IL-10 deficiency protects hamsters from Leptospira infection

2021 ◽  
Author(s):  
Xufeng Xie ◽  
Tianbao Lv ◽  
Dianjun Wu ◽  
Haozhe Shi ◽  
Shilei Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Survival Rate ◽  
Zoonotic Disease ◽  
Protective Effects ◽  
Subclinical Infection ◽  
Expression Levels ◽  
Tnf Α ◽  
Gene Expression Levels

Leptospirosis is a globally spread zoonotic disease with outcomes ranging from subclinical infection to fatal Weil syndrome. In addition to antibiotics, some immune activators have shown protective effects against leptospirosis. However, the unclear relationship between Leptospira and cytokines, has limited the development of antileptospiral immunomodulators. In this study, the particular role of IL-10 in leptospirosis was explored by using IL-10 defective (IL-10 -/- ) hamsters. After Leptospira infection, an improved survival rate, reduced leptospiral burden and alleviation of organ lesions were found in IL-10 -/- hamsters compared with WT hamsters. In addition, the gene expression levels of IL-1β, IL-6 and TNF-α and the NO level were higher in IL-10 -/- hamsters than in WT hamsters. Our results indicate that IL-10 deficiency protects hamsters from Leptospira infection.

scholarly journals Gene expression patterns are correlated with genomic and genic structure in soybean

Genome ◽  
2011 ◽  
Vol 54 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Jenna L. Woody ◽  
Andrew J. Severin ◽  
Yung-Tsi Bolon ◽  
Bindu Joseph ◽  
Brian W. Diers ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sequence Data ◽  
Expression Patterns ◽  
Nucleotide Composition ◽  
Physical Parameters ◽  
Expression Levels ◽  
Noncoding Regions ◽  
Expression Breadth ◽  
Gene Expression Levels

Studies have indicated that exon and intron size and intergenic distance are correlated with gene expression levels and expression breadth. Previous reports on these correlations in plants and animals have been conflicting. In this study, next-generation sequence data, which has been shown to be more sensitive than previous expression profiling technologies, were generated and analyzed from 14 tissues. Our results revealed a novel dichotomy. At the low expression level, an increase in expression breadth correlated with an increase in transcript size because of an increase in the number of exons and introns. No significant changes in intron or exon sizes were noted. Conversely, genes expressed at the intermediate to high expression levels displayed a decrease in transcript size as their expression breadth increased. This was due to smaller exons, with no significant change in the number of exons. Taking advantage of the known gene space of soybean, we evaluated the positioning of genes and found significant clustering of similarly expressed genes. Identifying the correlations between the physical parameters of individual genes could lead to uncovering the role of regulation owing to nucleotide composition, which might have potential impacts in discerning the role of the noncoding regions.

scholarly journals Low Zip 4 gene expression levels in RPMI - 8226 and ARH - 77 cell lines support the possible role of zip 4 transporter protein in plasma cell tumorogenesis

2013 ◽  
Vol 35 (1) ◽  
pp. 9-13
Author(s):  
Zehra Dilşad Çoban ◽  
Deniz Torun ◽  
Ferit Avcu ◽  
Ali Uğur Ural ◽  
Erhan Parıltay ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Plasma Cell ◽  
Expression Levels ◽  
Transporter Protein ◽  
Rpmi 8226 ◽  
Gene Expression Levels

scholarly journals The Effect of Eight Weeks of Training on Positive and Negative Slopes with Royal Jelly Consumption on MGMT and ATPase Gene Expression Levels in the Hippocampus Tissue of Trimethyltin-Induced Alzheimer’s Rats

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Zahra Giti ◽  
Abdolali Banaeifar ◽  
Sajad Arshadi ◽  
Mohammad Ali Azarbayjani
Keyword(s):  
Gene Expression ◽  
Royal Jelly ◽  
Interactive Effect ◽  
Negative Slope ◽  
Common Disease ◽  
Positive Slope ◽  
Expression Levels ◽  
Atpase Gene ◽  
Gene Expression Levels

Background: Alzheimer’s disease (AD) is a common disease in the elderly that is associated with impaired metabolism and biology of the hippocampus. Although the role of exercise and natural antioxidants in improving the disease has been reported, the role of muscle contraction-related physical activity along with royal jelly (RJ) consumption is not yet well understood. Objectives: This study aimed to investigate the effect of eight weeks of training on positive slope (ETPS) and negative slope (ETNS) with royal jelly (RJ) consumption on O-6-methylguanine DNA methyltransferase (MGMT) and ATPase gene expression levels in the hippocampus tissue of trimethyltin (TMT)-induced AD rats. Methods: In this experimental trial, 36 male Sprague-Dawley AD rats (induced with 8 mg/kg TMT) were divided into (1) control + normal saline/royal jelly solvent) (Sh); (2) ETPS; (3) ETNS; (4) ETPS + RJ; (5) ETNS + RJ, and (6) RJ groups. Six rats were placed in the healthy control (HC) group. Then the training groups trained on ( + 15 and -15) slopes for eight weeks, five sessions per week, and each session lasted 60 minutes at a speed of 16 m/min. The royal jelly (RJ) groups received 100 mg/kg royal jelly per day by intraperitoneal injection. Results: ETNS, ETPS, ETPS + RJ, and ETNS + RJ increased MGMT gene expression (P ≥ 0.05). ETPS and ETPS + RJ also increased ATPase gene expression (P ≥ 0.05). However, RJ had no significant effect on increasing MGMT and ATPase gene expression in the hippocampus tissue of AD rats (P ≥ 0.05). Conclusions: It seems that the improvement of DNA damage markers and energy levels depends on the type of contraction. Although training on positive and negative slopes with royal jelly consumption has an interactive effect on DNA repair markers, training on a positive slope and royal jelly consumption has an interactive effect on ATPase gene expression.

scholarly journals Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors

2020 ◽  
Vol 9 (6) ◽  
pp. 1838
Author(s):  
Araceli García-Martínez ◽  
Antonio C. Fuentes-Fayos ◽  
Carmen Fajardo ◽  
Cristina Lamas ◽  
Rosa Cámara ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Potential Role ◽  
Therapeutic Option ◽  
Pharmacological Treatments ◽  
Expression Levels ◽  
Qrt Pcr ◽  
Gene Expression Levels

The potential role of miRNAs in the silencing mechanisms of pituitary neuroendocrine tumors (PitNETs) has not been addressed. The aim of the present study was to evaluate the expression levels and the potential associated role of some miRNAs, pathways, and transcription factors in the silencing mechanisms of corticotroph tumors (CTs). Accordingly, the expression of miR-375, miR-383, miR-488, miR-200a and miR-103; of PKA, MAP3K8, MEK, MAPK3, NGFIB, NURR1, PITX1, and STAT3 were analyzed via qRT-PCR in 23 silent and 24 functioning CTs. miR-200a and miR-103 showed significantly higher expression in silent than in functioning CTs, even after eliminating the bias of tumor size, therefore enabling the differentiation between the two variants. Additionally, miR-383 correlated negatively with TBX19 in silent CTs, a transcription factor related with the processing of POMC that can participate in the silencing mechanisms of CTs. Finally, the gene expression levels of miR-488, miR-200a, and miR-103 were significantly higher in macroadenomas (functioning and silent) than in microadenomas. The evidence from this study indicates that miRNAs could be involved in the pathophysiology of CTs. The translational implications of these findings suggest that pharmacological treatments specifically targeting these miRNAs could become a promising therapeutic option for these patients.

scholarly journals Protective effects of aerobic exercise on survival, MOSD, and blood glucose response during sepsis

2021 ◽  
Author(s):  
Xishuai Wang ◽  
Zhiqing Wang ◽  
Donghui Tang
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Aerobic Exercise ◽  
Pulmonary Edema ◽  
Glucose Homeostasis ◽  
Survival Rates ◽  
Expression Levels ◽  
Cell Counts ◽  
Tnf Α ◽  
Gene Expression Levels

Abstract Aim: In the present study, we attempted to investigate whether aerobic exercise could prevent sepsis and its complications and explored the related mechanisms. Methods: Forty ICR mice were divided into four groups: control (Con), LPS, exercise (Ex), and exercise + LPS (Ex + LPS). Low-intensity aerobic exercise was performed for 4 weeks. Forty-eight hours after the last exercise intervention, LPS and Ex+LPS mice received 5 mg/kg LPS intraperitoneally for induction of sepsis. This study examined the effects of a 4-week exercise pretreatment on LPS-induced changes in blood glucose (BG) levels; inflammatory cytokine levels in BALF; bronchoalveolar lavage fluid (BALF) cell counts; the degree of pulmonary edema; neutrophil density in liver, lung, and kidney tissues; and the gene expression levels of IL-1RN, IL-8, IL-10, TNF-α, Sirt-1, and Nrf-2 in lung tissue. Sixty mice were used to perform survival rate analysis. Results: A 4-week exercise pretreatment significantly reversed the histopathological severity of LPS-induced lung, heart, liver, kidney, and aortic injuries (P < 0.05) and ameliorated neutrophil inflammation in the lung, heart, liver, and kidney (P < 0.05). A 4-week exercise pretreatment improved survival rates (P < 0.01), deranged glucose homeostasis (P < 0.01), and pulmonary edema (P < 0.01) in mice with sepsis. A 4-week exercise pretreatment increased the levels of IL-6 (P < 0.05), IL-10 (P < 0.05), and IL-1RN (P < 0.05) and decreased the levels of IL-8 (P < 0.05) and TNF-α (P < 0.05) in BALF. A 4-week exercise pretreatment decreased the gene expression levels of IL-8 (P < 0.01) and TNF-α (P < 0.01) and increased the gene expression levels of IL-1RN (P < 0.01) and IL-10 (P < 0.01) in mice with sepsis. 4-week exercise pretreatment activated the gene expression levels of lung protective factors Sirt-1 (P < 0.01) and Nrf-2 (P < 0.01). Conclusion: Aerobic exercise improved survival rates, multiple organ dysfunction syndromes (MODS), and deranged glucose homeostasis in mice with sepsis. Aerobic exercise alleviated lung injury partly because aerobic exercise exerted immune effects and activated Sirt-1/Nrf-2 signaling.

scholarly journals Association of the Adipokines Chemerin, Apelin, Vaspin and Omentin and Their Functional Genetic Variants with Rheumatoid Arthritis

2021 ◽  
Vol 11 (10) ◽  
pp. 976
Author(s):  
Alaa S. Wahba ◽  
Maha E. Ibrahim ◽  
Dina M. Abo-elmatty ◽  
Eman T. Mehanna
Keyword(s):  
Rheumatoid Arthritis ◽  
Gene Expression ◽  
Genetic Variants ◽  
Serum Levels ◽  
Expression Levels ◽  
Protein Levels ◽  
Egyptian Patients ◽  
Gene Expression Levels ◽  
Increased Susceptibility

Adipokines were shown to exert crucial roles in rheumatic diseases. This study aimed to assess the role of chemerin, apelin, vaspin, and omentin adipokines and their genetic variants rs17173608, rs2235306, rs2236242, and rs2274907, respectively, in rheumatoid arthritis (RA) pathogenesis in Egyptian patients. A total of 150 RA patients and 150 healthy individuals were recruited. Blood samples were collected and used for genotyping. Serum was separated and used for expression analysis by quantitative PCR, and various biochemical markers determination by ELISA. Serum protein levels of chemerin and vaspin, as well as their gene expression levels were higher, while those of apelin and omentin were lower in RA patients and were associated with most of RA clinical and laboratory characteristics. G allele of chemerin rs17173608, T allele of vaspin rs2236242, and T allele of omentin rs2274907 were more frequent in RA patients. Serum levels and gene expression levels of chemerin in GG genotype carriers and vaspin in TT genotype group were significantly higher, while those of omentin in TT genotype carriers were significantly lower than RA patients with other genotypes. There was no association between apelin rs2235306 and RA. Chemerin rs17173608, vaspin rs2236242, and omentin rs2274907 polymorphisms were associated with increased susceptibility to RA.

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