TICT fluorescence emission dependence on excitation wavelength for ethyl p-(dimethylamino)benzoate in supercritical trifluoromethane

Abstract Steady-state fluorescence emission spectra of various celluloses were measured at an excitation wavelength of 320 nm. Various spectra recorded in the solid state were compared: (1) ECF bleached papers made of hardwood, the anhydroglucose units of which were chemically modified at C1 and C6 or C2 and C3 positions with carboxylic groups; (2) microcrystalline cellulose; (3) cotton linters; and (4) delignified sisal fibers (mercerized or not). Fluorescence emission was quite independent of the carboxylic acid content and average molecular weight (determined by viscosimetry) of the cellulose polymers. Microcrystalline cellulose (Avicel), cotton linters, and mercerized delignified sisal cellulose were acetylated in homogeneous medium (DMAc/LiCl as solvent system) to obtain soluble polymers in dichloromethane for comparison of spectra recorded in the solid and liquid states. Fluorescence of cellulose acetates in solution (CH2Cl2) and in the solid state was compared under similar experimental conditions to non-esterified celluloses in the solid state. The importance of the solid state for fluorescence emission could be demonstrated. Fluorophores are present in minute amounts in the polymer and their favorable energy transfer for excitation in the solid state likely enhances fluorescence emission. Among numerous fluorophores, dityrosine appeared to be a good candidate for fluorescence because it displayed emission in the fluorescence range of cellulose. Dityrosine is an amino acid involved in the lignification of non-woody plants. Mercerized sisal impregnated with tyrosine in the presence of peroxidase and hydrogen peroxide did not show enhanced emission, in contrast to para-hydroxycinnamic acid (coumaric acid), which is also involved in the lignification process at least for non-woody plants. The origin of cellulose fluorescence remains uncertain and appears to have several origins. This study clearly underlines the importance of the solid state for enhancing fluorophore emission.

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L-Histidine Stabilized Copper Nanoclusters as a Fluorescence Probe for Determination of Fluazinam

NANO ◽

10.1142/s1793292020500630 ◽

2020 ◽

Vol 15 (05) ◽

pp. 2050063

Author(s):

Zhifeng Cai ◽

Xiu Yin ◽

Jingling Fang ◽

Jie Zhao ◽

Tianqi Wu ◽

...

Keyword(s):

Fluorescence Intensity ◽

Photoelectron Spectroscopy ◽

Water Solubility ◽

Fluorescence Probe ◽

Synthesis Method ◽

Fluorescence Emission ◽

Average Diameter ◽

High Dispersion ◽

Excitation Wavelength ◽

One Pot

In this contribution, a one-pot synthesis method possessing the advantages of simple, green and low-cost had been researched for the preparation of L-histidine-stabilized Cu nanoclusters (Cu NCs). Subsequently, the structure and optical properties of as-prepared Cu NCs were studied by using Fourier transform infrared (FTIR), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), fluorescence spectroscopy and UV-Vis absorption spectroscopy. TEM image of the Cu NCs showed high dispersion with an average diameter of 2.0[Formula: see text]nm. Fluorescence spectrum displayed that the Cu NCs emitted green fluorescence (emission wavelength of 492[Formula: see text]nm) under excitation wavelength of 393[Formula: see text]nm. Moreover, the as-synthesized Cu NCs illustrated excellent performances, such as good water solubility, UV stability and high-salt resistance. Interestingly, the fluorescence intensity of as-prepared Cu NCs was obviously quenched in the presence of fluazinam. Under optimal conditions, the relative fluorescence intensity was linear with the fluazinam concentrations from 1 to 40[Formula: see text][Formula: see text]M, with a detection limit of 0.25[Formula: see text][Formula: see text]M. Eventually, the fluorescence sensor was successfully used to determine fluazinam in real water samples.

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Halothane and Isoflurane Alter the Calcium sup 2+ Binding Properties of Calmodulin

Anesthesiology ◽

10.1097/00000542-199507000-00015 ◽

1995 ◽

Vol 83 (1) ◽

pp. 120-126. ◽

Cited By ~ 17

Author(s):

Aaron Levin ◽

Thomas J. J. Blanck

Keyword(s):

Binding Protein ◽

Fluorescence Emission ◽

Volatile Anesthetic ◽

Volatile Anesthetics ◽

Bovine Brain ◽

Hill Coefficient ◽

Excitation Wavelength ◽

Binding Properties ◽

Fluorescence Measurements ◽

The Hill

Background Ca2+ plays an important role in signal transduction and anesthetic mechanisms. To date, no one has observed a direct effect of volatile anesthetics on a Ca(2+)-binding protein. We therefore examined the effects of halothane and isoflurane on the Ca(2+)-binding properties of bovine brain calmodulin. Methods The fluorescence emission of calmodulin was obtained over a range of Ca2+ concentrations (10(-7)-10(-4)M) in the presence and absence of halothane and isoflurane. The intrinsic tyrosine fluorescence of calmodulin was measured at an excitation wavelength of 280 nm and an emission wavelength of 320 nm. Fluorescence measurements were carried out in 50 mM hydroxyethylpiperazineethane sulfonic acid, 100 mM KC1, and 2 mM ethyleneglycol-bis-(beta-aminoethyl ether) tetraacetic acid at pH 7.0 and 37 degrees C. Experiments were performed in polytetrafluorethylene-sealed cuvettes so that the volatile anesthetic concentrations remained constant. The titration data were analyzed in two ways. The data were fit to the Hill equation by using nonlinear regression analysis to derive the Hill coefficient and the dissociation constant. The data were also analyzed by two-way analysis of variance with multiple comparisons to determine statistically significant effects. Volatile anesthetic concentrations were measured by gas chromatography. Results The presence of volatile anesthetics altered the Ca(2+)-binding affinity of calmodulin in a dose-dependent fashion. At 0.57% (0.25 mM) halothane and 1.7% (0.66 mM) isoflurane, the affinity of calmodulin for Ca2+ relative to control was decreased. However, at higher concentrations of both anesthetics, the affinity for Ca2+ was increased. When the volatile anesthetics were allowed to evaporate from the experimental solutions, the observed rightward shift of the calmodulin-Ca2+ binding curve for Ca2+ at low concentrations of the anesthetics returned to the control position. The leftward shift seen at high concentrations of the anesthetics was irreversible after evaporation of 8.7% (3.3 mM) isoflurane and 5.7% (2.5 mM) halothane. Conclusions These data demonstrate a complex interaction of two hydrophobic volatile anesthetics with calmodulin. A biphasic effect was observed both for halothane and for isoflurane. Calmodulin, an EF-hand Ca(2+)-binding protein, undergoes a conformational shift when binding Ca2+, exposing several hydrophobic residues. These residues may be sites at which the anesthetics act.

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Investigation of Excitation Wavelength for Fluorescence Emission of Citrus Peels based on UV-VIS Spectra

Engineering in Agriculture Environment and Food ◽

10.1016/s1881-8366(12)80008-5 ◽

2012 ◽

Vol 5 (4) ◽

pp. 126-132 ◽

Cited By ~ 10

Author(s):

Md. Abdul Momin ◽

Naoshi Kondo ◽

Makoto Kuramoto ◽

Yuichi Ogawa ◽

Kazuya Yamamoto ◽

...

Keyword(s):

Fluorescence Emission ◽

Excitation Wavelength ◽

Citrus Peels

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A Novel Near-Infrared Fluorescence Sensor for H2O2 Based on N-Acetyl-L-Cysteine-Capped Gold Nanoparticles

Journal of Nano Research ◽

10.4028/www.scientific.net/jnanor.46.234 ◽

2017 ◽

Vol 46 ◽

pp. 234-240

Author(s):

Wen Juan Dong ◽

Ji Yan Han ◽

Xin Wu ◽

Li Fan ◽

Wen Ting Liang

Keyword(s):

Hydrogen Peroxide ◽

Gold Nanoparticles ◽

Fluorescence Quenching ◽

Near Infrared ◽

Fluorescence Probe ◽

Fluorescence Emission ◽

Excitation Wavelength ◽

Experimental Conditions ◽

Time Resolved ◽

Near Infrared Fluorescence

A novel near-infrared fluorescence quenching method has been developed for the determination of hydrogen peroxide based on N-acetyl-L-cysteine-capped gold nanoparticles (NAC-AuNPs) as a fluorescence probe. The prepared gold nanoparticles with the size of about 1.91 nm exhibited strong near-infrared fluorescence emission at 693 nm with excitation wavelength at 450 nm in aqueous solution. The fluorescence intensity of NAC-AuNPs was quenched dramatically by adding hydrogen peroxide. Therefore, it could be used to detect hydrogen peroxide based on the fluorescence quenching intensity was linear with the concentration of hydrogen peroxide. Under the optimal experimental conditions, the linear range and detection limit were 1.0×10-6 –3.0×10-2 mol/L and 1.0×10-7 mol/L, respectively. The possible quenching mechanism was investigated by time-resolved fluorescence spectroscopy. The proposed method was simple, sensitive and showed good repeatability and stability.

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Spectroscopic Studies on the Interaction between Tilorone and Human Serum Albumin

French-Ukrainian Journal of Chemistry ◽

10.17721/fujcv5i1p48-59 ◽

2017 ◽

Vol 5 (1) ◽

pp. 48-59

Author(s):

Alla Yegorova ◽

Inna Leonenko ◽

Yulia Scrypynets ◽

Georgy Maltsev ◽

Valery Antonovich ◽

...

Keyword(s):

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Average Distance ◽

Antiviral Drug ◽

Fluorescence Emission ◽

Resonance Energy ◽

Spectroscopic Studies ◽

Excitation Wavelength

Under physiological conditions, in vitro interaction between the antiviral drug 2,7-bis[2-(diethylamino)ethoxy]-9-fluorenone dihydrochloride (Tilorone, TIL) and human serum albumin (HSA) was investigated at excitation wavelength 280 nm and at different temperatures (298 K and 313 K) by fluorescence emission spectroscopy. TIL showed a strong ability to quench the intrinsic fluorescence of HSA through a static quenching procedure. The binding constant is estimated as KA =7.19× 104L·mol-1 at 298 K. The enthalpy change (ΔHº) and entropy change (ΔSº) were derived to be negative values. A value of 1.63 nm for the average distance r between TIL (acceptor) and tryptophan residues of HSA (donor) was derived from the fluorescence resonance energy transfer.

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White-light emission from a structurally simple hydrazone

Chemical Communications ◽

10.1039/c9cc03912k ◽

2019 ◽

Vol 55 (64) ◽

pp. 9551-9554 ◽

Cited By ~ 8

Author(s):

Baihao Shao ◽

Nell Stankewitz ◽

Jacob A. Morris ◽

Matthew D. Liptak ◽

Ivan Aprahamian

Keyword(s):

White Light ◽

Light Emission ◽

Fluorescence Emission ◽

White Light Emission ◽

Excitation Wavelength ◽

Dual Fluorescence

Two hydrazones featuring a unique excitation wavelength-dependent dual fluorescence emission have been developed.

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A Novel Approach for In Vivo Measurement of Red Cell Redox Status

Blood ◽

10.1182/blood.v116.21.2036.2036 ◽

2010 ◽

Vol 116 (21) ◽

pp. 2036-2036 ◽

Cited By ~ 1

Author(s):

Xiuling Xu ◽

Katharina von Loehneysen ◽

Deborah Noack ◽

Andrew Vu ◽

Jeff S. Friedman

Keyword(s):

Fluorescence Emission ◽

Redox Status ◽

Red Cells ◽

Cysteine Residues ◽

Excitation Wavelength ◽

Red Cell ◽

Reducing Conditions ◽

Cell Redox Status

Abstract Abstract 2036 Maintenance of a reducing redox balance is a critical physiologic function of red cell metabolic machinery. Perturbation of this balance, whether inherited or acquired, is found in a variety of red cell pathologies. Methods for evaluation of red cell redox status include direct approaches such as determining glutathione (GSH, GSSG) levels, and indirect approaches such as measuring fluorescence of oxidation sensitive dyes. Here we describe an alternative method for evaluation of red cell redox status that can be used in vivo and in real-time assays. Engineered variants of GFP possessing two solvent accessible cysteine residues function as molecular redox sensors with distinct fluorescence characteristics. Excitation spectrum shifts upon the oxidation of cysteine residues forming a disulfide. A higher ratio of fluorescence when comparing excitation at 405nm versus 488nm indicates rising levels of oxidized GFP and a shift in cellular redox status. To validate redox GFPs in erythroid cells, we first performed in vitro assays with MEL cells over-expressing several related GFP sensors (ro-GFPs), selecting the brightest molecule (roGFP2) for further study. The sensor function of roGFP2 in MEL cells was verified by stimulation with exogenous oxidant (1mM H202) or reductant (10 mM DTT) as shown in the figure below. In order to create a physiologic in vivo model for study of red cell redox status, transgenic mice expressing roGFP2 specifically in the erythroid lineage were generated. roGFP2 expressing red cells demonstrate the expected shift in fluorescence upon exposure to H202 or t-butyl peroxide in a short-term assay. In vivo, we have measured red cell lifespan (using biotin-labeling) in roGFP2 transgenic animals to follow redox status of red cells as a function of cell age. Expression of roGFP2 has no effect on red cell survival. Interestingly, when comparing old red cells (age > 50days) with younger cells (age < 50days), a shift in GFP fluorescence ratio indicating that a higher fraction of the sensor is oxidized in the aged cells was observed. This observation is consistent with the hypothesis that metabolic changes, in particular a decline in ability to reduce oxidative damage, contribute to red cell senescence. We are generating several murine strains with defined red cell defects also expressing roGFP2 in order to assess the role of changes in intra-erythrocyte redox status in a range of pathologic conditions. In vitro and in vivo assays using roGFP2 transgenic cells/mice are in process to determine the potential utility of this system as a screen for hematoxicity of drugs and other compounds. Figure 1 Evaluation of roGFP2 function in MEL cells. The Y-axis shows fluorescence emission as a function of excitation wavelength (X-axis)—showing a shift when cells are exposed to oxidizing or reducing conditions. Figure 1. Evaluation of roGFP2 function in MEL cells. The Y-axis shows fluorescence emission as a function of excitation wavelength (X-axis)—showing a shift when cells are exposed to oxidizing or reducing conditions. Disclosures: No relevant conflicts of interest to declare.

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The photodynamic therapy activity of 3-(1-hydroxylethyl)-3-devinyl-131-(dicyanomethylene) pyropheophorbide-a methyl ester (HDCPPa) against HeLa cell in vitro

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424617500584 ◽

2017 ◽

Vol 21 (09) ◽

pp. 589-598 ◽

Cited By ~ 2

Author(s):

Wenting Li ◽

Qi Wang ◽

Guanghui Tan ◽

Hongyue Zhang ◽

Jianjun Cheng ◽

...

Keyword(s):

Photodynamic Therapy ◽

Methyl Ester ◽

Hela Cells ◽

High Stability ◽

Fluorescence Emission ◽

Ultraviolet Absorption Spectrum ◽

Excitation Wavelength ◽

Fluorescence Emission Spectrum ◽

Pyropheophorbide A

Photodynamic therapy (PDT) has been a potential therapeutic method for the treatment of various cancers, with photosensitizer being the key component in photodynamic therapy. In this paper, we prepared a photosensitizer 3-(1-hydroxylethyl)-3-devinyl-131-(dicyanomethylene) pyropheophorbide-a methyl ester (HDCPPa), based on chlorophyll pyropheophorbide-a according to the previous report, and systematically investigated the fluorescence emission spectrum and ultraviolet absorption spectrum HDCPPa has long absorption in the near-infrared spectral region (around 695 nm). The excitation wavelength and the emission wavelength were 415 nm and 699 nm respectively in dichloromethane, 1O2 quantum yield was 63.5%. HDCPPa also had high stability in PBS solution, DMEM cell culture medium and normal saline (NS) in vitro. After irradiation by the light of 675 nm (10 J.cm[Formula: see text]) for 70 min the degradation rate of HDCPPa was 12.5%, which indicated that the target compound showed high stability under light. The in vitrophotodynamic therapy activities against HeLa cells were also studied, which showed that HDCPPa had extremely low dark toxicity but great phototoxicity, and the cell viability is lower than 10% under the light irradiation of 675 nm (10 J.cm[Formula: see text]). Moreover, HDCPPa can quickly enter the cell after being incubated with HeLa cells in less than 30 min. We also evaluated the mechanism of the photochemical reaction, which had proved that Type II is primarily responsible for the cell death. Therefore HDCPPa could serve as a very promising photosensitizer for photodynamic therapy.

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