Prediction of Gene Expression Levels and the Role of Cis-Acting Elements in Age-Related Cataract by Applying a Promoter-Based Modeling Approach

Introduction: Gene mutation is an infrequent cause of tumor suppressor gene (TSG) defect in de novo AML patients. Instead, it seems that leukemic cells employ epigenetic tricks to attenuate the negative impacts of intact TSGs. Ordinarily, critical TSGs, such as p16INK4A, is hyper-methylated in AML blasts under the impact of master epigenetic regulators, such as UHRF1. In this study, we investigated the correlation between UHRF1 and p16INK4A gene expression levels in newly diagnosed AML patients. Methods: Bone marrow and peripheral blood samples were obtained from 50 newly diagnosed AML patients and 18 healthy normal control subjects. Gene expression levels of UHRF1 and P16INK4A were surveyed using SYBR Green Quantitative Real-time PCR. Statistical analyses were done using SPSS statistical software 21.0. Results: P16INK4A gene expression showed reduced levels in 80.64% of patients above 45 years of age, while only 32% of patients below 45 years had reduced expression levels. The Spearman correlation test also demonstrated a significant negative correlation between UHRF1 and p16INK4A gene expression levels in AML patients, which was not observed in the control group (r=0.343 and P= 0.015). Conclusion: Regarding the age-related patterns of UHRF1 and p16INK4A gene expression, and also the presence of negative correlation between them, we conclude that UHRF1 may potentially be involved in p16INK4A down-regulation in elderly AML patients, which may subsequently facilitate the progression of AML in older ages.

Download Full-text

Gene expression patterns are correlated with genomic and genic structure in soybean

Genome ◽

10.1139/g10-090 ◽

2011 ◽

Vol 54 (1) ◽

pp. 10-18 ◽

Cited By ~ 15

Author(s):

Jenna L. Woody ◽

Andrew J. Severin ◽

Yung-Tsi Bolon ◽

Bindu Joseph ◽

Brian W. Diers ◽

...

Keyword(s):

Gene Expression ◽

Sequence Data ◽

Expression Patterns ◽

Nucleotide Composition ◽

Physical Parameters ◽

Expression Levels ◽

Noncoding Regions ◽

Expression Breadth ◽

Gene Expression Levels

Studies have indicated that exon and intron size and intergenic distance are correlated with gene expression levels and expression breadth. Previous reports on these correlations in plants and animals have been conflicting. In this study, next-generation sequence data, which has been shown to be more sensitive than previous expression profiling technologies, were generated and analyzed from 14 tissues. Our results revealed a novel dichotomy. At the low expression level, an increase in expression breadth correlated with an increase in transcript size because of an increase in the number of exons and introns. No significant changes in intron or exon sizes were noted. Conversely, genes expressed at the intermediate to high expression levels displayed a decrease in transcript size as their expression breadth increased. This was due to smaller exons, with no significant change in the number of exons. Taking advantage of the known gene space of soybean, we evaluated the positioning of genes and found significant clustering of similarly expressed genes. Identifying the correlations between the physical parameters of individual genes could lead to uncovering the role of regulation owing to nucleotide composition, which might have potential impacts in discerning the role of the noncoding regions.

Download Full-text

Low Zip 4 gene expression levels in RPMI - 8226 and ARH - 77 cell lines support the possible role of zip 4 transporter protein in plasma cell tumorogenesis

Cumhuriyet Medical Journal ◽

10.7197/1305-0028.1705 ◽

2013 ◽

Vol 35 (1) ◽

pp. 9-13

Author(s):

Zehra Dilşad Çoban ◽

Deniz Torun ◽

Ferit Avcu ◽

Ali Uğur Ural ◽

Erhan Parıltay ◽

...

Keyword(s):

Gene Expression ◽

Cell Lines ◽

Plasma Cell ◽

Expression Levels ◽

Transporter Protein ◽

Rpmi 8226 ◽

Gene Expression Levels

Download Full-text

The Effect of Eight Weeks of Training on Positive and Negative Slopes with Royal Jelly Consumption on MGMT and ATPase Gene Expression Levels in the Hippocampus Tissue of Trimethyltin-Induced Alzheimer’s Rats

Gene Cell and Tissue ◽

10.5812/gct.113871 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Zahra Giti ◽

Abdolali Banaeifar ◽

Sajad Arshadi ◽

Mohammad Ali Azarbayjani

Keyword(s):

Gene Expression ◽

Royal Jelly ◽

Interactive Effect ◽

Negative Slope ◽

Common Disease ◽

Positive Slope ◽

Expression Levels ◽

Atpase Gene ◽

Gene Expression Levels

Background: Alzheimer’s disease (AD) is a common disease in the elderly that is associated with impaired metabolism and biology of the hippocampus. Although the role of exercise and natural antioxidants in improving the disease has been reported, the role of muscle contraction-related physical activity along with royal jelly (RJ) consumption is not yet well understood. Objectives: This study aimed to investigate the effect of eight weeks of training on positive slope (ETPS) and negative slope (ETNS) with royal jelly (RJ) consumption on O-6-methylguanine DNA methyltransferase (MGMT) and ATPase gene expression levels in the hippocampus tissue of trimethyltin (TMT)-induced AD rats. Methods: In this experimental trial, 36 male Sprague-Dawley AD rats (induced with 8 mg/kg TMT) were divided into (1) control + normal saline/royal jelly solvent) (Sh); (2) ETPS; (3) ETNS; (4) ETPS + RJ; (5) ETNS + RJ, and (6) RJ groups. Six rats were placed in the healthy control (HC) group. Then the training groups trained on ( + 15 and -15) slopes for eight weeks, five sessions per week, and each session lasted 60 minutes at a speed of 16 m/min. The royal jelly (RJ) groups received 100 mg/kg royal jelly per day by intraperitoneal injection. Results: ETNS, ETPS, ETPS + RJ, and ETNS + RJ increased MGMT gene expression (P ≥ 0.05). ETPS and ETPS + RJ also increased ATPase gene expression (P ≥ 0.05). However, RJ had no significant effect on increasing MGMT and ATPase gene expression in the hippocampus tissue of AD rats (P ≥ 0.05). Conclusions: It seems that the improvement of DNA damage markers and energy levels depends on the type of contraction. Although training on positive and negative slopes with royal jelly consumption has an interactive effect on DNA repair markers, training on a positive slope and royal jelly consumption has an interactive effect on ATPase gene expression.

Download Full-text

IL-10 deficiency protects hamsters from Leptospira infection

Infection and Immunity ◽

10.1128/iai.00584-21 ◽

2021 ◽

Author(s):

Xufeng Xie ◽

Tianbao Lv ◽

Dianjun Wu ◽

Haozhe Shi ◽

Shilei Zhang ◽

...

Keyword(s):

Gene Expression ◽

Survival Rate ◽

Zoonotic Disease ◽

Protective Effects ◽

Subclinical Infection ◽

Expression Levels ◽

Tnf Α ◽

Gene Expression Levels

Leptospirosis is a globally spread zoonotic disease with outcomes ranging from subclinical infection to fatal Weil syndrome. In addition to antibiotics, some immune activators have shown protective effects against leptospirosis. However, the unclear relationship between Leptospira and cytokines, has limited the development of antileptospiral immunomodulators. In this study, the particular role of IL-10 in leptospirosis was explored by using IL-10 defective (IL-10 -/- ) hamsters. After Leptospira infection, an improved survival rate, reduced leptospiral burden and alleviation of organ lesions were found in IL-10 -/- hamsters compared with WT hamsters. In addition, the gene expression levels of IL-1β, IL-6 and TNF-α and the NO level were higher in IL-10 -/- hamsters than in WT hamsters. Our results indicate that IL-10 deficiency protects hamsters from Leptospira infection.

Download Full-text

Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors

Journal of Clinical Medicine ◽

10.3390/jcm9061838 ◽

2020 ◽

Vol 9 (6) ◽

pp. 1838

Author(s):

Araceli García-Martínez ◽

Antonio C. Fuentes-Fayos ◽

Carmen Fajardo ◽

Cristina Lamas ◽

Rosa Cámara ◽

...

Keyword(s):

Gene Expression ◽

Transcription Factor ◽

Transcription Factors ◽

Potential Role ◽

Therapeutic Option ◽

Pharmacological Treatments ◽

Expression Levels ◽

Qrt Pcr ◽

Gene Expression Levels

The potential role of miRNAs in the silencing mechanisms of pituitary neuroendocrine tumors (PitNETs) has not been addressed. The aim of the present study was to evaluate the expression levels and the potential associated role of some miRNAs, pathways, and transcription factors in the silencing mechanisms of corticotroph tumors (CTs). Accordingly, the expression of miR-375, miR-383, miR-488, miR-200a and miR-103; of PKA, MAP3K8, MEK, MAPK3, NGFIB, NURR1, PITX1, and STAT3 were analyzed via qRT-PCR in 23 silent and 24 functioning CTs. miR-200a and miR-103 showed significantly higher expression in silent than in functioning CTs, even after eliminating the bias of tumor size, therefore enabling the differentiation between the two variants. Additionally, miR-383 correlated negatively with TBX19 in silent CTs, a transcription factor related with the processing of POMC that can participate in the silencing mechanisms of CTs. Finally, the gene expression levels of miR-488, miR-200a, and miR-103 were significantly higher in macroadenomas (functioning and silent) than in microadenomas. The evidence from this study indicates that miRNAs could be involved in the pathophysiology of CTs. The translational implications of these findings suggest that pharmacological treatments specifically targeting these miRNAs could become a promising therapeutic option for these patients.

Download Full-text

Association of the Adipokines Chemerin, Apelin, Vaspin and Omentin and Their Functional Genetic Variants with Rheumatoid Arthritis

Journal of Personalized Medicine ◽

10.3390/jpm11100976 ◽

2021 ◽

Vol 11 (10) ◽

pp. 976

Author(s):

Alaa S. Wahba ◽

Maha E. Ibrahim ◽

Dina M. Abo-elmatty ◽

Eman T. Mehanna

Keyword(s):

Rheumatoid Arthritis ◽

Gene Expression ◽

Genetic Variants ◽

Serum Levels ◽

Expression Levels ◽

Protein Levels ◽

Egyptian Patients ◽

Gene Expression Levels ◽

Increased Susceptibility

Adipokines were shown to exert crucial roles in rheumatic diseases. This study aimed to assess the role of chemerin, apelin, vaspin, and omentin adipokines and their genetic variants rs17173608, rs2235306, rs2236242, and rs2274907, respectively, in rheumatoid arthritis (RA) pathogenesis in Egyptian patients. A total of 150 RA patients and 150 healthy individuals were recruited. Blood samples were collected and used for genotyping. Serum was separated and used for expression analysis by quantitative PCR, and various biochemical markers determination by ELISA. Serum protein levels of chemerin and vaspin, as well as their gene expression levels were higher, while those of apelin and omentin were lower in RA patients and were associated with most of RA clinical and laboratory characteristics. G allele of chemerin rs17173608, T allele of vaspin rs2236242, and T allele of omentin rs2274907 were more frequent in RA patients. Serum levels and gene expression levels of chemerin in GG genotype carriers and vaspin in TT genotype group were significantly higher, while those of omentin in TT genotype carriers were significantly lower than RA patients with other genotypes. There was no association between apelin rs2235306 and RA. Chemerin rs17173608, vaspin rs2236242, and omentin rs2274907 polymorphisms were associated with increased susceptibility to RA.

Download Full-text

Role of matrix metalloproteinase-7 and apoptosis-associated gene expression levels in the pathogenesis of atrial fibrosis in a Beagle dog model

Molecular Medicine Reports ◽

10.3892/mmr.2017.7415 ◽

2017 ◽

Vol 16 (5) ◽

pp. 6967-6973 ◽

Cited By ~ 1

Author(s):

Min Jia ◽

Zai-Bo Li ◽

Lin Li ◽

Hong-Tao Chu ◽

Zhuang-Zhuang Li

Keyword(s):

Gene Expression ◽

Matrix Metalloproteinase ◽

Atrial Fibrosis ◽

Expression Levels ◽

Beagle Dog ◽

Dog Model ◽

Matrix Metalloproteinase 7 ◽

Gene Expression Levels

Download Full-text

Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting

Genes ◽

10.3390/genes12111767 ◽

2021 ◽

Vol 12 (11) ◽

pp. 1767

Author(s):

Tongyu Zhang ◽

Yu Chen ◽

Junhui Wen ◽

Yaxiong Jia ◽

Liang Wang ◽

...

Keyword(s):

Gene Expression ◽

Elderly Women ◽

Genetic Model ◽

Recovery Period ◽

Reproductive Function ◽

Cell Aging ◽

Expression Levels ◽

Variation Trends ◽

Gene Expression Levels

Molting in birds provides us with an ideal genetic model for understanding aging and rejuvenation since birds present younger characteristics for reproduction and appearance after molting. Forced molting (FM) by fasting in chickens causes aging of their reproductive system and then promotes cell redevelopment by providing water and feed again. To reveal the genetic mechanism of rejuvenation, we detected blood hormone indexes and gene expression levels in the hypothalamus and ovary of hens from five different periods during FM. Three hormones were identified as participating in FM. Furthermore, the variation trends of gene expression levels in the hypothalamus and ovary at five different stages were found to be basically similar using transcriptome analysis. Among them, 45 genes were found to regulate cell aging during fasting stress and 12 genes were found to promote cell development during the recovery period in the hypothalamus. In addition, five hub genes (INO80D, HELZ, AGO4, ROCK2, and RFX7) were identified by WGCNA. FM can restart the reproductive function of aged hens by regulating expression levels of genes associated with aging and development. Our study not only enriches the theoretical basis of FM but also provides insights for the study of antiaging in humans and the conception mechanism in elderly women.

Download Full-text