Recycling of Brewer’s Spent Grain as a Biosorbent by Nitro-Oxidation for Uranyl Ion Removal from Wastewater

Abstract Background Microbial surfactants called biosurfactants, thanks to their high biodegradability, low toxicity and stability can be used not only in bioremediation and oil processing, but also in the food and cosmetic industries, and even in medicine. However, the high production costs of microbial surfactants and low efficiency limit their large-scale production. This requires optimization of management conditions, including the possibility of using waste as a carbon source, such as food processing by-products. This papers describes the production and characterization of the biosurfactant obtained from the endophytic bacterial strain Bacillus pumilus 2A grown on various by-products of food processing and its potential applications in supporting plant growth. Four different carbon and nitrogen sources, pH, inoculum concentration and temperature were optimized within Taguchi method. Results Optimization of bioprocess within Taguchi method and experimental analysis revealed that the optimal conditions for biosurfactant production were brewer’s spent grain (5% w/v), ammonium nitrate (1% w/v), pH of 6, 5% of inoculum, and temperature at 30 °C, leading to 6.8 g/L of biosurfactant. Based on gas chromatography–mass spectrometry and Fourier transform infrared spectroscopy analysis produced biosurfactant was determined as glycolipid. Obtained biosurfactant has shown high and long term thermostability, surface tension of 47.7 mN/m, oil displacement of 8 cm and the emulsion index of 69.11%. The examined glycolipid, used in a concentration of 0.2% significantly enhanced growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Conclusions The endophytic Bacillus pumilus 2A produce glycolipid biosurfactant with high and long tem thermostability, what makes it useful for many purposes including food processing. The use of brewer’s spent grain as the sole carbon source makes the production of biosurfactants profitable, and from an environmental point of view, it is an environmentally friendly way to remove food processing by products. Glycolipid produced by endophytic Bacillus pumilus 2A significantly improve growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Obtained results provide new insight to the possible use of glycolipids as plant growth promoting agents.

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Potential of Brewer’s Spent Grain as a Potential Replacement of Wood in pMDI, UF or MUF Bonded Particleboard

Polymers ◽

10.3390/polym13030319 ◽

2021 ◽

Vol 13 (3) ◽

pp. 319

Author(s):

Marius Cătălin Barbu ◽

Zeno Montecuccoli ◽

Jakob Förg ◽

Ulrike Barbeck ◽

Petr Klímek ◽

...

Keyword(s):

Internal Bond ◽

Mechanical Performance ◽

Animal Feed ◽

Urea Formaldehyde ◽

Modulus Of Rupture ◽

Brewer’S Spent Grain ◽

Beer Brewing ◽

Removal Of Heavy Metals ◽

Dry Conditions ◽

Spent Grain

Brewer’s spent grain (BSG) is the richest by-product (85%) of the beer-brewing industry, that can be upcycled in a plentiful of applications, from animal feed, bioethanol production or for removal of heavy metals from wastewater. The aim of this research is to investigate the mechanical, physical and structural properties of particleboard manufactured with a mixture of wood particles and BSG gradually added/replacement in 10%, 30% and 50%, glued with polymeric diisocyanate (pMDI), urea-formaldehyde (UF) and melamine urea-formaldehyde (MUF) adhesives. The density, internal bond, modulus of rupture, modulus of elasticity, screw withdrawal resistance, thickness swelling and water absorption were tested. Furthermore, scanning electron microscopy anaylsis was carried out to analyze the structure of the panels after the internal bond test. Overall, it was shown that the adding of BSG decreases the mechanical performance of particleboard, due to reduction of the bonding between wood and BSG particles. This decrease has been associated with the structural differences proven by SEM inspection. Interaction of particles with the adhesive is different for boards containing BSG compared to those made from wood. Nevertheless, decrease in the mechanical properties was not critical for particleboards produced with 10% BSG which could be potentially classified as a P2 type, this means application in non-load-bearing panel for interior use in dry conditions, with high dimensional stability and stiffness.

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Anticholinesterase Activities of Different Solvent Extracts of Brewer’s Spent Grain

Foods ◽

10.3390/foods10050930 ◽

2021 ◽

Vol 10 (5) ◽

pp. 930

Author(s):

Rares I. Birsan ◽

Peter Wilde ◽

Keith W. Waldron ◽

Dilip K. Rai

Keyword(s):

Ethyl Acetate Fraction ◽

Therapeutic Approaches ◽

Total Polyphenol ◽

Brewer’S Spent Grain ◽

Dietary Polyphenols ◽

Synthetic Drugs ◽

Naturally Occurring ◽

Diferulic Acid ◽

Spent Grain ◽

Butyryl Cholinesterase

Cholinesterases, involved in acetylcholine catabolism in the central and peripheral nervous system, have been strongly linked with neurodegenerative diseases. Current therapeutic approaches using synthetic drugs present several side effects. Hence, there is an increasing research interest in naturally-occurring dietary polyphenols, which are also considered efficacious. Food processing by-products such as brewer’s spent grain (BSG) would be a potential bio-source of polyphenols. In this study, polyphenol-rich BSG extracts using 60% acetone and 0.75% NaOH solutions were generated, which were further subjected to liquid–liquid partitioning using various organic solvents. The water-partitioned fractions of the saponified extracts had the highest total polyphenol content (6.2 ± 2.8mgGAE/g dw) as determined by Folin–Ciocalteu reagent, while the LC-MS/MS showed ethyl acetate fraction with the highest phenolics (2.9 ± 0.3mg/g BSG dw). The best inhibitions of acetyl- (37.9 ± 2.9%) and butyryl- (53.6 ± 7.7%) cholinesterases were shown by the diethyl ether fraction of the saponified extract. This fraction contained the highest sum of quantified phenolics (99 ± 21.2µg/mg of extract), and with significant (p < 0.01) inhibitory contribution of decarboxylated-diferulic acid. Amongst the standards, caffeic acid presented the highest inhibition for both cholinesterases, 25.5 ± 0.2% for acetyl- and 52.3 ± 0.8% for butyryl-cholinesterase, respectively, whilst the blends insignificantly inhibited both cholinesterases. The results showed that polyphenol-rich BSG fractions have potentials as natural anti-cholinesterase agents.

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Co-Production of Protein Hydrolysates and 2,3-Butanediol from Brewer’s Spent Grain

ACS Sustainable Chemistry & Engineering ◽

10.1021/acssuschemeng.1c04162 ◽

2021 ◽

Author(s):

Yanhong He ◽

Joshua A. OHair ◽

Qing Jin ◽

Zhiyuan Xu ◽

Jian Wu ◽

...

Keyword(s):

Protein Hydrolysates ◽

Brewer’S Spent Grain ◽

Spent Grain

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Brewer’s spent grain fermentation improves its soluble sugar and protein as well as enzymatic activities using Bacillus velezensis

Process Biochemistry ◽

10.1016/j.procbio.2021.10.016 ◽

2021 ◽

Author(s):

Jiarui Zeng ◽

Wenqi Huang ◽

Xiaofei Tian ◽

Xi Hu ◽

Zhenqiang Wu

Keyword(s):

Soluble Sugar ◽

Enzymatic Activities ◽

Bacillus Velezensis ◽

Brewer’S Spent Grain ◽

Spent Grain

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STUDY OF THE KINETICS OF DRYING BREWER’S SPENT GRAIN

Известия вузов. Пищевая технология ◽

10.26297/0579-3009.2020.4.18 ◽

2020 ◽

Author(s):

Т.Г. КОРОТКОВА ◽

А.С. ДАНИЛЬЧЕНКО ◽

Н.Ю. ИСТОШИНА

Keyword(s):

Matter Content ◽

Dry Matter Content ◽

Drying Time ◽

Brewer’S Spent Grain ◽

Average Value ◽

Free Moisture ◽

Before And After ◽

Spent Grain ◽

Kinetics Of ◽

Two Temperature

Исследована кинетика сушки сырой пивной дробины – вторичного продукта ООО «Белореченский пивоваренный завод» (Россия, Краснодарский край). Сушка проведена в двух температурных режимах – 60 и 55°С. Скорость сушильного агента при вынужденной конвекции составляла 4,5 м/с. Содержание сухого вещества определено по ГОСТ 31640–2012 в лабораторных условиях и составило 12,9%. Измерение убыли массы при сушке проведено с интервалом 5 мин. Общее время сушки навески пивной дробины в количестве 50 г – 420 мин при режиме 50°С, 360 мин – при 60°С. Среднее значение конечной влажности пивной дробины составило 11,85%. Построены кривые сушки и скорости сушки. Скорость сушки при удалении свободной влаги в первом периоде сушки, мин–1: при 60°С – 3,5, при 55°С – 3,0. Установлено, что содержание связанной влаги составляет в среднем 57% от массы влаги в навеске пивной дробины, свободной влаги – 43%. Анализ структуры навески пивной дробины под микроскопом до сушки и после нее подтвердил факт, что в исследованном материале количество связанной влаги больше, чем свободной. Наличие частиц размером 5–7 мм свидетельствует о значительном количестве клеточной влаги в неразрушенных капиллярах. Дальнейшее совершенствование технологии переработки пивной дробины должно быть направлено на разрушение клеточной структуры материала после его механического обезвоживания. The kinetics of drying of the crude brewer’s spent grain – a secondary product of LLC «Belorechensky brewery» (Russian Federation, Krasnodarregion) has been studied. Drying is carried out in two temperature modes – 60 and 55°C. The speed of the drying agent under forced convection was 4,5 m/s. The dry matter content was determined according to GOST 31640–2012 in laboratory conditions and was 12,9%. Measurement of mass loss during drying was performed at an interval of 5 min. The total drying time of the brewer’s spent grain sample in the amount of 50 gis 420 min at 50°C, 360 min – at 60°C. The average value of the final moisture content of brewer’s spent grain made up 11,85%. Curves of drying and drying speed are constructed. Drying speed when removing free moisture in the first drying period, min–1: at 60°C – 3,5, at 55°C – 3,0. It was found that the content on average of bound moisture is 57% of the mass of moisture in brewer’s spent grain, free moisture – 43%. Analysis of the structure sample of brewer’s spent grain a microscope before and after drying confirmed the fact that the amount of bound moisture in the studied material is greater than the amount of free moisture. The presence of particles of 5–7 mm in size indicates a significant amount of cellular moisture in undisturbed capillaries. Further improvement of the technology for processing brewer’s spent grain should be aimed at destroying the cellular structure of the material after its mechanical dehydration.

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Fluid dynamics analysis and pyrolysis of brewer’s spent grain in a spouted bed reactor

Particuology ◽

10.1016/j.partic.2018.06.001 ◽

2019 ◽

Vol 42 ◽

pp. 199-207 ◽

Cited By ~ 7

Author(s):

Marcos A.S. Barrozo ◽

Lidja D.M.S. Borel ◽

Taísa S. Lira ◽

Carlos H. Ataíde

Keyword(s):

Fluid Dynamics ◽

Dynamics Analysis ◽

Spouted Bed ◽

Brewer’S Spent Grain ◽

Spent Grain

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Removal of the Pesticide Pymetrozine from Aqueous Solution by Biochar Produced from Brewer's Spent Grain at Different Pyrolytic Temperatures

BioResources ◽

10.15376/biores.9.4.7696-7709 ◽

2014 ◽

Vol 9 (4) ◽

Cited By ~ 3

Author(s):

Xinguo Xi ◽

Jinlong Yan ◽

Guixiang Quan ◽

Liqiang Cui

Keyword(s):

Aqueous Solution ◽

Brewer’S Spent Grain ◽

Spent Grain

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Development of New Chip Products from Brewer’s Spent Grain

Journal of Food Quality ◽

10.1155/2021/5521746 ◽

2021 ◽

Vol 2021 ◽

pp. 1-6

Author(s):

Reann Garrett ◽

Danielle Bellmer ◽

William McGlynn ◽

Patricia Rayas-Duarte

Keyword(s):

Sensory Evaluation ◽

Rating Scale ◽

Animal Feed ◽

Value Added ◽

Human Consumption ◽

Processing Waste ◽

Brewing Industry ◽

Consumer Responses ◽

Brewer’S Spent Grain ◽

Spent Grain

Brewer’s spent grain (BSG) is a processing waste generated in large quantities by the brewing industry. It is estimated that over 38 million tons of BSG is produced worldwide each year and is usually used as animal feed, composted, or thrown into landfills. BSG contains valuable nutritional components, including protein, fiber, and antioxidants. Due to its brittle texture, strong nutty flavors, and dark color profiles, BSG has seen limited use in food products for human consumption. The objective of this study was to develop a palatable chip product that maximized the level of inclusion of BSG. Chips were produced that contained BSG levels ranging from 8% to 40%, and the physical and sensory properties of the chips were evaluated. Spent grain samples were provided by Iron Monk in Stillwater and were dried at a low temperature and milled into flour for use in the chip formulation. BSG chips were evaluated for water activity, color, and texture (fracture force). An informal sensory evaluation was conducted to evaluate flavor, texture, and probability of purchase using a 5-point hedonic rating scale. Results showed that there were no significant differences in the texture of the chips containing different levels of BSG. The color measurements showed no significant differences between L ∗ and a ∗ values for the chips containing different inclusion levels of BSG, but there were some differences in the b ∗ values. Results from the sensory evaluation showed that consumers preferred the texture of chips with 40% BSG over chips with 8% BSG, and they were also more likely to purchase the 40% BSG chips. There were no significant differences in flavor among the chips containing different inclusion levels of BSG. These results suggest that, for a chip-type product, BSG inclusion levels up to 40% are possible with positive consumer responses. Development of an alternative value-added product represents an opportunity for breweries nationwide to turn a processing waste into a future asset.

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