Co-Production of Protein Hydrolysates and 2,3-Butanediol from Brewer’s Spent Grain

Abstract Background Microbial surfactants called biosurfactants, thanks to their high biodegradability, low toxicity and stability can be used not only in bioremediation and oil processing, but also in the food and cosmetic industries, and even in medicine. However, the high production costs of microbial surfactants and low efficiency limit their large-scale production. This requires optimization of management conditions, including the possibility of using waste as a carbon source, such as food processing by-products. This papers describes the production and characterization of the biosurfactant obtained from the endophytic bacterial strain Bacillus pumilus 2A grown on various by-products of food processing and its potential applications in supporting plant growth. Four different carbon and nitrogen sources, pH, inoculum concentration and temperature were optimized within Taguchi method. Results Optimization of bioprocess within Taguchi method and experimental analysis revealed that the optimal conditions for biosurfactant production were brewer’s spent grain (5% w/v), ammonium nitrate (1% w/v), pH of 6, 5% of inoculum, and temperature at 30 °C, leading to 6.8 g/L of biosurfactant. Based on gas chromatography–mass spectrometry and Fourier transform infrared spectroscopy analysis produced biosurfactant was determined as glycolipid. Obtained biosurfactant has shown high and long term thermostability, surface tension of 47.7 mN/m, oil displacement of 8 cm and the emulsion index of 69.11%. The examined glycolipid, used in a concentration of 0.2% significantly enhanced growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Conclusions The endophytic Bacillus pumilus 2A produce glycolipid biosurfactant with high and long tem thermostability, what makes it useful for many purposes including food processing. The use of brewer’s spent grain as the sole carbon source makes the production of biosurfactants profitable, and from an environmental point of view, it is an environmentally friendly way to remove food processing by products. Glycolipid produced by endophytic Bacillus pumilus 2A significantly improve growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Obtained results provide new insight to the possible use of glycolipids as plant growth promoting agents.

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Potential of Brewer’s Spent Grain as a Potential Replacement of Wood in pMDI, UF or MUF Bonded Particleboard

Polymers ◽

10.3390/polym13030319 ◽

2021 ◽

Vol 13 (3) ◽

pp. 319

Author(s):

Marius Cătălin Barbu ◽

Zeno Montecuccoli ◽

Jakob Förg ◽

Ulrike Barbeck ◽

Petr Klímek ◽

...

Keyword(s):

Internal Bond ◽

Mechanical Performance ◽

Animal Feed ◽

Urea Formaldehyde ◽

Modulus Of Rupture ◽

Brewer’S Spent Grain ◽

Beer Brewing ◽

Removal Of Heavy Metals ◽

Dry Conditions ◽

Spent Grain

Brewer’s spent grain (BSG) is the richest by-product (85%) of the beer-brewing industry, that can be upcycled in a plentiful of applications, from animal feed, bioethanol production or for removal of heavy metals from wastewater. The aim of this research is to investigate the mechanical, physical and structural properties of particleboard manufactured with a mixture of wood particles and BSG gradually added/replacement in 10%, 30% and 50%, glued with polymeric diisocyanate (pMDI), urea-formaldehyde (UF) and melamine urea-formaldehyde (MUF) adhesives. The density, internal bond, modulus of rupture, modulus of elasticity, screw withdrawal resistance, thickness swelling and water absorption were tested. Furthermore, scanning electron microscopy anaylsis was carried out to analyze the structure of the panels after the internal bond test. Overall, it was shown that the adding of BSG decreases the mechanical performance of particleboard, due to reduction of the bonding between wood and BSG particles. This decrease has been associated with the structural differences proven by SEM inspection. Interaction of particles with the adhesive is different for boards containing BSG compared to those made from wood. Nevertheless, decrease in the mechanical properties was not critical for particleboards produced with 10% BSG which could be potentially classified as a P2 type, this means application in non-load-bearing panel for interior use in dry conditions, with high dimensional stability and stiffness.

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Recycling of Brewer’s Spent Grain as a Biosorbent by Nitro-Oxidation for Uranyl Ion Removal from Wastewater

ACS Omega ◽

10.1021/acsomega.1c00589 ◽

2021 ◽

Author(s):

Yi Su ◽

Marco Wenzel ◽

Silvia Paasch ◽

Markus Seifert ◽

Wendelin Böhm ◽

...

Keyword(s):

Uranyl Ion ◽

Brewer’S Spent Grain ◽

Ion Removal ◽

Spent Grain

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Anticholinesterase Activities of Different Solvent Extracts of Brewer’s Spent Grain

Foods ◽

10.3390/foods10050930 ◽

2021 ◽

Vol 10 (5) ◽

pp. 930

Author(s):

Rares I. Birsan ◽

Peter Wilde ◽

Keith W. Waldron ◽

Dilip K. Rai

Keyword(s):

Ethyl Acetate Fraction ◽

Therapeutic Approaches ◽

Total Polyphenol ◽

Brewer’S Spent Grain ◽

Dietary Polyphenols ◽

Synthetic Drugs ◽

Naturally Occurring ◽

Diferulic Acid ◽

Spent Grain ◽

Butyryl Cholinesterase

Cholinesterases, involved in acetylcholine catabolism in the central and peripheral nervous system, have been strongly linked with neurodegenerative diseases. Current therapeutic approaches using synthetic drugs present several side effects. Hence, there is an increasing research interest in naturally-occurring dietary polyphenols, which are also considered efficacious. Food processing by-products such as brewer’s spent grain (BSG) would be a potential bio-source of polyphenols. In this study, polyphenol-rich BSG extracts using 60% acetone and 0.75% NaOH solutions were generated, which were further subjected to liquid–liquid partitioning using various organic solvents. The water-partitioned fractions of the saponified extracts had the highest total polyphenol content (6.2 ± 2.8mgGAE/g dw) as determined by Folin–Ciocalteu reagent, while the LC-MS/MS showed ethyl acetate fraction with the highest phenolics (2.9 ± 0.3mg/g BSG dw). The best inhibitions of acetyl- (37.9 ± 2.9%) and butyryl- (53.6 ± 7.7%) cholinesterases were shown by the diethyl ether fraction of the saponified extract. This fraction contained the highest sum of quantified phenolics (99 ± 21.2µg/mg of extract), and with significant (p < 0.01) inhibitory contribution of decarboxylated-diferulic acid. Amongst the standards, caffeic acid presented the highest inhibition for both cholinesterases, 25.5 ± 0.2% for acetyl- and 52.3 ± 0.8% for butyryl-cholinesterase, respectively, whilst the blends insignificantly inhibited both cholinesterases. The results showed that polyphenol-rich BSG fractions have potentials as natural anti-cholinesterase agents.

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Effects of brewers’ spent grain protein hydrolysates on gas production, ruminal fermentation characteristics, microbial protein synthesis and microbial community in an artificial rumen fed a high grain diet

Journal of Animal Science and Biotechnology ◽

10.1186/s40104-020-00531-5 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Tao Ran ◽

Long Jin ◽

Ranithri Abeynayake ◽

Atef Mohamed Saleem ◽

Xiumin Zhang ◽

...

Keyword(s):

Protein Synthesis ◽

Microbial Community ◽

Antioxidant Properties ◽

Value Added ◽

Gas Production ◽

Protein Hydrolysates ◽

Ruminal Fermentation ◽

Microbial Protein ◽

Microbial Protein Synthesis ◽

Spent Grain

Abstract Background Brewers’ spent grain (BSG) typically contains 20% – 29% crude protein (CP) with high concentrations of glutamine, proline and hydrophobic and non-polar amino acid, making it an ideal material for producing value-added products like bioactive peptides which have antioxidant properties. For this study, protein was extracted from BSG, hydrolyzed with 1% alcalase and flavourzyme, with the generated protein hydrolysates (AlcH and FlaH) showing antioxidant activities. This study evaluated the effects of AlcH and FlaH on gas production, ruminal fermentation characteristics, nutrient disappearance, microbial protein synthesis and microbial community using an artificial rumen system (RUSITEC) fed a high-grain diet. Results As compared to the control of grain only, supplementation of FlaH decreased (P < 0.01) disappearances of dry matter (DM), organic matter (OM), CP and starch, without affecting fibre disappearances; while AlcH had no effect on nutrient disappearance. Neither AlcH nor FlaH affected gas production or VFA profiles, however they increased (P < 0.01) NH3-N and decreased (P < 0.01) H2 production. Supplementation of FlaH decreased (P < 0.01) the percentage of CH4 in total gas and dissolved-CH4 (dCH4) in dissolved gas. Addition of monensin reduced (P < 0.01) disappearance of nutrients, improved fermentation efficiency and reduced CH4 and H2 emissions. Total microbial nitrogen production was decreased (P < 0.05) but the proportion of feed particle associated (FPA) bacteria was increased with FlaH and monensin supplementation. Numbers of OTUs and Shannon diversity indices of FPA microbial community were unaffected by AlcH and FlaH; whereas both indices were reduced (P < 0.05) by monensin. Taxonomic analysis revealed no effect of AlcH and FlaH on the relative abundance (RA) of bacteria at phylum level, whereas monensin reduced (P < 0.05) the RA of Firmicutes and Bacteroidetes and enhanced Proteobacteria. Supplementation of FlaH enhanced (P < 0.05) the RA of genus Prevotella, reduced Selenomonas, Shuttleworthia, Bifidobacterium and Dialister as compared to control; monensin reduced (P < 0.05) RA of genus Prevotella but enhaced Succinivibrio. Conclusions The supplementation of FlaH in high-grain diets may potentially protect CP and starch from ruminal degradation, without adversely affecting fibre degradation and VFA profiles. It also showed promising effects on reducing CH4 production by suppressing H2 production. Protein enzymatic hydrolysates from BSG using flavourzyme showed potential application to high value-added bio-products.

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Brewer’s spent grain fermentation improves its soluble sugar and protein as well as enzymatic activities using Bacillus velezensis

Process Biochemistry ◽

10.1016/j.procbio.2021.10.016 ◽

2021 ◽

Author(s):

Jiarui Zeng ◽

Wenqi Huang ◽

Xiaofei Tian ◽

Xi Hu ◽

Zhenqiang Wu

Keyword(s):

Soluble Sugar ◽

Enzymatic Activities ◽

Bacillus Velezensis ◽

Brewer’S Spent Grain ◽

Spent Grain

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STUDY OF THE KINETICS OF DRYING BREWER’S SPENT GRAIN

Известия вузов. Пищевая технология ◽

10.26297/0579-3009.2020.4.18 ◽

2020 ◽

Author(s):

Т.Г. КОРОТКОВА ◽

А.С. ДАНИЛЬЧЕНКО ◽

Н.Ю. ИСТОШИНА

Keyword(s):

Matter Content ◽

Dry Matter Content ◽

Drying Time ◽

Brewer’S Spent Grain ◽

Average Value ◽

Free Moisture ◽

Before And After ◽

Spent Grain ◽

Kinetics Of ◽

Two Temperature

Исследована кинетика сушки сырой пивной дробины – вторичного продукта ООО «Белореченский пивоваренный завод» (Россия, Краснодарский край). Сушка проведена в двух температурных режимах – 60 и 55°С. Скорость сушильного агента при вынужденной конвекции составляла 4,5 м/с. Содержание сухого вещества определено по ГОСТ 31640–2012 в лабораторных условиях и составило 12,9%. Измерение убыли массы при сушке проведено с интервалом 5 мин. Общее время сушки навески пивной дробины в количестве 50 г – 420 мин при режиме 50°С, 360 мин – при 60°С. Среднее значение конечной влажности пивной дробины составило 11,85%. Построены кривые сушки и скорости сушки. Скорость сушки при удалении свободной влаги в первом периоде сушки, мин–1: при 60°С – 3,5, при 55°С – 3,0. Установлено, что содержание связанной влаги составляет в среднем 57% от массы влаги в навеске пивной дробины, свободной влаги – 43%. Анализ структуры навески пивной дробины под микроскопом до сушки и после нее подтвердил факт, что в исследованном материале количество связанной влаги больше, чем свободной. Наличие частиц размером 5–7 мм свидетельствует о значительном количестве клеточной влаги в неразрушенных капиллярах. Дальнейшее совершенствование технологии переработки пивной дробины должно быть направлено на разрушение клеточной структуры материала после его механического обезвоживания. The kinetics of drying of the crude brewer’s spent grain – a secondary product of LLC «Belorechensky brewery» (Russian Federation, Krasnodarregion) has been studied. Drying is carried out in two temperature modes – 60 and 55°C. The speed of the drying agent under forced convection was 4,5 m/s. The dry matter content was determined according to GOST 31640–2012 in laboratory conditions and was 12,9%. Measurement of mass loss during drying was performed at an interval of 5 min. The total drying time of the brewer’s spent grain sample in the amount of 50 gis 420 min at 50°C, 360 min – at 60°C. The average value of the final moisture content of brewer’s spent grain made up 11,85%. Curves of drying and drying speed are constructed. Drying speed when removing free moisture in the first drying period, min–1: at 60°C – 3,5, at 55°C – 3,0. It was found that the content on average of bound moisture is 57% of the mass of moisture in brewer’s spent grain, free moisture – 43%. Analysis of the structure sample of brewer’s spent grain a microscope before and after drying confirmed the fact that the amount of bound moisture in the studied material is greater than the amount of free moisture. The presence of particles of 5–7 mm in size indicates a significant amount of cellular moisture in undisturbed capillaries. Further improvement of the technology for processing brewer’s spent grain should be aimed at destroying the cellular structure of the material after its mechanical dehydration.

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