Substituted Benzimidazole Analogues as Potential α-Amylase Inhibitors and Radical Scavengers

Pregnancy is a physiological state accompanied by a high energy demand of many bodily functions and an increased oxygen requirement. Because of the increased intake and utilization of oxygen, increased levels of oxidative stress would be expected. In the present study, the degree of lipid peroxidation was examined in different tissues from non-pregnant and pregnant rats after the delivery of their young. Melatonin and other indole metabolites are known to be direct free radical scavengers and indirect antioxidants. Thus the effect of pinealectomy at 1 month before pregnancy on the accumulation of lipid damage was investigated in non-pregnant and pregnant rats after the delivery of their young. Malonaldehyde and 4-hydroxyalkenal concentrations were measured in the lung, uterus, liver, brain, kidney, thymus and spleen from intact and pinealectomized pregnant rats soon after birth of their young and at 14 and 21 days after delivery. The same parameters were also evaluated in intact and pinealectomized non-pregnant rats. Shortly after delivery, lipid oxidative damage was increased in lung, uterus, brain, kidney and thymus of the mothers. No differences were detected in liver and spleen. Pinealectomy enhanced this effect in the uterus and lung. It is concluded that during pregnancy high levels of oxidative stress induce an increase in oxidative damage to lipids, which in some cases is inhibited by the antioxidative actions of pineal indoles.

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Nutritional aspects and their influences on the pathophysiology of

Revista de Ciências Médicas ◽

10.24220/2318-0897v23n1a2413 ◽

2014 ◽

Vol 23 (1) ◽

pp. 33

Author(s):

Nathalia Liberato Nascimento ◽

Iwyson Henrique Fernandes da Costa ◽

Rivelilson Mendes de Freitas

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Fatty Acids ◽

Folic Acid ◽

Saturated Fatty Acids ◽

Free Radical Scavengers ◽

Radical Scavengers ◽

High Intake ◽

Neuroprotective Effects ◽

Nutritional Factors

The objective of this study was to conduct a review about the nutritional aspects and their influences on the pathophysiology of Alzheimer’s disease. The review describes the pathophysiology of Alzheimer’s disease, the generally indicated diets, and the nutritional factors that may aggravate the disease based on a literature review using the following keywords in English and Portuguese: “Alzheimer’s disease”, “physiopathology”, “nutritional aspects”, and “antioxidants”. A total of 100 articles were found, 48 in Lilacs and 52 in MedLine, but only 54 articles were selected for the review. The use of antioxidants as free radical scavengers is generally indicated in diets for Alzheimer’s patients. Studies also suggest that caffeine, vitamin B12, and folic acid have neuroprotective effects. Cohort studies found that a high intake of saturated fatty acids and obesity increase the risk of Alzheimer’s disease. People with Alzheimer’s disease should avoid diets high in carbohydrates and saturated fats, and prefer foods high in antioxidants.Keywords: Alzheimer disease; Antioxidants; Neurophysiology; Review literture as topic.

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ISOLATION of a AMYLASE INHIBITORS from MUNGBEAN and SOYBEAN and INHIBITORY EFFECT on HUMAN SALIVARY and PORCINE PANCREATIC AMYLASE

Jurnal Sains dan Teknologi Indonesia ◽

10.29122/jsti.v14i1.899 ◽

2013 ◽

Vol 14 (1) ◽

Author(s):

Budiasih Wahyuntari ◽

Martinus Nicoadi Tekol Tekol

Keyword(s):

Inhibitory Effect ◽

Pancreatic Amylase ◽

Sds Page ◽

Amylase Inhibitors

Penghambat alfa amilase adalah salah satu komponen dalam suplemen makanan yang telah lama digunakan untuk terapi kegemukan karena penghambat amilase mempengaruhi metabolisme karbohidrat dalam sistem pencernaan. Sejumlah peneliti melaporkan terdapat dua grup penghambat amilase, yaitu protein dan non protein. Penghambat protein dilaporkan terdapat dalam kelompok kacang-kacangan dan biji-bijian. Tujuan penelitian ini adalah untuk mengisolasi penghambat protein yang terdapat dalam kacang hijau dan kedele. Kacang hijau dan kedele merupakan kacang-kacangan yang penting dalam makanan popular di Indonesia. Penghambat protein diendapkan dengan konsentrasi bertingkat garam ammonium sulfat [(NH)4SO4] dari 30-70%. Penghambat protein diuji terhadap amilase saliva manusia (HSA) danamilase pankreas babi (PPA), serta kestabilannya terhadap pemanasan pada 100oC selama 30 menit. Hasil penelitian menunjukkan bahwa semua endapan jenuh dari semua konsentrasi amonium sulfat yang diuji menghambat PPA, tetapi tidak semua endapan jenuh tersebut dapatmenghambat HSA. Hanya semua endapan jenuh (NH)4SO4 dari kacang hijau yang dapat menghambat HSA, dan penghambatan tertinggi terhadap HSA adalah endapan jenuh (NH)4SO4 50%. Endapan jenuh (NH)4SO4 40 % dari kedele putih dan endapan jenuh (NH)4SO4 60% dari kedele hitam dengan masing-masing penghambatan 98.67; 26.86 and27.63%. Endapan jenuh (NH)4SO4 60-70% dari kacang hijau, 50% dari kedele putih dan 50% dari kedele hitam menghambat PPA 100%. Pemanasan penghambat pada 100oC selama 30 menit hampir tidak mempengaruhi penghambatannya terhadap PPA. Profil protein juga diamati menggunakan analisis SDS/PAGE.

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Screening of Actinomycetes For α-amylase Inhibitors Production

Current Enzyme Inhibition ◽

10.2174/1573408015666190110130544 ◽

2019 ◽

Vol 15 (1) ◽

pp. 41-45

Author(s):

Shivabai Chandwad ◽

Sudhakar Gutte

Keyword(s):

Inhibitory Activity ◽

Enzyme Inhibitors ◽

Spectroscopic Method ◽

Solid Material ◽

Soil Samples ◽

Assay Method ◽

Novel Drugs ◽

Pre Treatment ◽

Diabetic Treatment ◽

Amylase Inhibitors

Background:Diabetes mellitus is the most common and fastest growing disease in the world. One of the therapies to treat diabetes is the inhibition of α-amylase activity by inhibitors from microbial and plant source. Actinomycetes are potential sources of enzyme inhibitors, drugs, amino acids, vitamins etc.Objective:Our work mainly highlights the isolation of actinomycetes from soil samples of different habitats and screening of α -amylase inhibitors.Methods:Actinomycetes were isolated from soil samples of different habitats by different methods; these include a variety of pre-treatment of soil samples in combination with an appropriate supplement medium with selective antibacterial agents. Isolated actinomycetes grown in fermentation condition and metabolites were extracted with Isopropyl alcohol and concentrated to obtain solid material. The extract of each isolate was tested for α -amylase inhibition using starch Iodine plate method and DNS- spectroscopic method.Results:Total 110 actinomycetes strains were isolated from various sources. Among 110 extracts of actinomycetes, eight extracts have shown positive results for α-amylase inhibition in starch Iodine plate assay method. Extracts selected from primary results were used for the confirmation of inhibitory activity using DNS- spectroscopic method. Out of eight extracts, six extracts showed Porcine pancreatic α -amylase inhibitory activity ranging from 40-86%. The actinomycetes strains that produce α -amylase inhibitory activity are A-24, A-29, B-5, B-18, C-15 and D-24.Conclusion:These results show that actinomycetes are a potential source for α -amylase inhibitors, which may lead to valuable novel drugs for diabetic treatment.

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Oxidative hemolysis of erythrocytes and its inhibition by free radical scavengers.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)77707-2 ◽

1988 ◽

Vol 263 (36) ◽

pp. 19809-19814

Author(s):

E Niki ◽

E Komuro ◽

M Takahashi ◽

S Urano ◽

E Ito ◽

...

Keyword(s):

Free Radical ◽

Free Radical Scavengers ◽

Radical Scavengers ◽

Oxidative Hemolysis

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Structure-based design of human pancreatic amylase inhibitors from the natural anthocyanin database for type 2 diabetes

Food & Function ◽

10.1039/c9fo02885d ◽

2020 ◽

Vol 11 (4) ◽

pp. 2910-2923 ◽

Cited By ~ 3

Author(s):

Lianghua Xie ◽

Jianling Mo ◽

Jingdan Ni ◽

Yang Xu ◽

Hongming Su ◽

...

Keyword(s):

Type 2 Diabetes ◽

Design Approach ◽

Pancreatic Amylase ◽

Amylase Inhibitor ◽

Amylase Inhibitors

Malvidin 3-O-arabinoside is identified as a novel human pancreatic amylase inhibitor from the natural anthocyanin database with a structure-based design approach.

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