the diagnosis and evaluation of FLD from a liver biopsy.

Diagnosis of Fatty Liver Disease by a Bright, Multiphoton-Active and Lipid-Droplet-Specific AIEgen with Nonaromatic Rotors

2020 ◽  
Author(s):  
Hojeong Park ◽  
Shijie Li ◽  
Guangle Niu ◽  
Haoke Zhang ◽  
Zhuoyue Song ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Liver Disease ◽  
Fatty Liver ◽  
Lipid Droplet ◽  
Fatty Liver Disease ◽  
Intramolecular Charge Transfer ◽  
Stokes Shift ◽  
Transfer Effect ◽  
Photon Absorption ◽  
Two Photon

Fatty liver disease (FLD) has become an increasing global health risk. However, an accurate diagnosis of FLD at an early stage remains a great challenge due to lack of suitable imaging tools. To this end, we developed the first fluorescent two-photon aggregationinduced emission (AIE) luminogen ABCXF for high-contrast imaging of FLD tissue. ABCXF has a large Stokes shift, good two-photon absorption cross-section, bright red emission, high fluorescence quantum yield in solid state, and excellent photostability. It shows abnormal intramolecular charge transfer effect instead of twisted intramolecular charge transfer effect in polar solvents. Photophysical and crystal data demonstrated that it exhibits nonaromatic rotors - trifluoromethyto contribute to its AIE effect. Biocompatible lipid droplet-targeting ABCXF can selectively light up lesions in the FLD tissue with a high signal-to-noise ratio. It shows superior imaging performances compared to Oil Red O. Thus, ABCXF can be a powerful tool for the diagnosis and evaluation of FLD from a liver biopsy.

Lysosomal targeted NIR photosensitizer for photodynamic therapy and two-photon fluorescent imaging

2021 ◽  
Author(s):  
Ruiyuan Liu ◽  
Yuping Zhou ◽  
Di Zhang ◽  
Genghan He ◽  
Chuang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
High Fidelity ◽  
Design And Synthesis ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Design and synthesis of near-infrared (NIR) emissive fluorophore for imaging of organelle and photodynamic therapy has received enormous attention. Hence, NIR emissive fluorophore of high-fidelity lysosome targeting, two-photon fluorescence imaging,...

Diagnosis of Fatty Liver Disease by a Multiphoton-Active and Lipid-Droplet-Specific AIEgen with Nonaromatic Rotors

2021 ◽  
Author(s):  
Hojeong Park ◽  
Shijie Li ◽  
Guangle Niu ◽  
Haoke Zhang ◽  
Zhuo-yue Song ◽  
...  
Keyword(s):  
Liver Disease ◽  
Global Health ◽  
Health Risk ◽  
Fatty Liver ◽  
Lipid Droplet ◽  
Fatty Liver Disease ◽  
Early Stage ◽  
Accurate Diagnosis

Fatty liver disease (FLD) has become an increasing global health risk. However, an accurate diagnosis of FLD at an early stage remains a great challenge due to the lack of...

Isolation of Micro- and Macro-Droplet Fractions from Needle Biopsy Specimens of Human Liver and Determination of the Subcellular Distribution of the Accumulating Liver Lipids in Alcoholic Fatty Liver

1984 ◽  
Vol 67 (3) ◽  
pp. 337-345 ◽  
Author(s):  
S. R. Cairns ◽  
T. J. Peters
Keyword(s):  
Fatty Liver ◽  
Lipid Droplet ◽  
Subcellular Distribution ◽  
Needle Biopsy ◽  
Density Lipoprotein ◽  
Normal Liver ◽  
Fold Increase ◽  
Bimodal Distribution ◽  
Alcoholic Fatty Liver ◽  
Biopsy Specimens

1. Needle biopsy specimens of liver were obtained from six control subjects with histologically normal liver and 11 chronic alcoholics with fatty liver. 2. Micro- and macro-lipid droplet fractions were isolated by differential flotation. These fractions, together with the sedimenting membranes, were assayed for cholesterol, cholesteryl ester, phospholipid, free fatty acids and triglyceride. 3. Electron microscopy demonstrated marked differences in the range of lipid droplet sizes in the two fractions and biochemical analysis suggested that the microdroplet lipid corresponded to pre-very low density lipoprotein (VLDL) particles. 4. Studies on biopsies from patients with alcoholic fatty liver showed a 2–3-fold increase in triglyceride in both lipid droplet fractions but most of the accumulating triglyceride was sedimentable and membrane-bound. 5. Needle biopsy specimens from two patients with alcoholic fatty liver were fractionated with a vertical pocket re-orientating rotor. The principal organelles were separated and the subcellular distribution of triglyceride, phospholipid and free cholesterol determined. Triglyceride showed a bimodal distribution to a particulate fraction tentatively located to Golgi particles and to droplet-lipid remaining in the sample layer.

scholarly journals Characterization of a new class of blue-fluorescent lipid droplet markers for live-cell imaging in plants

2015 ◽  
Vol 34 (4) ◽  
pp. 655-665 ◽  
Author(s):  
Soujanya Kuntam ◽  
László G. Puskás ◽  
Ferhan Ayaydin
Keyword(s):  
Lipid Droplet ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
New Class ◽  
Fluorescent Lipid

Exercise regulates lipid droplet dynamics in normal and fatty liver

2019 ◽  
Vol 1864 (12) ◽  
pp. 158519 ◽  
Author(s):  
Francisco Pino-de la Fuente ◽  
Laura Quezada ◽  
Carlos Sepúlveda ◽  
Matías Monsalves-Alvarez ◽  
Juan M. Rodríguez ◽  
...  
Keyword(s):  
Fatty Liver ◽  
Lipid Droplet ◽  
Droplet Dynamics
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