Background and Objectives:
Probiotics are nonpathogenic and beneficial viable microorganisms
that exhibit potential health welfare for human beings. Probiotics are found in various food
products. They also occur as natural microflora in the intestine of mammals. Main goal of this
study was to isolate probiotics conferring antibacterial activity and cholesterol lowering ability
from different fruits.
Materials and Method:
Present research reveals the usefulness of probiotics, in which twenty
one bacterial cultures were isolated from different fruit samples including figs, coconut water and
grapes. These strains were explored for their antibacterial and cholesterol reduction ability by
conducting in vitro experiments.
Results and Conclusion:
Among twenty one isolates, nine probiotic cultures FgC2, FgC7, FgC14,
G2C5, G1C,GrC18 and StCW showed maximum antibacterial activity against different human
clinical pathogens. This suggests that these microbes produce inhibitory metabolites which are
extracellular and diffusible. For cholesterol assimilation assay, six strains FgC2, FgC7,FgC12,
FgC13, GrC7 and GrC18 presented remarkable cholesterol lowering efficacy (up to 98%) when
grown in the presence of bile salts. Only potential probiotic cultures were identified and
characterized as lactic acid bacteria (LAB), on the basis of Bergey’s Manual of Determinative
Bacteriology. Thus this study is helpful to exploit the bioactive and therapeutic potential of
beneficial microorganisms so that they can be utilized in the generation of functional food and
other health promoting products.
ISOLATION OF ANTIBACTERIAL PROTEIN/PEPTIDE FROM FICUS GLOMERATA LEAF
